+Open data
-Basic information
Entry | Database: PDB / ID: 5lgm | ||||||
---|---|---|---|---|---|---|---|
Title | Gp5.7 mutant L42A | ||||||
Components | Fusion protein 5.5/5.7 | ||||||
Keywords | TRANSCRIPTION / Transcription effector / anti-sigma effector | ||||||
Function / homology | Protein suppressor of silencing, bacteriophage T7 / Bacteriophage T7, gene 5.5 / Fusion protein 5.5/5.7 Function and homology information | ||||||
Biological species | Enterobacteria phage T7 (virus) | ||||||
Method | SOLUTION NMR / distance geometry / simulated annealing | ||||||
Authors | Liu, B. / Matthews, S. | ||||||
Citation | Journal: to be published Title: Gp5.7 mutant L42A Authors: Liu, B. / Shadrin, A. / Sheppard, C. / Mekler, V. / Xu, Y. / Severinov, K. / Matthews, S. / Wigneshweraraj, S. #1: Journal: Nucleic Acids Res. / Year: 2014 Title: A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma factor displacement. Authors: Liu, B. / Shadrin, A. / Sheppard, C. / Mekler, V. / Xu, Y. / Severinov, K. / Matthews, S. / Wigneshweraraj, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 5lgm.cif.gz | 222.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb5lgm.ent.gz | 195.1 KB | Display | PDB format |
PDBx/mmJSON format | 5lgm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lg/5lgm ftp://data.pdbj.org/pub/pdb/validation_reports/lg/5lgm | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 7379.427 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T7 (virus) / Gene: 5.5 Production host: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others) References: UniProt: P03787 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution Contents: 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O Label: 15N_13C_gp5.7 / Solvent system: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | Ionic strength: na Not defined / Label: condition1 / pH: 6 / Pressure: 1 bar / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement |
| |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 10 / Conformers submitted total number: 10 |