+Open data
-Basic information
Entry | Database: PDB / ID: 1he8 | ||||||
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Title | Ras G12V - PI 3-kinase gamma complex | ||||||
Components |
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Keywords | KINASE/HYDROLASE / COMPLEX (PHOSPHOINOSITIDE KINASE-RAS) / PHOSPHOINOSITIDE 3-KINASE GAMMA - H-RAS G12V COMPLEX / PHOSPHATIDYLINOSITOL 3-KINASE / PI3K / PI 3-K / PI 3-KINASE / SECOND MESSENGER GENERATION / RAS EFFECTOR / H-RAS G12V / ONCOGENE PROTEIN / GMPPNP / GTP / RAS-BINDING DOMAIN / HEAT DOMAIN / KINASE-HYDROLASE complex | ||||||
Function / homology | Function and homology information negative regulation of triglyceride catabolic process / secretory granule localization / natural killer cell chemotaxis / neutrophil extravasation / phosphatidylinositol-4-phosphate 3-kinase / positive regulation of acute inflammatory response / respiratory burst involved in defense response / negative regulation of cardiac muscle contraction / regulation of calcium ion transmembrane transport / T cell chemotaxis ...negative regulation of triglyceride catabolic process / secretory granule localization / natural killer cell chemotaxis / neutrophil extravasation / phosphatidylinositol-4-phosphate 3-kinase / positive regulation of acute inflammatory response / respiratory burst involved in defense response / negative regulation of cardiac muscle contraction / regulation of calcium ion transmembrane transport / T cell chemotaxis / phospholipase C activator activity / negative regulation of fibroblast apoptotic process / phosphatidylinositol 3-kinase complex, class IB / GTPase complex / sphingosine-1-phosphate receptor signaling pathway / phosphatidylinositol 3-kinase complex, class IA / oncogene-induced cell senescence / positive regulation of ruffle assembly / dendritic cell chemotaxis / 1-phosphatidylinositol-4-phosphate 3-kinase activity / 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity / negative regulation of GTPase activity / phosphatidylinositol-4,5-bisphosphate 3-kinase / positive regulation of miRNA metabolic process / phosphatidylinositol 3-kinase / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / phosphatidylinositol-3-phosphate biosynthetic process / 1-phosphatidylinositol-3-kinase activity / T-helper 1 type immune response / positive regulation of wound healing / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / mast cell degranulation / hepatocyte apoptotic process / defense response to protozoan / positive regulation of Rac protein signal transduction / Signaling by RAS GAP mutants / Signaling by RAS GTPase mutants / regulation of cell adhesion mediated by integrin / Synthesis of PIPs at the plasma membrane / phosphatidylinositol-mediated signaling / Activation of RAS in B cells / phosphatidylinositol phosphate biosynthetic process / RAS signaling downstream of NF1 loss-of-function variants / SOS-mediated signalling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / SHC1 events in ERBB4 signaling / positive regulation of protein targeting to membrane / Signalling to RAS / SHC-related events triggered by IGF1R / Activated NTRK2 signals through FRS2 and FRS3 / adipose tissue development / regulation of angiogenesis / Estrogen-stimulated signaling through PRKCZ / SHC-mediated cascade:FGFR3 / MET activates RAS signaling / Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants / Signaling by PDGFRA extracellular domain mutants / SHC-mediated cascade:FGFR2 / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / Schwann cell development / phosphorylation / SHC-mediated cascade:FGFR4 / Signaling by FGFR4 in disease / Erythropoietin activates RAS / SHC-mediated cascade:FGFR1 / T cell proliferation / protein-membrane adaptor activity / FRS-mediated FGFR3 signaling / Signaling by FLT3 ITD and TKD mutants / FRS-mediated FGFR2 signaling / FRS-mediated FGFR4 signaling / cellular response to cAMP / Signaling by FGFR3 in disease / GPVI-mediated activation cascade / p38MAPK events / Tie2 Signaling / FRS-mediated FGFR1 signaling / Signaling by FGFR2 in disease / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / EPHB-mediated forward signaling / EGFR Transactivation by Gastrin / Signaling by FLT3 fusion proteins / FLT3 Signaling / ephrin receptor binding / neutrophil chemotaxis / Signaling by FGFR1 in disease / myelination / GRB2 events in ERBB2 signaling / CD209 (DC-SIGN) signaling / phosphatidylinositol 3-kinase/protein kinase B signal transduction / Ras activation upon Ca2+ influx through NMDA receptor / NCAM signaling for neurite out-growth / positive regulation of endothelial cell migration / SHC1 events in ERBB2 signaling / Downstream signal transduction / intrinsic apoptotic signaling pathway / Constitutive Signaling by Overexpressed ERBB2 / Insulin receptor signalling cascade Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Pacold, M.E. / Suire, S. / Perisic, O. / Lara-Gonzalez, S. / Davis, C.T. / Hawkins, P.T. / Walker, E.H. / Stephens, L. / Eccleston, J.F. / Williams, R.L. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2000 Title: Crystal Structure and Functional Analysis of Ras Binding to its Effector Phosphoinositide 3-Kinase Gamma Authors: Pacold, M.E. / Suire, S. / Perisic, O. / Lara-Gonzalez, S. / Davis, C.T. / Walker, E.H. / Hawkins, P.T. / Stephens, L. / Eccleston, J.F. / Williams, R.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1he8.cif.gz | 205.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1he8.ent.gz | 157 KB | Display | PDB format |
PDBx/mmJSON format | 1he8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1he8_validation.pdf.gz | 796.8 KB | Display | wwPDB validaton report |
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Full document | 1he8_full_validation.pdf.gz | 829.9 KB | Display | |
Data in XML | 1he8_validation.xml.gz | 36.8 KB | Display | |
Data in CIF | 1he8_validation.cif.gz | 49.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/he/1he8 ftp://data.pdbj.org/pub/pdb/validation_reports/he/1he8 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | HETERODIMER OF A 101 KDA SUBUNIT AND A 120 KDA CATALYTICSUBUNIT |
-Components
#1: Protein | Mass: 110626.953 Da / Num. of mol.: 1 / Fragment: P110 GAMMA CATALYTIC SUBUNIT / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PVL1393 / Cell line (production host): Sf9 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: P48736, phosphatidylinositol 3-kinase |
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#2: Protein | Mass: 18917.271 Da / Num. of mol.: 1 / Fragment: EFFECTOR DOMAIN / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PET-11A / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P23175, UniProt: P01112*PLUS |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-GNP / |
#5: Water | ChemComp-HOH / |
Compound details | CHAIN A: PHOSPHATIDYLINOSITOL 3-KINASE, 3-PHOSPHORYLATES THE CELLULAR PHOSPHOINOSITIDE PTDINS-4,5- ...CHAIN A: PHOSPHATID |
Sequence details | CHAIN A, P48736: IN THE STRUCTURE, A528-A619 IN THE HELICAL DOMAIN WAS PROBABLY LOST TO PROTEOLYSIS ...CHAIN A, P48736: IN THE STRUCTURE, A528-A619 IN THE HELICAL DOMAIN WAS PROBABLY LOST TO PROTEOLYSI |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 64 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.3 / Details: 50 MM KH2PO4 PH 6.3, 4.3% PEG 8000 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / pH: 6.3 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 15, 2000 / Details: GE(220) AND MULTILAYER |
Radiation | Monochromator: DIAMOND C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. obs: 25822 / % possible obs: 91.6 % / Redundancy: 9 % / Biso Wilson estimate: 82.3 Å2 / Rmerge(I) obs: 0.102 / Net I/σ(I): 20 |
Reflection shell | Resolution: 3→3.19 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.301 / Mean I/σ(I) obs: 1.7 / % possible all: 50.5 |
Reflection | *PLUS Num. measured all: 232825 |
Reflection shell | *PLUS % possible obs: 50.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRIES 1E8Y AND 5P21 Resolution: 3→52.03 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 2257126.69 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 Details: RESIDUES 1085 - 1108 ARE NOT VISIBLE IN THE DENSITY.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 76.7474 Å2 / ksol: 0.384614 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 70.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→52.03 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.19 Å / Rfactor Rfree error: 0.032 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.28 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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