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- PDB-3rt0: Crystal structure of PYL10-HAB1 complex in the absence of abscisi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3rt0 | ||||||
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Title | Crystal structure of PYL10-HAB1 complex in the absence of abscisic acid (ABA) | ||||||
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![]() | HYDROLASE/HYDROLASE INHIBITOR / PYL10-HAB1 binary COMPLEX / apo-PYL10 inhibits HAB1 dephosphorylation activity / HYDROLASE-HYDROLASE INHIBITOR complex | ||||||
Function / homology | ![]() regulation of protein serine/threonine phosphatase activity / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / signaling receptor activity / protein homodimerization activity / nucleus ...regulation of protein serine/threonine phosphatase activity / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / signaling receptor activity / protein homodimerization activity / nucleus / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hao, Q. / Yin, P. / Li, W. / Wang, L. / Yan, C. / Wang, J. / Yan, N. | ||||||
![]() | ![]() Title: The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins Authors: Hao, Q. / Yin, P. / Li, W. / Wang, L. / Yan, C. / Lin, Z. / Wu, J.Z. / Wang, J. / Yan, S.F. / Yan, N. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 407.5 KB | Display | ![]() |
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PDB format | ![]() | 332.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464 KB | Display | ![]() |
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Full document | ![]() | 487.8 KB | Display | |
Data in XML | ![]() | 43.5 KB | Display | |
Data in CIF | ![]() | 61.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3rt2SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37753.219 Da / Num. of mol.: 2 / Fragment: residues 172- 511 / Mutation: C274S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q9CAJ0, protein-serine/threonine phosphatase #2: Protein | Mass: 20658.545 Da / Num. of mol.: 2 / Mutation: C166S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.64 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100mM Tris, 21% PEG3350, 2% Dioxane , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Oct 27, 2010 |
Radiation | Monochromator: rotated-inclined double-crystal monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→35 Å / Num. obs: 58037 / % possible obs: 99.8 % / Biso Wilson estimate: 25.43 Å2 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.399 / Mean I/σ(I) obs: 4.16 / Num. unique all: 5772 / Rsym value: 0.399 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3RT2 Resolution: 2.113→32.499 Å / Occupancy max: 1 / Occupancy min: 0.12 / FOM work R set: 0.8629 / SU ML: 0.24 / σ(F): 0 / Phase error: 21.01 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 41.02 Å2 / ksol: 0.338 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 203.27 Å2 / Biso mean: 36.291 Å2 / Biso min: 9.77 Å2
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Refinement step | Cycle: LAST / Resolution: 2.113→32.499 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 37.9109 Å / Origin y: 15.0621 Å / Origin z: 12.3016 Å
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Refinement TLS group |
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