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- PDB-1h25: CDK2/Cyclin A in complex with an 11-residue recruitment peptide f... -
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Basic information
Entry | Database: PDB / ID: 1h25 | ||||||
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Title | CDK2/Cyclin A in complex with an 11-residue recruitment peptide from retinoblastoma-associated protein | ||||||
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![]() | TRANSFERASE / CELL CYCLE / PROTEIN KINASE / CYCLIN / CDK2 / RECRUITMENT / PEPTIDE SPECIFICITY | ||||||
Function / homology | ![]() Defective translocation of RB1 mutants to the nucleus / enucleate erythrocyte differentiation / positive regulation of collagen fibril organization / negative regulation of tau-protein kinase activity / Rb-E2F complex / regulation of lipid kinase activity / negative regulation of myofibroblast differentiation / maintenance of mitotic sister chromatid cohesion / cell morphogenesis involved in neuron differentiation / chromatin lock complex ...Defective translocation of RB1 mutants to the nucleus / enucleate erythrocyte differentiation / positive regulation of collagen fibril organization / negative regulation of tau-protein kinase activity / Rb-E2F complex / regulation of lipid kinase activity / negative regulation of myofibroblast differentiation / maintenance of mitotic sister chromatid cohesion / cell morphogenesis involved in neuron differentiation / chromatin lock complex / sister chromatid biorientation / Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes / cyclin A2-CDK1 complex / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / positive regulation of extracellular matrix organization / regulation of centromere complex assembly / cell cycle G1/S phase transition / Aberrant regulation of mitotic exit in cancer due to RB1 defects / cellular response to luteinizing hormone stimulus / positive regulation of macrophage differentiation / positive regulation of transcription regulatory region DNA binding / glial cell apoptotic process / tissue homeostasis / mitotic cell cycle phase transition / protein localization to chromosome, centromeric region / negative regulation of protein serine/threonine kinase activity / positive regulation of mitotic metaphase/anaphase transition / importin-alpha family protein binding / Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 / negative regulation of hepatocyte apoptotic process / neuron maturation / cellular response to leptin stimulus / digestive tract development / male pronucleus / female pronucleus / aortic valve morphogenesis / Replication of the SARS-CoV-1 genome / response to glucagon / cellular response to cocaine / myoblast differentiation / negative regulation of cold-induced thermogenesis / SWI/SNF complex / cyclin-dependent protein serine/threonine kinase regulator activity / cellular response to insulin-like growth factor stimulus / negative regulation of glial cell proliferation / smoothened signaling pathway / negative regulation of G1/S transition of mitotic cell cycle / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / positive regulation of DNA biosynthetic process / cochlea development / cyclin A1-CDK2 complex / cyclin E2-CDK2 complex / cyclin E1-CDK2 complex / cellular response to platelet-derived growth factor stimulus / cyclin A2-CDK2 complex / positive regulation of DNA-templated DNA replication initiation / G2 Phase / cyclin-dependent protein kinase activity / Y chromosome / Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes / hepatocyte apoptotic process / positive regulation of heterochromatin formation / p53-Dependent G1 DNA Damage Response / X chromosome / PTK6 Regulates Cell Cycle / skeletal muscle cell differentiation / regulation of DNA replication / RUNX2 regulates osteoblast differentiation / regulation of anaphase-promoting complex-dependent catabolic process / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / centriole replication / Regulation of APC/C activators between G1/S and early anaphase / telomere maintenance in response to DNA damage / centrosome duplication / negative regulation of apoptotic signaling pathway / G0 and Early G1 / Telomere Extension By Telomerase / negative regulation of cell cycle / Activation of the pre-replicative complex / cellular response to nitric oxide / chromosome organization / Regulation of MITF-M-dependent genes involved in cell cycle and proliferation / glial cell proliferation / cyclin-dependent kinase / animal organ regeneration / chondrocyte differentiation / cyclin-dependent protein serine/threonine kinase activity / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / Nuclear events stimulated by ALK signaling in cancer / Activation of ATR in response to replication stress / Cyclin E associated events during G1/S transition / Cyclin A/B1/B2 associated events during G2/M transition / Cajal body / cyclin-dependent protein kinase holoenzyme complex / Cyclin A:Cdk2-associated events at S phase entry / negative regulation of smoothened signaling pathway / condensed chromosome / mitotic G1 DNA damage checkpoint signaling / striated muscle cell differentiation / regulation of mitotic cell cycle Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tews, I. / Cheng, K.Y. / Lowe, E.D. / Noble, M.E.M. / Brown, N.R. / Gul, S. / Gamblin, S. / Johnson, L.N. | ||||||
![]() | ![]() Title: Specificity Determinants of Recruitment Peptides Bound to Phospho-Cdk2/Cyclin A Authors: Lowe, E.D. / Tews, I. / Cheng, K.Y. / Brown, N.R. / Gul, S. / Noble, M.E.M. / Gamblin, S. / Johnson, L.N. #1: ![]() Title: The Structural Basis for Specificity of Substrate and Recruitment Peptides for Cyclin-Dependant Kinases Authors: Brown, N.R. / Noble, M.E.M. / Endicott, J.A. / Johnson, L.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 230.1 KB | Display | ![]() |
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PDB format | ![]() | 185.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1h24C ![]() 1h26C ![]() 1h27C ![]() 1h28C ![]() 1qmzS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Details | BIOMOLECULE 1 IS A TRIMERIC COMPLEX OF CHAINS A, B AND E. BIOMOLECULE 2 IS A DIMERIC COMPLEX OF CHAINS C AND D |
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Components
#1: Protein | Mass: 34467.926 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P24941, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor #2: Protein | Mass: 29753.410 Da / Num. of mol.: 2 / Fragment: CYCLIN FOLD, RESIDUES 175-432 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein/peptide | | Mass: 1342.670 Da / Num. of mol.: 1 / Fragment: RESIDUES 869-878 / Source method: obtained synthetically / Source: (synth.) ![]() #4: Water | ChemComp-HOH / | Compound details | CDK2: CONTROL OF THE CELL CYCLE DURING S PHASE AND G2. BELONGS TO THE SER/THR FAMILY OF PROTEIN ...CDK2: CONTROL OF THE CELL CYCLE DURING S PHASE AND G2. BELONGS TO THE SER/THR FAMILY OF PROTEIN KINASES. CYCLIN A2: CONTROL OF THE CELL CYCLE. INTERACTS WITH THE CDK2 AND CDC2 PROTEIN KINASES. RETINOBLAS | Sequence details | CHAINS B AND D ARE A TRUNCATED FRAGMENT OF CYCLIN A2 CONSISTING | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 59.8 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 Details: 0.8M KCL, 1.2M (NH4)2SO4, 40MM HEPES PH 7.0. PROTEIN CONCENTRATION = 10MG/ML | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.4 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Nov 15, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→28.99 Å / Num. obs: 52141 / % possible obs: 100 % / Redundancy: 4.8 % / Rmerge(I) obs: 0.092 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.439 / Mean I/σ(I) obs: 1.7 / % possible all: 100 |
Reflection | *PLUS % possible obs: 100 % / Num. measured all: 252831 |
Reflection shell | *PLUS % possible obs: 100 % |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QMZ Resolution: 2.5→28 Å / SU B: 11.694 / SU ML: 0.257 / Cross valid method: THROUGHOUT / ESU R: 0.531 / ESU R Free: 0.291
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Displacement parameters | Biso mean: 55.509 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→28 Å
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Refinement | *PLUS Lowest resolution: 20 Å / Rfactor Rfree: 0.296 / Rfactor Rwork: 0.238 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 2.56 Å / Rfactor Rfree: 0.361 / Rfactor Rwork: 0.338 / Num. reflection Rwork: 194 / Total num. of bins used: 20 |