|Entry||Database: PDB / ID: 1fq1|
|Title||CRYSTAL STRUCTURE OF KINASE ASSOCIATED PHOSPHATASE (KAP) IN COMPLEX WITH PHOSPHO-CDK2|
|Keywords||Hydrolase/Transferase / phospho-protein-protein complex / Hydrolase-Transferase COMPLEX|
|Function / homology|
Function and homology information
protein tyrosine/serine/threonine phosphatase activity / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / cyclin A1-CDK2 complex / cyclin E2-CDK2 complex / cyclin E1-CDK2 complex / cyclin A2-CDK2 complex / positive regulation of DNA-templated DNA replication initiation / G2 Phase ...protein tyrosine/serine/threonine phosphatase activity / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / cyclin A1-CDK2 complex / cyclin E2-CDK2 complex / cyclin E1-CDK2 complex / cyclin A2-CDK2 complex / positive regulation of DNA-templated DNA replication initiation / G2 Phase / cyclin-dependent protein kinase activity / Y chromosome / Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes / X chromosome / positive regulation of heterochromatin formation / p53-Dependent G1 DNA Damage Response / PTK6 Regulates Cell Cycle / regulation of anaphase-promoting complex-dependent catabolic process / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / centriole replication / Regulation of APC/C activators between G1/S and early anaphase / centrosome duplication / Telomere Extension By Telomerase / G0 and Early G1 / cyclin-dependent kinase / Activation of the pre-replicative complex / cyclin-dependent protein serine/threonine kinase activity / cyclin-dependent protein kinase holoenzyme complex / cellular response to nitric oxide / Cyclin A/B1/B2 associated events during G2/M transition / Cyclin E associated events during G1/S transition / Cajal body / Cyclin A:Cdk2-associated events at S phase entry / Activation of ATR in response to replication stress / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / condensed chromosome / regulation of cyclin-dependent protein serine/threonine kinase activity / mitotic G1 DNA damage checkpoint signaling / post-translational protein modification / regulation of G2/M transition of mitotic cell cycle / histone modification / protein-tyrosine-phosphatase / meiotic cell cycle / response to organic substance / male germ cell nucleus / protein tyrosine phosphatase activity / cyclin binding / protein dephosphorylation / DNA Damage/Telomere Stress Induced Senescence / potassium ion transport / Meiotic recombination / CDK-mediated phosphorylation and removal of Cdc6 / SCF(Skp2)-mediated degradation of p27/p21 / G1/S transition of mitotic cell cycle / Orc1 removal from chromatin / Transcriptional regulation of granulopoiesis / Cyclin D associated events in G1 / G2/M transition of mitotic cell cycle / cellular senescence / Regulation of TP53 Degradation / nuclear envelope / Processing of DNA double-strand break ends / Factors involved in megakaryocyte development and platelet production / regulation of gene expression / Senescence-Associated Secretory Phenotype (SASP) / Ras protein signal transduction / Regulation of TP53 Activity through Phosphorylation / chromosome, telomeric region / transcription regulator complex / DNA replication / regulation of cell cycle / endosome / peptidyl-serine phosphorylation / cell division / negative regulation of cell population proliferation / centrosome / protein domain specific binding / DNA repair / DNA-templated transcription / protein serine kinase activity / protein serine/threonine kinase activity / positive regulation of cell population proliferation / protein phosphorylation / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / magnesium ion binding / signal transduction / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Cyclin-dependent kinase inhibitor 3 / CDKN3 domain / Cyclin-dependent kinase inhibitor 3 (CDKN3) / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Protein tyrosine phosphatase superfamily / Protein-Tyrosine Phosphatase; Chain A / Protein-tyrosine phosphatase, catalytic / Protein tyrosine phosphatase, catalytic domain motif / Tyrosine specific protein phosphatases domain profile. ...Cyclin-dependent kinase inhibitor 3 / CDKN3 domain / Cyclin-dependent kinase inhibitor 3 (CDKN3) / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Protein tyrosine phosphatase superfamily / Protein-Tyrosine Phosphatase; Chain A / Protein-tyrosine phosphatase, catalytic / Protein tyrosine phosphatase, catalytic domain motif / Tyrosine specific protein phosphatases domain profile. / Tyrosine-specific protein phosphatases domain / Protein-tyrosine phosphatase-like / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Alpha-Beta Complex / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-TRIPHOSPHATE / Cyclin-dependent kinase 2 / Cyclin-dependent kinase inhibitor 3
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3 Å|
|Authors||Song, H. / Hanlon, N. / Brown, N.R. / Noble, M.E.M. / Johnson, L.N. / Barford, D.|
Journal: Mol.Cell / Year: 2001
Title: Phosphoprotein-protein interactions revealed by the crystal structure of kinase-associated phosphatase in complex with phosphoCDK2.
Authors: Song, H. / Hanlon, N. / Brown, N.R. / Noble, M.E. / Johnson, L.N. / Barford, D.
#1: Journal: Science / Year: 1995
Title: Dephosphorylation of Cdk2 Thr160 by the cyclin dependent kinase-interacting phosphatase KAP in the absence of cyclin
Authors: Poon, R.Y.C. / Hunter, T.
#2: Journal: Cell(Cambridge,Mass.) / Year: 1993
Title: Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
Authors: Gyruris, J. / Golemis, E. / Chertkov, H. / Brent, R.
#3: Journal: Cell(Cambridge,Mass.) / Year: 1993
Title: The p21Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
Authors: Harper, J.W. / Adami, G.R. / Wei, N. / Keyomarsi, K. / Elledge, S.J.
|Structure viewer||Molecule: |
-Downloads & links
|PDBx/mmCIF format||1fq1.cif.gz||105.7 KB||Display||PDBx/mmCIF format|
|PDB format||pdb1fq1.ent.gz||85.4 KB||Display||PDB format|
|PDBx/mmJSON format||1fq1.json.gz||Tree view||PDBx/mmJSON format|
|Summary document||1fq1_validation.pdf.gz||580.2 KB||Display||wwPDB validaton report|
|Full document||1fq1_full_validation.pdf.gz||627.7 KB||Display|
|Data in XML||1fq1_validation.xml.gz||27.6 KB||Display|
-Related structure data
|Related structure data|
C: citing same article (ref.)
|Similar structure data|
|PDB pages||PDBj / wwPDB / NCBI|
|Related items in Molecule of the Month|
A: CYCLIN-DEPENDENT KINASE INHIBITOR 3
B: CELL DIVISION PROTEIN KINASE 2
|#1: Protein|| |
Mass: 23819.057 Da / Num. of mol.: 1 / Mutation: C140S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Description: HOMO SAPIENS / Production host: Escherichia coli (E. coli) / References: UniProt: Q16667, protein-tyrosine-phosphatase
|#2: Protein|| |
Mass: 34056.469 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Description: HOMO SAPIENS / Production host: Escherichia coli (E. coli) / References: UniProt: P24941, cyclin-dependent kinase
|#3: Chemical|| ChemComp-MG / |
|#4: Chemical|| ChemComp-ATP / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.96 Å3/Da / Density % sol: 58.52 %|
|Crystal grow||Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 |
Details: PEG 8000,KCl, Mgacetate,Na cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
*PLUSTemperature: 4 ℃
|Components of the solutions|
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: SYNCHROTRON / Site: ESRF / Beamline: BM14|
|Detector||Type: ADSC / Detector: CCD / Date: Jan 1, 2000|
|Radiation||Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Relative weight: 1|
|Reflection||Resolution: 3→30 Å / Num. obs: 17091 / % possible obs: 99.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.3 % / Biso Wilson estimate: 95.5 Å2 / Rmerge(I) obs: 0.073 / Net I/σ(I): 5.6|
|Reflection shell||Resolution: 3→3.13 Å / Redundancy: 5.3 % / Rmerge(I) obs: 0.36 / % possible all: 99.9|
*PLUSNum. measured all: 89741
*PLUS% possible obs: 99.6 % / Mean I/σ(I) obs: 1.8
|Refinement||Resolution: 3→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber|
|Refinement step||Cycle: LAST / Resolution: 3→20 Å|
|Refine LS restraints|
*PLUSName: CNS / Classification: refinement
*PLUSHighest resolution: 3 Å / Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 10 % / Rfactor obs: 0.235
|Refine LS restraints|
*PLUSType: c_angle_deg / Dev ideal: 1.8
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