|Entry||Database: PDB / ID: 1fq1|
|Title||CRYSTAL STRUCTURE OF KINASE ASSOCIATED PHOSPHATASE (KAP) IN COMPLEX WITH PHOSPHO-CDK2|
|Keywords||Hydrolase/Transferase / phospho-protein-protein complex / Hydrolase-Transferase COMPLEX|
|Function / homology||DNA Damage/Telomere Stress Induced Senescence / G2 Phase / Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes / Protein kinase domain / Activation of the pre-replicative complex / Protein-tyrosine phosphatase-like / CDKN3 domain / Protein kinase, ATP binding site / Protein kinase-like domain superfamily / Orc1 removal from chromatin ...DNA Damage/Telomere Stress Induced Senescence / G2 Phase / Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes / Protein kinase domain / Activation of the pre-replicative complex / Protein-tyrosine phosphatase-like / CDKN3 domain / Protein kinase, ATP binding site / Protein kinase-like domain superfamily / Orc1 removal from chromatin / Cyclin-dependent kinase inhibitor 3 / Protein kinases ATP-binding region signature. / Serine/threonine-protein kinase, active site / Processing of DNA double-strand break ends / Regulation of TP53 Degradation / Protein-tyrosine phosphatase, catalytic / Protein kinase domain / Tyrosine specific protein phosphatases domain / Regulation of TP53 Activity through Phosphorylation / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / Cyclin-dependent kinase inhibitor 3 (CDKN3) / CDK-mediated phosphorylation and removal of Cdc6 / Cyclin E associated events during G1/S transition / PTK6 Regulates Cell Cycle / Senescence-Associated Secretory Phenotype (SASP) / SCF(Skp2)-mediated degradation of p27/p21 / Regulation of APC/C activators between G1/S and early anaphase / Factors involved in megakaryocyte development and platelet production / Meiotic recombination / Serine/Threonine protein kinases active-site signature. / G0 and Early G1 / Activation of ATR in response to replication stress / Tyrosine specific protein phosphatases family profile. / Cyclin A/B1/B2 associated events during G2/M transition / Protein kinase domain profile. / Cyclin A:Cdk2-associated events at S phase entry / p53-Dependent G1 DNA Damage Response / protein tyrosine/serine/threonine phosphatase activity / protein-serine/threonine phosphatase / protein serine/threonine phosphatase activity / regulation of cyclin-dependent protein serine/threonine kinase activity / cyclin A1-CDK2 complex / cyclin E1-CDK2 complex / cyclin E2-CDK2 complex / cyclin A2-CDK2 complex / positive regulation of DNA-dependent DNA replication initiation / Y chromosome / cyclin-dependent protein kinase activity / X chromosome / histone phosphorylation / centrosome duplication / centriole replication / cyclin-dependent protein kinase holoenzyme complex / cyclin-dependent kinase / cyclin-dependent protein serine/threonine kinase activity / Cajal body / condensed chromosome / cellular response to nitric oxide / cyclin binding / mitotic G1 DNA damage checkpoint / regulation of gene silencing / potassium ion transport / meiotic cell cycle / protein-tyrosine-phosphatase / chromosome, telomeric region / protein tyrosine phosphatase activity / G1/S transition of mitotic cell cycle / anaphase-promoting complex-dependent catabolic process / cell cycle arrest / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / Ras protein signal transduction / regulation of G2/M transition of mitotic cell cycle / transcription factor complex / G2/M transition of mitotic cell cycle / regulation of signal transduction by p53 class mediator / DNA replication / endosome / peptidyl-serine phosphorylation / centrosome / cell division / negative regulation of cell population proliferation / DNA repair / protein domain specific binding / protein serine/threonine kinase activity / protein phosphorylation / positive regulation of cell population proliferation / positive regulation of transcription, DNA-templated / negative regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / magnesium ion binding / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm / Cyclin-dependent kinase 2 / Cyclin-dependent kinase inhibitor 3|
Function and homology information
|Specimen source||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / 3 Å resolution|
|Authors||Song, H. / Hanlon, N. / Brown, N.R. / Noble, M.E.M. / Johnson, L.N. / Barford, D.|
Journal: Mol.Cell / Year: 2001
Title: Phosphoprotein-protein interactions revealed by the crystal structure of kinase-associated phosphatase in complex with phosphoCDK2.
Authors: Song, H. / Hanlon, N. / Brown, N.R. / Noble, M.E. / Johnson, L.N. / Barford, D.
#1: Journal: Science / Year: 1995
Title: Dephosphorylation of Cdk2 Thr160 by the cyclin dependent kinase-interacting phosphatase KAP in the absence of cyclin
Authors: Poon, R.Y.C. / Hunter, T.
#2: Journal: Cell(Cambridge,Mass.) / Year: 1993
Title: Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
Authors: Gyruris, J. / Golemis, E. / Chertkov, H. / Brent, R.
SummaryFull reportAbout validation report
|Date||Deposition: Sep 1, 2000 / Release: May 9, 2001|
|Structure viewer||Molecule: |
Downloads & links
A: CYCLIN-DEPENDENT KINASE INHIBITOR 3
B: CELL DIVISION PROTEIN KINASE 2
|#1: Protein/peptide|| |
Mass: 23819.057 Da / Num. of mol.: 1 / Mutation: C140S / Source: (gene. exp.) Homo sapiens (human) / Genus: Homo / Description: HOMO SAPIENS / Genus (production host): Escherichia / Production host: Escherichia coli (E. coli) / References: UniProt: Q16667, protein-tyrosine-phosphatase
|#2: Protein/peptide|| |
Mass: 34056.469 Da / Num. of mol.: 1 / Source: (gene. exp.) Homo sapiens (human) / Genus: Homo / Description: HOMO SAPIENS / Genus (production host): Escherichia / Production host: Escherichia coli (E. coli) / References: UniProt: P24941, cyclin-dependent kinase
|#3: Chemical|| ChemComp-MG / |
|#4: Chemical|| ChemComp-ATP / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.96 / Density percent sol: 58.52 %|
|Crystal grow||Temp: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5|
Details: PEG 8000,KCl, Mgacetate,Na cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
*PLUSTemp: 4 ℃
|components of the solutions|
|Diffraction||Mean temperature: 100|
|Source||Source: SYNCHROTRON / Type: ESRF BEAMLINE BM14 / Synchrotron site: ESRF / Beamline: BM14|
|Detector||Type: ADSC / Detector: CCD / Collection date: Jan 1, 2000|
|Radiation||Diffraction protocol: SINGLE WAVELENGTH / Monochromatic or laue m l: M / Scattering type: x-ray|
|Radiation wavelength||Relative weight: 1|
|Reflection||B iso Wilson estimate: 95.5 / D resolution high: 3 / D resolution low: 3 / Number obs: 17091 / Observed criterion sigma F: 2 / Observed criterion sigma I: 2 / Rmerge I obs: 0.073 / NetI over sigmaI: 5.6 / Redundancy: 5.3 % / Percent possible obs: 99.7|
|Reflection shell||Rmerge I obs: 0.36 / Highest resolution: 3 / Lowest resolution: 3.13 / Redundancy: 5.3 % / Percent possible all: 99.9|
*PLUSNumber measured all: 89741
*PLUSPercent possible obs: 99.6 / MeanI over sigI obs: 1.8
|Refine||R Free selection details: RANDOM / Sigma F: 0 / Sigma I: 0 / Stereochemistry target values: Engh & Huber|
|Least-squares process||R factor R free: 0.313 / R factor R work: 0.235 / Highest resolution: 3 / Lowest resolution: 2 / Number reflection R free: 702 / Number reflection obs: 13940 / Percent reflection obs: 99.7|
|Refine hist #LAST||Highest resolution: 3 / Lowest resolution: 2|
|Number of atoms included #LAST||Protein: 3822 / Nucleic acid: 0 / Ligand: 32 / Solvent: 0 / Total: 3854|
|Refine LS restraints|
*PLUSName: CNS / Classification: refinement
*PLUSSigma F: 0
*PLUSR factor obs: 0.235 / Highest resolution: 3 / Lowest resolution: 2 / Percent reflection R free: 1
|Refine LS restraints|
*PLUSType: c_angle_deg / Dev ideal: 1.8
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