+Open data
-Basic information
Entry | Database: PDB / ID: 1gy6 | ||||||
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Title | NTF2 from rat, ammonium sulphate conditions | ||||||
Components | NUCLEAR TRANSPORT FACTOR 2 | ||||||
Keywords | NUCLEAR TRANSPORT | ||||||
Function / homology | Function and homology information negative regulation of vascular endothelial growth factor production / protein localization to nuclear pore / nuclear pore central transport channel / structural constituent of nuclear pore / nuclear outer membrane / nucleocytoplasmic transport / nuclear inner membrane / nuclear import signal receptor activity / mRNA transport / protein export from nucleus ...negative regulation of vascular endothelial growth factor production / protein localization to nuclear pore / nuclear pore central transport channel / structural constituent of nuclear pore / nuclear outer membrane / nucleocytoplasmic transport / nuclear inner membrane / nuclear import signal receptor activity / mRNA transport / protein export from nucleus / small GTPase binding / positive regulation of protein import into nucleus / protein import into nucleus / nuclear membrane / extracellular exosome / nucleoplasm / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | RATTUS NORVEGICUS (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Bayliss, R. / Stewart, M. | ||||||
Citation | Journal: Embo J. / Year: 2002 Title: Structural Basis for the Interaction between Ntf2 and Nucleoporin Fxfg Repeats Authors: Bayliss, R. / Leung, S. / Baker, R. / Quimby, B. / Corbett, A. / Stewart, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gy6.cif.gz | 63.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gy6.ent.gz | 47.1 KB | Display | PDB format |
PDBx/mmJSON format | 1gy6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gy/1gy6 ftp://data.pdbj.org/pub/pdb/validation_reports/gy/1gy6 | HTTPS FTP |
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-Related structure data
Related structure data | 1gy5C 1gy7C 1gybC 1ounS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14491.425 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P13662, UniProt: P61972*PLUS #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.51 % |
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Crystal grow | pH: 5.5 Details: 100MM AMMONIUM ACETATE PH 5.5, 1.2M AMMONIUM SULPHATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: MARRESEARCH |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→36.3 Å / Num. obs: 36958 / % possible obs: 98.6 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.042 / Net I/σ(I): 24.6 |
Reflection shell | Resolution: 1.6→1.69 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.19 / Mean I/σ(I) obs: 4.6 / % possible all: 93.5 |
Reflection | *PLUS Num. measured all: 234160 |
Reflection shell | *PLUS % possible obs: 93.5 % / Rmerge(I) obs: 0.19 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1OUN Resolution: 1.6→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.6→20 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 20 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.5 |