+Open data
-Basic information
Entry | Database: PDB / ID: 1gy7 | ||||||
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Title | N77Y point mutant of S.Cerevisiae NTF2 | ||||||
Components | NUCLEAR TRANSPORT FACTOR 2 | ||||||
Keywords | NUCLEAR TRANSPORT / NUCLEAR TRANSPORT FACTOR / PROTEIN TRANSPORT | ||||||
Function / homology | Function and homology information nuclear pore central transport channel / nucleocytoplasmic transport / nuclear import signal receptor activity / small GTPase binding / protein import into nucleus / nuclear envelope / cytoplasm Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Bayliss, R. / Quimby, B.B. / Corbett, A. / Stewart, M. | ||||||
Citation | Journal: Embo J. / Year: 2002 Title: Structural Basis for the Interaction between Ntf2 and Nucleoporin Fxfg Repeats Authors: Bayliss, R. / Leung, S. / Baker, R. / Quimby, B.B. / Corbett, A. / Stewart, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gy7.cif.gz | 113.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gy7.ent.gz | 89.3 KB | Display | PDB format |
PDBx/mmJSON format | 1gy7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1gy7_validation.pdf.gz | 378 KB | Display | wwPDB validaton report |
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Full document | 1gy7_full_validation.pdf.gz | 385.5 KB | Display | |
Data in XML | 1gy7_validation.xml.gz | 10.1 KB | Display | |
Data in CIF | 1gy7_validation.cif.gz | 18.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gy/1gy7 ftp://data.pdbj.org/pub/pdb/validation_reports/gy/1gy7 | HTTPS FTP |
-Related structure data
Related structure data | 1gy5C 1gy6C 1gybC 1ounS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14512.100 Da / Num. of mol.: 4 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P33331 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.55 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 100MM AMMONIUM ACETATE, PH 6.5 1.12M AMMONIUM SULPHATE(WELL),1.6M AMMONIUM SULPHATE(DROP) 7 MG/ML PROTEIN - SEE PAPER | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.933 |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.933 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→46 Å / Num. obs: 66296 / % possible obs: 94 % / Redundancy: 3.4 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 5.2 |
Reflection shell | Resolution: 1.6→1.69 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.321 / Mean I/σ(I) obs: 2 / % possible all: 69.5 |
Reflection | *PLUS Lowest resolution: 46 Å / % possible obs: 94 % / Num. measured all: 353243 / Rmerge(I) obs: 0.07 |
Reflection shell | *PLUS % possible obs: 69.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1OUN Resolution: 1.6→40 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.6→40 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 40 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.4 |