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Yorodumi- PDB-1peg: Structural basis for the product specificity of histone lysine me... -
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-Basic information
Entry | Database: PDB / ID: 1peg | ||||||
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Title | Structural basis for the product specificity of histone lysine methyltransferases | ||||||
Components |
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Keywords | TRANSFERASE / Ternary structure of DIM-5 / a SUV39-type histone-H3 Lys-9 methyltransferase / SET domain protein forms a knot-like substructure / pre-SET triangular Zn3Cys9 zinc cluster / post-SET zinc-binding site / a hybrid beta sheet formed by DIM-5 and H3 tail | ||||||
Function / homology | Function and homology information SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Oxidative Stress Induced Senescence / chromatin organization => GO:0006325 / [histone H3]-lysine9 N-trimethyltransferase / Assembly of the ORC complex at the origin of replication / sexual sporulation resulting in formation of a cellular spore / histone H3K9 trimethyltransferase activity / Condensation of Prophase Chromosomes / global genome nucleotide-excision repair ...SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Oxidative Stress Induced Senescence / chromatin organization => GO:0006325 / [histone H3]-lysine9 N-trimethyltransferase / Assembly of the ORC complex at the origin of replication / sexual sporulation resulting in formation of a cellular spore / histone H3K9 trimethyltransferase activity / Condensation of Prophase Chromosomes / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / replication fork protection complex / RNA Polymerase I Promoter Escape / Estrogen-dependent gene expression / positive regulation of transcription by RNA polymerase I / histone methyltransferase activity / nucleolar large rRNA transcription by RNA polymerase I / rRNA transcription / Chromatin modifying enzymes / CENP-A containing nucleosome / epigenetic regulation of gene expression / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication / DNA methylation / Condensation of Prophase Chromosomes / HCMV Late Events / Chromatin modifications during the maternal to zygotic transition (MZT) / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / SIRT1 negatively regulates rRNA expression / PRC2 methylates histones and DNA / Defective pyroptosis / HDACs deacetylate histones / RNA Polymerase I Promoter Escape / Transcriptional regulation by small RNAs / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression / HDMs demethylate histones / PKMTs methylate histone lysines / RMTs methylate histone arginines / Meiotic recombination / Pre-NOTCH Transcription and Translation / nucleosome assembly / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / Transcriptional regulation of granulopoiesis / structural constituent of chromatin / nucleosome / gene expression / chromosome / RUNX1 regulates transcription of genes involved in differentiation of HSCs / chromatin organization / methylation / Factors involved in megakaryocyte development and platelet production / HATs acetylate histones / Senescence-Associated Secretory Phenotype (SASP) / double-stranded DNA binding / Oxidative Stress Induced Senescence / Estrogen-dependent gene expression / cadherin binding / Amyloid fiber formation / protein heterodimerization activity / regulation of DNA-templated transcription / protein-containing complex / DNA binding / extracellular exosome / zinc ion binding / extracellular region / nucleoplasm / membrane / nucleus Similarity search - Function | ||||||
Biological species | Neurospora crassa (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.59 Å | ||||||
Authors | Zhang, X. / Yang, Z. / Khan, S.I. / Horton, J.R. / Tamaru, H. / Selker, E.U. / Cheng, X. | ||||||
Citation | Journal: Mol.Cell / Year: 2003 Title: Structural basis for the product specificity of histone lysine methyltransferases Authors: Zhang, X. / Yang, Z. / Khan, S.I. / Horton, J.R. / Tamaru, H. / Selker, E.U. / Cheng, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1peg.cif.gz | 113.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1peg.ent.gz | 81.4 KB | Display | PDB format |
PDBx/mmJSON format | 1peg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pe/1peg ftp://data.pdbj.org/pub/pdb/validation_reports/pe/1peg | HTTPS FTP |
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-Related structure data
Related structure data | 1ml9S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 34111.551 Da / Num. of mol.: 2 / Fragment: residues 17-318 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neurospora crassa (fungus) / Plasmid: pXC379 / Production host: Escherichia coli (E. coli) Strain (production host): BL21(DE3) Codon plus RIL (Stratagene) Keywords: wild-type DIM-5 References: UniProt: Q8X225, histone-lysine N-methyltransferase #2: Protein/peptide | Mass: 1565.797 Da / Num. of mol.: 2 / Fragment: residues 1-15 / Source method: obtained synthetically Details: The histone H3 peptide (N-terminal residues 1-15) is synthesized. References: UniProt: P02303, UniProt: P68431*PLUS #3: Chemical | ChemComp-ZN / #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.36 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 289 K / pH: 8.4 Details: PEG 2000 monomethyl ether, trimethylamine, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K, pH 8.40 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / pH: 9.8 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 22, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.59→35 Å / Num. obs: 21803 / % possible obs: 91.6 % / Observed criterion σ(I): -3 / Redundancy: 4.5 % / Biso Wilson estimate: 59.6 Å2 / Rmerge(I) obs: 0.088 / Rsym value: 0.088 / Net I/σ(I): 23.3 |
Reflection shell | Resolution: 2.59→2.68 Å / Rmerge(I) obs: 0.228 / Mean I/σ(I) obs: 4.5 / Rsym value: 0.228 / % possible all: 67.6 |
Reflection | *PLUS Highest resolution: 2.59 Å / Lowest resolution: 35 Å |
Reflection shell | *PLUS % possible obs: 67.6 % / Num. unique obs: 1578 / Mean I/σ(I) obs: 4.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1ML9 Resolution: 2.59→30.9 Å / Rfactor Rfree error: 0.012 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 Details: THE NON-CRYSTALLOGRAPHIC SYMMETRY RESTRAINS WERE IMPOSED ON THE TWO COMPLEXES DURING THE REFINEMENT, EXCEPT THE POST-SET REGION RESIDUES 53 TO 100. MOLECULE B IS VERY FLEXIBLE IN THIS REGION ...Details: THE NON-CRYSTALLOGRAPHIC SYMMETRY RESTRAINS WERE IMPOSED ON THE TWO COMPLEXES DURING THE REFINEMENT, EXCEPT THE POST-SET REGION RESIDUES 53 TO 100. MOLECULE B IS VERY FLEXIBLE IN THIS REGION (ONLY THE CA ATOMS ARE GIVEN FOR THE RESIDUES 53 TO 87), WHILE THE CORRESPONDING REGION IN MOLECULE A IS ORDERED.
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Displacement parameters | Biso mean: 58.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.59→30.9 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.59→2.74 Å / Rfactor Rfree error: 0.064 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM / Topol file: TOPHCSDX.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 35 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Lowest resolution: 2.68 Å / Rfactor Rwork: 0.3 |