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Yorodumi- PDB-1dv9: STRUCTURAL CHANGES ACCOMPANYING PH-INDUCED DISSOCIATION OF THE B-... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dv9 | ||||||
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Title | STRUCTURAL CHANGES ACCOMPANYING PH-INDUCED DISSOCIATION OF THE B-LACTOGLOBULIN DIMER | ||||||
Components | BETA-LACTOGLOBULIN | ||||||
Keywords | TRANSPORT PROTEIN / Beta-lactoglobulin / beta-barrel / low pH structure / triple resonance experiments | ||||||
Function / homology | Function and homology information retinol binding / long-chain fatty acid binding / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Uhrinova, S. / Smith, M.H. / Jameson, G.B. / Uhrin, D. / Sawyer, L. / Barlow, P.N. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Structural changes accompanying pH-induced dissociation of the beta-lactoglobulin dimer. Authors: Uhrinova, S. / Smith, M.H. / Jameson, G.B. / Uhrin, D. / Sawyer, L. / Barlow, P.N. #1: Journal: Protein Expr.Purif. / Year: 1998 Title: Isotopically Labelled Bovine Beta-lactoglobulin Expressed in P. pastoris Authors: Denton, H. / Smith, M. / Husi, H. / Uhrin, D. / Barlow, P.N. / Batt, C.A. / Sawyer, L. #2: Journal: J.Biomol.NMR / Year: 1998 Title: Complete Assignment of 1H, 13C and 15N Chemical Shifts for Bovine Beta-lactoglobulin: Secondary Structure and Topology of the Native State is Retained in a Partially Unfolded Form. Authors: Uhrinova, S. / Uhrin, D. / Denton, H. / Smith, M. / Sawyer, L. / Barlow, P.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dv9.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1dv9.ent.gz | 910.4 KB | Display | PDB format |
PDBx/mmJSON format | 1dv9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1dv9_validation.pdf.gz | 343.6 KB | Display | wwPDB validaton report |
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Full document | 1dv9_full_validation.pdf.gz | 556.3 KB | Display | |
Data in XML | 1dv9_validation.xml.gz | 74.2 KB | Display | |
Data in CIF | 1dv9_validation.cif.gz | 92.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dv/1dv9 ftp://data.pdbj.org/pub/pdb/validation_reports/dv/1dv9 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18395.199 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: SMALL PROTEIN FOUND IN THE WHEY OF MILK OF RUMINANTS AND OTHER SPECIES Source: (gene. exp.) Bos taurus (cattle) Description: P.PASTORIS GS115/HIS+BLG/MUT+ CONTAINING PPIC9; PPIC9 DERIVED FROM PTTQ18BLG BY PCR Variant: A / Plasmid: PPIC9 / Production host: Pichia pastoris (fungus) / References: UniProt: P02754 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple resonance NMR spectra |
-Sample preparation
Details | Contents: 1mM B-lactoglobuline-15N,13C; 50mM phosphate buffer; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: not known / pH: 2.7 / Pressure: ambient / Temperature: 310 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 2715 restraints, out of which 77 are hydrogen bonds and 110 are torsion angles | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: lowest total energy / Conformers calculated total number: 60 / Conformers submitted total number: 21 |