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Open data
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Basic information
Entry | Database: PDB / ID: 1b0o | ||||||
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Title | BOVINE BETA-LACTOGLOBULIN COMPLEXED WITH PALMITATE, LATTICE Z | ||||||
![]() | BETA-LACTOGLOBULIN | ||||||
![]() | LIPOCALIN / MILK WHEY PROTEIN / BOVINE / PALMITATE-BINDING | ||||||
Function / homology | ![]() retinol binding / long-chain fatty acid binding / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wu, S.-Y. / Sawyer, L. | ||||||
![]() | ![]() Title: beta-lactoglobulin binds palmitate within its central cavity. Authors: Wu, S.Y. / Perez, M.D. / Puyol, P. / Sawyer, L. #1: ![]() Title: Structural Basis of the Tanford Transition of Bovine Beta-Lactoglobulin Authors: Qin, B.Y. / Bewley, M.C. / Creamer, L.K. / Baker, H.M. / Baker, E.N. / Jameson, G.B. #2: ![]() Title: Bovine Beta-Lactoglobulin at 1.8 A Resolution--Still an Enigmatic Lipocalin Authors: Brownlow, S. / Morais Cabral, J.H. / Cooper, R. / Flower, D.R. / Yewdall, S.J. / Polikarpov, I. / North, A.C. / Sawyer, L. #3: ![]() Title: 12-Bromododecanoic Acid Binds Inside the Calyx of Bovine Beta-Lactoglobulin Authors: Qin, B.Y. / Creamer, L.K. / Baker, E.N. / Jameson, G.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44 KB | Display | ![]() |
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PDB format | ![]() | 33.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 404.8 KB | Display | ![]() |
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Full document | ![]() | 416.4 KB | Display | |
Data in XML | ![]() | 6.5 KB | Display | |
Data in CIF | ![]() | 9.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1bebS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18301.174 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: PROTEIN PURCHASED FROM SIGMA CHEMICALS, LOT 13H7150 Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-PLM / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 50.78 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 21, 1997 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→20 Å / Num. obs: 6888 / % possible obs: 99.9 % / Redundancy: 14.1 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 21.2 |
Reflection shell | Resolution: 2.5→2.54 Å / Redundancy: 8.16 % / Rmerge(I) obs: 0.366 / Mean I/σ(I) obs: 3.95 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 97142 |
Reflection shell | *PLUS % possible obs: 100 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1BEB Resolution: 2.5→10 Å / Num. parameters: 5583 / Num. restraintsaints: 5396 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 1395 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 47.17 Å2 |