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Yorodumi- PDB-2blg: STRUCTURAL BASIS OF THE TANFORD TRANSITION OF BOVINE BETA-LACTOGL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2blg | ||||||
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Title | STRUCTURAL BASIS OF THE TANFORD TRANSITION OF BOVINE BETA-LACTOGLOBULIN FROM CRYSTAL STRUCTURES AT THREE PH VALUES; PH 8.2 | ||||||
Components | BETA-LACTOGLOBULIN | ||||||
Keywords | TRANSPORT / BETA-LACTOGLOBULIN / PH-DEPENDENT CONFORMATION / LOOP MOVEMENT / TANFORD TRANSITION | ||||||
Function / homology | Function and homology information retinol binding / long-chain fatty acid binding / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.46 Å | ||||||
Authors | Qin, B.Y. / Bewley, M.C. / Creamer, L.K. / Baker, H.M. / Baker, E.N. / Jameson, G.B. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: Structural basis of the Tanford transition of bovine beta-lactoglobulin. Authors: Qin, B.Y. / Bewley, M.C. / Creamer, L.K. / Baker, H.M. / Baker, E.N. / Jameson, G.B. #1: Journal: Thesis, Massey University / Year: 1998 Title: Structure Determination of Bovine Beta-Lactoglobulin Variants a and B Authors: Qin, B.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2blg.cif.gz | 42.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2blg.ent.gz | 32.8 KB | Display | PDB format |
PDBx/mmJSON format | 2blg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2blg_validation.pdf.gz | 420.1 KB | Display | wwPDB validaton report |
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Full document | 2blg_full_validation.pdf.gz | 422.7 KB | Display | |
Data in XML | 2blg_validation.xml.gz | 9.4 KB | Display | |
Data in CIF | 2blg_validation.cif.gz | 12.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bl/2blg ftp://data.pdbj.org/pub/pdb/validation_reports/bl/2blg | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18387.264 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Secretion: MILK / Variant: VARIANT A / References: UniProt: P02754 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 35 % / Description: DATA WERE COLLECTED USING OSCILLATION METHOD | ||||||||||||||||||||
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Crystal grow | pH: 8.2 / Details: pH 8.2 | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Oct 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.46→15 Å / Num. obs: 7203 / % possible obs: 97.1 % / Observed criterion σ(I): 2 / Redundancy: 2.34 % / Biso Wilson estimate: 52.2 Å2 / Rmerge(I) obs: 0.08 / Rsym value: 0.08 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 2.46→2.51 Å / Redundancy: 2.34 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 2 / Rsym value: 0.39 / % possible all: 89.6 |
Reflection shell | *PLUS % possible obs: 89.6 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.46→15 Å / Rfactor Rfree error: 0.011 / Data cutoff high absF: 100000 / Data cutoff low absF: 0.01 / Cross valid method: A POSTERIORI / σ(F): 0
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Displacement parameters | Biso mean: 51.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.46→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.46→2.57 Å / Rfactor Rfree error: 0.067 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.404 |