+Open data
-Basic information
Entry | Database: PDB / ID: 1bz7 | ||||||
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Title | FAB FRAGMENT FROM MURINE ASCITES | ||||||
Components |
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Keywords | IMMUNE SYSTEM / ANTIBODY (FAB FRAGMENT) | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Kaminski, M.J. / Mackenzie, C.R. / Mooibroek, M.J. / Dahms, T.E.S. / Hirama, T. / Houghton, A.N. / Chapman, P.B. / Evans, S.V. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. Authors: Kaminski, M.J. / MacKenzie, C.R. / Mooibroek, M.J. / Dahms, T.E. / Hirama, T. / Houghton, A.N. / Chapman, P.B. / Evans, S.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bz7.cif.gz | 91 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bz7.ent.gz | 70.5 KB | Display | PDB format |
PDBx/mmJSON format | 1bz7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bz7_validation.pdf.gz | 426.2 KB | Display | wwPDB validaton report |
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Full document | 1bz7_full_validation.pdf.gz | 449.6 KB | Display | |
Data in XML | 1bz7_validation.xml.gz | 19.1 KB | Display | |
Data in CIF | 1bz7_validation.cif.gz | 25.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bz/1bz7 ftp://data.pdbj.org/pub/pdb/validation_reports/bz/1bz7 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 22715.277 Da / Num. of mol.: 1 / Fragment: FAB / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
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#2: Antibody | Mass: 23018.723 Da / Num. of mol.: 1 / Fragment: FAB / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.14 Å3/Da / Density % sol: 60.79 % | ||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: used to seeding | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→6 Å / Num. obs: 18143 / % possible obs: 94 % / Observed criterion σ(I): 3 / Redundancy: 4.4 % / Rsym value: 0.05 |
Reflection | *PLUS Num. measured all: 79380 / Rmerge(I) obs: 0.05 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→6 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.5→6 Å
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Refine LS restraints |
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Xplor file | Serial no: 1 / Param file: PARAM19X.PRO / Topol file: TOPH19X.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 6 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |