+Open data
-Basic information
Entry | Database: PDB / ID: 1r24 | ||||||
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Title | FAB FROM MURINE IGG3 KAPPA | ||||||
Components |
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Keywords | IMMUNE SYSTEM / PRELIMINARY | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Evans, S.V. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. Authors: Kaminski, M.J. / MacKenzie, C.R. / Mooibroek, M.J. / Dahms, T.E. / Hirama, T. / Houghton, A.N. / Chapman, P.B. / Evans, S.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1r24.cif.gz | 156.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1r24.ent.gz | 116.2 KB | Display | PDB format |
PDBx/mmJSON format | 1r24.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1r24_validation.pdf.gz | 450.8 KB | Display | wwPDB validaton report |
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Full document | 1r24_full_validation.pdf.gz | 654 KB | Display | |
Data in XML | 1r24_validation.xml.gz | 56 KB | Display | |
Data in CIF | 1r24_validation.cif.gz | 72.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r2/1r24 ftp://data.pdbj.org/pub/pdb/validation_reports/r2/1r24 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Antibody | Mass: 22827.256 Da / Num. of mol.: 2 / Fragment: FAB / Source method: isolated from a natural source / Details: FROM ASCITES / Source: (natural) Mus musculus (house mouse) #2: Antibody | Mass: 23068.889 Da / Num. of mol.: 2 / Fragment: FAB / Source method: isolated from a natural source / Details: FROM ASCITES / Source: (natural) Mus musculus (house mouse) |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.57 Å3/Da / Density % sol: 73.06 % | ||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 270 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→6 Å / Num. obs: 26595 / % possible obs: 92 % / Observed criterion σ(I): 0 / Redundancy: 2.2 % / Biso Wilson estimate: 35.3 Å2 / Rsym value: 0.075 |
Reflection | *PLUS Num. measured all: 58762 / Rmerge(I) obs: 0.075 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: FAB FROM YST9.1 Resolution: 3.1→6 Å / σ(F): 0
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Displacement parameters | Biso mean: 28.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→6 Å
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Refine LS restraints |
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Xplor file | Serial no: 1 / Param file: PARAM19X.PRO / Topol file: TOPH19X.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3.1 Å / Lowest resolution: 6 Å / σ(F): 0 / Rfactor Rfree: 0.334 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 28.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 3.8 |