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Open data
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Basic information
Entry | Database: PDB / ID: 1aro | ||||||
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Title | T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME | ||||||
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![]() | COMPLEX (POLYMERASE/HYDROLASE) / TRANSCRIPTION / DNA-DIRECTED RNA POLYMERASE / HYDROLASE / GLYCOSIDASE / COMPLEX (POLYMERASE-HYDROLASE) / COMPLEX (POLYMERASE-HYDROLASE) complex | ||||||
Function / homology | ![]() DNA-templated viral transcription / N-acetylmuramoyl-L-alanine amidase / mitochondrial promoter sequence-specific DNA binding / N-acetylmuramoyl-L-alanine amidase activity / negative regulation of viral transcription / DNA-directed RNA polymerase complex / viral release from host cell by cytolysis / peptidoglycan catabolic process / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase ...DNA-templated viral transcription / N-acetylmuramoyl-L-alanine amidase / mitochondrial promoter sequence-specific DNA binding / N-acetylmuramoyl-L-alanine amidase activity / negative regulation of viral transcription / DNA-directed RNA polymerase complex / viral release from host cell by cytolysis / peptidoglycan catabolic process / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / host cell cytoplasm / defense response to bacterium / DNA-templated transcription / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Steitz, T. / Jeruzalmi, D. | ||||||
![]() | ![]() Title: Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. Authors: Jeruzalmi, D. / Steitz, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 198.9 KB | Display | ![]() |
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PDB format | ![]() | 154.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 98936.039 Da / Num. of mol.: 1 / Mutation: C347S, C723S, C839S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 17007.309 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P00806, N-acetylmuramoyl-L-alanine amidase |
#3: Chemical | ChemComp-HG / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.51 Å3/Da / Density % sol: 69 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 12 ℃ / Method: vapor diffusion, sitting drop / Details: Jeruzalmi, D., (1997) J. Mol. Biol., 274, 748. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Oct 1, 1993 / Details: MIRROR |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.908 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→100 Å / Num. obs: 38310 / % possible obs: 93.9 % / Observed criterion σ(I): 2 / Redundancy: 2.2 % / Rmerge(I) obs: 0.096 / Rsym value: 0.096 / Net I/σ(I): 13.37 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 2 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 2 / Rsym value: 0.44 / % possible all: 76.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refine LS restraints | *PLUS
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