+Open data
-Basic information
Entry | Database: PDB / ID: 1aif | ||||||
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Title | ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE | ||||||
Components |
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Keywords | IMMUNOGLOBULIN / C REGION / V REGION | ||||||
Function / homology | Function and homology information IgG immunoglobulin complex / B cell differentiation / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.9 Å | ||||||
Authors | Ban, N. / Escobar, C. / Hasel, K. / Day, J. / Greenwood, A. / McPherson, A. | ||||||
Citation | Journal: FASEB J. / Year: 1995 Title: Structure of an anti-idiotypic Fab against feline peritonitis virus-neutralizing antibody and a comparison with the complexed Fab. Authors: Ban, N. / Escobar, C. / Hasel, K.W. / Day, J. / Greenwood, A. / McPherson, A. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: Crystal Structure of an Idiotype-Anti-Idiotype Fab Complex Authors: Ban, N. / Escobar, C. / Garcia, R. / Hasel, K. / Day, J. / Greenwood, A. / McPherson, A. #2: Journal: J.Mol.Biol. / Year: 1993 Title: Preliminary Crystallographic Study of a Complex between an Fab of a Monoclonal Feline Peritonitis Virus Neutralizing Antibody and its Anti-Idiotypic Fab Authors: Ban, N. / Escobar, C. / Day, J. / Greenwood, A. / McPherson, A. #3: Journal: J.Mol.Biol. / Year: 1991 Title: Characterization of Crystals of an Fab Fragment of a Murine Monoclonal Antibody Authors: Ban, N. / Escobar, C. / Day, J. / Greenwood, A. / Larson, S. / McPherson, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aif.cif.gz | 168.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aif.ent.gz | 140.8 KB | Display | PDB format |
PDBx/mmJSON format | 1aif.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ai/1aif ftp://data.pdbj.org/pub/pdb/validation_reports/ai/1aif | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23560.805 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P01837 #2: Antibody | Mass: 23812.816 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: GenBank: 1042226 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.57 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 4 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Observed criterion σ(I): 3 |
Reflection | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 20 Å / Num. obs: 18603 / % possible obs: 94 % / Num. measured all: 74745 |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.9→20 Å / σ(F): 3 /
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Refinement step | Cycle: LAST / Resolution: 2.9→20 Å
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Refine LS restraints |
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Refinement | *PLUS Num. reflection obs: 16482 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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