1AIF

ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE

Summary for 1AIF

• Entry DOI: 10.2210/pdb1aif/pdb
• Descriptor: ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB (LIGHT CHAIN), ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB (HEAVY CHAIN) (2 entities in total)
• Functional Keywords: immunoglobulin, c region, v region
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 94747.24

Authors

Ban, N.,Escobar, C.,Hasel, K.,Day, J.,Greenwood, A.,McPherson, A. (deposition date: 1994-11-14, release date: 1997-02-01, Last modification date: 2024-10-30)

Primary citation

Ban, N.,Escobar, C.,Hasel, K.W.,Day, J.,Greenwood, A.,McPherson, A.
Structure of an anti-idiotypic Fab against feline peritonitis virus-neutralizing antibody and a comparison with the complexed Fab.
FASEB J., 9:107-114, 1995

PubMed Abstract: The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free from, at 2.9 A resolution by molecular replacement. This antibody, used as an immmunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained-restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 A. The free antiidiotypic Fab shows, when compared to its complexed form, a 5 degrees rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.

PubMed: 7821749

Experimental method

X-RAY DIFFRACTION (2.9 Å)