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Yorodumi- PDB-1a7o: FV FRAGMENT OF MOUSE MONOCLONAL ANTIBODY D1.3 (BALB/C, IGG1, K) R... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a7o | ||||||
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Title | FV FRAGMENT OF MOUSE MONOCLONAL ANTIBODY D1.3 (BALB/C, IGG1, K) R96L DELETION MUTANT ON VARIANT FOR CHAIN L GLU81->ASP AND CHAIN H LEU312->VAL | ||||||
Components |
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Keywords | IMMUNOGLOBULIN / DELETION MUTANT | ||||||
Function / homology | Function and homology information immunoglobulin complex / immunoglobulin mediated immune response / antigen binding / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Marks, C. / Henrick, K. / Winter, G. | ||||||
Citation | Journal: To be Published Title: X-Ray Structures of D1.3 Fv Mutants Authors: Marks, C. / Henrick, K. / Winter, G. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: Bound Water Molecules and Conformational Stabilization Help Mediate an Antigen-Antibody Association Authors: Bhat, T.N. / Bentley, G.A. / Boulot, G. / Greene, M.I. / Tello, D. / Dall'Acqua, W. / Souchon, H. / Schwarz, F.P. / Mariuzza, R.A. / Poljak, R.J. #2: Journal: Nature / Year: 1990 Title: Small Rearrangements in Structures of Fv and Fab Fragments of Antibody D1.3 On Antigen Binding Authors: Bhat, T.N. / Bentley, G.A. / Fischmann, T.O. / Boulot, G. / Poljak, R.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a7o.cif.gz | 55.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a7o.ent.gz | 42 KB | Display | PDB format |
PDBx/mmJSON format | 1a7o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a7/1a7o ftp://data.pdbj.org/pub/pdb/validation_reports/a7/1a7o | HTTPS FTP |
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-Related structure data
Related structure data | 1a7nC 1a7pC 1a7qC 1a7rC 1vfaS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 11529.757 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Mutation: DEL(R96), E81D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Variant: CHAIN L, E81D, CHAIN H, L312V / Production host: Escherichia coli (E. coli) / References: UniProt: P01635 |
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#2: Antibody | Mass: 12843.249 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Mutation: L312V Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Variant: CHAIN L, E81D, CHAIN H, L312V / Production host: Escherichia coli (E. coli) / References: UniProt: P01820 |
#3: Water | ChemComp-HOH / |
Compound details | THE ANTIBODY IS SECRETED INTO PERIPLASMIC SPACE. VH AND VL DOMAINS ARE COVALENTLY LINKED AND THEY ...THE ANTIBODY IS SECRETED INTO PERIPLASMI |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.2 % |
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-Data collection
Diffraction | Mean temperature: 269 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 1, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→17.23 Å / Num. obs: 15419 / % possible obs: 95.1 % / Observed criterion σ(I): 0 / Redundancy: 7 % / Biso Wilson estimate: 27.64 Å2 / Rmerge(I) obs: 0.084 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.329 / % possible all: 86.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1VFA Resolution: 2→6 Å / σ(F): 0 /
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Displacement parameters | Biso mean: 26.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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