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基本情報
登録情報 | データベース: PDB / ID: 1a02 | ||||||
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タイトル | STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA | ||||||
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![]() | TRANSCRIPTION/DNA / TRANSCRIPTION FACTOR / NFAT / NF-AT / AP-1 / FOS-JUN / QUATERNARY PROTEIN-DNA COMPLEX / TRANSCRIPTION SYNERGY / COMBINATORIAL GENE REGULATION / TRANSCRIPTION-DNA COMPLEX | ||||||
機能・相同性 | ![]() : / cAMP response element binding / transcription factor AP-1 complex / negative regulation of vascular associated smooth muscle cell differentiation / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / myotube cell development / mononuclear cell differentiation / cellular response to prolactin ...: / cAMP response element binding / transcription factor AP-1 complex / negative regulation of vascular associated smooth muscle cell differentiation / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / myotube cell development / mononuclear cell differentiation / cellular response to prolactin / response to gravity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / calcineurin-NFAT signaling cascade / skeletal muscle cell proliferation / cellular response to zinc ion starvation / cellular response to phorbol 13-acetate 12-myristate / WNT5:FZD7-mediated leishmania damping / conditioned taste aversion / SMAD protein signal transduction / NGF-stimulated transcription / positive regulation of osteoclast differentiation / positive regulation by host of viral transcription / cartilage development / cellular response to parathyroid hormone stimulus / myoblast proliferation / CLEC7A (Dectin-1) induces NFAT activation / nuclear chromosome / response to corticosterone / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / positive regulation of myoblast fusion / Activation of the AP-1 family of transcription factors / negative regulation of DNA binding / ubiquitin-like protein ligase binding / skeletal muscle cell differentiation / R-SMAD binding / Calcineurin activates NFAT / general transcription initiation factor binding / response to immobilization stress / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / negative regulation by host of viral transcription / response to light stimulus / phosphatase binding / RNA polymerase II core promoter sequence-specific DNA binding / positive regulation of B cell proliferation / cellular response to epidermal growth factor stimulus / positive regulation of vascular associated smooth muscle cell proliferation / 14-3-3 protein binding / response to cAMP / cellular response to cadmium ion / cellular response to calcium ion / response to muscle stretch / NPAS4 regulates expression of target genes / response to endoplasmic reticulum stress / FCERI mediated Ca+2 mobilization / GTPase activator activity / transforming growth factor beta receptor signaling pathway / osteoclast differentiation / Regulation of PTEN gene transcription / response to activity / transcription coregulator binding / response to progesterone / female pregnancy / TP53 Regulates Transcription of DNA Repair Genes / protein-DNA complex / FCERI mediated MAPK activation / B cell receptor signaling pathway / MAPK6/MAPK4 signaling / euchromatin / response to insulin / response to toxic substance / Pre-NOTCH Transcription and Translation / DNA-binding transcription repressor activity, RNA polymerase II-specific / positive regulation of miRNA transcription / Activation of anterior HOX genes in hindbrain development during early embryogenesis / cellular response to reactive oxygen species / RNA polymerase II transcription regulator complex / sequence-specific double-stranded DNA binding / Signaling by ALK fusions and activated point mutants / cell migration / cellular response to tumor necrosis factor / nervous system development / regulation of cell population proliferation / cellular response to hypoxia / Senescence-Associated Secretory Phenotype (SASP) / DNA-binding transcription activator activity, RNA polymerase II-specific / Interleukin-4 and Interleukin-13 signaling / Oxidative Stress Induced Senescence / response to ethanol / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription by RNA polymerase II / response to lipopolysaccharide / molecular adaptor activity / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / ribonucleoprotein complex / response to xenobiotic stimulus / inflammatory response 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Chen, L. / Glover, J.N.M. / Hogan, P.G. / Rao, A. / Harrison, S.C. | ||||||
![]() | ![]() タイトル: Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA. 著者: Chen, L. / Glover, J.N. / Hogan, P.G. / Rao, A. / Harrison, S.C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 114.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 84.7 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 455.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 471.4 KB | 表示 | |
XML形式データ | ![]() | 19.4 KB | 表示 | |
CIF形式データ | ![]() | 26.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
-DNA鎖 , 2種, 2分子 AB
#1: DNA鎖 | 分子量: 6178.024 Da / 分子数: 1 / 由来タイプ: 合成 |
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#2: DNA鎖 | 分子量: 6084.993 Da / 分子数: 1 / 由来タイプ: 合成 |
-AP-1 FRAGMENT ... , 2種, 2分子 FJ
#4: タンパク質 | 分子量: 6727.733 Da / 分子数: 1 / Fragment: FOS / Mutation: C154S / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#5: タンパク質 | 分子量: 6583.826 Da / 分子数: 1 / Fragment: JUN / Mutation: C279S / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
-タンパク質 / 非ポリマー , 2種, 89分子 N![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#3: タンパク質 | 分子量: 34270.898 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#6: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.54 Å3/Da / 溶媒含有率: 68 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: THE COMPLEX WAS CRYSTALLIZED IN 300-400 MM AMMONIUM ACETATE SALT, PH 7.5 (10 MM)., VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 |
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結晶 | *PLUS 溶媒含有率: 68 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1996年9月16日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 相対比: 1 |
反射 | 解像度: 2.7→20 Å / Num. obs: 22079 / % possible obs: 98.3 % / Observed criterion σ(I): 0 / 冗長度: 3.1 % / Biso Wilson estimate: 61 Å2 / Rsym value: 0.08 |
反射 シェル | 解像度: 2.7→2.8 Å / 冗長度: 2.7 % / Rsym value: 0.43 / % possible all: 93.3 |
反射 | *PLUS 最高解像度: 2.7 Å / 最低解像度: 20 Å / % possible obs: 98.3 % / 冗長度: 3.1 % / Rmerge(I) obs: 0.08 / Biso Wilson estimate: 61 Å2 |
反射 シェル | *PLUS 最高解像度: 2.7 Å / 最低解像度: 2.8 Å / % possible obs: 93.3 % / Rmerge(I) obs: 0.43 / Mean I/σ(I) obs: 2.6 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: MIR/MAD / 解像度: 2.7→10 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.1 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 2 詳細: RESIDUES N 478 - N 485 AND N 628 - N 634 ARE DISORDERED
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原子変位パラメータ | Biso mean: 51 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati d res low obs: 10 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.7→10 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.7→2.82 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 8
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Xplor file |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.7 Å / 最低解像度: 10 Å / σ(F): 2 / % reflection Rfree: 7.5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor obs: 0.369 |