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Yorodumi- PDB-6kfm: Amino-transferase (AMT) Domain of Hybrid Polyketide/Non-Ribosomal... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6kfm | ||||||
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Title | Amino-transferase (AMT) Domain of Hybrid Polyketide/Non-Ribosomal Peptide Synthetase | ||||||
Components | Mycosubtilin synthase subunit A | ||||||
Keywords | TRANSFERASE / Amino-transferase / Hybrid Polyketide/Non-Ribosomal Peptide Synthetase | ||||||
Function / homology | Function and homology information transaminase activity / phosphopantetheine binding / ligase activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / antibiotic biosynthetic process / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / fatty acid biosynthetic process / pyridoxal phosphate binding Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Tian, Q.W. / Jiang, T. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Structure Insights into the Molecular Mechanism of Two Tailoring Domains of Hybrid Polyketide/Non-Ribosomal Peptide Synthetase Authors: Tian, Q.W. / Jiang, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6kfm.cif.gz | 203.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6kfm.ent.gz | 157.7 KB | Display | PDB format |
PDBx/mmJSON format | 6kfm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kf/6kfm ftp://data.pdbj.org/pub/pdb/validation_reports/kf/6kfm | HTTPS FTP |
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-Related structure data
Related structure data | 6ketC 6kfrC 6kfuC 5hdmS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 51454.594 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: mycA / Production host: Escherichia coli BL21 (bacteria) References: UniProt: Q9R9J1, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.31 % |
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Crystal grow | Temperature: 312 K / Method: vapor diffusion, hanging drop / pH: 6.7 Details: 10 mM MgCl2, 5 mM NiCl, 0.1 M HEPES, pH 6.7, 18% (v/v) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.9789 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Oct 23, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. obs: 110351 / % possible obs: 99.9 % / Redundancy: 13 % / Biso Wilson estimate: 20.88 Å2 / CC1/2: 0.993 / Rpim(I) all: 0.03 / Rrim(I) all: 0.111 / Χ2: 0.928 / Net I/σ(I): 27.2 |
Reflection shell | Resolution: 1.7→1.73 Å / Redundancy: 11.4 % / Mean I/σ(I) obs: 3 / Num. unique obs: 5395 / CC1/2: 0.87 / Rpim(I) all: 0.231 / Rrim(I) all: 0.811 / Χ2: 0.808 / % possible all: 98.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5HDM Resolution: 1.7→45.23 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 14.73 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→45.23 Å
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LS refinement shell | Resolution: 1.7→1.761 Å
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