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- EMDB-8863: Negative-stain reconstruction of the S. pombe CTP complex (Ccq1 2... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-8863 | |||||||||
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Title | Negative-stain reconstruction of the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) | |||||||||
![]() | Negative-stain reconstruction of the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249). | |||||||||
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Function / homology | ![]() telomere-telomerase complex assembly / telomere cap complex / chromosome, telomeric repeat region / meiotic chromosome segregation / meiotic telomere clustering / chromosome, subtelomeric region / shelterin complex / mitotic telomere maintenance via semi-conservative replication / telomere capping / protein localization to chromosome, telomeric region ...telomere-telomerase complex assembly / telomere cap complex / chromosome, telomeric repeat region / meiotic chromosome segregation / meiotic telomere clustering / chromosome, subtelomeric region / shelterin complex / mitotic telomere maintenance via semi-conservative replication / telomere capping / protein localization to chromosome, telomeric region / telomeric DNA binding / telomere maintenance via telomerase / telomere organization / telomere maintenance / molecular adaptor activity / DNA binding / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 34.4 Å | |||||||||
![]() | Scott HW / Kim JK / Yu C / Huang L / Qiao F / Taylor DJ | |||||||||
![]() | ![]() Title: Spatial Organization and Molecular Interactions of the Schizosaccharomyces pombe Ccq1-Tpz1-Poz1 Shelterin Complex. Authors: Harry Scott / Jin-Kwang Kim / Clinton Yu / Lan Huang / Feng Qiao / Derek J Taylor / ![]() Abstract: The shelterin complex is a macromolecular assembly of proteins that binds to and protects telomeric DNA, which composes the ends of all linear chromosomes. Shelterin proteins prevent chromosome ends ...The shelterin complex is a macromolecular assembly of proteins that binds to and protects telomeric DNA, which composes the ends of all linear chromosomes. Shelterin proteins prevent chromosome ends from fusing together and from eliciting erroneous induction of DNA damage response pathways. In addition, shelterin proteins play key roles in regulating the recruitment and activation of telomerase, an enzyme that extends telomeric DNA. In fission yeast, Schizosaccharomyces pombe, interactions between the shelterin proteins Ccq1, Tpz1, and Poz1 are important for regulating telomerase-mediated telomere synthesis and thus telomere length homeostasis. Here, we used electron microscopy combined with genetic labeling to define the three-dimensional arrangement of the S. pombe Ccq1-Tpz1-Poz1 (CTP) complex. Crosslinking mass spectrometry was used to identify individual residues that are in proximity to the protein-protein interfaces of the assembled CTP complex. Together, our data provide a first glimpse into the architectural design of the CTP complex and reveals unique interactions that are important in maintaining the S. pombe telomere in a non-extendible state. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.8 KB 11.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 3.7 KB | Display | ![]() |
Images | ![]() | 16.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8864C ![]() 8865C ![]() 8866C ![]() 8861 C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Negative-stain reconstruction of the S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.66 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) as a ...
Entire | Name: S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) as a dimer of trimers |
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Components |
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-Supramolecule #1: S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) as a ...
Supramolecule | Name: S. pombe CTP complex (Ccq1 2-716, Tpz1 406-508, Poz1 2-249) as a dimer of trimers type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | negative staining |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.053 mg/mL | ||||||||||||
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Buffer | pH: 8 Component:
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Staining | Type: NEGATIVE / Material: Uranyl acetate | ||||||||||||
Grid | Pretreatment - Type: GLOW DISCHARGE / Details: 15 mA |
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Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: TVIPS TEMCAM-F416 (4k x 4k) / Digitization - Dimensions - Width: 4000 pixel / Digitization - Dimensions - Height: 4000 pixel / Digitization - Sampling interval: 15.6 µm / Average exposure time: 1.0 sec. / Average electron dose: 25.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |