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- EMDB-8476: human CTP synthase 1 - mutant H355A -

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Basic information

Database: EMDB / ID: 8476
Titlehuman CTP synthase 1 - mutant H355A
SamplehCTPS1-H355A tetramer
SourceHomo sapiens / human
Map datahuman CTP synthase 1 - mutant H355A
Methodsingle particle reconstruction, at 17 Å resolution
AuthorsLynch EM / Kollman JM
CitationNat. Struct. Mol. Biol., 2017, 24, 507-514

Nat. Struct. Mol. Biol., 2017, 24, 507-514 Yorodumi Papers
Human CTP synthase filament structure reveals the active enzyme conformation.
Eric M Lynch / Derrick R Hicks / Matthew Shepherd / James A Endrizzi / Allison Maker / Jesse M Hansen / Rachael M Barry / Zemer Gitai / Enoch P Baldwin / Justin M Kollman

DateDeposition: Nov 22, 2016 / Header (metadata) release: Feb 8, 2017 / Map release: Apr 26, 2017 / Last update: Jun 21, 2017

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.2
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by radius
  • Surface level: 0.2
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

fix: /
Orientation Rotation
Misc. /
Supplemental images

Downloads & links


Fileemd_8476.map.gz (map file in CCP4 format, 5620 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
112 pix
2.07 Å/pix.
= 231.84 Å
112 pix
2.07 Å/pix.
= 231.84 Å
112 pix
2.07 Å/pix.
= 231.84 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 2.07 Å
Contour Level:0.2 (by author), 0.2 (movie #1):
Minimum - Maximum-0.012666393 - 0.38952807
Average (Standard dev.)0.015670443 (0.05302331)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 231.84 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.072.072.07
M x/y/z112112112
origin x/y/z0.0000.0000.000
length x/y/z231.840231.840231.840
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-56-56-56
D min/max/mean-0.0130.3900.016

Supplemental data

Sample components

Entire hCTPS1-H355A tetramer

EntireName: hCTPS1-H355A tetramer / Number of components: 2

Component #1: protein, hCTPS1-H355A tetramer

ProteinName: hCTPS1-H355A tetramer / Recombinant expression: No
SourceSpecies: Homo sapiens / human
Source (engineered)Expression System: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /
Vector: pDO105-hCTPS1-H355A

Component #2: protein, hCTPS1-H355A

ProteinName: hCTPS1-H355A / Recombinant expression: No
Source (engineered)Expression System: Homo sapiens / human

Experimental details

Sample preparation

Specimen stateparticle
Sample solutionpH: 7.9
VitrificationCryogen name: NONE

Electron microscopy imaging

Experimental equipment
Model: Tecnai Spirit / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI SPIRIT
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 120 kV / Electron dose: 40 e/Å2 / Illumination mode: OTHER
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN ULTRASCAN 4000 (4k x 4k)

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: D2 (2*2 fold dihedral) / Number of projections: 4413
3D reconstructionSoftware: RELION / Resolution: 17 Å / Resolution method: FSC 0.143 CUT-OFF

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