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- EMDB-8490: E. coli CTP synthase CC mutant filament -

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Basic information

Entry
Database: EMDB / ID: 8490
TitleE. coli CTP synthase CC mutant filament
SampleE. coli CTP synthase CC mutant filament
SourceEscherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
Map dataE. coli CTP synthase CC mutant filament
Methodhelical reconstruction, at 7.6 A resolution
AuthorsLynch E / Kollman J
CitationNat. Struct. Mol. Biol., 2017

Nat. Struct. Mol. Biol., 2017 StrPapers
Human CTP synthase filament structure reveals the active enzyme conformation.
Eric M Lynch / Derrick R Hicks / Matthew Shepherd / James A Endrizzi / Allison Maker / Jesse M Hansen / Rachael M Barry / Zemer Gitai / Enoch P Baldwin / Justin M Kollman

DateDeposition: Nov 30, 2016 / Header (metadata) release: Feb 8, 2017 / Map release: Apr 26, 2017 / Last update: Nov 30, 2016

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 1.4
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 1.4
  • Imaged by UCSF CHIMERA
  • Download
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Supplemental images

Downloads & links

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Map

Fileemd_8490.map.gz (map file in CCP4 format, 131073 KB)
Projections & slices

Image control

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AxesZ (Sec.)Y (Row.)X (Col.)
320 pix
1.26 A/pix
= 403.2 A
320 pix
1.26 A/pix
= 403.2 A
320 pix
1.26 A/pix
= 403.2 A

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.26 A
Density
Contour Level:1.4 (by author), 1.4 (movie #1):
Minimum - Maximum-2.9928074 - 4.3349466
Average (Standard dev.)-3.404079E-9 (0.27666792)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions320320320
Origin-160-160-160
Limit159159159
Spacing320320320
CellA=B=C: 403.2 A
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z1.261.261.26
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z403.200403.200403.200
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ370370370
MAP C/R/S123
start NC/NR/NS-160-160-160
NC/NR/NS320320320
D min/max/mean-2.9934.335-0.000

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Supplemental data

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Sample components

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Entire E. coli CTP synthase CC mutant filament

EntireName: E. coli CTP synthase CC mutant filament
Details: Disulfide-crosslinked E. coli CTP synthase filaments assembled in non-reducing buffer
Number of components: 1

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Component #1: protein, E. coli CTP synthase CC mutant filament

ProteinName: E. coli CTP synthase CC mutant filament
Details: Disulfide-crosslinked E. coli CTP synthase filaments assembled in non-reducing buffer
Recombinant expression: No
SourceSpecies: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
Source (engineered)Expression System: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
Vector: pET22b

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Experimental details

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Sample preparation

Specimen statefilament
Helical parametersAxial symmetry: C2 (2 fold cyclic) / Delta z: 82.3 A / Delta phi: 48.2 deg.
Sample solutionSpecimen conc.: 0.15 mg/ml / pH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 45 e/A2 / Illumination mode: OTHER
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 (4k x 4k)

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Image processing

ProcessingMethod: helical reconstruction
3D reconstructionSoftware: SPIDER, hsearch_lorentz / Resolution: 7.6 A / Resolution method: FSC 0.143 CUT-OFF

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