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- PDB-5u05: Cryo-EM structure of the E. coli CTP synthase tetramer -

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Database: PDB / ID: 5u05
TitleCryo-EM structure of the E. coli CTP synthase tetramer
DescriptorCTP synthase (E.C.
KeywordsLIGASE / nucleotide metabolism / enzyme / active
Specimen sourceEscherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
MethodElectron microscopy (7.9 A resolution / Single particle)
AuthorsLynch, E.M. / Hicks, D.R. / Kollman, J.M.
CitationNat. Struct. Mol. Biol., 2017

Nat. Struct. Mol. Biol., 2017 StrPapers
Human CTP synthase filament structure reveals the active enzyme conformation.
Eric M Lynch / Derrick R Hicks / Matthew Shepherd / James A Endrizzi / Allison Maker / Jesse M Hansen / Rachael M Barry / Zemer Gitai / Enoch P Baldwin / Justin M Kollman

DateDeposition: Nov 22, 2016 / Release: Apr 26, 2017 / Last modification: May 17, 2017

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Deposited unit
A: CTP synthase
B: CTP synthase
C: CTP synthase
D: CTP synthase

Theoretical massNumber of molelcules
Total (without water)241,7884

TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (A2)6260
ΔGint (kcal/M)-39
Surface area (A2)85480


#1: Polypeptide(L)
CTP synthase / Cytidine 5'-triphosphate synthase / Cytidine triphosphate synthetase / CTPS / UTP--ammonia ligase

Mass: 60446.980 Da / Num. of mol.: 4
Source: (gene. exp.) Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
References: UniProt: B7MLA1, EC:

Experimental details


EM experimentReconstruction method: SINGLE PARTICLE

Sample preparation

ComponentName: E. coli CTP synthase tetramer / Type: COMPLEX
VitrificationCryogen name: ETHANE

Electron microscopy imaging

MicroscopyMicroscope model: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD


3D reconstructionResolution: 7.9 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 6407

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