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- EMDB-61603: Cryo-EM structure of CN-HedgehogCoV (HKU31/Erinaceus amurensis/Ch... -

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Basic information

Entry
Database: EMDB / ID: EMD-61603
TitleCryo-EM structure of CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) S-trimer in a locked-2 conformation
Map data
Sample
  • Organelle or cellular component: CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein
    • Protein or peptide: Spike glycoprotein,Fibritin
  • Ligand: FOLIC ACID
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Keywordsspike protein / VIRAL PROTEIN
Function / homology
Function and homology information


virion component / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated virion attachment to host cell / endocytosis involved in viral entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / host cell plasma membrane / virion membrane / membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, MERS-CoV-like / Spike (S) protein S1 subunit, N-terminal domain, MERS-CoV-like / Fibritin C-terminal / Fibritin C-terminal region / Spike glycoprotein S2, coronavirus, C-terminal / Coronavirus spike glycoprotein S2, intravirion / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily ...Spike (S) protein S1 subunit, receptor-binding domain, MERS-CoV-like / Spike (S) protein S1 subunit, N-terminal domain, MERS-CoV-like / Fibritin C-terminal / Fibritin C-terminal region / Spike glycoprotein S2, coronavirus, C-terminal / Coronavirus spike glycoprotein S2, intravirion / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2
Similarity search - Domain/homology
Spike glycoprotein / Fibritin
Similarity search - Component
Biological speciesErinaceus hedgehog coronavirus HKU31 / Enterobacteria phage T4 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsYuan H / Xiong X
Funding support3 items
OrganizationGrant numberCountry
Other governmentSRPG22-002
Other government201YFA1300903
Other government2022A1515110495
CitationJournal: To Be Published
Title: A locked-2 conformation of spike protein trimer of Merbecovirus
Authors: Yuan H / Xiong X
History
DepositionSep 20, 2024-
Header (metadata) releaseJun 18, 2025-
Map releaseJun 18, 2025-
UpdateJun 18, 2025-
Current statusJun 18, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_61603.png

Downloads & links

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Map

FileDownload / File: emd_61603.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 240 pix.
= 316.8 Å		1.32 Å/pix.
x 240 pix.
= 316.8 Å		1.32 Å/pix.
x 240 pix.
= 316.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.32 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.025
Minimum - Maximum-0.13516945 - 0.19227405
Average (Standard dev.)0.00012668248 (±0.0074494462)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 316.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_61603_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_61603_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein

EntireName: CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein
Components
  • Organelle or cellular component: CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein
    • Protein or peptide: Spike glycoprotein,Fibritin
  • Ligand: FOLIC ACID
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein

SupramoleculeName: CN-HedgehogCoV (HKU31/Erinaceus amurensis/China/2014) Spike protein
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Erinaceus hedgehog coronavirus HKU31

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Macromolecule #1: Spike glycoprotein,Fibritin

MacromoleculeName: Spike glycoprotein,Fibritin / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Enterobacteria phage T4 (virus)
Molecular weightTheoretical: 149.577219 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MIRSVFLLMC LLTLIKSGDA NCIVVDMQPS YFQRDWPRPI NMSAADGIIY PVGRTYANIT IMLSGLFPHQ ADKGKQYIYS MYHMKFNQG VNDPFISNYS YYTEPFDNGF VVRIGANSNI TGSSVVGSAN ATVKKIYPAL MLGSVVGKFP SNKTGRYFNH T LVILPEKC ...String:
MIRSVFLLMC LLTLIKSGDA NCIVVDMQPS YFQRDWPRPI NMSAADGIIY PVGRTYANIT IMLSGLFPHQ ADKGKQYIYS MYHMKFNQG VNDPFISNYS YYTEPFDNGF VVRIGANSNI TGSSVVGSAN ATVKKIYPAL MLGSVVGKFP SNKTGRYFNH T LVILPEKC GTQITALYCV LQPKNQTNCT GHTTYTSYVL VENKTCSTDA DGNLKQSLQT WFDLKDCLFE KKYNVSADER EE WFGITQD QQGVHLYTSR KNGFKSNNMF LFATLPIYER LRYYTVMPRS IKTTANNDHM AWSAFYIYQL HKLNYLVEFD VEG YIVRAS DCGANDYTQL QCSYGQFDMN SGVYSASYFN AQPRGYYYVA NELEECALDV LFKNIAPQIA NYSRRVFTNC NYNL TKLLS LVEVDEFVCD KTTPESLATG CYSSLVVDWF ALPLSMKSTL AIGSAEAISM FNYNQDYSNP TCRIHATINS NVSSS LNFT ANNNYAYISR CQGTDGKPIL LQKGQLPNIA CRSGVLGLSN DVDYFGYNFN GRIFYIGRKS YTPRTSEGSI QMVYVI TAN YAEGPNNVCP LKDTTTATDN LDSLLGQCID YDINGVVGQG VFQKCNSTGI PSQVFVYDSF HNIIGYHSKN GTYYCMA PC VSVPVSVIYD KSSNSHATLY GSVACDHIKM IPSVLSRETA TKLRASDNGL LQTAVGCVIG FHNTSDTVED CNLPLGQS L CAKPPSLTRS ANNTFGLAVM KYENPLKVEV LNSSEFEISV PTNFSFRVTE EFIETSIQKV TVDCKQYVCN GFERCEQLL EQYGQFCAKI NQALHGVNLR QDDATKSLFE SIRVPQSAPL MASLSGDYNL SLFETPSINT GGSGNYRSAL EDLLFDKVTL SVPGYMKGY DECMKKGPPS ARDLICAQYV AGYKVLPPLY DSNMEAMYTA SLTGSIAGAL WTGGLSSAAS LPFAQSIYYR M NGIGITQN VLMENQKLIA NKFNQALGAM QTGFTTTNQA FQKVQDAVNA NAQALSKLAS ELSNTFGAIS ASIGDILKRL DT LEQEVQI DRLINGRLTS LNAFVSQQLV RSEAAARSSQ LVKDKVNECV KSQSSRNGFC GQGTHIVSFA VNAPNGFYFV HVG YHPTDY VNQTSAYGLC DSSNTKCIAA KNGYFIKNGS DDWMFTGSSY YQPEPITNFN SRSVEPEVTF QNLTNNLPPP LSNS TDVDF KDELEEFFKN ITSEVPNFGS ITQINSTVLD LSEEMKTLQS VVEALNQSYI ELKELGNYTY YNKWPGSGYI PEAPR DGQA YVRKDGEWVL LSTFLLEVLF QGPGHHHHHH HHSAWSHPQF EKGGGSGGGG SGGSAWSHPQ FEKSA

UniProtKB: Spike glycoprotein, Fibritin

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Macromolecule #4: FOLIC ACID

MacromoleculeName: FOLIC ACID / type: ligand / ID: 4 / Number of copies: 3 / Formula: FOL
Molecular weightTheoretical: 441.397 Da
Chemical component information

ChemComp-FA:
FOLIC ACID

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Macromolecule #5: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 5 / Number of copies: 39 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6q04

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 63700
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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