EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K5...

基本情報

• 登録情報: データベース: EMDB / ID: EMD-4525
• タイトル: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• マップデータ: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map

試料

• 複合体: Human post-catalytic P complex spliceosome
  • 複合体: Human post-catalytic P complex spliceosome
    • RNA: x 3種
    • タンパク質・ペプチド: x 38種
  • 複合体: ATP-dependent RNA helicase DHX8
    • タンパク質・ペプチド: x 1種
  • 複合体: MINX introns and exons
    • RNA: x 2種
• リガンド: x 6種

• キーワード: spliceosome / RNA / complex / SPLICING

機能・相同性

分子機能:

regulation of homologous chromosome segregation / endoplasmic reticulum membrane organization / second spliceosomal transesterification activity / exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / negative regulation of nitric-oxide synthase activity / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / post-spliceosomal complex / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / pre-mRNA 3'-splice site binding / NOSIP mediated eNOS trafficking / protein exit from endoplasmic reticulum / granulocyte differentiation / negative regulation of phosphorylation / regulation of translation at postsynapse, modulating synaptic transmission / negative regulation of catalytic activity / regulation of retinoic acid receptor signaling pathway / post-mRNA release spliceosomal complex / renal system process / negative regulation of toll-like receptor signaling pathway / U2 snRNP binding / U7 snRNA binding / negative regulation of excitatory postsynaptic potential / histone pre-mRNA DCP binding / U7 snRNP / 3'-5' RNA helicase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / generation of catalytic spliceosome for first transesterification step / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / histone pre-mRNA 3'end processing complex / alternative mRNA splicing, via spliceosome / cis assembly of pre-catalytic spliceosome / regulation of vitamin D receptor signaling pathway / negative regulation of interleukin-8 production / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / negative regulation of lipopolysaccharide-mediated signaling pathway / regulation of mRNA processing / Deadenylation of mRNA / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / nuclear retinoic acid receptor binding / embryonic brain development / protein methylation / negative regulation of interferon-beta production / U12-type spliceosomal complex / poly(A) binding / 7-methylguanosine cap hypermethylation / M-decay: degradation of maternal mRNAs by maternally stored factors / U1 snRNP binding / RNA splicing, via transesterification reactions / mRNA 3'-end processing / sno(s)RNA-containing ribonucleoprotein complex / methylosome / ATP-dependent activity, acting on RNA / pICln-Sm protein complex / embryonic cranial skeleton morphogenesis / regulation of mRNA splicing, via spliceosome / oocyte development / C2H2 zinc finger domain binding / U2-type catalytic step 1 spliceosome / positive regulation of mRNA splicing, via spliceosome / pre-mRNA binding / snRNP binding / small nuclear ribonucleoprotein complex / regulation of nitric oxide biosynthetic process / telomerase holoenzyme complex / SMN-Sm protein complex / P granule / telomerase RNA binding / spliceosomal tri-snRNP complex / positive regulation by host of viral transcription / U2-type precatalytic spliceosome / U2-type spliceosomal complex / positive regulation of vitamin D receptor signaling pathway / commitment complex / mRNA cis splicing, via spliceosome / U2-type prespliceosome assembly / Transport of Mature mRNA derived from an Intron-Containing Transcript / nuclear vitamin D receptor binding / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U2-type catalytic step 2 spliceosome / RUNX3 regulates NOTCH signaling / positive regulation of alpha-beta T cell differentiation / NOTCH4 Intracellular Domain Regulates Transcription / U4 snRNP / U2 snRNP / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / exploration behavior / U1 snRNP / NOTCH3 Intracellular Domain Regulates Transcription / U2-type prespliceosome / WD40-repeat domain binding / protein peptidyl-prolyl isomerization / inner cell mass cell proliferation / regulation of alternative mRNA splicing, via spliceosome / negative regulation of type I interferon-mediated signaling pathway

ドメイン・相同性:

XAP5 protein / Nitric oxide synthase-interacting protein / STEEP-like / Nitric oxide synthase-interacting protein, zinc-finger / : / XAP5, circadian clock regulator C-terminal domain / Zinc-finger of nitric oxide synthase-interacting protein / Telomere length and silencing protein 1 / Hepatocellular carcinoma-associated antigen 59 / NF-kappa-B-activating protein, C-terminal / NF-kappa-B-activating protein / NF-kappa-B-activating protein C-terminal domain / NF-kappa-B-activating protein / Nuclear protein DGCR14/ESS-2 / : / Nuclear protein Es2 / Vertebrate Splicing regulator sde2, N-terminal ubiquitin domain / Protein FAM32A / Cactin, central domain / Cactin, C-terminal / FAM32A / Cactus-binding C-terminus of cactin protein / Conserved mid region of cactin / PRKR-interacting protein 1 / Protein of unknown function (DUF1168) / mRNA splicing factor Cwf18-like / : / cwf18 pre-mRNA splicing factor / Splicing regulator SDE2, SDE2 domain / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / : / : / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / Prp19 WD40 domain / : / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Peptidyl-prolyl cis-trans isomerase E / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / mRNA splicing factor SYF2 / SYF2 splicing factor / Cactus-binding C-terminus of cactin protein / Helix hairpin bin domain superfamily / : / Replication stress response SDE2 C-terminal / : / : / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / U-box domain / : / : / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / G10 protein signature 2. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / G10 protein signature 1. / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / DNA2/NAM7 helicase, helicase domain / : / AAA domain / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / : / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal

構成要素:

Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor SYF2 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Eukaryotic initiation factor 4A-III / Protein BUD31 homolog / Protein mago nashi homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / Protein FAM50A / ATP-dependent RNA helicase DHX8 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing regulator SDE2 / Pre-mRNA-processing-splicing factor 8 / NF-kappa-B-activating protein / Coiled-coil domain-containing protein 12 / Splicing factor Cactin / Protein mago nashi homolog 2 / Peptidylprolyl isomerase domain and WD repeat-containing protein 1 / Splicing factor ESS-2 homolog / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Crooked neck-like protein 1 / STING ER exit protein / PRKR-interacting protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / Splicing factor C9orf78 / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor ISY1 homolog / Pre-mRNA-processing factor 19 / Peptidyl-prolyl cis-trans isomerase E / Serine/arginine repetitive matrix protein 2 / Nitric oxide synthase-interacting protein / Peptidyl-prolyl cis-trans isomerase-like 1 / Protein FAM32A / RNA-binding protein 8A

• 生物種: Homo sapiens (ヒト) / Human adenovirus 2 (ヒトアデノウイルス)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å
• データ登録者: Fica SM / Oubridge C

資金援助

英国, 2件

Organization	Grant number	国
Medical Research Council (United Kingdom)	MC_U105184330	英国
European Research Council	AdG-693087-SPLICE3D

• 引用: ジャーナル: Science / 年: 2019; タイトル: A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.; 著者: Sebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai / ; 要旨: During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.

履歴

登録	2019年1月3日	-
ヘッダ(付随情報) 公開	2019年2月20日	-
マップ公開	2019年2月20日	-
更新	2024年11月13日	-
現状	2024年11月13日	処理サイト: PDBe / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_4525.map.gz / 形式: CCP4 / 大きさ: 262.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• 注釈: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• ボクセルのサイズ: X=Y=Z: 1.2 Å

密度

表面レベル	登録者による: 0.024 / ムービー #1: 0.024
最小 - 最大	-0.10098974 - 0.17720254
平均 (標準偏差)	0.000077332166 (±0.0050888886)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 410 410 410 Spacing 410 410 410
セル	A=B=C: 492.00003 Å α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.2	1.2	1.2
M x/y/z	410	410	410
origin x/y/z	0.000	0.000	0.000
length x/y/z	492.000	492.000	492.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	364	364	364
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	410	410	410
D min/max/mean	-0.101	0.177	0.000

添付データ

試料の構成要素

全体 : Human post-catalytic P complex spliceosome

• 全体: 名称: Human post-catalytic P complex spliceosome

要素

• 複合体: Human post-catalytic P complex spliceosome
  • 複合体: Human post-catalytic P complex spliceosome
    • RNA: U2 snRNA
    • RNA: U5 snRNA
    • RNA: U6 snRNA
    • タンパク質・ペプチド: Eukaryotic initiation factor 4A-III, N-terminally processed
    • タンパク質・ペプチド: RNA-binding protein 8A
    • タンパク質・ペプチド: Protein mago nashi homolog 2
    • タンパク質・ペプチド: Pre-mRNA-processing-splicing factor 8
    • タンパク質・ペプチド: U5 small nuclear ribonucleoprotein 200 kDa helicase
    • タンパク質・ペプチド: 116 kDa U5 small nuclear ribonucleoprotein component
    • タンパク質・ペプチド: PRKR-interacting protein 1
    • タンパク質・ペプチド: Cactin
    • タンパク質・ペプチド: Protein FAM32A
    • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC22 homolog
    • タンパク質・ペプチド: Pleiotropic regulator 1
    • タンパク質・ペプチド: SNW domain-containing protein 1
    • タンパク質・ペプチド: Protein BUD31 homolog
    • タンパク質・ペプチド: Pre-mRNA-splicing factor RBM22
    • タンパク質・ペプチド: U5 small nuclear ribonucleoprotein 40 kDa protein
    • タンパク質・ペプチド: Cell division cycle 5-like protein
    • タンパク質・ペプチド: Spliceosome-associated protein CWC15 homolog
    • タンパク質・ペプチド: Serine/arginine repetitive matrix protein 2
    • タンパク質・ペプチド: Crooked neck-like protein 1
    • タンパク質・ペプチド: Pre-mRNA-splicing factor SYF1
    • タンパク質・ペプチド: Intron-binding protein aquarius
    • タンパク質・ペプチド: U2 small nuclear ribonucleoprotein A'
    • タンパク質・ペプチド: U2 small nuclear ribonucleoprotein B''
    • タンパク質・ペプチド: NF-kappa-B-activating protein
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein-associated proteins B and B'
    • タンパク質・ペプチド: Pre-mRNA-splicing factor SLU7
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D3
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein E
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein F
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein G
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D1
    • タンパク質・ペプチド: Peptidyl-prolyl cis-trans isomerase-like 1
    • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D2
    • タンパク質・ペプチド: Pre-mRNA-processing factor 17
    • タンパク質・ペプチド: Pre-mRNA-splicing factor SPF27
    • タンパク質・ペプチド: Pre-mRNA-processing factor 19
    • タンパク質・ペプチド: Pre-mRNA-splicing factor SYF2
    • タンパク質・ペプチド: Replication stress response regulator SDE2
  • 複合体: ATP-dependent RNA helicase DHX8
    • タンパク質・ペプチド: ATP-dependent RNA helicase DHX8
  • 複合体: MINX introns and exons
    • RNA: Ligated exons: MINX mRNA
    • RNA: Intron lariat: MINX RNA
• リガンド: MAGNESIUM ION
• リガンド: POTASSIUM ION
• リガンド: ADENOSINE-5'-TRIPHOSPHATE
• リガンド: GUANOSINE-5'-TRIPHOSPHATE
• リガンド: ZINC ION
• リガンド: INOSITOL HEXAKISPHOSPHATE

超分子 #1: Human post-catalytic P complex spliceosome

• 超分子: 名称: Human post-catalytic P complex spliceosome / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#44
• 分子量: 理論値: 3.0 MDa

超分子 #2: Human post-catalytic P complex spliceosome

• 超分子: 名称: Human post-catalytic P complex spliceosome / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1-#10, #12-#14, #16-#26, #28-#44
• 由来(天然): 生物種: Homo sapiens (ヒト)

超分子 #3: ATP-dependent RNA helicase DHX8

• 超分子: 名称: ATP-dependent RNA helicase DHX8 / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #27
• 由来(天然): 生物種: Homo sapiens (ヒト)

超分子 #4: MINX introns and exons

• 超分子: 名称: MINX introns and exons / タイプ: complex / ID: 4 / 親要素: 1 / 含まれる分子: #11, #15
• 由来(天然): 生物種: Human adenovirus 2 (ヒトアデノウイルス)

分子 #1: U2 snRNA

• 分子: 名称: U2 snRNA / タイプ: rna / ID: 1 / コピー数: 1
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 60.459621 KDa

配列

文字列:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAAGAU UUUUGGAACU CAGGGAGAUG GAAUAGGAGC UUGCUCCGUC CACUCCACGC AUCGACCUGG U AUUGCAGU ACCUCCAGGA ACGGUGCACC C

分子 #2: U5 snRNA

• 分子: 名称: U5 snRNA / タイプ: rna / ID: 2 / コピー数: 1
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 36.908668 KDa

配列

文字列:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

分子 #3: U6 snRNA

• 分子: 名称: U6 snRNA / タイプ: rna / ID: 3 / コピー数: 1
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 34.09827 KDa

配列

文字列:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

分子 #11: Ligated exons: MINX mRNA

• 分子: 名称: Ligated exons: MINX mRNA / タイプ: rna / ID: 11 / コピー数: 1
• 由来(天然): 生物種: Human adenovirus 2 (ヒトアデノウイルス)
• 分子量: 理論値: 4.4377 KDa

配列

文字列:

GGCCUCCGAA CGCU

分子 #15: Intron lariat: MINX RNA

• 分子: 名称: Intron lariat: MINX RNA / タイプ: rna / ID: 15 / コピー数: 1
• 由来(天然): 生物種: Human adenovirus 2 (ヒトアデノウイルス)
• 分子量: 理論値: 27.772039 KDa

配列

文字列:

GUAAGAGCCU AGCAUGUAGA A(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)UGAUGAUG UCAUACUUAU CCU(N)(N)(N)(N)(N) (N) (N)(N)(N)CAG

分子 #4: Eukaryotic initiation factor 4A-III, N-terminally processed

• 分子: 名称: Eukaryotic initiation factor 4A-III, N-terminally processed; タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 44.691359 KDa

配列

文字列:

DMTKVEFETS EEVDVTPTFD TMGLREDLLR GIYAYGFEKP SAIQQRAIKQ IIKGRDVIAQ SQSGTGKTAT FSISVLQCLD IQVRETQAL ILAPTRELAV QIQKGLLALG DYMNVQCHAC IGGTNVGEDI RKLDYGQHVV AGTPGRVFDM IRRRSLRTRA I KMLVLDEA DEMLNKGFKE QIYDVYRYLP PATQVVLISA TLPHEILEMT NKFMTDPIRI LVKRDELTLE GIKQFFVAVE RE EWKFDTL CDLYDTLTIT QAVIFCNTKR KVDWLTEKMR EANFTVSSMH GDMPQKERES IMKEFRSGAS RVLISTDVWA RGL DVPQVS LIINYDLPNN RELYIHRIGR SGRYGRKGVA INFVKNDDIR ILRDIEQYYS TQIDEMPMNV ADLI

UniProtKB: Eukaryotic initiation factor 4A-III

分子 #5: RNA-binding protein 8A

• 分子: 名称: RNA-binding protein 8A / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 10.370526 KDa

配列

文字列:

PGPQRSVEGW ILFVTGVHEE ATEEDIHDKF AEYGEIKNIH LNLDRRTGYL KGYTLVEYET YKEAQAAMEG LNGQDLMGQP ISVDWCFVR GP

UniProtKB: RNA-binding protein 8A

分子 #6: Protein mago nashi homolog 2

• 分子: 名称: Protein mago nashi homolog 2 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 16.929314 KDa

配列

文字列:

SDFYLRYYVG HKGKFGHEFL EFEFRPDGKL RYANNSNYKN DVMIRKEAYV HKSVMEELKR IIDDSEITKE DDALWPPPDR VGRQELEIV IGDEHISFTT SKIGSLIDVN QSKDPEGLRV FYYLVQDLKC LVFSLIGLHF KIKPI

UniProtKB: Protein mago nashi homolog 2

分子 #7: Pre-mRNA-processing-splicing factor 8

• 分子: 名称: Pre-mRNA-processing-splicing factor 8 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 273.97425 KDa

配列

文字列:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

分子 #8: U5 small nuclear ribonucleoprotein 200 kDa helicase

• 分子: 名称: U5 small nuclear ribonucleoprotein 200 kDa helicase / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 197.053984 KDa

配列

文字列:

APRQVLDLED LVFTQGSHFM ANKRCQLPDG SFRRQRKGYE EVHVPALKPK PFGSEEQLLP VEKLPKYAQA GFEGFKTLNR IQSKLYRAA LETDENLLLC APTGAGKTNV ALMCMLREIG KHINMDGTIN VDDFKIIYIA PMRSLVQEMV GSFGKRLATY G ITVAELTG DHQLCKEEIS ATQIIVCTPE KWDIITRKGG ERTYTQLVRL IILDEIHLLH DDRGPVLEAL VARAIRNIEM TQ EDVRLIG LSATLPNYED VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR FQIMNEIVYE KIMEHAGKNQ VLV FVHSRK ETGKTARAIR DMCLEKDTLG LFLREGSAST EVLRTEAEQC KNLELKDLLP YGFAIHHAGM TRVDRTLVED LFAD KHIQV LVSTATLAWG VNLPAHTVII KGTQVYSPEK GRWTELGALD ILQMLGRAGR PQYDTKGEGI LITSHGELQY YLSLL NQQL PIESQMVSKL PDMLNAEIVL GNVQNAKDAV NWLGYAYLYI RMLRSPTLYG ISHDDLKGDP LLDQRRLDLV HTAALM LDK NNLVKYDKKT GNFQVTELGR IASHYYITND TVQTYNQLLK PTLSEIELFR VFSLSSEFKN ITVREEEKLE LQKLLER VP IPVKESIEEP SAKINVLLQA FISQLKLEGF ALMADMVYVT QSAGRLMRAI FEIVLNRGWA QLTDKTLNLC KMIDKRMW Q SMCPLRQFRK LPEEVVKKIE KKNFPFERLY DLNHNEIGEL IRMPKMGKTI HKYVHLFPKL ELSVHLQPIT RSTLKVELT ITPDFQWDEK VHGSSEAFWI LVEDVDSEVI LHHEYFLLKA KYAQDEHLIT FFVPVFEPLP PQYFIRVVSD RWLSCETQLP VSFRHLILP EKYPPPTELL DLQPLPVSAL RNSAFESLYQ DKFPFFNPIQ TQVFNTVYNS DDNVFVGAPT GSGKTICAEF A ILRMLLQS SEGRCVYITP MEALAEQVYM DWYEKFQDRL NKKVVLLTGE TSTDLKLLGK GNIIISTPEK WDILSRRWKQ RK NVQNINL FVVDEVHLIG GENGPVLEVI CSRMRYISSQ IERPIRIVAL SSSLSNAKDV AHWLGCSATS TFNFHPNVRP VPL ELHIQG FNISHTQTRL LSMAKPVYHA ITKHSPKKPV IVFVPSRKQT RLTAIDILTT CAADIQRQRF LHCTEKDLIP YLEK LSDST LKETLLNGVG YLHEGLSPME RRLVEQLFSS GAIQVVVASR SLCWGMNVAA HLVIIMDTQY YNGKIHAYVD YPIYD VLQM VGHANRPLQD DEGRCVIMCQ GSKKDFFKKF LYEPLPVESH LDHCMHDHFN AEIVTKTIEN KQDAVDYLTW TFLYRR MTQ NPNYYNLQGI SHRHLSDHLS ELVEQTLSDL EQSKCISIED EMDVAPLNLG MIAAYYYINY TTIELFSMSL NAKTKVR GL IEIISNAAEY ENIPIRHHED NLLRQLAQKV PHKLNNPKFN DPHVKTNLLL QAHLSRMQLS AELQSDTEEI LSKAIRLI Q ACVDVLSSNG WLSPALAAME LAQMVTQAMW SKDSYLKQLP HFTSEHIKRC TDKGVESVFD IMEMEDEERN ALLQLTDSQ IADVARFCNR YPNIELSYEV VDKDSIRSGG PVVVLVQLER EEEVTGPVIA PLFPQKREEG WWVVIGDAKS NSLISIKRLT LQQKAKVKL DFVAPATGAH NYTLYFMSDA YMGCDQEYKF SVD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

分子 #9: 116 kDa U5 small nuclear ribonucleoprotein component

• 分子: 名称: 116 kDa U5 small nuclear ribonucleoprotein component; タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 101.231938 KDa

配列

文字列:

EVVLHEDKKY YPTAEEVYGP EVETIVQEED TQPLTEPIIK PVKTKKFTLM EQTLPVTVYE MDFLADLMDN SELIRNVTLC GHLHHGKTC FVDCLIEQTH PEIRKRYDQD LCYTDILFTE QERGVGIKST PVTVVLPDTK GKSYLFNIMD TPGHVNFSDE V TAGLRISD GVVLFIDAAE GVMLNTERLI KHAVQERLAV TVCINKIDRL ILELKLPPTD AYYKLRHIVD EVNGLISMYS TD ENLILSP LLGNVCFSSS QYSICFTLGS FAKIYADTFG DINYQEFAKR LWGDIYFNPK TRKFTKKAPT SSSQRSFVEF ILE PLYKIL AQVVGDVDTS LPRTLDELGI HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH TYTG GVDSD LGEAMSDCDP DGPLMCHTTK MYSTDDGVQF HAFGRVLSGT IHAGQPVKVL GENYTLEDEE DSQICTVGRL WISVA RYHI EVNRVPAGNW VLIEGVDQPI VKTATITEPR GNEEAQIFRP LKFNTTSVIK IAVEPVNPSE LPKMLDGLRK VNKSYP SLT TKVEESGEHV ILGTGELYLD CVMHDLRKMY SEIDIKVADP VVTFCETVVE TSSLKCFAET PNKKNKITMI AEPLEKG LA EDIENEVVQI TWNRKKLGEF FQTKYDWDLL AARSIWAFGP DATGPNILVD DTLPSEVDKA LLGSVKDSIV QGFQWGTR E GPLCDELIRN VKFKILDAVV AQEPLHRGGG QIIPTARRVV YSAFLMATPR LMEPYYFVEV QAPADCVSAV YTVLARRRG HVTQDAPIPG SPLYTIKAFI PAIDSFGFET DLRTHTQGQA FSLSVFHHWQ IVPGDPLDKS IVIRPLEPQP APHLAREFMI KTRRRKGLS EDVSIKFFDD

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

分子 #10: PRKR-interacting protein 1

• 分子: 名称: PRKR-interacting protein 1 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 14.476882 KDa

配列

文字列:

TLVIPKNAAE EQKLKLERLM KNPDKAVPIP EKMSEWAPRP PPEFVRDVMG SSAGAGSGEF HVYRHLRRRE YQRQDYMDAM AEKQKLDAE FQKRLEKNKI AAEEQTAKRR KKRQKLKEKK LLAK

UniProtKB: PRKR-interacting protein 1

分子 #12: Cactin

• 分子: 名称: Cactin / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 15.210249 KDa

配列

文字列:

KYRPRKPRFF NRVHTGFAWN KYNQTHYDFD NPPPKIVQGY KFNIFYPDLI DKRSTPEYFL EACADNKDFA ILRFHAGPPY EDIAFKIVN REWEYSHRHG FRCQFANGIF QLWFHFKRYR YRR

UniProtKB: Splicing factor Cactin

分子 #13: Protein FAM32A

• 分子: 名称: Protein FAM32A / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 7.269374 KDa

配列

文字列:

RGLDKRTPAQ AAFEKMQEKR QMERILKKAS KTHKQRVEDF NRHLDTLTEH YDIPKVSWTK

UniProtKB: Protein FAM32A

分子 #14: Pre-mRNA-splicing factor CWC22 homolog

• 分子: 名称: Pre-mRNA-splicing factor CWC22 homolog / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 105.646578 KDa

配列

文字列:

MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRLI LN FRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV SPRGINAIFE RLR NILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MDPNFMENEE KYKA IKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLDFEECAHK LLKME FPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVAKMFAHL LYTDSL PWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAINFFTS IGLGGLT DE LREHLKNTPK VIVAQKPDVE QNKSSPSSSS SASSSSESDS SDSDSDSSDS SSESSSEESD SSSISSHSSA SANDVRKK G HGKTRSKEVD KLIRNQQTND RKQKERRQEH GHQETRTERE RRSEKHRDQN SSGSNWRDPI TKYTSDKDVP SERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQS RESKKNQDRR REKSPAKQK

UniProtKB: Pre-mRNA-splicing factor CWC22 homolog

分子 #16: Pleiotropic regulator 1

• 分子: 名称: Pleiotropic regulator 1 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 35.782395 KDa

配列

文字列:

MPKPQWHPPW KLYRVISGHL GWVRCIAVEP GNQWFVTGSA DRTIKIWDLA SGKLKLSLTG HISTVRGVIV STRSPYLFSC GEDKQVKCW DLEYNKVIRH YHGHLSAVYG LDLHPTIDVL VTCSRDSTAR IWDVRTKASV HTLSGHTNAV ATVRCQAAEP Q IITGSHDT TIRLWDLVAG KTRVTLTNHK KSVRAVVLHP RHYTFASGSP DNIKQWKFPD GSFIQNLSGH NAIINTLTVN SD GVLVSGA DNGTMHLWDW RTGYNFQRVH AAVQPGSLDS ESGIFACAFD QSESRLLTAE ADKTIKVYRE DDTATEETHP VSW

UniProtKB: Pleiotropic regulator 1

分子 #17: SNW domain-containing protein 1

• 分子: 名称: SNW domain-containing protein 1 / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 33.819922 KDa

配列

文字列:

PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQV DSEGKIKYDA IARQGQSKDK VIYSKYTDLV PKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV AAAMPVRAAD KLAPAQYIRY TPSQQGVAFN SGAKQRVIRM V EMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP)PSRKMT VKEQQEWKIP PCISNWKNAK GYTIPLDKRL AADG RGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKHEEKLREM AQKARERR

UniProtKB: SNW domain-containing protein 1

分子 #18: Protein BUD31 homolog

• 分子: 名称: Protein BUD31 homolog / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 17.03285 KDa

配列

文字列:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

分子 #19: Pre-mRNA-splicing factor RBM22

• 分子: 名称: Pre-mRNA-splicing factor RBM22 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 33.156129 KDa

配列

文字列:

ADFPILCQTC LGENPYIRMT KEKYGKECKI CARPFTVFRW CPGVRMRFKK TEVCQTCSKL KNVCQTCLLD LEYGLPIQVR DAGLSFKDD MPKSDVNKEY YTQNMEREIS NSDGTRPVGM LGKATSAAAA LLKLARTTPY YKRNRPHICS FWVKGECKRG E ECPYRHEK PTDPDDPLAD QNIKDRYYGI NDPVADKLLK RASTMPRLDP PEDKTITTLY VGGLGDTITE TDLRNHFYQF GE IRTITVV QRQQCAFIQF ATRQAAEVAA EKSFNKLIVN GRRLNVKWGR SQ

UniProtKB: Pre-mRNA-splicing factor RBM22

分子 #20: U5 small nuclear ribonucleoprotein 40 kDa protein

• 分子: 名称: U5 small nuclear ribonucleoprotein 40 kDa protein / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 34.09241 KDa

配列

文字列:

RCSSLQPIML LSGHEGEVYC CKFHPNGSTL ASAGFDRLIL LWNVYGDCDN YATLKGHSGA VMELHYNTDG SMLFSASTDK TVAVWDSET GERVKRLKGH TSFVNSCYPA RRGPQLVCTG SDDGTVKLWD IRKKAAIQTF QNTYQVLAVT FNDTSDQIIS G GIDNDIKV WDLRQNKLTY TMRGHADSVT GLSLSSEGSY LLSNAMDNTV RVWDVRPFAP KERCVKIFQG NVHNFEKNLL RC SWSPDGS KIAAGSADRF VYVWDTTSRR ILYKLPGHAG SINEVAFHPD EPIIISASSD KRLYMGEIQ

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

分子 #21: Cell division cycle 5-like protein

• 分子: 名称: Cell division cycle 5-like protein / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 92.406883 KDa

配列

文字列:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

分子 #22: Spliceosome-associated protein CWC15 homolog

• 分子: 名称: Spliceosome-associated protein CWC15 homolog / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 26.674447 KDa

配列

文字列:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

分子 #23: Serine/arginine repetitive matrix protein 2

• 分子: 名称: Serine/arginine repetitive matrix protein 2 / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 2.767128 KDa

配列

文字列:

MYNGIGLPTP RGSGTNGYVQ RNLSLV

UniProtKB: Serine/arginine repetitive matrix protein 2

分子 #24: Crooked neck-like protein 1

• 分子: 名称: Crooked neck-like protein 1 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 100.610008 KDa

配列

文字列:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

分子 #25: Pre-mRNA-splicing factor SYF1

• 分子: 名称: Pre-mRNA-splicing factor SYF1 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 100.148711 KDa

配列

文字列:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

UniProtKB: Pre-mRNA-splicing factor SYF1

分子 #26: Intron-binding protein aquarius

• 分子: 名称: Intron-binding protein aquarius / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 171.502453 KDa

配列

文字列:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

UniProtKB: RNA helicase aquarius

分子 #27: ATP-dependent RNA helicase DHX8

• 分子: 名称: ATP-dependent RNA helicase DHX8 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 139.522312 KDa
• 組換発現: 生物種: Saccharomyces cerevisiae (パン酵母)

配列

文字列:

MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLI QTMRPPAKPS TSKDPVVKPK TEKEKLKELF PVLCQPDNPS VRTMLDEDDV KVAVDVLKEL EALMPSAAGQ E KQRDAEHR DRTKKKKRSR SRDRNRDRDR DRERNRDRDH KRRHRSRSRS RSRTRERNKV KSRYRSRSRS QSPPKDRKDR DK YGERNLD RWRDKHVDRP PPEEPTIGDI YNGKVTSIMQ FGCFVQLEGL RKRWEGLVHI SELRREGRVA NVADVVSKGQ RVK VKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKW EIKQM IAANVLSKEE FPDFDEETGI LPKVDDEEDE DLEIELVEEE PPFLRGHTKQ SMDMSPIKIV KNPDGSLSQA AMMQS ALAK ERRELKQAQR EAEMDSIPMG LNKHWVDPLP DAEGRQIAAN MRGIGMMPND IPEWKKHAFG GNKASYGKKT QMSILE QRE SLPIYKLKEQ LVQAVHDNQI LIVIGETGSG KTTQITQYLA EAGYTSRGKI GCTQPRRVAA MSVAKRVSEE FGCCLGQ EV GYTIRFEDCT SPETVIKYMT DGMLLRECLI DPDLTQYAII MLDEAHERTI HTDVLFGLLK KTVQKRQDMK LIVTSATL D AVKFSQYFYE APIFTIPGRT YPVEILYTKE PETDYLDASL ITVMQIHLTE PPGDILVFLT GQEEIDTACE ILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAG RAGRTGPGKC YRLYTERAYR DEMLTTNVPE IQRTNLASTV LSLKAMGIND LLSFDFMDAP PMETLITAME Q LYTLGALD DEGLLTRLGR RMAEFPLEPM LCKMLIMSVH LGCSEEMLTI VSMLSVQNVF YRPKDKQALA DQKKAKFHQT EG DHLTLLA VYNSWKNNKF SNPWCYENFI QARSLRRAQD IRKQMLGIMD RHKLDVVSCG KSTVRVQKAI CSGFFRNAAK KDP QEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEP LYNRY EEPNAWRISR AFRRR

UniProtKB: ATP-dependent RNA helicase DHX8

分子 #28: U2 small nuclear ribonucleoprotein A'

• 分子: 名称: U2 small nuclear ribonucleoprotein A' / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 18.69773 KDa

配列

文字列:

VKLTAELIEQ AAQYTNAVRD RELDLRGYKI PVIENLGATL DQFDAIDFSD NEIRKLDGFP LLRRLKTLLV NNNRICRIGE GLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK M FK

UniProtKB: U2 small nuclear ribonucleoprotein A'

分子 #29: U2 small nuclear ribonucleoprotein B''

• 分子: 名称: U2 small nuclear ribonucleoprotein B'' / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 10.688563 KDa

配列

文字列:

IRPNHTIYIN NMNDKIKKEE LKRSLYALFS QFGHVVDIVA LKTMKMRGQA FVIFKELGSS TNALRQLQGF PFYGKPMRIQ YAKTDSDII SKM

UniProtKB: U2 small nuclear ribonucleoprotein B''

分子 #30: NF-kappa-B-activating protein

• 分子: 名称: NF-kappa-B-activating protein / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 3.321766 KDa

配列

文字列:

KRIPRRGEIG LTSEEIASFE CSGYVMSGSR

UniProtKB: NF-kappa-B-activating protein

分子 #31: Small nuclear ribonucleoprotein-associated proteins B and B'

• 分子: 名称: Small nuclear ribonucleoprotein-associated proteins B and B'; タイプ: protein_or_peptide / ID: 31 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 9.551284 KDa

配列

文字列:

SSKMLQHIDY RMRCILQDGR IFIGTFKAFD KHMNLILCDC DEFRKIKPKN SKQAEREEKR VLGLVLLRGE NLVSMTVEGP PP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

分子 #32: Pre-mRNA-splicing factor SLU7

• 分子: 名称: Pre-mRNA-splicing factor SLU7 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 68.510844 KDa

配列

文字列:

MSATVVDAVN AAPLSGSKEM SLEEPKKMTR EDWRKKKELE EQRKLGNAPA EVDEEGKDIN PHIPQYISSV PWYIDPSKRP TLKHQRPQP EKQKQFSSSG EWYKRGVKEN SIITKYRKGA CENCGAMTHK KKDCFERPRR VGAKFTGTNI APDEHVQPQL M FDYDGKRD RWNGYNPEEH MKIVEEYAKV DLAKRTLKAQ KLQEELASGK LVEQANSPKH QWGEEEPNSQ MEKDHNSEDE DE DKYADDI DMPGQNFDSK RRITVRNLRI REDIAKYLRN LDPNSAYYDP KTRAMRENPY ANAGKNPDEV SYAGDNFVRY TGD TISMAQ TQLFAWEAYD KGSEVHLQAD PTKLELLYKS FKVKKEDFKE QQKESILEKY GGQEHLDAPP AELLLAQTED YVEY SRHGT VIKGQERAVA CSKYEEDVKI HNHTHIWGSY WKEGRWGYKC CHSFFKYSYC TGEAGKEIVN SEECIINEIT GEESV KKPQ TLMELHQEKL KEEKKKKKKK KKKHRKSSSD SDDEEKKHEK LKKALNAEEA RLLHVKETMQ IDERKRPYNS MYETRE PTE EEMEAYRMKR QRPDDPMASF LGQ

UniProtKB: Pre-mRNA-splicing factor SLU7

分子 #33: Small nuclear ribonucleoprotein Sm D3

• 分子: 名称: Small nuclear ribonucleoprotein Sm D3 / タイプ: protein_or_peptide / ID: 33 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 9.460979 KDa

配列

文字列:

SIGVPIKVLH EAEGHIVTCE TNTGEVYRGK LIEAEDNMNC QMSNITVTYR DGRVAQLEQV YIRGSKIRFL ILPDMLKNAP MLKS

UniProtKB: Small nuclear ribonucleoprotein Sm D3

分子 #34: Small nuclear ribonucleoprotein E

• 分子: 名称: Small nuclear ribonucleoprotein E / タイプ: protein_or_peptide / ID: 34 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 9.582185 KDa

配列

文字列:

QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV S

UniProtKB: Small nuclear ribonucleoprotein E

分子 #35: Small nuclear ribonucleoprotein F

• 分子: 名称: Small nuclear ribonucleoprotein F / タイプ: protein_or_peptide / ID: 35 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 8.038415 KDa

配列

文字列:

PLNPKPFLNG LTGKPVMVKL KWGMEYKGYL VSVDGYMNMQ LANTEEYIDG ALSGHLGEVL IRCNNVLYIR GV

UniProtKB: Small nuclear ribonucleoprotein F

分子 #36: Small nuclear ribonucleoprotein G

• 分子: 名称: Small nuclear ribonucleoprotein G / タイプ: protein_or_peptide / ID: 36 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 8.160629 KDa

配列

文字列:

AHPPELKKFM DKKLSLKLNG GRHVQGILRG FDPFMNLVID ECVEMATSGQ QNNIGMVVIR GNSIIMLEAL ERV

UniProtKB: Small nuclear ribonucleoprotein G

分子 #37: Small nuclear ribonucleoprotein Sm D1

• 分子: 名称: Small nuclear ribonucleoprotein Sm D1 / タイプ: protein_or_peptide / ID: 37 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 9.086731 KDa

配列

文字列:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL

UniProtKB: Small nuclear ribonucleoprotein Sm D1

分子 #38: Peptidyl-prolyl cis-trans isomerase-like 1

• 分子: 名称: Peptidyl-prolyl cis-trans isomerase-like 1 / タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO / EC番号: peptidylprolyl isomerase
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 18.055529 KDa

配列

文字列:

AIPPDSWQPP NVYLETSMGI IVLELYWKHA PKTCKNFAEL ARRGYYNGTK FHRIIKDFMI QGGDPTGTGR GGASIYGKQF EDELHPDLK FTGAGILAMA NAGPDTNGSQ FFVTLAPTQW LDGKHTIFGR VCQGIGMVNR VGMVETNSQD RPVDDVKIIK A YPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

分子 #39: Small nuclear ribonucleoprotein Sm D2

• 分子: 名称: Small nuclear ribonucleoprotein Sm D2 / タイプ: protein_or_peptide / ID: 39 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 13.551928 KDa

配列

文字列:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

分子 #40: Pre-mRNA-processing factor 17

• 分子: 名称: Pre-mRNA-processing factor 17 / タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 59.116055 KDa

配列

文字列:

ETGVHLDPAV KEVQYNPTYE TMFAPEFGPE NPFRTQQMAA PRNMLSGYAE PAHINDFMFE QQRRTFATYG YALDPSLDNH QVSAKYIGS VEEAEKNQGL TVFETGQKKT EKRKKFKEND ASNIDGFLGP WAKYVDEKDV AKPSEEEQKE LDEITAKRQK K GKQEEEKP GEEKTILHVK EMYDYQGRSY LHIPQDVGVN LRSTMPPEKC YLPKKQIHVW SGHTKGVSAV RLFPLSGHLL LS CSMDCKI KLWEVYGERR CLRTFIGHSK AVRDICFNTA GTQFLSAAYD RYLKLWDTET GQCISRFTNR KVPYCVKFNP DED KQNLFV AGMSDKKIVQ WDIRSGEIVQ EYDRHLGAVN TIVFVDENRR FVSTSDDKSL RVWEWDIPVD FKYIAEPSMH SMPA VTLSP NGKWLACQSM DNQILIFGAQ NRFRLNKKKI FKGHMVAGYA CQVDFSPDMS YVISGDGNGK LNIWDWKTTK LYSRF KAHD KVCIGAVWHP HETSKVITCG WDGLIKLWD

UniProtKB: Pre-mRNA-processing factor 17

分子 #41: Pre-mRNA-splicing factor SPF27

• 分子: 名称: Pre-mRNA-splicing factor SPF27 / タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 26.16342 KDa

配列

文字列:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

UniProtKB: Pre-mRNA-splicing factor SPF27

分子 #42: Pre-mRNA-processing factor 19

• 分子: 名称: Pre-mRNA-processing factor 19 / タイプ: protein_or_peptide / ID: 42 / コピー数: 4 / 光学異性体: LEVO / EC番号: RING-type E3 ubiquitin transferase
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 55.245547 KDa

配列

文字列:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

UniProtKB: Pre-mRNA-processing factor 19

分子 #43: Pre-mRNA-splicing factor SYF2

• 分子: 名称: Pre-mRNA-splicing factor SYF2 / タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 17.302371 KDa

配列

文字列:

LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE FFPTSNSLLH GTHVPSTEEI DRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RGTAV

UniProtKB: Pre-mRNA-splicing factor SYF2

分子 #44: Replication stress response regulator SDE2

• 分子: 名称: Replication stress response regulator SDE2 / タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Homo sapiens (ヒト)
• 分子量: 理論値: 4.036538 KDa

配列

文字列:

EKTTNREACR DLSGRRLRDV NHEKAMAEWV KQQA

UniProtKB: Splicing regulator SDE2

分子 #45: MAGNESIUM ION

• 分子: 名称: MAGNESIUM ION / タイプ: ligand / ID: 45 / コピー数: 7 / 式: MG
• 分子量: 理論値: 24.305 Da

分子 #46: POTASSIUM ION

• 分子: 名称: POTASSIUM ION / タイプ: ligand / ID: 46 / コピー数: 1 / 式: K
• 分子量: 理論値: 39.098 Da

分子 #47: ADENOSINE-5'-TRIPHOSPHATE

• 分子: 名称: ADENOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 47 / コピー数: 1 / 式: ATP
• 分子量: 理論値: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM

分子 #48: GUANOSINE-5'-TRIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 48 / コピー数: 1 / 式: GTP
• 分子量: 理論値: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM

分子 #49: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 49 / コピー数: 7 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #50: INOSITOL HEXAKISPHOSPHATE

• 分子: 名称: INOSITOL HEXAKISPHOSPHATE / タイプ: ligand / ID: 50 / コピー数: 1 / 式: IHP
• 分子量: 理論値: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / myo-イノシト-ル六りん酸

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 濃度: 0.3 mg/mL

緩衝液

pH: 7.9
構成要素:

濃度	名称	式
20.0 mM	HEPES
100.0 mM		KCl
0.2 mM	EDTA

• グリッド: モデル: Quantifoil R2/2 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 7 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 15 sec. / 前処理 - 雰囲気: AIR
• 凍結: 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK III; 詳細: 3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane..

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 撮影: フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k); 検出モード: COUNTING / デジタル化 - 画像ごとのフレーム数: 1-40 / 撮影したグリッド数: 2 / 実像数: 6200 / 平均露光時間: 10.0 sec. / 平均電子線量: 53.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 3.5 µm / 最小 デフォーカス（公称値）: 1.5 µm / 倍率（公称値）: 135000
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

• 初期モデル: モデルのタイプ: OTHER
• 最終 再構成: 想定した対称性 - 点群: C₁ (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.3 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3.0) / 使用した粒子像数: 103860
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0)
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0)