EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K5...

Basic information

• Entry: Database: EMDB / ID: EMD-4525
• Title: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• Map data: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map

Sample

• Complex: Human post-catalytic P complex spliceosome
  • Complex: Human post-catalytic P complex spliceosome
    • RNA: x 3 types
    • Protein or peptide: x 38 types
  • Complex: ATP-dependent RNA helicase DHX8
    • Protein or peptide: x 1 types
  • Complex: MINX introns and exons
    • RNA: x 2 types
• Ligand: x 6 types

• Keywords: spliceosome / RNA / complex / SPLICING

Function / homology

Function:

regulation of homologous chromosome segregation / second spliceosomal transesterification activity / endoplasmic reticulum membrane organization / exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / negative regulation of nitric-oxide synthase activity / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / post-spliceosomal complex / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / pre-mRNA 3'-splice site binding / NOSIP mediated eNOS trafficking / protein exit from endoplasmic reticulum / negative regulation of catalytic activity / regulation of translation at postsynapse, modulating synaptic transmission / regulation of retinoic acid receptor signaling pathway / granulocyte differentiation / post-mRNA release spliceosomal complex / renal system process / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / 3'-5' RNA helicase activity / U7 snRNP / negative regulation of toll-like receptor signaling pathway / generation of catalytic spliceosome for first transesterification step / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / regulation of mRNA processing / regulation of vitamin D receptor signaling pathway / negative regulation of excitatory postsynaptic potential / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of interleukin-8 production / SLBP independent Processing of Histone Pre-mRNAs / negative regulation of lipopolysaccharide-mediated signaling pathway / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Deadenylation of mRNA / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / nuclear retinoic acid receptor binding / embryonic brain development / protein methylation / embryonic cranial skeleton morphogenesis / oocyte development / U12-type spliceosomal complex / alternative mRNA splicing, via spliceosome / poly(A) binding / 7-methylguanosine cap hypermethylation / negative regulation of interferon-beta production / U1 snRNP binding / methylosome / mRNA 3'-end processing / M-decay: degradation of maternal mRNAs by maternally stored factors / RNA splicing, via transesterification reactions / ATP-dependent activity, acting on RNA / pICln-Sm protein complex / C2H2 zinc finger domain binding / U2-type catalytic step 1 spliceosome / pre-mRNA binding / regulation of mRNA splicing, via spliceosome / snRNP binding / positive regulation of mRNA splicing, via spliceosome / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / small nuclear ribonucleoprotein complex / spliceosomal tri-snRNP complex / host-mediated activation of viral transcription / P granule / telomerase holoenzyme complex / U2-type precatalytic spliceosome / positive regulation of vitamin D receptor signaling pathway / commitment complex / mRNA cis splicing, via spliceosome / telomerase RNA binding / U2-type spliceosomal complex / regulation of nitric oxide biosynthetic process / nuclear vitamin D receptor binding / U2-type prespliceosome assembly / Notch binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / positive regulation of alpha-beta T cell differentiation / U2-type catalytic step 2 spliceosome / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / positive regulation of neurogenesis / NOTCH4 Intracellular Domain Regulates Transcription / RNA Polymerase II Transcription Termination / U2 snRNP / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / U1 snRNP / U4 snRNP / NOTCH3 Intracellular Domain Regulates Transcription / negative regulation of type I interferon-mediated signaling pathway / U2-type prespliceosome / inner cell mass cell proliferation / WD40-repeat domain binding / ubiquitin-ubiquitin ligase activity / protein peptidyl-prolyl isomerization / K63-linked polyubiquitin modification-dependent protein binding

Domain/homology:

XAP5 protein / Nitric oxide synthase-interacting protein / STEEP-like / Nitric oxide synthase-interacting protein, zinc-finger / : / XAP5, circadian clock regulator C-terminal domain / Zinc-finger of nitric oxide synthase-interacting protein / Telomere length and silencing protein 1 / Hepatocellular carcinoma-associated antigen 59 / NF-kappa-B-activating protein, C-terminal / NF-kappa-B-activating protein / NF-kappa-B-activating protein C-terminal domain / NF-kappa-B-activating protein / Nuclear protein DGCR14/ESS-2 / : / Nuclear protein Es2 / Vertebrate Splicing regulator sde2, N-terminal ubiquitin domain / Protein FAM32A / Cactin, central domain / Cactin, C-terminal / FAM32A / Cactus-binding C-terminus of cactin protein / Conserved mid region of cactin / PRKR-interacting protein 1 / Protein of unknown function (DUF1168) / mRNA splicing factor Cwf18-like / : / cwf18 pre-mRNA splicing factor / Splicing regulator SDE2, SDE2 domain / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / : / : / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / : / Prp19 WD40 domain / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Peptidyl-prolyl cis-trans isomerase E / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / mRNA splicing factor SYF2 / SYF2 splicing factor / Cactus-binding C-terminus of cactin protein / Helix hairpin bin domain superfamily / : / Replication stress response SDE2 C-terminal / : / : / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / : / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / : / Prp19/Pso4-like / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / G10 protein signature 2. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / U-box domain / G10 protein signature 1. / : / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / DNA2/NAM7 helicase, helicase domain / : / AAA domain / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / : / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal

Component:

Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor SYF2 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Eukaryotic initiation factor 4A-III / Protein BUD31 homolog / Protein mago nashi homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / Protein FAM50A / ATP-dependent RNA helicase DHX8 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing regulator SDE2 / Pre-mRNA-processing-splicing factor 8 / NF-kappa-B-activating protein / Coiled-coil domain-containing protein 12 / Splicing factor Cactin / Protein mago nashi homolog 2 / Peptidylprolyl isomerase domain and WD repeat-containing protein 1 / Splicing factor ESS-2 homolog / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Crooked neck-like protein 1 / STING ER exit protein / PRKR-interacting protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / Splicing factor C9orf78 / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor ISY1 homolog / Pre-mRNA-processing factor 19 / Peptidyl-prolyl cis-trans isomerase E / Serine/arginine repetitive matrix protein 2 / Nitric oxide synthase-interacting protein / Peptidyl-prolyl cis-trans isomerase-like 1 / Protein FAM32A / RNA-binding protein 8A

• Biological species: Homo sapiens (human) / Human adenovirus 2
• Method: single particle reconstruction / cryo EM / Resolution: 3.3 Å
• Authors: Fica SM / Oubridge C

Funding support

United Kingdom, 2 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	MC_U105184330	United Kingdom
European Research Council	AdG-693087-SPLICE3D

• Citation: Journal: Science / Year: 2019; Title: A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.; Authors: Sebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai / ; Abstract: During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.

History

Deposition	Jan 3, 2019	-
Header (metadata) release	Feb 20, 2019	-
Map release	Feb 20, 2019	-
Update	Nov 13, 2024	-
Current status	Nov 13, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4525.map.gz / Format: CCP4 / Size: 262.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• Voxel size: X=Y=Z: 1.2 Å

Density

Contour Level	By AUTHOR: 0.024 / Movie #1: 0.024
Minimum - Maximum	-0.10098974 - 0.17720254
Average (Standard dev.)	0.000077332166 (±0.0050888886)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 410 410 410 Spacing 410 410 410
Cell	A=B=C: 492.00003 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.2	1.2	1.2
M x/y/z	410	410	410
origin x/y/z	0.000	0.000	0.000
length x/y/z	492.000	492.000	492.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	364	364	364
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	410	410	410
D min/max/mean	-0.101	0.177	0.000

Supplemental data

Sample components

Entire : Human post-catalytic P complex spliceosome

• Entire: Name: Human post-catalytic P complex spliceosome

Components

• Complex: Human post-catalytic P complex spliceosome
  • Complex: Human post-catalytic P complex spliceosome
    • RNA: U2 snRNA
    • RNA: U5 snRNA
    • RNA: U6 snRNA
    • Protein or peptide: Eukaryotic initiation factor 4A-III, N-terminally processed
    • Protein or peptide: RNA-binding protein 8A
    • Protein or peptide: Protein mago nashi homolog 2
    • Protein or peptide: Pre-mRNA-processing-splicing factor 8
    • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
    • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
    • Protein or peptide: PRKR-interacting protein 1
    • Protein or peptide: Cactin
    • Protein or peptide: Protein FAM32A
    • Protein or peptide: Pre-mRNA-splicing factor CWC22 homolog
    • Protein or peptide: Pleiotropic regulator 1
    • Protein or peptide: SNW domain-containing protein 1
    • Protein or peptide: Protein BUD31 homolog
    • Protein or peptide: Pre-mRNA-splicing factor RBM22
    • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
    • Protein or peptide: Cell division cycle 5-like protein
    • Protein or peptide: Spliceosome-associated protein CWC15 homolog
    • Protein or peptide: Serine/arginine repetitive matrix protein 2
    • Protein or peptide: Crooked neck-like protein 1
    • Protein or peptide: Pre-mRNA-splicing factor SYF1
    • Protein or peptide: Intron-binding protein aquarius
    • Protein or peptide: U2 small nuclear ribonucleoprotein A'
    • Protein or peptide: U2 small nuclear ribonucleoprotein B''
    • Protein or peptide: NF-kappa-B-activating protein
    • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
    • Protein or peptide: Pre-mRNA-splicing factor SLU7
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Pre-mRNA-processing factor 17
    • Protein or peptide: Pre-mRNA-splicing factor SPF27
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Pre-mRNA-splicing factor SYF2
    • Protein or peptide: Replication stress response regulator SDE2
  • Complex: ATP-dependent RNA helicase DHX8
    • Protein or peptide: ATP-dependent RNA helicase DHX8
  • Complex: MINX introns and exons
    • RNA: Ligated exons: MINX mRNA
    • RNA: Intron lariat: MINX RNA
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: INOSITOL HEXAKISPHOSPHATE

Supramolecule #1: Human post-catalytic P complex spliceosome

• Supramolecule: Name: Human post-catalytic P complex spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Molecular weight: Theoretical: 3.0 MDa

Supramolecule #2: Human post-catalytic P complex spliceosome

• Supramolecule: Name: Human post-catalytic P complex spliceosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#10, #12-#14, #16-#26, #28-#44
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #3: ATP-dependent RNA helicase DHX8

• Supramolecule: Name: ATP-dependent RNA helicase DHX8 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #27
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #4: MINX introns and exons

• Supramolecule: Name: MINX introns and exons / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #11, #15
• Source (natural): Organism: Human adenovirus 2

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.459621 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAAGAU UUUUGGAACU CAGGGAGAUG GAAUAGGAGC UUGCUCCGUC CACUCCACGC AUCGACCUGG U AUUGCAGU ACCUCCAGGA ACGGUGCACC C

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.908668 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

Macromolecule #3: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.09827 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

Macromolecule #11: Ligated exons: MINX mRNA

• Macromolecule: Name: Ligated exons: MINX mRNA / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Human adenovirus 2
• Molecular weight: Theoretical: 4.4377 KDa

Sequence

String:

GGCCUCCGAA CGCU

Macromolecule #15: Intron lariat: MINX RNA

• Macromolecule: Name: Intron lariat: MINX RNA / type: rna / ID: 15 / Number of copies: 1
• Source (natural): Organism: Human adenovirus 2
• Molecular weight: Theoretical: 27.772039 KDa

Sequence

String:

GUAAGAGCCU AGCAUGUAGA A(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)UGAUGAUG UCAUACUUAU CCU(N)(N)(N)(N)(N) (N) (N)(N)(N)CAG

Macromolecule #4: Eukaryotic initiation factor 4A-III, N-terminally processed

• Macromolecule: Name: Eukaryotic initiation factor 4A-III, N-terminally processed; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.691359 KDa

Sequence

String:

DMTKVEFETS EEVDVTPTFD TMGLREDLLR GIYAYGFEKP SAIQQRAIKQ IIKGRDVIAQ SQSGTGKTAT FSISVLQCLD IQVRETQAL ILAPTRELAV QIQKGLLALG DYMNVQCHAC IGGTNVGEDI RKLDYGQHVV AGTPGRVFDM IRRRSLRTRA I KMLVLDEA DEMLNKGFKE QIYDVYRYLP PATQVVLISA TLPHEILEMT NKFMTDPIRI LVKRDELTLE GIKQFFVAVE RE EWKFDTL CDLYDTLTIT QAVIFCNTKR KVDWLTEKMR EANFTVSSMH GDMPQKERES IMKEFRSGAS RVLISTDVWA RGL DVPQVS LIINYDLPNN RELYIHRIGR SGRYGRKGVA INFVKNDDIR ILRDIEQYYS TQIDEMPMNV ADLI

UniProtKB: Eukaryotic initiation factor 4A-III

Macromolecule #5: RNA-binding protein 8A

• Macromolecule: Name: RNA-binding protein 8A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.370526 KDa

Sequence

String:

PGPQRSVEGW ILFVTGVHEE ATEEDIHDKF AEYGEIKNIH LNLDRRTGYL KGYTLVEYET YKEAQAAMEG LNGQDLMGQP ISVDWCFVR GP

UniProtKB: RNA-binding protein 8A

Macromolecule #6: Protein mago nashi homolog 2

• Macromolecule: Name: Protein mago nashi homolog 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.929314 KDa

Sequence

String:

SDFYLRYYVG HKGKFGHEFL EFEFRPDGKL RYANNSNYKN DVMIRKEAYV HKSVMEELKR IIDDSEITKE DDALWPPPDR VGRQELEIV IGDEHISFTT SKIGSLIDVN QSKDPEGLRV FYYLVQDLKC LVFSLIGLHF KIKPI

UniProtKB: Protein mago nashi homolog 2

Macromolecule #7: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

Macromolecule #8: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 197.053984 KDa

Sequence

String:

APRQVLDLED LVFTQGSHFM ANKRCQLPDG SFRRQRKGYE EVHVPALKPK PFGSEEQLLP VEKLPKYAQA GFEGFKTLNR IQSKLYRAA LETDENLLLC APTGAGKTNV ALMCMLREIG KHINMDGTIN VDDFKIIYIA PMRSLVQEMV GSFGKRLATY G ITVAELTG DHQLCKEEIS ATQIIVCTPE KWDIITRKGG ERTYTQLVRL IILDEIHLLH DDRGPVLEAL VARAIRNIEM TQ EDVRLIG LSATLPNYED VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR FQIMNEIVYE KIMEHAGKNQ VLV FVHSRK ETGKTARAIR DMCLEKDTLG LFLREGSAST EVLRTEAEQC KNLELKDLLP YGFAIHHAGM TRVDRTLVED LFAD KHIQV LVSTATLAWG VNLPAHTVII KGTQVYSPEK GRWTELGALD ILQMLGRAGR PQYDTKGEGI LITSHGELQY YLSLL NQQL PIESQMVSKL PDMLNAEIVL GNVQNAKDAV NWLGYAYLYI RMLRSPTLYG ISHDDLKGDP LLDQRRLDLV HTAALM LDK NNLVKYDKKT GNFQVTELGR IASHYYITND TVQTYNQLLK PTLSEIELFR VFSLSSEFKN ITVREEEKLE LQKLLER VP IPVKESIEEP SAKINVLLQA FISQLKLEGF ALMADMVYVT QSAGRLMRAI FEIVLNRGWA QLTDKTLNLC KMIDKRMW Q SMCPLRQFRK LPEEVVKKIE KKNFPFERLY DLNHNEIGEL IRMPKMGKTI HKYVHLFPKL ELSVHLQPIT RSTLKVELT ITPDFQWDEK VHGSSEAFWI LVEDVDSEVI LHHEYFLLKA KYAQDEHLIT FFVPVFEPLP PQYFIRVVSD RWLSCETQLP VSFRHLILP EKYPPPTELL DLQPLPVSAL RNSAFESLYQ DKFPFFNPIQ TQVFNTVYNS DDNVFVGAPT GSGKTICAEF A ILRMLLQS SEGRCVYITP MEALAEQVYM DWYEKFQDRL NKKVVLLTGE TSTDLKLLGK GNIIISTPEK WDILSRRWKQ RK NVQNINL FVVDEVHLIG GENGPVLEVI CSRMRYISSQ IERPIRIVAL SSSLSNAKDV AHWLGCSATS TFNFHPNVRP VPL ELHIQG FNISHTQTRL LSMAKPVYHA ITKHSPKKPV IVFVPSRKQT RLTAIDILTT CAADIQRQRF LHCTEKDLIP YLEK LSDST LKETLLNGVG YLHEGLSPME RRLVEQLFSS GAIQVVVASR SLCWGMNVAA HLVIIMDTQY YNGKIHAYVD YPIYD VLQM VGHANRPLQD DEGRCVIMCQ GSKKDFFKKF LYEPLPVESH LDHCMHDHFN AEIVTKTIEN KQDAVDYLTW TFLYRR MTQ NPNYYNLQGI SHRHLSDHLS ELVEQTLSDL EQSKCISIED EMDVAPLNLG MIAAYYYINY TTIELFSMSL NAKTKVR GL IEIISNAAEY ENIPIRHHED NLLRQLAQKV PHKLNNPKFN DPHVKTNLLL QAHLSRMQLS AELQSDTEEI LSKAIRLI Q ACVDVLSSNG WLSPALAAME LAQMVTQAMW SKDSYLKQLP HFTSEHIKRC TDKGVESVFD IMEMEDEERN ALLQLTDSQ IADVARFCNR YPNIELSYEV VDKDSIRSGG PVVVLVQLER EEEVTGPVIA PLFPQKREEG WWVVIGDAKS NSLISIKRLT LQQKAKVKL DFVAPATGAH NYTLYFMSDA YMGCDQEYKF SVD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

Macromolecule #9: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 101.231938 KDa

Sequence

String:

EVVLHEDKKY YPTAEEVYGP EVETIVQEED TQPLTEPIIK PVKTKKFTLM EQTLPVTVYE MDFLADLMDN SELIRNVTLC GHLHHGKTC FVDCLIEQTH PEIRKRYDQD LCYTDILFTE QERGVGIKST PVTVVLPDTK GKSYLFNIMD TPGHVNFSDE V TAGLRISD GVVLFIDAAE GVMLNTERLI KHAVQERLAV TVCINKIDRL ILELKLPPTD AYYKLRHIVD EVNGLISMYS TD ENLILSP LLGNVCFSSS QYSICFTLGS FAKIYADTFG DINYQEFAKR LWGDIYFNPK TRKFTKKAPT SSSQRSFVEF ILE PLYKIL AQVVGDVDTS LPRTLDELGI HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH TYTG GVDSD LGEAMSDCDP DGPLMCHTTK MYSTDDGVQF HAFGRVLSGT IHAGQPVKVL GENYTLEDEE DSQICTVGRL WISVA RYHI EVNRVPAGNW VLIEGVDQPI VKTATITEPR GNEEAQIFRP LKFNTTSVIK IAVEPVNPSE LPKMLDGLRK VNKSYP SLT TKVEESGEHV ILGTGELYLD CVMHDLRKMY SEIDIKVADP VVTFCETVVE TSSLKCFAET PNKKNKITMI AEPLEKG LA EDIENEVVQI TWNRKKLGEF FQTKYDWDLL AARSIWAFGP DATGPNILVD DTLPSEVDKA LLGSVKDSIV QGFQWGTR E GPLCDELIRN VKFKILDAVV AQEPLHRGGG QIIPTARRVV YSAFLMATPR LMEPYYFVEV QAPADCVSAV YTVLARRRG HVTQDAPIPG SPLYTIKAFI PAIDSFGFET DLRTHTQGQA FSLSVFHHWQ IVPGDPLDKS IVIRPLEPQP APHLAREFMI KTRRRKGLS EDVSIKFFDD

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #10: PRKR-interacting protein 1

• Macromolecule: Name: PRKR-interacting protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.476882 KDa

Sequence

String:

TLVIPKNAAE EQKLKLERLM KNPDKAVPIP EKMSEWAPRP PPEFVRDVMG SSAGAGSGEF HVYRHLRRRE YQRQDYMDAM AEKQKLDAE FQKRLEKNKI AAEEQTAKRR KKRQKLKEKK LLAK

UniProtKB: PRKR-interacting protein 1

Macromolecule #12: Cactin

• Macromolecule: Name: Cactin / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.210249 KDa

Sequence

String:

KYRPRKPRFF NRVHTGFAWN KYNQTHYDFD NPPPKIVQGY KFNIFYPDLI DKRSTPEYFL EACADNKDFA ILRFHAGPPY EDIAFKIVN REWEYSHRHG FRCQFANGIF QLWFHFKRYR YRR

UniProtKB: Splicing factor Cactin

Macromolecule #13: Protein FAM32A

• Macromolecule: Name: Protein FAM32A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.269374 KDa

Sequence

String:

RGLDKRTPAQ AAFEKMQEKR QMERILKKAS KTHKQRVEDF NRHLDTLTEH YDIPKVSWTK

UniProtKB: Protein FAM32A

Macromolecule #14: Pre-mRNA-splicing factor CWC22 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 homolog / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 105.646578 KDa

Sequence

String:

MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRLI LN FRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV SPRGINAIFE RLR NILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MDPNFMENEE KYKA IKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLDFEECAHK LLKME FPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVAKMFAHL LYTDSL PWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAINFFTS IGLGGLT DE LREHLKNTPK VIVAQKPDVE QNKSSPSSSS SASSSSESDS SDSDSDSSDS SSESSSEESD SSSISSHSSA SANDVRKK G HGKTRSKEVD KLIRNQQTND RKQKERRQEH GHQETRTERE RRSEKHRDQN SSGSNWRDPI TKYTSDKDVP SERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQS RESKKNQDRR REKSPAKQK

UniProtKB: Pre-mRNA-splicing factor CWC22 homolog

Macromolecule #16: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.782395 KDa

Sequence

String:

MPKPQWHPPW KLYRVISGHL GWVRCIAVEP GNQWFVTGSA DRTIKIWDLA SGKLKLSLTG HISTVRGVIV STRSPYLFSC GEDKQVKCW DLEYNKVIRH YHGHLSAVYG LDLHPTIDVL VTCSRDSTAR IWDVRTKASV HTLSGHTNAV ATVRCQAAEP Q IITGSHDT TIRLWDLVAG KTRVTLTNHK KSVRAVVLHP RHYTFASGSP DNIKQWKFPD GSFIQNLSGH NAIINTLTVN SD GVLVSGA DNGTMHLWDW RTGYNFQRVH AAVQPGSLDS ESGIFACAFD QSESRLLTAE ADKTIKVYRE DDTATEETHP VSW

UniProtKB: Pleiotropic regulator 1

Macromolecule #17: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.819922 KDa

Sequence

String:

PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQV DSEGKIKYDA IARQGQSKDK VIYSKYTDLV PKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV AAAMPVRAAD KLAPAQYIRY TPSQQGVAFN SGAKQRVIRM V EMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP)PSRKMT VKEQQEWKIP PCISNWKNAK GYTIPLDKRL AADG RGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKHEEKLREM AQKARERR

UniProtKB: SNW domain-containing protein 1

Macromolecule #18: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #19: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.156129 KDa

Sequence

String:

ADFPILCQTC LGENPYIRMT KEKYGKECKI CARPFTVFRW CPGVRMRFKK TEVCQTCSKL KNVCQTCLLD LEYGLPIQVR DAGLSFKDD MPKSDVNKEY YTQNMEREIS NSDGTRPVGM LGKATSAAAA LLKLARTTPY YKRNRPHICS FWVKGECKRG E ECPYRHEK PTDPDDPLAD QNIKDRYYGI NDPVADKLLK RASTMPRLDP PEDKTITTLY VGGLGDTITE TDLRNHFYQF GE IRTITVV QRQQCAFIQF ATRQAAEVAA EKSFNKLIVN GRRLNVKWGR SQ

UniProtKB: Pre-mRNA-splicing factor RBM22

Macromolecule #20: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.09241 KDa

Sequence

String:

RCSSLQPIML LSGHEGEVYC CKFHPNGSTL ASAGFDRLIL LWNVYGDCDN YATLKGHSGA VMELHYNTDG SMLFSASTDK TVAVWDSET GERVKRLKGH TSFVNSCYPA RRGPQLVCTG SDDGTVKLWD IRKKAAIQTF QNTYQVLAVT FNDTSDQIIS G GIDNDIKV WDLRQNKLTY TMRGHADSVT GLSLSSEGSY LLSNAMDNTV RVWDVRPFAP KERCVKIFQG NVHNFEKNLL RC SWSPDGS KIAAGSADRF VYVWDTTSRR ILYKLPGHAG SINEVAFHPD EPIIISASSD KRLYMGEIQ

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

Macromolecule #21: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #22: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #23: Serine/arginine repetitive matrix protein 2

• Macromolecule: Name: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 2.767128 KDa

Sequence

String:

MYNGIGLPTP RGSGTNGYVQ RNLSLV

UniProtKB: Serine/arginine repetitive matrix protein 2

Macromolecule #24: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.610008 KDa

Sequence

String:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

Macromolecule #25: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.148711 KDa

Sequence

String:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #26: Intron-binding protein aquarius

• Macromolecule: Name: Intron-binding protein aquarius / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 171.502453 KDa

Sequence

String:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

UniProtKB: RNA helicase aquarius

Macromolecule #27: ATP-dependent RNA helicase DHX8

• Macromolecule: Name: ATP-dependent RNA helicase DHX8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 139.522312 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLI QTMRPPAKPS TSKDPVVKPK TEKEKLKELF PVLCQPDNPS VRTMLDEDDV KVAVDVLKEL EALMPSAAGQ E KQRDAEHR DRTKKKKRSR SRDRNRDRDR DRERNRDRDH KRRHRSRSRS RSRTRERNKV KSRYRSRSRS QSPPKDRKDR DK YGERNLD RWRDKHVDRP PPEEPTIGDI YNGKVTSIMQ FGCFVQLEGL RKRWEGLVHI SELRREGRVA NVADVVSKGQ RVK VKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKW EIKQM IAANVLSKEE FPDFDEETGI LPKVDDEEDE DLEIELVEEE PPFLRGHTKQ SMDMSPIKIV KNPDGSLSQA AMMQS ALAK ERRELKQAQR EAEMDSIPMG LNKHWVDPLP DAEGRQIAAN MRGIGMMPND IPEWKKHAFG GNKASYGKKT QMSILE QRE SLPIYKLKEQ LVQAVHDNQI LIVIGETGSG KTTQITQYLA EAGYTSRGKI GCTQPRRVAA MSVAKRVSEE FGCCLGQ EV GYTIRFEDCT SPETVIKYMT DGMLLRECLI DPDLTQYAII MLDEAHERTI HTDVLFGLLK KTVQKRQDMK LIVTSATL D AVKFSQYFYE APIFTIPGRT YPVEILYTKE PETDYLDASL ITVMQIHLTE PPGDILVFLT GQEEIDTACE ILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAG RAGRTGPGKC YRLYTERAYR DEMLTTNVPE IQRTNLASTV LSLKAMGIND LLSFDFMDAP PMETLITAME Q LYTLGALD DEGLLTRLGR RMAEFPLEPM LCKMLIMSVH LGCSEEMLTI VSMLSVQNVF YRPKDKQALA DQKKAKFHQT EG DHLTLLA VYNSWKNNKF SNPWCYENFI QARSLRRAQD IRKQMLGIMD RHKLDVVSCG KSTVRVQKAI CSGFFRNAAK KDP QEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEP LYNRY EEPNAWRISR AFRRR

UniProtKB: ATP-dependent RNA helicase DHX8

Macromolecule #28: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.69773 KDa

Sequence

String:

VKLTAELIEQ AAQYTNAVRD RELDLRGYKI PVIENLGATL DQFDAIDFSD NEIRKLDGFP LLRRLKTLLV NNNRICRIGE GLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK M FK

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #29: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.688563 KDa

Sequence

String:

IRPNHTIYIN NMNDKIKKEE LKRSLYALFS QFGHVVDIVA LKTMKMRGQA FVIFKELGSS TNALRQLQGF PFYGKPMRIQ YAKTDSDII SKM

UniProtKB: U2 small nuclear ribonucleoprotein B''

Macromolecule #30: NF-kappa-B-activating protein

• Macromolecule: Name: NF-kappa-B-activating protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.321766 KDa

Sequence

String:

KRIPRRGEIG LTSEEIASFE CSGYVMSGSR

UniProtKB: NF-kappa-B-activating protein

Macromolecule #31: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.551284 KDa

Sequence

String:

SSKMLQHIDY RMRCILQDGR IFIGTFKAFD KHMNLILCDC DEFRKIKPKN SKQAEREEKR VLGLVLLRGE NLVSMTVEGP PP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

Macromolecule #32: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 68.510844 KDa

Sequence

String:

MSATVVDAVN AAPLSGSKEM SLEEPKKMTR EDWRKKKELE EQRKLGNAPA EVDEEGKDIN PHIPQYISSV PWYIDPSKRP TLKHQRPQP EKQKQFSSSG EWYKRGVKEN SIITKYRKGA CENCGAMTHK KKDCFERPRR VGAKFTGTNI APDEHVQPQL M FDYDGKRD RWNGYNPEEH MKIVEEYAKV DLAKRTLKAQ KLQEELASGK LVEQANSPKH QWGEEEPNSQ MEKDHNSEDE DE DKYADDI DMPGQNFDSK RRITVRNLRI REDIAKYLRN LDPNSAYYDP KTRAMRENPY ANAGKNPDEV SYAGDNFVRY TGD TISMAQ TQLFAWEAYD KGSEVHLQAD PTKLELLYKS FKVKKEDFKE QQKESILEKY GGQEHLDAPP AELLLAQTED YVEY SRHGT VIKGQERAVA CSKYEEDVKI HNHTHIWGSY WKEGRWGYKC CHSFFKYSYC TGEAGKEIVN SEECIINEIT GEESV KKPQ TLMELHQEKL KEEKKKKKKK KKKHRKSSSD SDDEEKKHEK LKKALNAEEA RLLHVKETMQ IDERKRPYNS MYETRE PTE EEMEAYRMKR QRPDDPMASF LGQ

UniProtKB: Pre-mRNA-splicing factor SLU7

Macromolecule #33: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.460979 KDa

Sequence

String:

SIGVPIKVLH EAEGHIVTCE TNTGEVYRGK LIEAEDNMNC QMSNITVTYR DGRVAQLEQV YIRGSKIRFL ILPDMLKNAP MLKS

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #34: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.582185 KDa

Sequence

String:

QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV S

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #35: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.038415 KDa

Sequence

String:

PLNPKPFLNG LTGKPVMVKL KWGMEYKGYL VSVDGYMNMQ LANTEEYIDG ALSGHLGEVL IRCNNVLYIR GV

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #36: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.160629 KDa

Sequence

String:

AHPPELKKFM DKKLSLKLNG GRHVQGILRG FDPFMNLVID ECVEMATSGQ QNNIGMVVIR GNSIIMLEAL ERV

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #37: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.086731 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #38: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.055529 KDa

Sequence

String:

AIPPDSWQPP NVYLETSMGI IVLELYWKHA PKTCKNFAEL ARRGYYNGTK FHRIIKDFMI QGGDPTGTGR GGASIYGKQF EDELHPDLK FTGAGILAMA NAGPDTNGSQ FFVTLAPTQW LDGKHTIFGR VCQGIGMVNR VGMVETNSQD RPVDDVKIIK A YPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

Macromolecule #39: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #40: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 59.116055 KDa

Sequence

String:

ETGVHLDPAV KEVQYNPTYE TMFAPEFGPE NPFRTQQMAA PRNMLSGYAE PAHINDFMFE QQRRTFATYG YALDPSLDNH QVSAKYIGS VEEAEKNQGL TVFETGQKKT EKRKKFKEND ASNIDGFLGP WAKYVDEKDV AKPSEEEQKE LDEITAKRQK K GKQEEEKP GEEKTILHVK EMYDYQGRSY LHIPQDVGVN LRSTMPPEKC YLPKKQIHVW SGHTKGVSAV RLFPLSGHLL LS CSMDCKI KLWEVYGERR CLRTFIGHSK AVRDICFNTA GTQFLSAAYD RYLKLWDTET GQCISRFTNR KVPYCVKFNP DED KQNLFV AGMSDKKIVQ WDIRSGEIVQ EYDRHLGAVN TIVFVDENRR FVSTSDDKSL RVWEWDIPVD FKYIAEPSMH SMPA VTLSP NGKWLACQSM DNQILIFGAQ NRFRLNKKKI FKGHMVAGYA CQVDFSPDMS YVISGDGNGK LNIWDWKTTK LYSRF KAHD KVCIGAVWHP HETSKVITCG WDGLIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #41: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.16342 KDa

Sequence

String:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

UniProtKB: Pre-mRNA-splicing factor SPF27

Macromolecule #42: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 42 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 55.245547 KDa

Sequence

String:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #43: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.302371 KDa

Sequence

String:

LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE FFPTSNSLLH GTHVPSTEEI DRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RGTAV

UniProtKB: Pre-mRNA-splicing factor SYF2

Macromolecule #44: Replication stress response regulator SDE2

• Macromolecule: Name: Replication stress response regulator SDE2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 4.036538 KDa

Sequence

String:

EKTTNREACR DLSGRRLRDV NHEKAMAEWV KQQA

UniProtKB: Splicing regulator SDE2

Macromolecule #45: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #46: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 46 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #47: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 47 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #48: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 48 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #49: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 49 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #50: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 50 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.3 mg/mL

Buffer

pH: 7.9
Component:

Concentration	Name	Formula
20.0 mM	HEPES
100.0 mM		KCl
0.2 mM	EDTA

• Grid: Model: Quantifoil R2/2 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 7 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III; Details: 3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Number grids imaged: 2 / Number real images: 6200 / Average exposure time: 10.0 sec. / Average electron dose: 53.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 135000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 103860
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)