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Structure paper

TitleA human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
Journal, issue, pagesScience, Vol. 363, Issue 6428, Page 710-714, Year 2019
Publish dateFeb 15, 2019
AuthorsSebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai /
PubMed AbstractDuring exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a ...During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.
External linksScience / PubMed:30705154 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 5.9 Å
Structure data

4525

6qdv

EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
PDB-6qdv: Human post-catalytic P complex spliceosome
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-4526:
Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, overall map
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-4527:
Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, focused refinement on core
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-4528:
Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, focused refinement on core
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-4529:
Human post-catalytic spliceosome (P complex), focused refinement on Aquarius and SYF1
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-4530:
Human post-catalytic spliceosome (P complex), focused refinement on Brr2
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4532:
Human post-catalytic spliceosome (P complex), focused refinement on DHX8
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-4533:
Human post-catalytic spliceosome (P complex), focused refinement on Prp19
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4534:
Human post-catalytic spliceosome (P complex), focused refinement on U2 snRNP
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-4535:
Human post-catalytic spliceosome (P complex), focused refinement on U5 Sm
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-ZN:
Unknown entry

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

Source
  • homo sapiens (human)
  • human adenovirus 2
  • Human (human)
KeywordsSPLICING / spliceosome / RNA / complex

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