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- EMDB-3881: Sacbrood virus of honeybee empty particle -

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Basic information

Entry
Database: EMDB / ID: EMD-3881
TitleSacbrood virus of honeybee empty particle
Map data
Sample
  • Virus: Sacbrood virus
    • Protein or peptide: structural protein VP1Structure
    • Protein or peptide: structural protein VP2Structure
    • Protein or peptide: structural protein VP3Structure
    • Protein or peptide: minor capsid protein MiCP
Function / homology
Function and homology information


RNA-protein covalent cross-linking / host cell membrane / viral capsid / host cell cytoplasm / RNA helicase activity / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / structural molecule activity ...RNA-protein covalent cross-linking / host cell membrane / viral capsid / host cell cytoplasm / RNA helicase activity / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / structural molecule activity / proteolysis / RNA binding / ATP binding / membrane / cytoplasm
Similarity search - Function
Picornavirales 3C/3C-like protease domain / Picornavirales 3C/3C-like protease domain profile. / Peptidase C3A/C3B, picornaviral / 3C cysteine protease (picornain 3C) / Picornavirus capsid / picornavirus capsid protein / Helicase, superfamily 3, single-stranded RNA virus / Superfamily 3 helicase of positive ssRNA viruses domain profile. / Helicase, superfamily 3, single-stranded DNA/RNA virus / RNA helicase ...Picornavirales 3C/3C-like protease domain / Picornavirales 3C/3C-like protease domain profile. / Peptidase C3A/C3B, picornaviral / 3C cysteine protease (picornain 3C) / Picornavirus capsid / picornavirus capsid protein / Helicase, superfamily 3, single-stranded RNA virus / Superfamily 3 helicase of positive ssRNA viruses domain profile. / Helicase, superfamily 3, single-stranded DNA/RNA virus / RNA helicase / Picornavirus/Calicivirus coat protein / Viral coat protein subunit / RNA-directed RNA polymerase, C-terminal domain / Viral RNA-dependent RNA polymerase / Reverse transcriptase/Diguanylate cyclase domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / Peptidase S1, PA clan, chymotrypsin-like fold / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Genome polyprotein / Genome polyprotein / Genome polyprotein / Structural protein Vp1 / Genome polyprotein
Similarity search - Component
Biological speciesSacbrood virus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.87 Å
AuthorsPlevka P / Prochazkova M
Funding support Czech Republic, 2 items
OrganizationGrant numberCountry
European Research CouncilFP/2007-2013 (355855) Czech Republic
European Molecular Biology OrganizationIG-3041 Czech Republic
CitationJournal: Proc Natl Acad Sci U S A / Year: 2018
Title: Virion structure and genome delivery mechanism of sacbrood honeybee virus.
Authors: Michaela Procházková / Tibor Füzik / Karel Škubník / Jana Moravcová / Zorica Ubiparip / Antonín Přidal / Pavel Plevka /
Abstract: Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey ...Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey bees, the structure of its particles and mechanism of its genome delivery are unknown. Here we present the crystal structure of SBV virion and show that it contains 60 copies of a minor capsid protein (MiCP) attached to the virion surface. No similar MiCPs have been previously reported in any of the related viruses from the order Picornavirales. The location of the MiCP coding sequence within the SBV genome indicates that the MiCP evolved from a C-terminal extension of a major capsid protein by the introduction of a cleavage site for a virus protease. The exposure of SBV to acidic pH, which the virus likely encounters during cell entry, induces the formation of pores at threefold and fivefold axes of the capsid that are 7 Å and 12 Å in diameter, respectively. This is in contrast to vertebrate picornaviruses, in which the pores along twofold icosahedral symmetry axes are currently considered the most likely sites for genome release. SBV virions lack VP4 subunits that facilitate the genome delivery of many related dicistroviruses and picornaviruses. MiCP subunits induce liposome disruption in vitro, indicating that they are functional analogs of VP4 subunits and enable the virus genome to escape across the endosome membrane into the cell cytoplasm.
History
DepositionSep 19, 2017-
Header (metadata) releaseOct 18, 2017-
Map releaseJul 18, 2018-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6eiw
  • Surface level: 4.5
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6eiw
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3881.png

Downloads & links

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Map

FileDownload / File: emd_3881.map.gz / Format: CCP4 / Size: 620.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.063 Å
Density
Contour LevelBy EMDB: 3.3 / Movie #1: 4
Minimum - Maximum-7.9891124 - 22.075127
Average (Standard dev.)0.00000000255 (±1)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-273-273-273
Dimensions546546546
Spacing546546546
CellA=B=C: 580.398 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0631.0631.063
M x/y/z546546546
origin x/y/z0.0000.0000.000
length x/y/z580.398580.398580.398
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-273-273-273
NC/NR/NS546546546
D min/max/mean-7.98922.0750.000

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Supplemental data

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Supplemental map: emd 3881 additional.map

Fileemd_3881_additional.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Sacbrood virus

EntireName: Sacbrood virus
Components
  • Virus: Sacbrood virus
    • Protein or peptide: structural protein VP1Structure
    • Protein or peptide: structural protein VP2Structure
    • Protein or peptide: structural protein VP3Structure
    • Protein or peptide: minor capsid protein MiCP

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Supramolecule #1: Sacbrood virus

SupramoleculeName: Sacbrood virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 89463 / Sci species name: Sacbrood virus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes
Host (natural)Organism: Apis mellifera (honey bee)
Virus shellShell ID: 1 / Name: capsid / Diameter: 273.94 Å / T number (triangulation number): 3

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Macromolecule #1: structural protein VP1

MacromoleculeName: structural protein VP1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sacbrood virus
Molecular weightTheoretical: 26.1515 KDa
SequenceString: FSDGVTAMGF QSLDTQVSIK DILRRPVLLF NHVELDPDYT GFFIPIMPPS RMMQYKSGDK ETSFQRLIGR TPQAAIMNLF RFWRGSLRY TIIIHSTDGH PIYVTHVPHT GNRVYGLMKV NNLHEYTKVP IFGCGLTTEM IIPSVNPSIC VEVPFDTENN W AVTFDEDA ...String:
FSDGVTAMGF QSLDTQVSIK DILRRPVLLF NHVELDPDYT GFFIPIMPPS RMMQYKSGDK ETSFQRLIGR TPQAAIMNLF RFWRGSLRY TIIIHSTDGH PIYVTHVPHT GNRVYGLMKV NNLHEYTKVP IFGCGLTTEM IIPSVNPSIC VEVPFDTENN W AVTFDEDA QRNYSWRDKG DTVTGHLVVT PVVSVYMSVW VEAGDDFEVS NFYGPPSVKT NDWNYAFSDE H

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Macromolecule #2: structural protein VP2

MacromoleculeName: structural protein VP2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sacbrood virus
Molecular weightTheoretical: 22.563734 KDa
SequenceString: GDLVIASSEP TQQFRSLTNR WMPINSIRVT VNGKRNDLLA QYYIPEDFLS THAKCAPNTI PFETYVYGKY ELEMKFVANG NKFQCGKVI ISVKFDSYQA DNINTGFQAA LSRPHIMLDL STNNEGVLKI PFRYHRAFVR NQTHKTATAG VRPGKFASIY V QVLSPLQT ...String:
GDLVIASSEP TQQFRSLTNR WMPINSIRVT VNGKRNDLLA QYYIPEDFLS THAKCAPNTI PFETYVYGKY ELEMKFVANG NKFQCGKVI ISVKFDSYQA DNINTGFQAA LSRPHIMLDL STNNEGVLKI PFRYHRAFVR NQTHKTATAG VRPGKFASIY V QVLSPLQT GEGGANDMFI RPFYRYTRAE FAGMSYKVPL T

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Macromolecule #3: structural protein VP3

MacromoleculeName: structural protein VP3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sacbrood virus
Molecular weightTheoretical: 30.701373 KDa
SequenceString: DKPKDVSSIT IIPKPRLGFP HGKGKSDAVA MRVNPVALTS FQDVSAYPDE PRTTLDIARI WGLRSTFNWG SGDEHGKELF NTVLDPGLR FYDQDYEGQI TPMEYVTGLY NFWSGPIELR FDFVSNAFHT GTVIISAEYN RSSTNTDECQ SHSTYTKTFH L GEQKSVHF ...String:
DKPKDVSSIT IIPKPRLGFP HGKGKSDAVA MRVNPVALTS FQDVSAYPDE PRTTLDIARI WGLRSTFNWG SGDEHGKELF NTVLDPGLR FYDQDYEGQI TPMEYVTGLY NFWSGPIELR FDFVSNAFHT GTVIISAEYN RSSTNTDECQ SHSTYTKTFH L GEQKSVHF TVPYIYDTVV RRNTASAYLP VTDYDKVDNV SRAQAMGIRA ESKMRVKVRV VNVLRPVAST TSTIEVLVYM RG GKNYALH GLKQSTYWPS NSVVPIDSFP PDGYDP

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Macromolecule #4: minor capsid protein MiCP

MacromoleculeName: minor capsid protein MiCP / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sacbrood virus
Molecular weightTheoretical: 3.107398 KDa
SequenceString:
DNPHRFLPAN VSNRWNEYSS AYLPRV

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration15 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
137.0 mMNaClSodium chloridesodium chloride
2.7 mMKClpotassium chloride
10.0 mMNa2HPO4disodium phosphate
1.8 mMKH2PO4potassium phosphate
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: OTHER / Pretreatment - Pressure: 101.325 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK II
Detailsempty particle

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated magnification: 74325 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 75000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
DetailsPreliminar grid screening was performed manually on FEI Tecnai F20 (cryo stage).
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 2-16 / Number grids imaged: 1 / Average exposure time: 1.0 sec. / Average electron dose: 21.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 31804
Details: Full virions were selected together with empty particles from the same set of micrographs. Subsequent 2D classification sorted the particles of two types. Full and empty particles were ...Details: Full virions were selected together with empty particles from the same set of micrographs. Subsequent 2D classification sorted the particles of two types. Full and empty particles were further reconstructed separately.
CTF correctionSoftware - Name: Gctf / Software - details: Dr. Kai Zhang
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5cdc


Details: Deposited IAPV model low pass filtered to resolution 40 A.
Initial angle assignmentType: OTHER
Final 3D classificationNumber classes: 3 / Software - Name: RELION (ver. 2) / Details: re-classification after initial 3D refinement
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2) / Software - details: relion_refine_mpi
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.87 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2) / Number images used: 10303
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 99.36 / Target criteria: R-factor
Output model

PDB-6eiw:
Sacbrood virus of honeybee empty particle

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