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- EMDB-3656: Structure of the Bacillus subtilis hibernating 100S ribosome reve... -

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Basic information

Entry
Database: EMDB / ID: EMD-3656
TitleStructure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization.
Map dataCryo-EM reconstruction of the Bacillus subtilis 100s subcomplex (70s-30s)
Sample
  • Complex: Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization
    • RNA: x 3 types
    • Protein or peptide: x 48 types
Keywords100S / Bacillus subtilis / cryo-EM / Hibernation / HPF / RMF / rRNA / YvyD / translation
Function / homology
Function and homology information


negative regulation of translational elongation / positive regulation of rRNA processing / nucleoid / ribosomal small subunit binding / rRNA processing / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit ...negative regulation of translational elongation / positive regulation of rRNA processing / nucleoid / ribosomal small subunit binding / rRNA processing / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / DNA binding / RNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. ...Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / : / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L17 signature. / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S14/S29 / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein S15, bacterial-type / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein S12, bacterial-type
Similarity search - Domain/homology
Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 ...Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Ribosome hibernation promotion factor / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31
Similarity search - Component
Biological speciesBacillus subtilis subsp. subtilis str. 168 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsBeckert B / Abdelshahid M / Schaefer H / Steinchen W / Arenz S / Berninghausen O / Beckmann R / Bange G / Turgay K / Wilson DN
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research FoundationSPP-1879 Germany
CitationJournal: EMBO J / Year: 2017
Title: Structure of the hibernating 100S ribosome reveals the basis for 70S dimerization.
Authors: Bertrand Beckert / Maha Abdelshahid / Heinrich Schäfer / Wieland Steinchen / Stefan Arenz / Otto Berninghausen / Roland Beckmann / Gert Bange / Kürşad Turgay / Daniel N Wilson /
Abstract: Under stress conditions, such as nutrient deprivation, bacteria enter into a hibernation stage, which is characterized by the appearance of 100S ribosomal particles. In , dimerization of 70S ...Under stress conditions, such as nutrient deprivation, bacteria enter into a hibernation stage, which is characterized by the appearance of 100S ribosomal particles. In , dimerization of 70S ribosomes into 100S requires the action of the ribosome modulation factor (RMF) and the hibernation-promoting factor (HPF). Most other bacteria lack RMF and instead contain a long form HPF (LHPF), which is necessary and sufficient for 100S formation. While some structural information exists as to how RMF and HPF mediate formation of 100S (100S), structural insight into 100S formation by LHPF has so far been lacking. Here we present a cryo-EM structure of the hibernating 100S (100S), revealing that the C-terminal domain (CTD) of the LHPF occupies a site on the 30S platform distinct from RMF Moreover, unlike RMF, the HPF-CTD is directly involved in forming the dimer interface, thereby illustrating the divergent mechanisms by which 100S formation is mediated in the majority of bacteria that contain LHPF, compared to some γ-proteobacteria, such as .
History
DepositionMar 29, 2017-
Header (metadata) releaseJun 14, 2017-
Map releaseJun 14, 2017-
UpdateMay 15, 2024-
Current statusMay 15, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 11
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 11
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5njt
  • Surface level: 11
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3656.png

Downloads & links

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Map

FileDownload / File: emd_3656.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM reconstruction of the Bacillus subtilis 100s subcomplex (70s-30s)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 450 pix.
= 487.8 Å		1.08 Å/pix.
x 450 pix.
= 487.8 Å		1.08 Å/pix.
x 450 pix.
= 487.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.084 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 11.0 / Movie #1: 11
Minimum - Maximum-33.631385999999999 - 55.100189999999998
Average (Standard dev.)0.1278415 (±2.3855093)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 487.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0841.0841.084
M x/y/z450450450
origin x/y/z0.0000.0000.000
length x/y/z487.800487.800487.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS450450450
D min/max/mean-33.63155.1000.128

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Supplemental data

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Half map: Half Map 1 of the Bacillus subtilis 100s subcomplex (70s-30s)

Fileemd_3656_half_map_1.map
AnnotationHalf Map 1 of the Bacillus subtilis 100s subcomplex (70s-30s)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map 2 of the Bacillus subtilis 100s subcomplex (70s-30s)

Fileemd_3656_half_map_2.map
AnnotationHalf Map 2 of the Bacillus subtilis 100s subcomplex (70s-30s)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of the Bacillus subtilis hibernating 100S ribosome reve...

EntireName: Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization
Components
  • Complex: Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization
    • RNA: 16S ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L10
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 1
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: Ribosome hibernation promotion factor

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Supramolecule #1: Structure of the Bacillus subtilis hibernating 100S ribosome reve...

SupramoleculeName: Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

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Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 500.263219 KDa
SequenceString: GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC ...String:
GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC GGAUGGUUGU UUGAACCGCA UGGUUCAAAC AUAAAAGGUG GCUUCGGCUA CCACUUACAG AUGGACCCGC GG CGCAUUA GCUAGUUGGU GAGGUAACGG CUCACCAAGG CGACGAUGCG UAGCCGACCU GAGAGGGUGA UCGGCCACAC UGG GACUGA GACACGGCCC AGACUCCUAC GGGAGGCAGC AGUAGGGAAU CUUCCGCAAU GGACGAAAGU CUGACGGAGC AACG CCGCG UGAGUGAUGA AGGUUUUCGG AUCGUAAAGC UCUGUUGUUA GGGAAGAACA AGUGCCGUUC GAAUAGGGCG GUACC UUGA CGGUACCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAGCGUU GUCCGG AAU UAUUGGGCGU AAAGGGCUCG CAGGCGGUUU CUUAAGUCUG AUGUGAAAGC CCCCGGCUCA ACCGGGGAGG GUCAUUG GA AACUGGGGAA CUUGAGUGCA GAAGAGGAGA GUGGAAUUCC ACGUGUAGCG GUGAAAUGCG UAGAGAUGUG GAGGAACA C CAGUGGCGAA GGCGACUCUC UGGUCUGUAA CUGACGCUGA GGAGCGAAAG CGUGGGGAGC GAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCGCC CCUUAGUGCU GCAGCUAACG CAUUAAGCAC UCCGCCUGG GGAGUACGGU CGCAAGACUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU U AAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUCUGAC AAUCCUAGAG AUAGGACGUC CCCUUCGGGG GC AGAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUG AUCUUA GUUGCCAGCA UUCAGUUGGG CACUCUAAGG UGACUGCCGG UGACAAACCG GAGGAAGGUG GGGAUGACGU CAAA UCAUC AUGCCCCUUA UGACCUGGGC UACACACGUG CUACAAUGGA CAGAACAAAG GGCAGCGAAA CCGCGAGGUU AAGCC AAUC CCACAAAUCU GUUCUCAGUU CGGAUCGCAG UCUGCAACUC GACUGCGUGA AGCUGGAAUC GCUAGUAAUC GCGGAU CAG CAUGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACACCG CCCGUCACAC CACGAGAGUU UGUAACACCC GAAGUCG GU GAGGUAACCU UUUAGGAGCC AGCCGCCGAA GGUGGGACAG AUGAUUGGGG UGAAGUCGUA ACAAGGUAGC CGUAUCGG A AGGUGCGGCU GGAUCACCUC CUU

GENBANK: GENBANK: AL009126.3

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Macromolecule #21: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 21 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 947.975188 KDa
SequenceString: UUAAGUUAGA AAGGGCGCAC GGUGGAUGCC UUGGCACUAG GAGCCGAUGA AGGACGGGAC GAACACCGAU AUGCUUCGGG GAGCUGUAA GCAAGCUUUG AUCCGGAGAU UUCCGAAUGG GGAAACCCAC CACUCGUAAU GGAGUGGUAU CCAUAUCUGA A UUCAUAGG ...String:
UUAAGUUAGA AAGGGCGCAC GGUGGAUGCC UUGGCACUAG GAGCCGAUGA AGGACGGGAC GAACACCGAU AUGCUUCGGG GAGCUGUAA GCAAGCUUUG AUCCGGAGAU UUCCGAAUGG GGAAACCCAC CACUCGUAAU GGAGUGGUAU CCAUAUCUGA A UUCAUAGG AUAUGAGAAG GCAGACCCGG GGAACUGAAA CAUCUAAGUA CCCGGAGGAA GAGAAAGCAA AUGCGAUUCC CU GAGUAGC GGCGAGCGAA ACGGGAUCAG CCCAAACCAA GAGGCUUGCC UCUUGGGGUU GUAGGACACU CUGUACGGAG UUA CAAAGG AACGAGGUAG AUGAAGAGGU CUGGAAAGGC CCGCCAUAGG AGGUAACAGC CCUGUAGUCA AAACUUCGUU CUCU CCUGA GUGGAUCCUG AGUACGGCGG AACACGUGAA AUUCCGUCGG AAUCCGGGAG GACCAUCUCC CAAGGCUAAA UACUC CCUA GUGACCGAUA GUGAACCAGU ACCGUGAGGG AAAGGUGAAA AGCACCCCGG AAGGGGAGUG AAAGAGAUCC UGAAAC CGU GUGCCUACAA GUAGUCAGAG CCCGUUAACG GGUGAUGGCG UGCCUUUUGU AGAAUGAACC GGCGAGUUAC GAUCUCG UG CAAGGUUAAG CAGAAGAUGC GGAGCCGCAG CGAAAGCGAG UCUGAAUAGG GCGCAUGAGU ACGUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCAUGU CCAGGGUGAA GUUCAGGUAA CACUGAAUGG AGGCCCGAAC CCACGCACGU UGAAAAGUG CGGGGAUGAG GUGUGGGUAG GGGUGAAAUG CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGGGCUA GCCUCAAGG UAAGAGUCUU GGAGGUAGAG CACUGAUUGG ACUAGGGGCC CCUACCGGGU UACCGAAUUC AGUCAAACUC C GAAUGCCA AUGACUUAUC CUUGGGAGUC AGACUGCGAG UGAUAAGAUC CGUAGUCGAA AGGGAAACAG CCCAGACCGC CA GCUAAGG UCCCAAAGUA UACGUUAAGU GGAAAAGGAU GUGGAGUUGC UUAGACAACC AGGAUGUUGG CUUAGAAGCA GCC ACCAUU UAAAGAGUGC GUAAUAGCUC ACUGGUCGAG UGACUCUGCG CCGAAAAUGU ACCGGGGCUA AACGUAUCAC CGAA GCUGC GGACUGUUCU UCGAACAGUG GUAGGAGAGC GUUCUAAGGG CUGUGAAGCC AGACCGGAAG GACUGGUGGA GCGCU UAGA AGUGAGAAUG CCGGUAUGAG UAGCGAAAGA GGGGUGAGAA UCCCCUCCAC CGAAUGCCUA AGGUUUCCUG AGGAAG GCU CGUCCGCUCA GGGUUAGUCG GGACCUAAGC CGAGGCCGAA AGGCGUAGGC GAUGGACAAC AGGUUGAUAU UCCUGUA CC ACCUCCUCAC CAUUUGAGCA AUGGGGGGAC GCAGGAGGAU AGGGUAAGCG CGGUAUUGGA UAUCCGCGUC CAAGCAGU U AGGCUGGGAA AUAGGCAAAU CCGUUUCCCA UAAGCCUGAG CUGUGAUGGC GAGCGAAAUA UAGUAGCGAA GUUCCUGAU UCCACACUGC CAAGAAAAGC CUCUAGCGAG GUGAGAGGUG CCCGUACCGC AAACCGACAC AGGUAGGCGA GGAGAGAAUC CUAAGGUGA UCGAGAGAAC UCUCGUUAAG GAACUCGGCA AAAUGACCCC GUAACUUCGG GAGAAGGGGU GCUCUGUUAG G GUGCAAGC CCGAGAGAGC CGCAGUGAAU AGGCCCAGGC GACUGUUUAG CAAAAACACA GGUCUCUGCG AAGCCGUAAG GC GAAGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG CGCUUAGCGU AAGCGAAGGU GCGAAUUGAA GCC CCAGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCCGCACGA AAGG CGCAA CGAUCUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUUA UAGUACCUGU GAAGAUGCAG GUUACCCGCG ACAGG ACGG AAAGACCCCG UGGAGCUUUA CUGCAGCCUG AUAUUGAAUG UUGGUACAGC UUGUACAGGA UAGGUAGGAG CCUUGG AAA CCGGAGCGCC AGCUUCGGUG GAGGCAUCGG UGGGAUACUA CCCUGGCUGU AUUGACCUUC UAACCCGCCG CCCUUAU CG GGCGGGGAGA CAGUGUCAGG UGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCCC AAAGGUUC C CUCAGAAUGG UUGGAAAUCA UUCGCAGAGU GUAAAGGCAC AAGGGAGCUU GACUGCGAGA CCUACAAGUC GAGCAGGGA CGAAAGUCGG GCUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGCUACCCC GGGGAUAACA GGCUUAUCU CCCCCAAGAG UCCACAUCGA CGGGGAGGUU UGGCACCUCG AUGUCGGCUC AUCGCAUCCU GGGGCUGUAG U CGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC CU AUCCGUC GCGGGCGCAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGACCGGG AUGGACGCAC CGCUGGUGUA CCA GUUGUU CUGCCAAGGG CAUCGCUGGG UAGCUAUGUG CGGACGGGAU AAGUGCUGAA AGCAUCUAAG CAUGAAGCCC CCCU CAAGA UGAGAUUUCC CAUUCCGCAA GGAAGUAAGA UCCCUGAAAG AUGAUCAGGU UGAUAGGUCU GAGGUGGAAG UGUGG CGAC ACAUGGAGCU GACAGAUACU AAUCGAUCGA GGACUUAAC

GENBANK: GENBANK: D26185.1

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Macromolecule #22: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 36.15752 KDa
SequenceString:
UGGUGGCGAU AGCGAAGAGG UCACACCCGU UCCCAUACCG AACACGGAAG UUAAGCUCUU CAGCGCCGAU GGUAGUCGGG GGUUUCCCC CUGUGAGAGU AGGACGCCGC CAA

GENBANK: GENBANK: CP010052.1

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Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 25.730895 KDa
SequenceString: MKQLLEAGVH FGHQTRRWNP KMKRYIFTER NGIYIIDLQK TVKKVEEAYN FTKNLAAEGG KILFVGTKKQ AQDSVKEEAQ RSGMYYVNQ RWLGGTLTNF ETIQKRIKRL KDIEKMQENG TFDVLPKKEV VQLKKELERL EKFLGGIKDM KDLPDALFII D PRKERIAV ...String:
MKQLLEAGVH FGHQTRRWNP KMKRYIFTER NGIYIIDLQK TVKKVEEAYN FTKNLAAEGG KILFVGTKKQ AQDSVKEEAQ RSGMYYVNQ RWLGGTLTNF ETIQKRIKRL KDIEKMQENG TFDVLPKKEV VQLKKELERL EKFLGGIKDM KDLPDALFII D PRKERIAV AEARKLNIPI IGIVDTNCDP DEIDVVIPAN DDAIRAVKLL TSKMADAILE AKQGEE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 23.488898 KDa
SequenceString: GQKVNPVGLR IGVIRDWESK WYAGKDYADF LHEDLKIREY ISKRLSDASV SKVEIERAAN RVNITIHTAK PGMVIGKGGS EVEALRKAL NSLTGKRVHI NILEIKRADL DAQLVADNIA RQLENRVSFR RAQKQQIQRT MRAGAQGVKT MVSGRLGGAD I ARSEYYSE ...String:
GQKVNPVGLR IGVIRDWESK WYAGKDYADF LHEDLKIREY ISKRLSDASV SKVEIERAAN RVNITIHTAK PGMVIGKGGS EVEALRKAL NSLTGKRVHI NILEIKRADL DAQLVADNIA RQLENRVSFR RAQKQQIQRT MRAGAQGVKT MVSGRLGGAD I ARSEYYSE GTVPLHTLRA DIDYATSEAD TTYGKLGVKV WIYRGEVLPT KK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 22.743074 KDa
SequenceString: ARYTGPSWKL SRRLGISLSG TGKELEKRPY APGPHGPGQR KKLSEYGLQL QEKQKLRHMY GVNERQFRTL FDKAGKLAGK HGENFMILL DSRLDNVVYK LGLARTRRQA RQLVNHGHIL VDGSRVDIPS YLVKPGQTIG VREKSRNLSI IKESVEVNNF V PEYLTFDA ...String:
ARYTGPSWKL SRRLGISLSG TGKELEKRPY APGPHGPGQR KKLSEYGLQL QEKQKLRHMY GVNERQFRTL FDKAGKLAGK HGENFMILL DSRLDNVVYK LGLARTRRQA RQLVNHGHIL VDGSRVDIPS YLVKPGQTIG VREKSRNLSI IKESVEVNNF V PEYLTFDA EKLEGTFTRL PERSELAPEI NEALIVEFYS R

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 17.51943 KDa
SequenceString:
RRIDPSKLEL EERLVTVNRV AKVVKGGRRF RFAALVVVGD KNGHVGFGTG KAQEVPEAIR KAVEDAKKNL IEVPMVGTTI PHEIIGRFG AGNILLKPAS EGTGVIAGGP VRAVLELAGV ADILSKSLGS NTPINMIRAT LQGLSELKRA EDVAKLRGKS V EELLG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 11.140548 KDa
SequenceString:
MRKYEVMYII RPNIDEESKK AVIERFNNVL TSNGAEITGT KDWGKRRLAY EINDFRDGFY QIVNVQSDAA AVQEFDRLAK ISDDIIRHI VVKEEE

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 17.530369 KDa
SequenceString:
KGPVAKRDVL PDPIYNSKLV SRLINKMMID GKKGKSQTIL YKSFDIIKER TGNDAMEVFE QALKNIMPVL EVKARRVGGA NYQVPVEVR PERRTTLGLR WLVNYARLRG EKTMEERLAN EILDAANNTG AAVKKREDTH KMAEANKAFA HYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 14.770229 KDa
SequenceString:
VMTDPIADML TRIRNANMVR HEKLEIPASK LKREIAEILK REGFIRDVEF VEDSKQGIIR VFLKYGQNNE RVITGLKRIS KPGLRVYAK SNEVPRVLNG LGIAIISTSQ GVLTDKEARA KQAGGEVLAY VW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 14.335504 KDa
SequenceString:
MAQVQYYGTG RRKSSVARVR LVPGEGRIVV NNREISEHIP SAALIEDIKQ PLTLTETAGT YDVLVNVHGG GLSGQAGAIR HGIARALLE ADPEYRTTLK RAGLLTRDAR MKERKKYGLK GARRAPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 11.687661 KDa
SequenceString:
MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSVYT ILRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDALMR LDLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 12.476212 KDa
SequenceString:
RRVKKNIESG IAHIRSTFNN TIVTITDTHG NAISWSSAGA LGFRGSRKST PFAAQMAAET AAKGSIEHGL KTLEVTVKGP GSGREAAIR ALQAAGLEVT AIRDVTPVPH NGCRPPKRR

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 15.117538 KDa
SequenceString:
PTINQLIRKG RVSKVENSKS PALNKGYNSF KKEHTNVSSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLT NGIEVTAYIP GIGHNLQEH SVVLIRGGRV KDLPGVRYHI VRGALDTAGV ENRAQGRSKY GTKKPKAK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 12.599545 KDa
SequenceString:
ARIAGVDIPR DKRVVISLTY IFGIGRTTAQ QVLKEAGVSE DTRVRDLTEE ELGKIRDIID KLKVEGDLRR EVSLNIKRLI EIGSYRGIR HRRGLPVRGQ NSKNNARTRK GP

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #14: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 7.132607 KDa
SequenceString:
AKKSMIAKQQ RTPKFKVQEY TRCERCGRPH SVIRKFKLCR ICFRELAYKG QIPGVKKASW

UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 10.466028 KDa
SequenceString:
AITQERKNQL INEFKTHESD TGSPEVQIAI LTDSINNLNE HLRTHKKDHH SRRGLLKMVG KRRNLLTYLR NKDVTRYREL INKLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 10.022637 KDa
SequenceString:
AVKIRLKRMG AKKSPFYRIV VADSRSPRDG RFIETVGTYN PVAKPAEVKI DEELALKWLQ TGAKPSDTVR NLFSSQGIME KFHNAKQGK

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 10.089784 KDa
SequenceString:
SERNQRKVYQ GRVVSDKMDK TITVVVETYK KHTLYGKRVK YSKKFKAHDE NNQAKIGDIV KIMETRPLSA TKRFRLVEVV EEAVII

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 8.245746 KDa
SequenceString:
RAKRRKVCYF TSNGITHIDY KDVDLLKKFV SERGKILPRR VTGTNAKYQR KLTAAIKRAR QMALLPYVSG E

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 9.185659 KDa
SequenceString:
SLKKGPFVDG HLMTKIEKLN ETDKKQVVKT WSRRSTIFPQ FIGHTIAVYD GRKHVPVFIS EDMVGHKLGE FAPTRTYKGH

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 9.393906 KDa
SequenceString:
NIKSAIKRTK TNNERRVHNA TIKSAMRTAI KQVEASVANN EADKAKTALT EAAKRIDKAV KTGLVHKNTA ARYKSRLAKK VNGLSA

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #23: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 30.07475 KDa
SequenceString: AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG ...String:
AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG KYVLVRLNSG EVRMILSACR ASIGQVGNEQ HELINIGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRKSPMS PWGKPTLGFK TRKKKNKSDK FIVRRRKN

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #24: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 22.462973 KDa
SequenceString: TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI ...String:
TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI TVQNLEIVKV DAERNLLLIK GNVPGAKKSL ITVKSAVKS

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #25: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 22.222676 KDa
SequenceString: PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN ...String:
PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN IPGVTVVEAN GINVLDVVNH EKLLITKAAV EKVEEVL

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #26: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 20.046371 KDa
SequenceString:
NRLKEKYNKE IAPALMTKFN YDSVMQVPKI EKIVINMGVG DAVQNAKAID SAVEELTFIA GQKPVVTRAK KSIAGFRLRE GMPIGAKVT LRGERMYDFL DKLISVSLPR VRDFRGVSKK SFDGRGNYTL GIKEQLIFPE IDYDKVTKVR GMDIVIVTTA N TDEEAREL LTQVGMPFQK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #27: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 19.124855 KDa
SequenceString:
SRVGKKLLEI PSDVTVTLND NNTVAVKGPK GELTRTFHPD MEIKVEDNVL TVARPSDQKE HRALHGTTRS LLGNMVEGVS KGFERGLEL VGVGYRASKS GNKLVLNVGY SHPVEIVPEE GIEIEVPSQT KVVVKGTDKE RVGAIAANIR AVRSPEPYKG K GIRYEGEV VRRKEGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #28: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 13.607533 KDa
SequenceString:
AIETKKVVVE EIASKLKESK STIIVDYRGL NVSEVTELRK QLREANVEFK VYKNTMTRRA VEQAELNGLN DFLTGPNAIA FSTEDVVAP AKVLNDFAKN HEALEIKAGV IEGKVSTVEE VKAL

UniProtKB: Large ribosomal subunit protein uL10

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Macromolecule #29: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 16.01761 KDa
SequenceString:
TPMANASTIE RKWLVVDAAG KTLGRLSSEV AAILRGKHKP TYTPHVDTGD HVIIINAEKI ELTGKKLTDK IYYRHTQHPG GLKSRTALE MRTNYPEKML ELAIKGMLPK GSLGRQMFKK LNVYRGSEHP HEAQKPEVYE LRG

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #30: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 13.175288 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #31: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 15.410694 KDa
SequenceString:
MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #32: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 15.619512 KDa
SequenceString:
MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKREEIG

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #33: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 13.643609 KDa
SequenceString:
SYRKLGRTSA QRKAMLRDLT TDLIINERIE TTETRAKELR SVVEKMITLG KRGDLHARRQ AAAYIRNEVA NEENNQDALQ KLFSDIATR YEERQGGYTR IMKLGPRRGD GAPMAIIELV

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #34: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 12.993829 KDa
SequenceString:
MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #35: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 13.285655 KDa
SequenceString:
QKLIEDITKE QLRTDLPAFR PGDTLRVHVK VVEGNRERIQ IFEGVVIKRR GGGISETFTV RKISYGVGVE RTFPVHTPKI AKIEVVRYG KVRRAKLYYL RELRGKAARI KEIRR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #36: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 13.408813 KDa
SequenceString:
PRVKGGTVTR KRRKKVLKLA KGYFGSKHTL YKVANQQVMK SGNYAFRDRR QKKRDFRKLW ITRINAAARM NGLSYSRLMH GLKLSGIEV NRKMLADLAV NDLTAFNQLA DAAKAQLN

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #37: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 11.164886 KDa
SequenceString:
YAIIKTGGKQ IKVEEGQTVY IEKLAAEAGE TVTFEDVLFV GGDNVKVGNP TVEGATVTAK VEKQGRAKKI TVFRYKPKKN VHKKQGHRQ PYTKVTIEKI NA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #38: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 12.035066 KDa
SequenceString:
QAKAVARTVR IAPRKARLVM DLIRGKQVGE AVSILNLTPR AASPIIEKVL KSAIANAEHN YEMDANNLVI SQAFVDEGPT LKRFRPRAM GRASQINKRT SHITIVVSEK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #39: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 10.778622 KDa
SequenceString:
MKDPRDVLKR PVITERSADL MTEKKYTFEV DVRANKTEVK DAVESIFGVK VDKVNIMNYK GKSKRVGRYT GMTSRRRKAI VKLTADSKE IEIF

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #40: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 10.790657 KDa
SequenceString:
HVKKGDKVMV ISGKDKGKQG TILAAFPKKD RVLVEGVNMV KKHSKPTQAN PQGGISNQEA PIHVSNVMPL DPKTGEVTRV GYKVEDGKK VRVAKKSGQV L

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #41: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 8.956143 KDa
SequenceString:
SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSVY PV

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #42: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 6.184386 KDa
SequenceString:
AVPFRRTSKM KKRLRRTHFK LNVPGMTECP SCGEMKLSHR VCKACGSYNG KDIN

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #43: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 5.786696 KDa
SequenceString:
MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRET

UniProtKB: Large ribosomal subunit protein bL33A

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Macromolecule #44: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 5.271332 KDa
SequenceString:
MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #45: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 7.320752 KDa
SequenceString:
PKMKTHRGSA KRFKKTGSGK LKRSHAYTSH LFANKSQKQK RKLRKSAVVS AGDFKRIKQM LANI

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #46: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 4.187226 KDa
SequenceString:
KVRPSVKPIC EKCKVIRRKG KVMVICENPK HKQKQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #47: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 6.367546 KDa
SequenceString:
RKCVITGKKT TAGNNRSHAM NASKRTWGAN LQKVRILVNG KPKKVYVSAR ALKSGKVE

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #48: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 7.598849 KDa
SequenceString:
MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAAN

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #49: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 6.519599 KDa
SequenceString:
AKLEITLKRS VIGRPEDQRV TVRTLGLKKT NQTVVHEDNA AIRGMINKVS HLVSVKEQ

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #50: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 7.216244 KDa
SequenceString:
MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRQKFA SADGRVDRFN KKYG

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #51: Ribosome hibernation promotion factor

MacromoleculeName: Ribosome hibernation promotion factor / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 12.355955 KDa
SequenceString:
MNYNIRGENI EVTPALKDHV ERKIGKLERY FDHSVDADVN VNLKFYNDKE SKVEVTIPMT DLALRSEVHN EDMYNAIDLA TNKLERQIR KHKTKVNRKF REQES

UniProtKB: Ribosome hibernation promotion factor

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationNameFormula
50.0 mMHepes-KOH
100.0 mMKOAc
25.0 mMMg(OAc)2
GridModel: Quantifoil R3/3 / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE
Details4 OD260/ml ml Bs100S sample were applied to 2 nm pre-coated Quantifoil R3/3 holey carbon supported grids and vitrified using Vitrobot Mark IV (FEI Company)

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: COUNTING / Average electron dose: 2.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 253905
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3jw9

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: FREALIGN (ver. 9.11)
Details: 30S-70S_subcomplex primary map: 70S-30S_Masked_embf41.mrc
Number images used: 24546
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: FREALIGN (ver. 9.11)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: FREALIGN (ver. 9.11)
Final 3D classificationSoftware - Name: FREALIGN (ver. 9.11)

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