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基本情報
登録情報 | データベース: EMDB / ID: EMD-3461 | |||||||||
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タイトル | Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae | |||||||||
![]() | Native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae | |||||||||
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機能・相同性 | ![]() cytoplasmic translational elongation through polyproline stretches / positive regulation of cytoplasmic translational elongation through polyproline stretches / Hypusine synthesis from eIF5A-lysine / CAT tailing / translational frameshifting / mRNA decay by 3' to 5' exoribonuclease / protein-DNA complex assembly / Ski complex / cytoplasmic translational elongation / positive regulation of translational termination ...cytoplasmic translational elongation through polyproline stretches / positive regulation of cytoplasmic translational elongation through polyproline stretches / Hypusine synthesis from eIF5A-lysine / CAT tailing / translational frameshifting / mRNA decay by 3' to 5' exoribonuclease / protein-DNA complex assembly / Ski complex / cytoplasmic translational elongation / positive regulation of translational termination / nuclear-transcribed mRNA catabolic process, non-stop decay / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / cytoplasmic translational termination / positive regulation of translational elongation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / reciprocal meiotic recombination / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / nuclear chromosome / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / mRNA catabolic process / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of translational frameshifting / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / nuclear-transcribed mRNA catabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / positive regulation of translational initiation / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / 90S preribosome / positive regulation of protein kinase activity / Ub-specific processing proteases / translation elongation factor activity / ribosomal subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational termination / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / translation initiation factor activity / cellular response to amino acid starvation / ribosome assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / protein kinase C binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / translational initiation / small-subunit processome / macroautophagy / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / cytoplasmic stress granule / rRNA processing / regulation of translation / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / protein-containing complex assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to virus / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.8 Å | |||||||||
![]() | Schmidt C / Kowalinski E / Shanmuganathan V / Defenouillere Q / Braunger K / Heuer A / Pech M / Namane A / Berninghausen O / Fromont-Racine M ...Schmidt C / Kowalinski E / Shanmuganathan V / Defenouillere Q / Braunger K / Heuer A / Pech M / Namane A / Berninghausen O / Fromont-Racine M / Jacquier A / Conti E / Becker T / Beckmann R | |||||||||
![]() | ![]() タイトル: The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex. 著者: Christian Schmidt / Eva Kowalinski / Vivekanandan Shanmuganathan / Quentin Defenouillère / Katharina Braunger / André Heuer / Markus Pech / Abdelkader Namane / Otto Berninghausen / ...著者: Christian Schmidt / Eva Kowalinski / Vivekanandan Shanmuganathan / Quentin Defenouillère / Katharina Braunger / André Heuer / Markus Pech / Abdelkader Namane / Otto Berninghausen / Micheline Fromont-Racine / Alain Jacquier / Elena Conti / Thomas Becker / Roland Beckmann / ![]() ![]() 要旨: Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski ...Ski2-Ski3-Ski8 (Ski) is a helicase complex functioning with the RNA-degrading exosome to mediate the 3'-5' messenger RNA (mRNA) decay in turnover and quality-control pathways. We report that the Ski complex directly associates with 80S ribosomes presenting a short mRNA 3' overhang. We determined the structure of an endogenous ribosome-Ski complex using cryo-electron microscopy (EM) with a local resolution of the Ski complex ranging from 4 angstroms (Å) in the core to about 10 Å for intrinsically flexible regions. Ribosome binding displaces the autoinhibitory domain of the Ski2 helicase, positioning it in an open conformation near the ribosomal mRNA entry tunnel. We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation. | |||||||||
履歴 |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 381.9 MB | ![]() | |
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画像 | ![]() | 38.7 KB | ||
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「今月の分子」の関連する項目 |
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ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.084 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
+全体 : Native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae
+超分子 #1: Native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae
+分子 #1: 18S ribosomal RNA
+分子 #35: mRNA
+分子 #36: A-site tRNA
+分子 #37: P-site tRNA
+分子 #83: 25S ribosomal RNA
+分子 #84: 5S ribosomal RNA
+分子 #85: 5.8S ribosomal RNA
+分子 #2: 40S ribosomal protein S3
+分子 #3: 40S ribosomal protein S5
+分子 #4: 40S ribosomal protein S10-A
+分子 #5: 40S ribosomal protein S12
+分子 #6: 40S ribosomal protein S15
+分子 #7: 40S ribosomal protein S16-A
+分子 #8: 40S ribosomal protein S17-B
+分子 #9: 40S ribosomal protein S18-A
+分子 #10: 40S ribosomal protein S19-A
+分子 #11: 40S ribosomal protein S20
+分子 #12: 40S ribosomal protein S25-A
+分子 #13: 40S ribosomal protein S28-B
+分子 #14: 40S ribosomal protein S29-A
+分子 #15: Ubiquitin-40S ribosomal protein S31
+分子 #16: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #17: 40S ribosomal protein S0-A
+分子 #18: 40S ribosomal protein S1-A
+分子 #19: 40S ribosomal protein S2
+分子 #20: 40S ribosomal protein S4-A
+分子 #21: 40S ribosomal protein S6-A
+分子 #22: 40S ribosomal protein S7-A
+分子 #23: 40S ribosomal protein S8-A
+分子 #24: 40S ribosomal protein S9-A
+分子 #25: 40S ribosomal protein S11-A
+分子 #26: 40S ribosomal protein S13
+分子 #27: 40S ribosomal protein S14-A
+分子 #28: 40S ribosomal protein S21-A
+分子 #29: 40S ribosomal protein S22-A
+分子 #30: 40S ribosomal protein S23-A
+分子 #31: 40S ribosomal protein S24-A
+分子 #32: 40S ribosomal protein S26-A
+分子 #33: 40S ribosomal protein S27-A
+分子 #34: 40S ribosomal protein S30-A
+分子 #38: Antiviral helicase SKI2
+分子 #39: Superkiller protein 3
+分子 #40: Antiviral protein SKI8
+分子 #41: 60S ribosomal protein L8-A
+分子 #42: 60S ribosomal protein L23-A
+分子 #43: 60S ribosomal protein L36-A
+分子 #44: 60S ribosomal protein L9-A
+分子 #45: 60S ribosomal protein L24-A
+分子 #46: 60S ribosomal protein L37-A
+分子 #47: 60S ribosomal protein L11-A
+分子 #48: 60S ribosomal protein L25
+分子 #49: 60S ribosomal protein L38
+分子 #50: 60S ribosomal protein L13-A
+分子 #51: 60S ribosomal protein L26-A
+分子 #52: 60S ribosomal protein L39
+分子 #53: 60S ribosomal protein L14-A
+分子 #54: 60S ribosomal protein L27-A
+分子 #55: Ubiquitin-60S ribosomal protein L40
+分子 #56: 60S ribosomal protein L42-A
+分子 #57: 60S ribosomal protein L15-A
+分子 #58: 60S ribosomal protein L28
+分子 #59: 60S ribosomal protein L41-A
+分子 #60: 60S ribosomal protein L43-A
+分子 #61: 60S ribosomal protein L16-A
+分子 #62: 60S ribosomal protein L29
+分子 #63: 60S ribosomal protein L2-A
+分子 #64: 60S ribosomal protein L17-A
+分子 #65: 60S ribosomal protein L30
+分子 #66: uL1
+分子 #67: 60S ribosomal protein L3
+分子 #68: 60S ribosomal protein L18-A
+分子 #69: 60S ribosomal protein L31-A
+分子 #70: 60S ribosomal protein L10
+分子 #71: 60S ribosomal protein L4-A
+分子 #72: 60S ribosomal protein L19-A
+分子 #73: 60S ribosomal protein L32
+分子 #74: 60S ribosomal protein L20-A
+分子 #75: 60S ribosomal protein L5
+分子 #76: 60S ribosomal protein L21-A
+分子 #77: 60S ribosomal protein L33-A
+分子 #78: 60S ribosomal protein L22-A
+分子 #79: 60S ribosomal protein L6-A
+分子 #80: 60S ribosomal protein L34-A
+分子 #81: 60S ribosomal protein L7-A
+分子 #82: 60S ribosomal protein L35-A
+分子 #86: Eukaryotic translation initiation factor 5A-1
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 構成要素:
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グリッド | モデル: Quantifoil R3/3 / 材質: COPPER/PALLADIUM / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 2.0 nm / 前処理 - タイプ: GLOW DISCHARGE | |||||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 278 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) デジタル化 - 画像ごとのフレーム数: 1-10 / 撮影したグリッド数: 2 / 平均電子線量: 2.8 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |