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- EMDB-21427: Single Particle Cryo-EM Structure of the Natively Isolated Sec61,... -

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Basic information

Entry
Database: EMDB / ID: EMD-21427
TitleSingle Particle Cryo-EM Structure of the Natively Isolated Sec61, TMCO1, Nicalin, TMEM147, and CCDC47 Containing Ribosome-Translocon Complex
Map data
SampleNative complex of a translating ribosome and a novel translocon containing the Sec61 complex, TMEM147, NOMO, Nicalin, CCDC47, and TMCO1.
  • 60S SubunitEukaryotic large ribosomal subunit (60S)
  • 40S Subunit
  • Translocon
  • Sec61 Complex
  • TMCO1
  • NOMO-Nicalin-TMEM147 Complex
  • CCDC47
  • (60S acidic ribosomal protein ...) x 3
  • (60S ribosomal protein ...) x 40
  • (40S ribosomal protein ...) x 31
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsMcGilvray PT / Anghel SA / Sundaram A / Trnka MJ / Zhong F / Hu H / Burlingame AL / Keenan RJ
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM130051-01 United States
CitationJournal: Elife / Year: 2020
Title: An ER translocon for multi-pass membrane protein biogenesis.
Authors: Philip T McGilvray / S Andrei Anghel / Arunkumar Sundaram / Frank Zhong / Michael J Trnka / James R Fuller / Hong Hu / Alma L Burlingame / Robert J Keenan /
Abstract: Membrane proteins with multiple transmembrane domains play critical roles in cell physiology, but little is known about the machinery coordinating their biogenesis at the endoplasmic reticulum. Here ...Membrane proteins with multiple transmembrane domains play critical roles in cell physiology, but little is known about the machinery coordinating their biogenesis at the endoplasmic reticulum. Here we describe a ~ 360 kDa ribosome-associated complex comprising the core Sec61 channel and five accessory factors: TMCO1, CCDC47 and the Nicalin-TMEM147-NOMO complex. Cryo-electron microscopy reveals a large assembly at the ribosome exit tunnel organized around a central membrane cavity. Similar to protein-conducting channels that facilitate movement of transmembrane segments, cytosolic and luminal funnels in TMCO1 and TMEM147, respectively, suggest routes into the central membrane cavity. High-throughput mRNA sequencing shows selective translocon engagement with hundreds of different multi-pass membrane proteins. Consistent with a role in multi-pass membrane protein biogenesis, cells lacking different accessory components show reduced levels of one such client, the glutamate transporter EAAT1. These results identify a new human translocon and provide a molecular framework for understanding its role in multi-pass membrane protein biogenesis.
History
DepositionFeb 21, 2020-
Header (metadata) releaseMar 25, 2020-
Map releaseSep 2, 2020-
UpdateSep 2, 2020-
Current statusSep 2, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0073
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0073
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_21427.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.36 Å/pix.
x 500 pix.
= 680. Å
1.36 Å/pix.
x 500 pix.
= 680. Å
1.36 Å/pix.
x 500 pix.
= 680. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.36 Å
Density
Contour LevelBy AUTHOR: 0.0073 / Movie #1: 0.0073
Minimum - Maximum-0.014456131 - 0.04746323
Average (Standard dev.)0.00015776935 (±0.0019139652)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin00500
Dimensions500500500
Spacing500500500
CellA=B=C: 680.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.361.361.36
M x/y/z500500500
origin x/y/z0.0000.0000.000
length x/y/z680.000680.000680.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS00500
NC/NR/NS500500500
D min/max/mean-0.0140.0470.000

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Supplemental data

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Half map: #2

Fileemd_21427_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #1

Fileemd_21427_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

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Sample components

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Entire Native complex of a translating ribosome and a novel translocon c...

EntireName: Native complex of a translating ribosome and a novel translocon containing the Sec61 complex, TMEM147, NOMO, Nicalin, CCDC47, and TMCO1.
Number of Components: 82

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Component #1: protein, Native complex of a translating ribosome and a novel tra...

ProteinName: Native complex of a translating ribosome and a novel translocon containing the Sec61 complex, TMEM147, NOMO, Nicalin, CCDC47, and TMCO1.
Recombinant expression: No
MassTheoretical: 56 kDa
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #2: protein, 60S Subunit

ProteinName: 60S SubunitEukaryotic large ribosomal subunit (60S)
Details: 60S ribosomal protein L9 60S ribosomal protein L8 60S ribosomal protein L7a 60S ribosomal protein L7 60S ribosomal protein L6 60S ribosomal protein L5 60S ribosomal protein L4 60S ribosomal ...Details: 60S ribosomal protein L9 60S ribosomal protein L8 60S ribosomal protein L7a 60S ribosomal protein L7 60S ribosomal protein L6 60S ribosomal protein L5 60S ribosomal protein L4 60S ribosomal protein L39 60S ribosomal protein L38 60S ribosomal protein L37a 60S ribosomal protein L36a 60S ribosomal protein L36 60S ribosomal protein L35a 60S ribosomal protein L35 60S ribosomal protein L34 60S ribosomal protein L32 60S ribosomal protein L31 60S ribosomal protein L30 60S ribosomal protein L3 60S ribosomal protein L28 60S ribosomal protein L27a 60S ribosomal protein L27 60S ribosomal protein L26 60S ribosomal protein L24 60S ribosomal protein L23a 60S ribosomal protein L23 60S ribosomal protein L22-like 1 60S ribosomal protein L22 60S ribosomal protein L21 60S ribosomal protein L19 60S ribosomal protein L18a 60S ribosomal protein L18 60S ribosomal protein L17 60S ribosomal protein L15 60S ribosomal protein L14 60S ribosomal protein L13a 60S ribosomal protein L13 60S ribosomal protein L12 60S ribosomal protein L11 60S ribosomal protein L10a 60S ribosomal protein L10 60S ribosomal export protein NMD3 60S acidic ribosomal protein P2 60S acidic ribosomal protein P1 60S acidic ribosomal protein P0-like 60S acidic ribosomal protein P0 Ubiquitin-60S ribosomal protein L40Ribosome
Recombinant expression: No
MassTheoretical: 2.42 MDa
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #3: protein, 40S Subunit

ProteinName: 40S Subunit
Details: 40S ribosomal protein SA 40S ribosomal protein S9 40S ribosomal protein S8 40S ribosomal protein S7 40S ribosomal protein S6 40S ribosomal protein S5 40S ribosomal protein S4, X isoform 40S ...Details: 40S ribosomal protein SA 40S ribosomal protein S9 40S ribosomal protein S8 40S ribosomal protein S7 40S ribosomal protein S6 40S ribosomal protein S5 40S ribosomal protein S4, X isoform 40S ribosomal protein S3a 40S ribosomal protein S30 40S ribosomal protein S3 40S ribosomal protein S29 40S ribosomal protein S28 40S ribosomal protein S27-like 40S ribosomal protein S27 40S ribosomal protein S26 40S ribosomal protein S25 40S ribosomal protein S24 40S ribosomal protein S23 40S ribosomal protein S21 40S ribosomal protein S20 40S ribosomal protein S2 40S ribosomal protein S19 40S ribosomal protein S18 40S ribosomal protein S17 40S ribosomal protein S16 40S ribosomal protein S15a 40S ribosomal protein S15 40S ribosomal protein S14 40S ribosomal protein S13 40S ribosomal protein S12 40S ribosomal protein S11 40S ribosomal protein S10 Ubiquitin-40S ribosomal protein S27aRibosome
Recombinant expression: No
MassTheoretical: 1.12 MDa
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #4: protein, Translocon

ProteinName: Translocon / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #5: protein, Sec61 Complex

ProteinName: Sec61 Complex / Details: Sec61alpha, beta, and gamma / Recombinant expression: No
MassTheoretical: 70 kDa
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #6: protein, TMCO1

ProteinName: TMCO1 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #7: protein, NOMO-Nicalin-TMEM147 Complex

ProteinName: NOMO-Nicalin-TMEM147 Complex / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #8: protein, CCDC47

ProteinName: CCDC47 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #9: protein, 60S acidic ribosomal protein P0

ProteinName: 60S acidic ribosomal protein P0 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #10: protein, 60S acidic ribosomal protein P1

ProteinName: 60S acidic ribosomal protein P1 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #11: protein, 60S acidic ribosomal protein P2

ProteinName: 60S acidic ribosomal protein P2 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #12: protein, 60S ribosomal protein L10

ProteinName: 60S ribosomal protein L10 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #13: protein, 60S ribosomal protein L10a

ProteinName: 60S ribosomal protein L10a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #14: protein, 60S ribosomal protein L11

ProteinName: 60S ribosomal protein L11 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #15: protein, 60S ribosomal protein L12

ProteinName: 60S ribosomal protein L12 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #16: protein, 60S ribosomal protein L13

ProteinName: 60S ribosomal protein L13 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #17: protein, 60S ribosomal protein L13a

ProteinName: 60S ribosomal protein L13a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #18: protein, 60S ribosomal protein L14

ProteinName: 60S ribosomal protein L14 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #19: protein, 60S ribosomal protein L15

ProteinName: 60S ribosomal protein L15 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #20: protein, 60S ribosomal protein L17

ProteinName: 60S ribosomal protein L17 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #21: protein, 60S ribosomal protein L18

ProteinName: 60S ribosomal protein L18 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #22: protein, 60S ribosomal protein L18a

ProteinName: 60S ribosomal protein L18a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #23: protein, 60S ribosomal protein L19

ProteinName: 60S ribosomal protein L19 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #24: protein, 60S ribosomal protein L21

ProteinName: 60S ribosomal protein L21 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #25: protein, 60S ribosomal protein L22

ProteinName: 60S ribosomal protein L22 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #26: protein, 60S ribosomal protein L23

ProteinName: 60S ribosomal protein L23 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #27: protein, 60S ribosomal protein L23a

ProteinName: 60S ribosomal protein L23a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #28: protein, 60S ribosomal protein L24

ProteinName: 60S ribosomal protein L24 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #29: protein, 60S ribosomal protein L26

ProteinName: 60S ribosomal protein L26 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #30: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #31: protein, 60S ribosomal protein L27a

ProteinName: 60S ribosomal protein L27a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #32: protein, 60S ribosomal protein L28

ProteinName: 60S ribosomal protein L28 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #33: protein, 60S ribosomal protein L3

ProteinName: 60S ribosomal protein L3 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #34: protein, 60S ribosomal protein L30

ProteinName: 60S ribosomal protein L30 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #35: protein, 60S ribosomal protein L31

ProteinName: 60S ribosomal protein L31 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #36: protein, 60S ribosomal protein L32

ProteinName: 60S ribosomal protein L32 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #37: protein, 60S ribosomal protein L34

ProteinName: 60S ribosomal protein L34 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #38: protein, 60S ribosomal protein L35

ProteinName: 60S ribosomal protein L35 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #39: protein, 60S ribosomal protein L35a

ProteinName: 60S ribosomal protein L35a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #40: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #41: protein, 60S ribosomal protein L36a

ProteinName: 60S ribosomal protein L36a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #42: protein, 60S ribosomal protein L37a

ProteinName: 60S ribosomal protein L37a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #43: protein, 60S ribosomal protein L38

ProteinName: 60S ribosomal protein L38 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #44: protein, 60S ribosomal protein L39

ProteinName: 60S ribosomal protein L39 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #45: protein, 60S ribosomal protein L4

ProteinName: 60S ribosomal protein L4 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #46: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #47: protein, 60S ribosomal protein L6

ProteinName: 60S ribosomal protein L6 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #48: protein, 60S ribosomal protein L7

ProteinName: 60S ribosomal protein L7 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #49: protein, 60S ribosomal protein L7a

ProteinName: 60S ribosomal protein L7a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #50: protein, 60S ribosomal protein L8

ProteinName: 60S ribosomal protein L8 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #51: protein, 60S ribosomal protein L9

ProteinName: 60S ribosomal protein L9 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #52: protein, 40S ribosomal protein S10

ProteinName: 40S ribosomal protein S10 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #53: protein, 40S ribosomal protein S11

ProteinName: 40S ribosomal protein S11 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #54: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #55: protein, 40S ribosomal protein S13

ProteinName: 40S ribosomal protein S13 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #56: protein, 40S ribosomal protein S14

ProteinName: 40S ribosomal protein S14 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #57: protein, 40S ribosomal protein S15

ProteinName: 40S ribosomal protein S15 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #58: protein, 40S ribosomal protein S15a

ProteinName: 40S ribosomal protein S15a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #59: protein, 40S ribosomal protein S16

ProteinName: 40S ribosomal protein S16 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #60: protein, 40S ribosomal protein S17

ProteinName: 40S ribosomal protein S17 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #61: protein, 40S ribosomal protein S18

ProteinName: 40S ribosomal protein S18 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #62: protein, 40S ribosomal protein S19

ProteinName: 40S ribosomal protein S19 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #63: protein, 40S ribosomal protein S2

ProteinName: 40S ribosomal protein S2 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #64: protein, 40S ribosomal protein S21

ProteinName: 40S ribosomal protein S21 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #65: protein, 40S ribosomal protein S23

ProteinName: 40S ribosomal protein S23 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #66: protein, 40S ribosomal protein S24

ProteinName: 40S ribosomal protein S24 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #67: protein, 40S ribosomal protein S25

ProteinName: 40S ribosomal protein S25 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #68: protein, 40S ribosomal protein S26

ProteinName: 40S ribosomal protein S26 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

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Component #69: protein, 40S ribosomal protein S27

ProteinName: 40S ribosomal protein S27 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #70: protein, 40S ribosomal protein S27-like

ProteinName: 40S ribosomal protein S27-like / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #71: protein, 40S ribosomal protein S28

ProteinName: 40S ribosomal protein S28 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #72: protein, 40S ribosomal protein S29

ProteinName: 40S ribosomal protein S29 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #73: protein, 40S ribosomal protein S3

ProteinName: 40S ribosomal protein S3 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #74: protein, 40S ribosomal protein S30

ProteinName: 40S ribosomal protein S30 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #75: protein, 40S ribosomal protein S3a

ProteinName: 40S ribosomal protein S3a / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #76: protein, 40S ribosomal protein S4, X isoform

ProteinName: 40S ribosomal protein S4, X isoformRibosome / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #77: protein, 40S ribosomal protein S5

ProteinName: 40S ribosomal protein S5 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #78: protein, 40S ribosomal protein S6

ProteinName: 40S ribosomal protein S6 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #79: protein, 40S ribosomal protein S7

ProteinName: 40S ribosomal protein S7 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #80: protein, 40S ribosomal protein S8

ProteinName: 40S ribosomal protein S8 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #81: protein, 40S ribosomal protein S9

ProteinName: 40S ribosomal protein S9 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

+
Component #82: protein, 40S ribosomal protein SA

ProteinName: 40S ribosomal protein SA / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (natural)Organelle: Endoplasmic Reticulum / Location in cell: Endoplasmic Reticulum / Cell: HEK293 / Organ Or Tissue: Kidney

-
Experimental details

-
Sample preparation

SpecimenSpecimen State: Particle / Method: cryo EM
Sample solutionSpecimen conc.: 0.4 mg/mL / pH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen Name: ETHANE / Temperature: 298 K / Humidity: 100 %
Details: Two filter papers were added to each arm, 2.5 microliters of sample were added to grids, and 0.5 second of drain time was allowed before vitrification..

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron Source: FIELD EMISSION GUN / Accelerating Voltage: 300 kV / Electron Dose: 50 e/Å2 / Illumination Mode: FLOOD BEAM
LensImaging Mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: OTHER

-
Image acquisition

Image acquisitionNumber of Digital Images: 5562

-
Image processing

ProcessingMethod: single particle reconstruction / Applied Symmetry: C1 (asymmetric) / Number of Projections: 82684
3D reconstructionSoftware: RELION / Resolution: 3.4 Å / Resolution Method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

+
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