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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-31106 | |||||||||
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Title | Snf5 Finger Helix bound to the nucleosome | |||||||||
![]() | The structure of Snf5 Finger Helix bound to the nucleosome | |||||||||
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![]() | Chromatin remodeler complex / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex | |||||||||
Function / homology | ![]() carbon catabolite activation of transcription from RNA polymerase II promoter / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / positive regulation of invasive growth in response to glucose limitation / SWI/SNF complex / nuclear chromosome / transcription coregulator activity / double-strand break repair via homologous recombination / structural constituent of chromatin / nucleosome / nucleosome assembly ...carbon catabolite activation of transcription from RNA polymerase II promoter / positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter / positive regulation of invasive growth in response to glucose limitation / SWI/SNF complex / nuclear chromosome / transcription coregulator activity / double-strand break repair via homologous recombination / structural constituent of chromatin / nucleosome / nucleosome assembly / RNA polymerase II-specific DNA-binding transcription factor binding / chromatin remodeling / protein heterodimerization activity / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Chen ZC / Chen KJ | |||||||||
![]() | ![]() Title: Structure of the SWI/SNF complex bound to the nucleosome and insights into the functional modularity. Authors: Zhenyu He / Kangjing Chen / Youpi Ye / Zhucheng Chen / ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 8.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.1 KB 19.1 KB | Display Display | ![]() |
Images | ![]() | 89.8 KB | ||
Filedesc metadata | ![]() | 6.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 325.6 KB | Display | ![]() |
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Full document | ![]() | 325.2 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 8.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7eg6MC ![]() 7egmC ![]() 7egpC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | The structure of Snf5 Finger Helix bound to the nucleosome | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.0742 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire : Chromatin remodeler complex SWI/SNF, Snf5 Finger Helix bound to t...
+Supramolecule #1: Chromatin remodeler complex SWI/SNF, Snf5 Finger Helix bound to t...
+Supramolecule #2: Snf5 Finger Helix
+Supramolecule #3: Histone
+Supramolecule #4: DNA
+Macromolecule #1: SWI/SNF chromatin-remodeling complex subunit SNF5
+Macromolecule #2: Histone H3.2
+Macromolecule #3: Histone H4
+Macromolecule #4: Histone H2A type 1
+Macromolecule #5: Histone H2B 1.1
+Macromolecule #6: DNA (235-MER)
+Macromolecule #7: DNA (235-MER)
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 525389 |
Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: OTHER |