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- EMDB-31136: The SRM module of SWI/SNF-nucleosome complex -

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Basic information

Entry
Database: EMDB / ID: EMD-31136
TitleThe SRM module of SWI/SNF-nucleosome complex
Map dataThe SRM module of SWI/SNF-nucleosome complex
Sample
  • Complex: Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF
    • Protein or peptide: Transcription regulatory protein SNF2
    • Protein or peptide: SWI/SNF chromatin-remodeling complex subunit SWI1
    • Protein or peptide: SWI/SNF chromatin-remodeling complex subunit SNF5
    • Protein or peptide: SWI/SNF complex subunit SWI3
    • Protein or peptide: Transcription regulatory protein SNF12
    • Protein or peptide: Transcription regulatory protein SNF6
    • Protein or peptide: SWI/SNF global transcription activator complex subunit SWP82
KeywordsChromatin remodeler complex / DNA BINDING PROTEIN
Function / homology
Function and homology information


carbon catabolite activation of transcription / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of mating type switching / positive regulation of invasive growth in response to glucose limitation / aggrephagy / DNA strand invasion / HDACs deacetylate histones / rDNA binding / nucleosome array spacer activity / RSC-type complex ...carbon catabolite activation of transcription / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of mating type switching / positive regulation of invasive growth in response to glucose limitation / aggrephagy / DNA strand invasion / HDACs deacetylate histones / rDNA binding / nucleosome array spacer activity / RSC-type complex / ATP-dependent chromatin remodeler activity / SUMOylation of chromatin organization proteins / SWI/SNF complex / nucleosomal DNA binding / nuclear chromosome / positive regulation of transcription by RNA polymerase I / histone reader activity / histone H4 reader activity / maturation of LSU-rRNA / : / transcription initiation-coupled chromatin remodeling / cellular response to amino acid starvation / nucleotide-excision repair / helicase activity / double-strand break repair via homologous recombination / chromatin DNA binding / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA-templated DNA replication / structural constituent of chromatin / double-strand break repair / histone binding / RNA polymerase II-specific DNA-binding transcription factor binding / hydrolase activity / transcription cis-regulatory region binding / chromatin remodeling / chromatin binding / regulation of transcription by RNA polymerase II / chromatin / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / ATP binding / nucleus / cytosol
Similarity search - Function
Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / : / SMARCC, C-terminal / SWIRM-associated region 1 / SNF5/SMARCB1/INI1 / SNF5 / SMARCB1 / INI1 / Glutamine-Leucine-Glutamine, QLQ / QLQ / QLQ domain profile. ...Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / : / SMARCC, C-terminal / SWIRM-associated region 1 / SNF5/SMARCB1/INI1 / SNF5 / SMARCB1 / INI1 / Glutamine-Leucine-Glutamine, QLQ / QLQ / QLQ domain profile. / QLQ / Snf2-ATP coupling, chromatin remodelling complex / Snf2, ATP coupling domain / Snf2-ATP coupling, chromatin remodelling complex / DNA binding domain with preference for A/T rich regions / AT hook, DNA-binding motif / ARID DNA-binding domain / ARID DNA-binding domain superfamily / ARID/BRIGHT DNA binding domain / ARID domain profile. / BRIGHT, ARID (A/T-rich interaction domain) domain / ARID/BRIGHT DNA binding domain / Helicase/SANT-associated domain / HSA domain profile. / SWIRM domain / SWIRM domain / SWIRM domain profile. / SWIB/MDM2 domain superfamily / SANT domain profile. / SANT domain / Myb-like DNA-binding domain / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Homeobox-like domain superfamily / Helicase conserved C-terminal domain / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
SWI/SNF chromatin-remodeling complex subunit SWI1 / SWI/SNF chromatin-remodeling complex subunit SNF5 / Transcription regulatory protein SNF6 / Transcription regulatory protein SNF2 / SWI/SNF complex subunit SWI3 / SWI/SNF global transcription activator complex subunit SWP82 / Transcription regulatory protein SNF12
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsChen ZC / Chen KJ
CitationJournal: Cell Discov / Year: 2021
Title: Structure of the SWI/SNF complex bound to the nucleosome and insights into the functional modularity.
Authors: Zhenyu He / Kangjing Chen / Youpi Ye / Zhucheng Chen /
History
DepositionMar 24, 2021-
Header (metadata) releaseJan 12, 2022-
Map releaseJan 12, 2022-
UpdateJun 5, 2024-
Current statusJun 5, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7egm
  • Surface level: 0.0125
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_31136.png

Downloads & links

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Map

FileDownload / File: emd_31136.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe SRM module of SWI/SNF-nucleosome complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 400 pix.
= 429.68 Å		1.07 Å/pix.
x 400 pix.
= 429.68 Å		1.07 Å/pix.
x 400 pix.
= 429.68 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0742 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0125 / Movie #1: 0.0125
Minimum - Maximum-0.028471509 - 0.06287943
Average (Standard dev.)0.000069645524 (±0.0010552361)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 429.68002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.07421.07421.0742
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z429.680429.680429.680
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0280.0630.000

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Supplemental data

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Sample components

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Entire : Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF

EntireName: Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF
Components
  • Complex: Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF
    • Protein or peptide: Transcription regulatory protein SNF2
    • Protein or peptide: SWI/SNF chromatin-remodeling complex subunit SWI1
    • Protein or peptide: SWI/SNF chromatin-remodeling complex subunit SNF5
    • Protein or peptide: SWI/SNF complex subunit SWI3
    • Protein or peptide: Transcription regulatory protein SNF12
    • Protein or peptide: Transcription regulatory protein SNF6
    • Protein or peptide: SWI/SNF global transcription activator complex subunit SWP82

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Supramolecule #1: Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF

SupramoleculeName: Substrate Recruitment Module of Chromatin remodeler complex SWI/SNF
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Macromolecule #1: Transcription regulatory protein SNF2

MacromoleculeName: Transcription regulatory protein SNF2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 114.311914 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: LQDQYKEGIK VVDIDDPDMM VDSFTMPNIS HSNIDYQTLL ANSDHAKFTI EPGVLPVGID THTATDIYQT LIALNLDTTV NDCLDKLLN DECTESTREN ALYDYYALQL LPLQKAVRGH VLQFEWHQNS LLTNTHPNFL SKIRNINVQD ALLTNQLYKN H ELLKLERK ...String:
LQDQYKEGIK VVDIDDPDMM VDSFTMPNIS HSNIDYQTLL ANSDHAKFTI EPGVLPVGID THTATDIYQT LIALNLDTTV NDCLDKLLN DECTESTREN ALYDYYALQL LPLQKAVRGH VLQFEWHQNS LLTNTHPNFL SKIRNINVQD ALLTNQLYKN H ELLKLERK KTEAVARLKS MNKSAINQYN RRQDKKNKRL KFGHRLIATH TNLERDEQKR AEKKAKERLQ ALKANDEEAY IK LLDQTKD TRITHLLRQT NAFLDSLTRA VKDQQKYTKE MIDSHIKEAS EEVDDLSMVP KMKDEEYDDD DDNSNVDYYN VAH RIKEDI KKQPSILVGG TLKDYQIKGL QWMVSLFNNH LNGILADEMG LGKTIQTISL LTYLYEMKNI RGPYLVIVPL STLS NWSSE FAKWAPTLRT ISFKGSPNER KAKQAKIRAG EFDVVLTTFE YIIKERALLS KVKWVHMIID EGHRMKNAQS KLSLT LNTH YHADYRLILT GTPLQNNLPE LWALLNFVLP KIFNSVKSFD EWFNTPFANT GGQDKIELSE EETLLVIRRL HKVLRP FLL RRLKKDVEKE LPDKVEKVVK CKMSALQQIM YQQMLKYRRL FIGDQNNKKM VGLRGFNNQI MQLKKICNHP FVFEEVE DQ INPTRETNDD IWRVAGKFEL LDRILPKLKA TGHRVLIFFQ MTQIMDIMED FLRYINIKYL RLDGHTKSDE RSELLRLF N APDSEYLCFI LSTRAGGLGL NLQTADTVII FDTDWNPHQD LQAQDRAHRI GQKNEVRILR LITTNSVEEV ILERAYKKL DIDGKVIQAG KFDNKSTSEE QEALLRSLLD AEEERRKKRE SGVEEEEELK DSEINEILAR NDEEMAVLTR MDEDRSKKEE ELGVKSRLL EKSELPDIYS RDIGAELKRE ESESAAVYNG RGARERKTAT YNDNMSEEQW LRQFEVSDDE KNDKQARKQR T KKEDKSEA IDGGGSGGHH HHHH

UniProtKB: Transcription regulatory protein SNF2

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Macromolecule #2: SWI/SNF chromatin-remodeling complex subunit SWI1

MacromoleculeName: SWI/SNF chromatin-remodeling complex subunit SWI1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 124.885695 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: SNQLISNYAA SNSMDRSSSA SNEFVPNTSD NNNNSNNHNM RNNSNNKTSN NNNVTAVPAA TPANTNNSTS NANTVFSERA AMFAALQQK QQQRFQALQQ QQQQQQNQQQ QNQQPQQQQQ QQQNPKFLQS QRQQQQRSIL QSLNPALQEK ISTELNNKQY E LFMKSLIE ...String:
SNQLISNYAA SNSMDRSSSA SNEFVPNTSD NNNNSNNHNM RNNSNNKTSN NNNVTAVPAA TPANTNNSTS NANTVFSERA AMFAALQQK QQQRFQALQQ QQQQQQNQQQ QNQQPQQQQQ QQQNPKFLQS QRQQQQRSIL QSLNPALQEK ISTELNNKQY E LFMKSLIE NCKKRNMPLQ SIPEIGNRKI NLFYLYMLVQ KFGGADQVTR TQQWSMVAQR LQISDYQQLE SIYFRILLPY ER HMISQEG IKETQAKRIF LQQFLQELLK KVQQQQQAAA LANANNNINS ASSAPTPAAP GASVPATAAP GTEAGIVPVS ANT PKSLNS NININVNNNN IGQQQVKKPR KQRVKKKTKK ELELERKERE DFQKRQQKLL EDQQRQQKLL LETKLRQQYE IELK KLPKV YKRSIVRNYK PLINRLKHYN GYDINYISKI GEKIDSNKPI FLFAPELGAI NLHALSMSLQ SKNLGEINTA LNTLL VTSA DSNLKISLVK YPELLDSLAI LGMNLLSNLS QNVVPYHRNT SDYYYEDAGS NQYYVTQHDK MVDKIFEKVN NNATLT PND SNDEKVTILV DSLTGNQLPT PTPTEMEPDL DTECFISMQS TSPAVKQWDL LPEPIRFLPN QFPLKIHRTP YLTSLKK IK DEIDDPFTKI NTRGAEDPKV LINDQLSTIS MILRNISFSD NNSRIMSRNF YLKRFISDLL WLVLIHPENF TCNRKILN F KKDLVIVLSN ISHLLEIASS IDCLLILILV ISFGQPKLNP MASSSSFGSE SLTFNEFQLQ WGKYQTFGVD ILAKLFSLE KPNLNYFKSI LLNKNTGNNL YDRNSNNNHK DKKLLRRLLN LYNDNNKNNN NRHNLLNDVV SFLFSAIPLQ QVLSQSADPS LLIDQFSPV ISQSLTSILV IVQKILPLSN EVFEISENNS DSNSNNNGNK DSSFNFNKNL PFVWLSSEEN IGSGLLKLSE I ILNINNST SKNTLLQQQN YSKVLLPSIN ISCVQLIKCL VEKSICFENC LNNDPEILKK IASIPNLFPT DLEIFQLFTN PS VDIQIIN QYQLLYNLKN DILTNLEGGS GGWSHPQFEK WSHPQFEKWS HPQFEK

UniProtKB: SWI/SNF chromatin-remodeling complex subunit SWI1

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Macromolecule #3: SWI/SNF chromatin-remodeling complex subunit SNF5

MacromoleculeName: SWI/SNF chromatin-remodeling complex subunit SNF5 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 103.954438 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: MNNQPQGTNS VPNSIGNIFS NIGTPSFNMA QIPQQLYQSL TPQQLQMIQQ RHQQLLRSRL QQQQQQQQQT SPPPQTHQSP PPPPQQSQP IANQSATSTP PPPPAPHNLH PQIGQVPLAP APINLPPQIA QLPLATQQQV LNKLRQQAIA KNNPQVVNAI T VAQQQVQR ...String:
MNNQPQGTNS VPNSIGNIFS NIGTPSFNMA QIPQQLYQSL TPQQLQMIQQ RHQQLLRSRL QQQQQQQQQT SPPPQTHQSP PPPPQQSQP IANQSATSTP PPPPAPHNLH PQIGQVPLAP APINLPPQIA QLPLATQQQV LNKLRQQAIA KNNPQVVNAI T VAQQQVQR QIEQQKGQQT AQTQLEQQRQ LLVQQQQQQQ LRNQIQRQQQ QQFRHHVQIQ QQQQKQQQQQ QQHQQQQQQQ QQ QQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QGQIPQSQQV PQVRSMSGQP PTNVQPTIGQ LPQLPKLNLP KYQTIQYDPP ETK LPYPTY WSDKKADTDT LLYEQIIQRD KINKYSLIRE TNGYDPFSIY GFSNKEYISR LWHTLKYYQD LKNTRMKSIT STSQ KIPSA SIWGNGYSGY GNGITNTTTR VIPQVEVGNR KHYLEDKLKV YKQAMNETSE QLVPIRLEFD QDRDRFFLRD TLLWN KNDK LIKIEDFVDD MLRDYRFEDA TREQHIDTIC QSIQEQIQEF QGNPYIELNQ DRLGGDDLRI RIKLDIVVGQ NQLIDQ FEW DISNSDNCPE EFAESMCQEL ELPGEFVTAI AHSIREQVHM YHKSLALLGY NFDGSAIEDD DIRSRMLPTI TLDDVYR PA AESKIFTPNL LQISAAELER LDKDKDRDTR RKRRQGRSNR RGMLALSGTS ASNTSMNGVH NTVAAGNASS LPPGEILL P DIADIPRTFR TPVPSTLMPG GVDVGPSVES YELRNTTTYK SRPDRPKPVS PPCYIIDHIP GHSLLLSIKL PGKVNTKEE FAAAPNDTSS GTNAMLPSPE SLKTKLNSNI RAGVTIPSIP NPIANHTVTN SPNPTLQPVI PGGAASKSVP TPSLPIAPPV APHDSEATL LTNSNNGSSN NNTQNTGGSG GDYKDDDDK

UniProtKB: SWI/SNF chromatin-remodeling complex subunit SNF5

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Macromolecule #4: SWI/SNF complex subunit SWI3

MacromoleculeName: SWI/SNF complex subunit SWI3 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 94.178352 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: MENTLGEGST VNASVDVDQH GNDNNSDSNA NAAVAGVANT DTAGEESQQQ DESLKDEATV PNTRDAESEA ITVTAKQQPT MQANKLDSQ ETPSTEESRA QNVFGQDNED SDNLFGETES SVSNNEANTP SIPTNPVDNE NNKPAIKEDS TIQDSNGDVK N MEDVKIQK ...String:
MENTLGEGST VNASVDVDQH GNDNNSDSNA NAAVAGVANT DTAGEESQQQ DESLKDEATV PNTRDAESEA ITVTAKQQPT MQANKLDSQ ETPSTEESRA QNVFGQDNED SDNLFGETES SVSNNEANTP SIPTNPVDNE NNKPAIKEDS TIQDSNGDVK N MEDVKIQK EEEPENNTVI EGVKEESQPD ENTKEMDEVE EDDEDDDQPM ISPDNSIFGD TKSESKQLGN TSSVANTPSE IP DAHKAEQ EDIIEKTESV DKKVDSGEER NEQEREIMND HSKSANPKKT TITRVEPETF EIPQAHEIVI PSYSKWFNLE KIH SIEVQS LPEFFTNRIP SKTPEVYMRY RNFMVNSYRL NPNEYFSVTT ARRNVSGDAA ALFRLHKFLT KWGLINYQVD SKLL PKNIE PPLTSQYSTR HDAPRGLFPF ESYKPSVQLP DMAKLKKMMN TSDSESTLYK YLKESKRKYD EITHPPSTTD DENGD KNDN GGKMNNEVST STSMTGDANL LEEGETSRPL KKVKILEQID ENWSKEDLQK LLKGIQEFGA DWYKVAKNVG NKSPEQ CIL RFLQLPIEDK FLYGDGNGKG DNDNGLGPLK YAPHLPFSKS ENPVLSTIAF LVGLVNPKTV QSMTQRAIQS AESIKSQ KE EISDQKPIEH IKEGSEIAIS SLGYRSHIFA TNEERQMNFL TNELIRLQME KLDAKLNHLK KLEKFMELER KTLERQQE N LLIQRLNFNQ NSSKIVNVLS KCLNLISDSN INNSSVAEKE EIRSQIDHFK SMLSKPETLS IGKNPFNKPN IETGENHNG QSISNENDVK PISIEAPQFY RYWSAGGSGG HHHHHH

UniProtKB: SWI/SNF complex subunit SWI3

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Macromolecule #5: Transcription regulatory protein SNF12

MacromoleculeName: Transcription regulatory protein SNF12 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 63.947633 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: MSKVMKPSNG KGSRKSSKAA TPDTKNFFHA KKKDPVNQDK ANNASQITPT VPHSHPSDMV IPDHLAELIP ELYSFQQLVD SEKRLDHFI HLRNLHMKRM VAQWERSKLS QEFLYPHLNF PNVKFLRIFI SNVSENQPWQ MDTNNEADLM ALENATWTMR I EGRLLDNV ...String:
MSKVMKPSNG KGSRKSSKAA TPDTKNFFHA KKKDPVNQDK ANNASQITPT VPHSHPSDMV IPDHLAELIP ELYSFQQLVD SEKRLDHFI HLRNLHMKRM VAQWERSKLS QEFLYPHLNF PNVKFLRIFI SNVSENQPWQ MDTNNEADLM ALENATWTMR I EGRLLDNV QANDPAREKF SSFIESIVVD FKNKENDNVP STKFNAAPEE NATEGPSDKK LNLNLPLQFS LPNGDNSTTT NT DQNNATM GEETAKKDMS STTPKLESVK WQYDPNNPVD FDGLDIKRVG SENVECTISI LRKSSPEEPF MSYSPQLTAI IGL KSGTSH DAIFSIYKYI HLNELLTNDE SAFENLMGNR NNHNSNTSTS KMLDAASSQV SIVKLDTQLI TLLPSSLKES SPDT MKLTD LLSLINSTHL LPLQPIEIDY TVRVDKASTY GELVLDIEVP DVNALKFNNT QRESQIGAAE LNENARELEQ IKPKI ALQD KEITSVLSNL HESNKRYRFF KKISEDPVKA LNECIASTSN ALKVLSGDEG YNEDMVRRAN FYKENEAMLR ENIEVI LSN GRM

UniProtKB: Transcription regulatory protein SNF12

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Macromolecule #6: Transcription regulatory protein SNF6

MacromoleculeName: Transcription regulatory protein SNF6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 37.652582 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: MGVIKKKRSH HGKASRQQYY SGVQVGGVGS MGAINNNIPS LTSFAEENNY QYGYSGSSAG MNGRSLTYAQ QQLNKQRQDF ERVRLRPEQ LSNIIHDESD TISFRSNLLK NFISSNDAFN MLSLTTVPCD RIEKSRLFSE KTIRYLMQKQ HEMKTQAAEL Q EKPLTPLK ...String:
MGVIKKKRSH HGKASRQQYY SGVQVGGVGS MGAINNNIPS LTSFAEENNY QYGYSGSSAG MNGRSLTYAQ QQLNKQRQDF ERVRLRPEQ LSNIIHDESD TISFRSNLLK NFISSNDAFN MLSLTTVPCD RIEKSRLFSE KTIRYLMQKQ HEMKTQAAEL Q EKPLTPLK YTKLIAAAED GSRSTKDMID AVFEQDSHLR YQPDGVVVHR DDPALVGKLR GDLREAPADY WTHAYRDVLA QY HEAKERI RQKEVTAGEA QDEASLQQQQ QQDLQQQQQV VTTVASQSPH ATATEKEPVP AVVDDPLENM FGDYSNEPFN TNF DDEFGD LDAVFF

UniProtKB: Transcription regulatory protein SNF6

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Macromolecule #7: SWI/SNF global transcription activator complex subunit SWP82

MacromoleculeName: SWI/SNF global transcription activator complex subunit SWP82
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 71.510805 KDa
Recombinant expressionOrganism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4 (others)
SequenceString: MLGEDEGNTV LEKGNNPSVK QGEVGAVFIV PKILIREHER VILKQILQIL DQDELVQPPL DKFPYKKLEL PKYIDELKTR DATNTSYKM IQLDAYGEKK VGSNGELFGG RHYLFNTFTF TAHMGVLLVL LQDVIKVLYQ SNATHDEDEF IVQHDQILVM E TSEEQTKF ...String:
MLGEDEGNTV LEKGNNPSVK QGEVGAVFIV PKILIREHER VILKQILQIL DQDELVQPPL DKFPYKKLEL PKYIDELKTR DATNTSYKM IQLDAYGEKK VGSNGELFGG RHYLFNTFTF TAHMGVLLVL LQDVIKVLYQ SNATHDEDEF IVQHDQILVM E TSEEQTKF LAKNGVIPEE SKGSFKYITA RSAFVEFGAS VIAGGQRIVD DYWESLAKKQ NLSSHQRVFK LSTNLISKIS LL RPSFQNN RISNANEISA NTNNTCTIST SKFESQYPIV TEQPSAEIRE AYIENFAKGE HISAIVPGQS ISGTLELSAQ FRV PRYHSK NSFQQALQMK AMDIPIGRHE ELLAQYESQA PDGSASISLP NHIPSVNPSN KPIKRMLSSI LDINVSSSKN KKSE ENEMI KPMNKGQHKN NTSLNINGWK FESLPLKSAE NSGKQQYYRG LPLYEKNTLL ERLKQLTPNE IKELEHLHDA VFVNT GLQN VRKVRTKKWK KYWQYKAGIP IGLKRSQLDE FKNKYLKDVL AQTSVTTNFN EITNTDETIT TKRVPNPNFL GNCNIK DFK PPYIYSHVNK VPQNVAGDKT AVKLDTEVKN TNANPVVATD PVAAKPDNLA NFSNEVAMNN GGSGGHHHHH H

UniProtKB: SWI/SNF global transcription activator complex subunit SWP82

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 210422
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: OTHER

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