EMDB-30432: Cryo-EM structure of 80S ribosome from mouse kidney

Basic information

• Entry: Database: EMDB / ID: EMD-30432
• Title: Cryo-EM structure of 80S ribosome from mouse kidney
• Map data: ribosome from mouse kidney

Sample

• Complex: Mus musculus 80S ribosome from kidney
  • Protein or peptide: x 73 types
  • RNA: x 5 types
• Ligand: x 3 types

• Keywords: 80S mouse ribosome / protein translation / RPL39 / RPL39L / kidney ribosome / RIBOSOME

Function / homology

Function:

5.8S rRNA binding / TNFR1-mediated ceramide production / Protein hydroxylation / positive regulation of amide metabolic process / Formation of the ternary complex, and subsequently, the 43S complex / APC/C:Cdc20 mediated degradation of Cyclin B / SCF-beta-TrCP mediated degradation of Emi1 / APC-Cdc20 mediated degradation of Nek2A / ER Quality Control Compartment (ERQC) / Regulation of PTEN localization / Regulation of pyruvate metabolism / Downregulation of ERBB2:ERBB3 signaling / IRAK2 mediated activation of TAK1 complex / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Gap-filling DNA repair synthesis and ligation in GG-NER / Fanconi Anemia Pathway / Endosomal Sorting Complex Required For Transport (ESCRT) / Negative regulation of FLT3 / Downregulation of TGF-beta receptor signaling / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of expression of SLITs and ROBOs / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Downregulation of ERBB4 signaling / E3 ubiquitin ligases ubiquitinate target proteins / Alpha-protein kinase 1 signaling pathway / Stabilization of p53 / NOTCH3 Activation and Transmission of Signal to the Nucleus / Negative regulators of DDX58/IFIH1 signaling / Pexophagy / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / Translesion synthesis by REV1 / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / Translesion synthesis by POLK / Regulation of NF-kappa B signaling / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Regulation of TP53 Activity through Methylation / Formation of a pool of free 40S subunits / NRIF signals cell death from the nucleus / Translesion synthesis by POLI / Regulation of BACH1 activity / Recognition of DNA damage by PCNA-containing replication complex / p75NTR recruits signalling complexes / HDR through Homologous Recombination (HRR) / Interferon alpha/beta signaling / Negative regulation of MAPK pathway / Spry regulation of FGF signaling / SRP-dependent cotranslational protein targeting to membrane / Regulation of TP53 Degradation / Translesion Synthesis by POLH / Activated NOTCH1 Transmits Signal to the Nucleus / DNA Damage Recognition in GG-NER / Formation of TC-NER Pre-Incision Complex / Major pathway of rRNA processing in the nucleolus and cytosol / Negative regulation of MET activity / TRAF6-mediated induction of TAK1 complex within TLR4 complex / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / Termination of translesion DNA synthesis / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / Senescence-Associated Secretory Phenotype (SASP) / Josephin domain DUBs / Dual Incision in GG-NER / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Ubiquitin-dependent degradation of Cyclin D / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR1-induced NF-kappa-B signaling pathway / Downregulation of ERBB2 signaling / Dual incision in TC-NER / Oncogene Induced Senescence / PINK1-PRKN Mediated Mitophagy / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / SCF(Skp2)-mediated degradation of p27/p21 / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Formation of Incision Complex in GG-NER / Assembly of the pre-replicative complex / CDK-mediated phosphorylation and removal of Cdc6 / Inactivation of CSF3 (G-CSF) signaling / Gap-filling DNA repair synthesis and ligation in TC-NER / TCF dependent signaling in response to WNT / Metalloprotease DUBs / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / EGFR downregulation / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Autodegradation of the E3 ubiquitin ligase COP1 / Regulation of TNFR1 signaling / MAP3K8 (TPL2)-dependent MAPK1/3 activation / G2/M Checkpoints / Degradation of AXIN / Regulation of FZD by ubiquitination / Protein methylation

Domain/homology:

40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S2, eukaryotic / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S17e, conserved site / : / Ribosomal protein S17e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein L44e / Ribosomal protein S30 / Ribosomal protein L44 / Ribosomal protein S30 / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal L40e family / Ribosomal protein S27e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 40S ribosomal protein S4, C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain

Component:

Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL43 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein eS6 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL31 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eL40 fusion protein / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein eS10 / Large ribosomal subunit protein eL22 / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL39 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein eL21 / Small ribosomal subunit protein eS21 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein eS19 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein eL38

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 2.82 Å
• Authors: Huo YG / He X

Funding support

China, 3 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)	31890784	China
National Natural Science Foundation of China (NSFC)	81701511	China
National Natural Science Foundation of China (NSFC)	31830043	China

• Citation: Journal: Nature / Year: 2022; Title: A male germ-cell-specific ribosome controls male fertility.; Authors: Huiling Li / Yangao Huo / Xi He / Liping Yao / Hao Zhang / Yiqiang Cui / Huijuan Xiao / Wenxiu Xie / Dejiu Zhang / Yue Wang / Shu Zhang / Haixia Tu / Yiwei Cheng / Yueshuai Guo / Xintao Cao / Yunfei Zhu / Tao Jiang / Xuejiang Guo / Yan Qin / Jiahao Sha / ; Abstract: Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis. Male germ cell development involves complex translational regulation during sperm formation. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, Ribosome, that is assembled with the male germ-cell-specific protein RPL39L, the paralogue of core ribosome (Ribosome) protein RPL39. Deletion of Ribosome in mice causes defective sperm formation, resulting in substantially reduced fertility. Our comparison of single-particle cryo-electron microscopy structures of ribosomes from mouse kidneys and testes indicates that Ribosome features a ribosomal polypeptide exit tunnel of distinct size and charge states compared with Ribosome. Ribosome predominantly cotranslationally regulates the folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm. Moreover, we found that specialized functions of Ribosome were not replaceable by Ribosome. Taken together, identification of this sperm-specific ribosome should greatly expand our understanding of ribosome function and tissue-specific regulation of protein expression pattern in mammals.

History

Deposition	Aug 8, 2020	-
Header (metadata) release	Feb 2, 2022	-
Map release	Feb 2, 2022	-
Update	May 29, 2024	-
Current status	May 29, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_30432.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: ribosome from mouse kidney
• Voxel size: X=Y=Z: 1.04 Å

Density

Contour Level	By AUTHOR: 0.026 / Movie #1: 0.03
Minimum - Maximum	-0.16205096 - 0.32630816
Average (Standard dev.)	0.00024680863 (±0.012047484)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Y X Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 416.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.04	1.04	1.04
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	416.000	416.000	416.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	400	400	400
MAP C/R/S	2	1	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.162	0.326	0.000

Supplemental data

Sample components

Entire : Mus musculus 80S ribosome from kidney

• Entire: Name: Mus musculus 80S ribosome from kidney

Components

• Complex: Mus musculus 80S ribosome from kidney
  • Protein or peptide: 60S ribosomal protein L8
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: 60S ribosomal protein L11
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L22
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L24
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: 60S ribosomal protein L27a
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: 60S ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: 60S ribosomal protein L36a
  • Protein or peptide: 60S ribosomal protein L37a
  • Protein or peptide: 60S ribosomal protein L28
  • RNA: Mus musculus 28S ribosomal RNA
  • RNA: Mus musculus 5S ribosomal RNA
  • RNA: Mus musculus 5.8S ribosomal RNA
  • RNA: Mus musculus 18S ribosomal RNA
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S30
  • RNA: tRNA
  • Protein or peptide: Ribosomal protein L39
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Mus musculus 80S ribosome from kidney

• Supramolecule: Name: Mus musculus 80S ribosome from kidney / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#78
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 28.088863 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #2: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 32.867941 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA AQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTEWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #3: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 46.212145 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDASKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DTGKKQLEKD FNSMKKYCQV I RIIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFIMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKE EGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #4: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.94201 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGGSS GK AAGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #5: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 47.259926 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV QLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMMNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVKKLEA AATALATKSE KVVPEKGTAD KKPAVGKKGK KVDAKKQKPA GKKV VAKKP AEKKPTTEEK KPAA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #6: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 34.464805 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVRF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVNGGE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #7: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 33.593887 KDa

Sequence

String:

MAGEKAPDTK EKKPAAKKAG SDAAASRPRA AKVAKKVHPK GKKPKKAKPH CSRNPVLVRG IGRYSRSAMY SRKALYKRKY SAAKTKVEK KKKKEKVLAT VTKTVGGDKN GGTRVVKLRK MPRYYPTEDV PRKLLSHGKK PFSQHVRRLR SSITPGTVLI I LTGRHRGK RVVFLKQLDS GLLLVTGPLV INRVPLRRTH QKFVIATSTK VDISDVKIPK HLTDAYFKKK QLRKPRHQEG EI FDTEKEK YEITEQRKAD QKAVDLQILP KIKAVPQLQG YLRSQFSLTN GMYPHKLVF

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #8: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 31.492854 KDa

Sequence

String:

MEAVPEKKKK VATVPGTLKK KVPAGPKTLK KKVPAVPETL KKKRRNFAEL KVKRLRKKFA LKTLRKARRK LIYEKAKHYH KEYRQMYRT EIRMARMARK AGNFYVPAEP KLAFVIRIRG INGVSPKVRK VLQLLRLRQI FNGTFVKLNK ASINMLRIVE P YIAWGYPN LKSVNELIYK RGYGKINKKR IALTDNSLIA RSLGKFGIIC MEDLIHEIYT VGKRFKEANN FLWPFKLSSP RG GMKKKTT HFVEGGDAGN REDQINRLIR RMN

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #9: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.042738 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGHLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #10: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.917484 KDa

Sequence

String:

MKTILSNQTV DIPENVEITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR TGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #11: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.657084 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVI EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #12: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #13: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.36468 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQQ RVDTWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #14: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.620295 KDa

Sequence

String:

MVFRRYVEVG RVAYISFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIDARE RKAKMTDFDR FKVMKAKKMR NRIIKTEVKK LQRAAILKAS PKKAAVAKAA IAAAAAAAAA K AKVPAKKA TGPGKKAAGQ KAPAQKAAGQ KAAPPAKGQK GQKTPAQKAP APKAAGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #15: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #16: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.519354 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL ERLKVLDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKMHY RKKKQILRLR KQAEKNVEKK ICKFTEVLKT NGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #17: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #18: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.698742 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRILEVPKLK VCALRVSSRA RSRILKAGGK ILTFDQLALE SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #19: 60S ribosomal protein L19

• Macromolecule: Name: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #20: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.778486 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAAGKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #21: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.624014 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIIVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #22: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.784962 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #23: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #24: 60S ribosomal protein L24

• Macromolecule: Name: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #25: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #26: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #27: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #28: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.648613 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGVAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #29: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.635156 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAVSARAE AIKALVKPQA IKPKMPKGP KLKRLAFIAH PKLGKRIRSY MAKGQRLCQP KPKVQTKAGA KAPAKAQASA PAQAPKGAQA PKGAQAPVKA P

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #30: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.805092 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #31: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #32: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #33: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #34: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.325022 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT QKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #35: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.595702 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLTKHE EKLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #36: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #37: 60S ribosomal protein L37

• Macromolecule: Name: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #38: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 8.224922 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKDLK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #39: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.758394 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICRKC YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

Macromolecule #40: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #41: 60S ribosomal protein L36a

• Macromolecule: Name: 60S ribosomal protein L36a / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.476973 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #42: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #43: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.770597 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVVVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #48: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 26.715344 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #49: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

Macromolecule #50: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWEAATPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #51: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #52: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #53: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.951818 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGP EGERPARFTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #54: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #55: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #56: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #57: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.552119 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #58: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #59: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.11756 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVRP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #60: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #61: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.154377 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIAAKDHA SIQMNVAEVD RTTGRFNGQF KTYGICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #62: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #63: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #64: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #65: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #66: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #67: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 31.283387 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGLG GRGGFRGGFG SGLRGRGRGR GRGRGRGRGA RGGKAEDKEW IPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #68: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #69: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #70: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #71: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #72: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #73: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #74: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #75: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #76: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.441718 KDa

Sequence

String:

MQLFVRAQEL HTLEVTGQET VAQIKDHVAS LEGIAPEDQV VLLAGSPLED EATLGQCGVE ALTTLEVAGR MLGGKVHGSL ARAGKVRGQ TPKVAKQEKK KKKTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #78: Ribosomal protein L39

• Macromolecule: Name: Ribosomal protein L39 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 6.426759 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #44: Mus musculus 28S ribosomal RNA

• Macromolecule: Name: Mus musculus 28S ribosomal RNA / type: rna / ID: 44 / Number of copies: 1
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 1.530907375 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC GCGCGUCGCG GCGUGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCGGCGCG CCGGGCUCGG GUCUUCCCGG AGUCGGGUUG CUUGGGAAUG CAGCCCAAAG CGGGUGGUAA ACU CCAUCU AAGGCUAAAU ACCGGCACGA GACCGAUAGU CAACAAGUAC CGUAAGGGAA AGUUGAAAAG AACUUUGAAG AGAG AGUUC AAGAGGGCGU GAAACCGUUA AGAGGUAAAC GGGUGGGGUC CGCGCAGUCC GCCCGGAGGA UUCAACCCGG CGGCG GGCG UCCGGCCGUG CCCGGUGGUC CCGGCGGAUC UUUCCCGCUC CCCGUUCCUC CCGACCCCUC CACCCGCGCG UCGUUC CCC UCUUCCUCCC CGCGUCCGGC GCCUCCGGCG GCGGGCGCGG GGGGUGGUGU GGUGGUGGCG CGCGGGCGGG GCCGGGG GU GGGGUCGGCG GGGGACCGUC CCCCGGCCGG CGACCGGCCG CCGCCGGGCG CACUUCCACC GUGGCGGUGC GCCGCGAC C GGCUCCGGGA CGGCCGGGAA GGCCCGGUGG GGAAGGUGGC UCGGGGGGGG CGGCGCGUCU CAGGGCGCGC CGAACCACC UCACCCCGAG UGUUACAGCC CUCCGGCCGC GCUUUCGCCG AAUCCCGGGG CCGAGGAAGC CAGAUACCCG UCGCCGCGCU CUCCCUCUC CCCCCGUCCG CCUCCCGGGC GGGCGUGGGG GUGGGGGCCG GGCCGCCCCU CCCACGGCGC GACCGCUCUC C CACCCCCC UCCGUCGCCU CUCUCGGGGC CCGGUGGGGG GCGGGGCGGA CUGUCCCCAG UGCGCCCCGG GCGUGGUCGC GC CGUCGGG UCCGGGGGGG ACCGUCGGUC ACGCGUCUCC CGACGAAGCC GAGCGCACGG GGUCGGCGGC GAUGUCGGCU ACC CACCCG ACCCGUCUUG AAACACGGAC CAAGGAGUCU AACGCGUGCG CGAGUCAGGG GCUCGUCCGA AAGCCGCCGU GGCG CAAUG AAGGUGAAGG GCCCCGCCCG GGGGCCCGAG GUGGGAUCCC GAGGCCUCUC CAGUCCGCCG AGGGCGCACC ACCGG CCCG UCUCGCCCGC CGCGCCGGGG AGGUGGAGCA CGAGCGUACG CGUUAGGACC CGAAAGAUGG UGAACUAUGC UUGGGC AGG GCGAAGCCAG AGGAAACUCU GGUGGAGGUC CGUAGCGGUC CUGACGUGCA AAUCGGUCGU CCGACCUGGG UAUAGGG GC GAAAGACUAA UCGAACCAUC UAGUAGCUGG UUCCCUCCGA AGUUUCCCUC AGGAUAGCUG GCGCUCUCGC UCCCGACG U ACGCAGUUUU AUCCGGUAAA GCGAAUGAUU AGAGGUCUUG GGGCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUG GGUAAGAAGC CCGGCUCGCU GGCGUGGAGC CGGGCGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGG GAUGAACCGA ACGCCGGGUU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA U AUAGACAG CAGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CC UGAAAAU GGAUGGCGCU GGAGCGUCGG GCCCAUACCC GGCCGUCGCC GGCAGUCGGA ACGGAACGGG ACGGGAGCGG CCG CGGGUG CGCGUCUCUC GGGGUCGGGG GUGCGUGGCG GGGGCCCGUC CCCCGCCUCC CCUCCGCGCG CCGGGUUCGC CCCC GCGGC GUCGGGCCCC GCGGACGCUA CGCCGCGACG AGUAGGAGGG CCGCUGCGGU GAGCCUUGAA GCCUAGGGCG CGGGC CCGG GUGGAGCCGC CGCAGGUGCA GAUCUUGGUG GUAGUAGCAA AUAUUCAAAC GAGAACUUUG AAGGCCGAAG UGGAGA AGG GUUCCAUGUG AACAGCAGUU GAACAUGGGU CAGUCGGUCC UGAGAGAUGG GCGAGUGCCG UUCCGAAGGG ACGGGCG AU GGCCUCCGUU GCCCUCGGCC GAUCGAAAGG GAGUCGGGUU CAGAUCCCCG AAUCCGGAGU GGCGGAGAUG GGCGCCGC G AGGCCAGUGC GGUAACGCGA CCGAUCCCGG AGAAGCCGGC GGGAGCCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGC AGGGCGCCCU GGAAUGGGUU CGCCCCGAGA GAGGGGCCCG UGCCUUGGAA AGCGUCGCGG UUCCGGCGGC GUCCGGUGAG CUCUCGCUG GCCCUUGAAA AUCCGGGGGA GAGGGUGUAA AUCUCGCGCC GGGCCGUACC CAUAUCCGCA GCAGGUCUCC A AGGUGAAC AGCCUCUGGC AUGUUGGAAC AAUGUAGGUA AGGGAAGUCG GCAAGCCGGA UCCGUAACUU CGGGAUAAGG AU UGGCUCU AAGGGCUGGG UCGGUCGGGC UGGGGCGCGA AGCGGGGCUG GGCGCGCGCC GCGGCUGGAC GAGGCGCCGC CGC CCUCUC CCACGUCCGG GGAGACCCCC CGUCCUUUCC GCCCGGGCCC GCCCUCCCCU CUUCCCCGCG GGGCCCCGUC GUCC CCCGC GUCGUCGCCA CCUCUCUUCC CCCCUCCUUC UUCCCGUCGG GGGGCGGGUC GGGGGUCGGC GCGCGGCGCG GGCUC CGGG GCGGCGGGUC CAACCCCGCG GGGGUUCCGG AGCGGGAGGA ACCAGCGGUC CCCGGUGGGG CGGGGGGCCC GGACAC UCG GGGGGCCGGC GGCGGCGGCG ACUCUGGACG CGAGCCGGGC CCUUCCCGUG GAUCGCCUCA GCUGCGGCGG GCGUCGC GG CCGCUCCCGG GGAGCCCGGC GGGUGCCGGC GCGGGUCCCC UCCCCGCGGG GCCUCGCUCC ACCCCCCCAU CGCCUCUC C CGAGGUGCGU GGCGGGGGCG GGCGGGCGUG UCCCGCGCGU GUGGGGGGAA CCUCCGCGUC GGUGUUCCCC CGCCGGGUC CGCCCCCCGG GCCGCGGUUU UCCGCGCGGC GCCCCCGCCU CGGCCGGCGC CUAGCAGCCG ACUUAGAACU GGUGCGGACC AGGGGAAUC CGACUGUUUA AUUAAAACAA AGCAUCGCGA AGGCCCGCGG CGGGUGUUGA CGCGAUGUGA UUUCUGCCCA G UGCUCUGA AUGUCAAAGU GAAGAAAUUC AAUGAAGCGC GGGUAAACGG CGGGAGUAAC UAUGACUCUC UUAAGGUAGC CA AAUGCCU CGUCAUCUAA UUAGUGACGC GCAUGAAUGG AUGAACGAGA UUCCCACUGU CCCUACCUAC UAUCCAGCGA AAC CACAGC CAAGGGAACG GGCUUGGCGG AAUCAGCGGG GAAAGAAGAC CCUGUUGAGC UUGACUCUAG UCUGGCACGG UGAA GAGAC AUGAGAGGUG UAGAAUAAGU GGGAGGCCCC CGGCGCCCGG CCCCGUCCUC GCGUCGGGGU CGGGGCACGC CGGCC UCGC GGGCCGCCGG UGAAAUACCA CUACUCUCAU CGUUUUUUCA CUGACCCGGU GAGGCGGGGG GGCGAGCCCC GAGGGG CUC UCGCUUCUGG CGCCAAGCGU CCGUCCCGCG CGUGCGGGCG GGCGCGACCC GCUCCGGGGA CAGUGCCAGG UGGGGAG UU UGACUGGGGC GGUACACCUG UCAAACGGUA ACGCAGGUGU CCUAAGGCGA GCUCAGGGAG GACAGAAACC UCCCGUGG A GCAGAAGGGC AAAAGCUCGC UUGAUCUUGA UUUUCAGUAC GAAUACAGAC CGUGAAAGCG GGGCCUCACG AUCCUUCUG ACCUUUUGGG UUUUAAGCAG GAGGUGUCAG AAAAGUUACC ACAGGGAUAA CUGGCUUGUG GCGGCCAAGC GUUCAUAGCG ACGUCGCUU UUUGAUCCUU CGAUGUCGGC UCUUCCUAUC AUUGUGAAGC AGAAUUCACC AAGCGUUGGA UUGUUCACCC A CUAAUAGG GAACGUGAGC UGGGUUUAGA CCGUCGUGAG ACAGGUUAGU UUUACCCUAC UGAUGAUGUG UUGUUGCCAU GG UAAUCCU GCUCAGUACG AGAGGAACCG CAGGUUCAGA CAUUUGGUGU AUGUGCUUGG CUGAGGAGCC AAUGGGGCGA AGC UACCAU CUGUGGGAUU AUGACUGAAC GCCUCUAAGU CAGAAUCCCG CCCAGGCGGA ACGAUACGGC AGCGCCGAAG GAGC CUCGG UUGGCCCCGG AUAGCCGGGU CCCCGUCCGU CCCCGCUCGG CGGGGUCCCC GCGUCGUCCC CGCGGCGGCG CGGGG UCUC CCCCCGCCGG GCGUCGGGAC CGGGGUCCGG UGCGGAGAGC CGUUCGUCUU GGGAAACGGG GUGCGGCCGG AAAGGC GGC CGCCCUCUCG CCCGUCACGU UGAACGCACG UUCGUGUGGA ACCUGGCGCU AAACCAUUCG UAGACGACCU GCUUCUG GG UCGGGGUUUC GUACGUAGCA GAGCAGCUCC CUCGCUGCGA UCUAUUGAAA GUCAGCCCUC GACACAAGGG UUUGUCUC U GCGGGCUUUC

Macromolecule #45: Mus musculus 5S ribosomal RNA

• Macromolecule: Name: Mus musculus 5S ribosomal RNA / type: rna / ID: 45 / Number of copies: 1
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 38.691914 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: X71804.1

Macromolecule #46: Mus musculus 5.8S ribosomal RNA

• Macromolecule: Name: Mus musculus 5.8S ribosomal RNA / type: rna / ID: 46 / Number of copies: 1
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.79502 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUUG

GENBANK: GENBANK: AH002077.2

Macromolecule #47: Mus musculus 18S ribosomal RNA

• Macromolecule: Name: Mus musculus 18S ribosomal RNA / type: rna / ID: 47 / Number of copies: 1
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 603.101938 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCCU UCCCGGGGGG GGAUGCGUGC AUUUAUCAGA UCAAAACCAA CC CGGUGAG CUCCCUCCCG GCUCCGGCCG GGGGUCGGGC GCCGGCGGCU UGGUGACUCU AGAUAACCUC GGGCCGAUCG CAC GCCCCC CGUGGCGGCG ACGACCCAUU CGAACGUCUG CCCUAUCAAC UUUCGAUGGU AGUCGCCGUG CCUACCAUGG UGAC CACGG GUGACGGGGA AUCAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAAACGGCU ACCACAUCCA AGGAAGGCAG CAGGC GCGC AAAUUACCCA CUCCCGACCC GGGGAGGUAG UGACGAAAAA UAACAAUACA GGACUCUUUC GAGGCCCUGU AAUUGG AAU GAGUCCACUU UAAAUCCUUU AACGAGGAUC CAUUGGAGGG CAAGUCUGGU GCCAGCAGCC GCGGUAAUUC CAGCUCC AA UAGCGUAUAU UAAAGUUGCU GCAGUUAAAA AGCUCGUAGU UGGAUCUUGG GAGCGGGCGG GCGGUCCGCC GCGAGGCG A GUCACCGCCC GUCCCCGCCC CUUGCCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCGU UUACUUUGAA AAAAUUAGAG UGUUCAAAGC AGGCCCGAGC CGCCUGGAUA CCGCAGCUAG GAAUAAUGGA AUAGGACCGC GGUUCUAUU UUGUUGGUUU UCGGAACUGA GGCCAUGAUU AAGAGGGACG GCCGGGGGCA UUCGUAUUGC GCCGCUAGAG G UGAAAUUC UUGGACCGGC GCAAGACGGA CCAGAGCGAA AGCAUUUGCC AAGAAUGUUU UCAUUAAUCA AGAACGAAAG UC GGAGGUU CGAAGACGAU CAGAUACCGU CGUAGUUCCG ACCAUAAACG AUGCCGACUG GCGAUGCGGC GGCGUUAUUC CCA UGACCC GCCGGGCAGC UUCCGGGAAA CCAAAGUCUU UGGGUUCCGG GGGGAGUAUG GUUGCAAAGC UGAAACUUAA AGGA AUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGAAACCUC ACCCGGCCCG GACAC GGAC AGGAUUGACA GAUUGAUAGC UCUUUCUCGA UUCCGUGGGU GGUGGUGCAU GGCCGUUCUU AGUUGGUGGA GCGAUU UGU CUGGUUAAUU CCGAUAACGA ACGAGACUCU GGCAUGCUAA CUAGUUACGC GACCCCCGAG CGGUCGGCGU CCCCCAA CU UCUUAGAGGG ACAAGUGGCG UUCAGCCACC CGAGAUUGAG CAAUAACAGG UCUGUGAUGC CCUUAGAUGU CCGGGGCU G CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU GCGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUUCGU GAUGGGGAUC GGGGAUUGCA AUUAUUCCCC AUGAACGAGG AAUUCCCAGU AAGUGCGGGU CAUAAGCUUG CGUUGAUUAA GUCCCUGCC CUUUGUACAC ACCGCCCGUC GCUACUACCG AUUGGAUGGU UUAGUGAGGC CCUCGGAUCG GCCCCGCCGG G GUCGGCCC ACGGCCCUGG CGGAGCGCUG AGAAGACGGU CGAACUUGAC UAUCUAGAGG AAGUAAAAGU CGUAACAAGG UU UCCGUAG GUGAACCUGC GGAAGGAUCA UUA

Macromolecule #77: tRNA

• Macromolecule: Name: tRNA / type: rna / ID: 77 / Number of copies: 1
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.23151 KDa

Sequence

String:

AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

Macromolecule #79: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 79 / Number of copies: 271 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #80: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 80 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #81: water

• Macromolecule: Name: water / type: ligand / ID: 81 / Number of copies: 19 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

Buffer

pH: 7.5
Component:

Concentration	Formula	Name
20.0 mM	Tris-HCl	Tris base
100.0 mM	KCl	Potassium chloride
2.0 mM	MgCl2	Magnesium chloride hexahydrate
2.0 mM	DTT	Dithiothreitol

• Grid: Model: Quantifoil / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6eko

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.82 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 280287
• Initial angle assignment: Type: ANGULAR RECONSTITUTION
• Final angle assignment: Type: ANGULAR RECONSTITUTION

Atomic model buiding 1

Initial model

PDB ID:

6ek0
PDB Unreleased entry

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-7cpu:
Cryo-EM structure of 80S ribosome from mouse kidney