The NLRP3 inflammasome / regulation of presynaptic dense core granule exocytosis / Platelet homeostasis / positive regulation of lymphocyte apoptotic process / positive regulation of bleb assembly / NAD transport / phagolysosome assembly / Elevation of cytosolic Ca2+ levels / phospholipid transfer to membrane / positive regulation of cytoskeleton organization ...The NLRP3 inflammasome / regulation of presynaptic dense core granule exocytosis / Platelet homeostasis / positive regulation of lymphocyte apoptotic process / positive regulation of bleb assembly / NAD transport / phagolysosome assembly / Elevation of cytosolic Ca2+ levels / phospholipid transfer to membrane / positive regulation of cytoskeleton organization / organic cation transport / purinergic nucleotide receptor activity / extracellularly ATP-gated monoatomic cation channel activity / lymphocyte apoptotic process / purinergic nucleotide receptor signaling pathway / gamma-aminobutyric acid secretion / positive regulation of monoatomic ion transmembrane transport / pore complex assembly / ATP export / positive regulation of interleukin-1 alpha production / negative regulation of cell volume / cell death / plasma membrane organization / positive regulation of gamma-aminobutyric acid secretion / collagen metabolic process / bleb / : / plasma membrane phospholipid scrambling / response to fluid shear stress / positive regulation of prostaglandin secretion / T cell apoptotic process / bleb assembly / mitochondrial depolarization / ceramide biosynthetic process / vesicle budding from membrane / positive regulation of T cell apoptotic process / prostaglandin secretion / programmed cell death / positive regulation of ossification / cellular response to dsRNA / glutamate secretion / cell volume homeostasis / positive regulation of glutamate secretion / negative regulation of bone resorption / skeletal system morphogenesis / positive regulation of macrophage cytokine production / phospholipid translocation / channel activity / nuclear inner membrane / negative regulation of MAPK cascade / positive regulation of mitochondrial depolarization / positive regulation of calcium ion transport into cytosol / response to ATP / response to zinc ion / positive regulation of catalytic activity / T cell homeostasis / monoatomic cation transport / synaptic vesicle exocytosis / membrane depolarization / membrane protein ectodomain proteolysis / cellular response to organic cyclic compound / protein secretion / neuronal action potential / positive regulation of bone mineralization / T cell proliferation / response to electrical stimulus / response to mechanical stimulus / regulation of sodium ion transport / extrinsic apoptotic signaling pathway / release of sequestered calcium ion into cytosol / homeostasis of number of cells within a tissue / sensory perception of pain / positive regulation of glycolytic process / reactive oxygen species metabolic process / protein serine/threonine kinase activator activity / mitochondrion organization / : / positive regulation of interleukin-1 beta production / positive regulation of cytokine production / establishment of localization in cell / positive regulation of protein secretion / lipopolysaccharide binding / apoptotic signaling pathway / response to bacterium / positive regulation of MAP kinase activity / protein catabolic process / neuromuscular junction / cell morphogenesis / response to organic cyclic compound / T cell mediated cytotoxicity / terminal bouton / protein processing / response to calcium ion / positive regulation of T cell mediated cytotoxicity / calcium ion transport / positive regulation of interleukin-6 production / MAPK cascade / cell-cell junction / presynapse / signaling receptor activity Similarity search - Function
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)
K99HL138129
United States
Citation
Journal: Cell / Year: 2019 Title: Full-Length P2X Structures Reveal How Palmitoylation Prevents Channel Desensitization. Authors: Alanna E McCarthy / Craig Yoshioka / Steven E Mansoor / Abstract: P2X receptors are trimeric, non-selective cation channels activated by extracellular ATP. The P2X receptor subtype is a pharmacological target because of involvement in apoptotic, inflammatory, and ...P2X receptors are trimeric, non-selective cation channels activated by extracellular ATP. The P2X receptor subtype is a pharmacological target because of involvement in apoptotic, inflammatory, and tumor progression pathways. It is the most structurally and functionally distinct P2X subtype, containing a unique cytoplasmic domain critical for the receptor to initiate apoptosis and not undergo desensitization. However, lack of structural information about the cytoplasmic domain has hindered understanding of the molecular mechanisms underlying these processes. We report cryoelectron microscopy structures of full-length rat P2X receptor in apo and ATP-bound states. These structures reveal how one cytoplasmic element, the C-cys anchor, prevents desensitization by anchoring the pore-lining helix to the membrane with palmitoyl groups. They show a second cytoplasmic element with a unique fold, the cytoplasmic ballast, which unexpectedly contains a zinc ion complex and a guanosine nucleotide binding site. Our structures provide first insights into the architecture and function of a P2X receptor cytoplasmic domain.
History
Deposition
Sep 9, 2019
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Header (metadata) release
Oct 2, 2019
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Map release
Oct 23, 2019
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Update
Jul 29, 2020
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Current status
Jul 29, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Map for the ATP-bound (open) state of rat P2X7 receptor from a focused refinement using a mask that includes the transmembrane domain and the cytoplasmic domain but excludes the extracellular domain.
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