- EMDB-11269: E2 core of the fungal Pyruvate dehydrogenase complex with absent ... -
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Basic information
Entry
Database: EMDB / ID: EMD-11269
Title
E2 core of the fungal Pyruvate dehydrogenase complex with absent periphery, structured core and empty interior.
Map data
Fungal PDC (N. crassa). Recombinant preparation-E2. Enforced symmetry: I2. Periphery (E2 only) is flexible/oversym. Core is structured. Interior is empty.
Sample
Complex: E2 core of the fungal Pyruvate dehydrogenase complex with absent periphery, structured core and empty interior.
Biological species
Neurospora crassa (fungus)
Method
single particle reconstruction / cryo EM / Resolution: 4.4 Å
Journal: Nat Commun / Year: 2020 Title: Arrangement and symmetry of the fungal E3BP-containing core of the pyruvate dehydrogenase complex. Authors: B O Forsberg / S Aibara / R J Howard / N Mortezaei / E Lindahl / Abstract: The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) ...The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) of mammalian PDC, PX selectively recruits E3 to the fungal PDC, but its divergent sequence suggests a distinct structural mechanism. Here, we report reconstructions of PDC from the filamentous fungus Neurospora crassa by cryo-electron microscopy, where we find protein X (PX) interior to the PDC core as opposed to substituting E2 core subunits as in mammals. Steric occlusion limits PX binding, resulting in predominantly tetrahedral symmetry, explaining previous observations in Saccharomyces cerevisiae. The PX-binding site is conserved in (and specific to) fungi, and complements possible C-terminal binding motifs in PX that are absent in mammalian E3BP. Consideration of multiple symmetries thus reveals a differential structural basis for E3BP-like function in fungal PDC.
History
Deposition
Jun 30, 2020
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Header (metadata) release
Sep 23, 2020
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Map release
Sep 23, 2020
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Update
Dec 16, 2020
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Current status
Dec 16, 2020
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_11269.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Fungal PDC (N. crassa). Recombinant preparation-E2. Enforced symmetry: I2. Periphery (E2 only) is flexible/oversym. Core is structured. Interior is empty.
Voxel size
X=Y=Z: 1.23 Å
Density
Contour Level
By AUTHOR: 0.044 / Movie #1: 0.044
Minimum - Maximum
-0.07883227 - 0.13346662
Average (Standard dev.)
0.0004900665 (±0.0057209013)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
384
384
384
Spacing
384
384
384
Cell
A=B=C: 472.32 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.23
1.23
1.23
M x/y/z
384
384
384
origin x/y/z
0.000
0.000
0.000
length x/y/z
472.320
472.320
472.320
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
108
134
0
NX/NY/NZ
135
84
349
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
384
384
384
D min/max/mean
-0.079
0.133
0.000
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Supplemental data
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Half map: Half-map 1. Fungal PDC (N. crassa). Recombinant preparation-E2....
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