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- EMDB-11267: E2 core of the fungal Pyruvate dehydrogenase complex with flexibl... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-11267 | ||||||||||||||||||
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Title | E2 core of the fungal Pyruvate dehydrogenase complex with flexible/oversym. periphery, structured core and S4X-structured interior. | ||||||||||||||||||
![]() | Fungal PDC (N. crassa). Endogenous preparation-E1 E2 E3 PX. Enforced symmetry: T. Periphery is flexible/oversym. Core is structured. Interior is structured in arrangement X / S4X. | ||||||||||||||||||
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Biological species | ![]() | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.4 Å | ||||||||||||||||||
![]() | Forsberg BO / Lindahl E / Aibara S / Howard RJ / Mortezaei N | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Arrangement and symmetry of the fungal E3BP-containing core of the pyruvate dehydrogenase complex. Authors: B O Forsberg / S Aibara / R J Howard / N Mortezaei / E Lindahl / ![]() ![]() Abstract: The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) ...The pyruvate dehydrogenase complex (PDC) is a multienzyme complex central to aerobic respiration, connecting glycolysis to mitochondrial oxidation of pyruvate. Similar to the E3-binding protein (E3BP) of mammalian PDC, PX selectively recruits E3 to the fungal PDC, but its divergent sequence suggests a distinct structural mechanism. Here, we report reconstructions of PDC from the filamentous fungus Neurospora crassa by cryo-electron microscopy, where we find protein X (PX) interior to the PDC core as opposed to substituting E2 core subunits as in mammals. Steric occlusion limits PX binding, resulting in predominantly tetrahedral symmetry, explaining previous observations in Saccharomyces cerevisiae. The PX-binding site is conserved in (and specific to) fungi, and complements possible C-terminal binding motifs in PX that are absent in mammalian E3BP. Consideration of multiple symmetries thus reveals a differential structural basis for E3BP-like function in fungal PDC. | ||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 73.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.4 KB 14.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13 KB | Display | ![]() |
Images | ![]() | 192.1 KB | ||
Others | ![]() ![]() | 97.7 MB 97.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 397.6 KB | Display | ![]() |
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Full document | ![]() | 396.7 KB | Display | |
Data in XML | ![]() | 16.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6zlmC ![]() 6zloC C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data size: 305.5 Data #1: Aligned and dose-weighted micrographs of native N. crassa Pyruvate dehydrogenase [micrographs - single frame]) |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Fungal PDC (N. crassa). Endogenous preparation-E1 E2 E3 PX. Enforced symmetry: T. Periphery is flexible/oversym. Core is structured. Interior is structured in arrangement X / S4X. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Half map: Half-map 2. Fungal PDC (N. crassa). Endogenous preparation-E1...
File | emd_11267_half_map_1.map | ||||||||||||
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Annotation | Half-map 2. Fungal PDC (N. crassa). Endogenous preparation-E1 E2 E3 PX. Enforced symmetry: T. Periphery is flexible/oversym. Core is structured. Interior is structured in arrangement X / S4X. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map 1. Fungal PDC (N. crassa). Endogenous preparation-E1...
File | emd_11267_half_map_2.map | ||||||||||||
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Annotation | Half-map 1. Fungal PDC (N. crassa). Endogenous preparation-E1 E2 E3 PX. Enforced symmetry: T. Periphery is flexible/oversym. Core is structured. Interior is structured in arrangement X / S4X. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : endogenous pyruvate dehydrogenase complex form Neurospora crassa
Entire | Name: endogenous pyruvate dehydrogenase complex form Neurospora crassa |
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Components |
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-Supramolecule #1: endogenous pyruvate dehydrogenase complex form Neurospora crassa
Supramolecule | Name: endogenous pyruvate dehydrogenase complex form Neurospora crassa type: complex / ID: 1 / Parent: 0 Details: Catalytic (C-terminal) domain of Dihydrolipoyllysine-residue acetyltransferase (E2-component of pyruvate dehydrogenase complex) |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 7 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: COUNTING / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |