- EMDB-11072: Reconstruction of bovine Ryanodine receptor 2 dimer of tetramers ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-11072
タイトル
Reconstruction of bovine Ryanodine receptor 2 dimer of tetramers in complex with FKBP12.6 and nanobodies
マップデータ
試料
複合体: Dimer of bovine Ryanodine receptor 2
機能・相同性
機能・相同性情報
Stimuli-sensing channels / suramin binding / establishment of protein localization to endoplasmic reticulum / type B pancreatic cell apoptotic process / Purkinje myocyte to ventricular cardiac muscle cell signaling / Ion homeostasis / regulation of SA node cell action potential / regulation of atrial cardiac muscle cell action potential / left ventricular cardiac muscle tissue morphogenesis / regulation of AV node cell action potential ...Stimuli-sensing channels / suramin binding / establishment of protein localization to endoplasmic reticulum / type B pancreatic cell apoptotic process / Purkinje myocyte to ventricular cardiac muscle cell signaling / Ion homeostasis / regulation of SA node cell action potential / regulation of atrial cardiac muscle cell action potential / left ventricular cardiac muscle tissue morphogenesis / regulation of AV node cell action potential / positive regulation of ATPase-coupled calcium transmembrane transporter activity / calcium-induced calcium release activity / ventricular cardiac muscle cell action potential / regulation of ventricular cardiac muscle cell action potential / positive regulation of sequestering of calcium ion / cyclic nucleotide binding / embryonic heart tube morphogenesis / cardiac muscle hypertrophy / negative regulation of insulin secretion involved in cellular response to glucose stimulus / negative regulation of release of sequestered calcium ion into cytosol / ryanodine-sensitive calcium-release channel activity / neuronal action potential propagation / release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / response to muscle activity / insulin secretion / calcium ion transport into cytosol / response to redox state / negative regulation of heart rate / negative regulation of phosphoprotein phosphatase activity / positive regulation of heart rate / FK506 binding / positive regulation of axon regeneration / cellular response to caffeine / protein kinase A regulatory subunit binding / protein kinase A catalytic subunit binding / positive regulation of the force of heart contraction / : / detection of calcium ion / smooth muscle contraction / smooth endoplasmic reticulum / negative regulation of ryanodine-sensitive calcium-release channel activity / response to vitamin E / calcium channel inhibitor activity / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / protein peptidyl-prolyl isomerization / response to glucose / T cell proliferation / chaperone-mediated protein folding / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / release of sequestered calcium ion into cytosol / regulation of ryanodine-sensitive calcium-release channel activity / sarcoplasmic reticulum membrane / calcium channel complex / cellular response to epinephrine stimulus / regulation of cytosolic calcium ion concentration / response to muscle stretch / regulation of heart rate / : / peptidylprolyl isomerase / sarcoplasmic reticulum / peptidyl-prolyl cis-trans isomerase activity / establishment of localization in cell / response to hydrogen peroxide / Z disc / intracellular calcium ion homeostasis / : / positive regulation of cytosolic calcium ion concentration / transmembrane transporter binding / calmodulin binding / response to hypoxia / intracellular membrane-bounded organelle / signaling receptor binding / calcium ion binding / identical protein binding / membrane / cytoplasm 類似検索 - 分子機能
凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 293.15 K / 装置: GATAN CRYOPLUNGE 3 詳細: .Grid was blotted manually from the back in CP3 cryoplunge (Gatan) for 2s.
詳細
The RyR2 tetrameric channels formed channel-dimers with the nanobody at the dimer-interface bound to the repeat12 domain of the individual channels
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
特殊光学系
球面収差補正装置: Microscope was modified with a Cs corrector
撮影
フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 撮影したグリッド数: 1 / 実像数: 4088 / 平均電子線量: 47.0 e/Å2