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- EMDB-0090: Structure of a yeast closed complex (core CC1) -

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Basic information

Entry
Database: EMDB / ID: EMD-0090
TitleStructure of a yeast closed complex (core CC1)
Map data
SampleYeast pre-initiation complexPreinitiation complex
  • RNA polymerase
  • TFs
  • Nucleic acidsNucleic acid
  • (DNA-directed RNA polymerase II subunit ...Polymerase) x 7
  • (DNA-directed RNA polymerases I, II, and III subunit ...RNA polymerase) x 5
  • Transcription initiation factor IIB
  • (nucleic-acidNucleic acid) x 2
  • TATA-box-binding protein
  • (Transcription initiation factor IIF subunit ...) x 2
  • (Transcription initiation factor IIA ...) x 2
  • (ligand) x 2
Function / homology
Function and homology information


promoter clearance from RNA polymerase II promoter / go:0001186: / TFIIA-class transcription factor complex binding / dephosphorylation of RNA polymerase II C-terminal domain / transcriptional open complex formation at RNA polymerase II promoter / transcription factor TFIIF complex / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase II complex recruiting activity / transcription factor TFIIIB complex / transcription factor TFIIA complex ...promoter clearance from RNA polymerase II promoter / go:0001186: / TFIIA-class transcription factor complex binding / dephosphorylation of RNA polymerase II C-terminal domain / transcriptional open complex formation at RNA polymerase II promoter / transcription factor TFIIF complex / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase II complex recruiting activity / transcription factor TFIIIB complex / transcription factor TFIIA complex / RNA polymerase III general transcription initiation factor activity / RNA polymerase I general transcription initiation factor binding / RNA polymerase III preinitiation complex assembly / regulation of protein serine/threonine phosphatase activity / RNA polymerase III regulatory region DNA binding / nuclear-transcribed mRNA catabolic process, exonucleolytic / nucleolar large rRNA transcription by RNA polymerase I / transposon integration / RNA polymerase II general transcription initiation factor activity / recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex / mRNA export from nucleus in response to heat stress / maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter / transcription factor TFIID complex / DNA binding, bending / RNA polymerase II activity / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / positive regulation of transcription regulatory region DNA binding / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA polymerase II complex binding / termination of RNA polymerase III transcription / positive regulation of translational initiation / protein phosphatase activator activity / RNA polymerase I activity / tRNA transcription by RNA polymerase III / RNA polymerase I complex / RNA polymerase III complex / mRNA cleavage / RNA polymerase II, core complex / RNA polymerase II preinitiation complex assembly / transcription by RNA polymerase I / transcription by RNA polymerase III / transcription, RNA-templated / transcriptional preinitiation complex / termination of RNA polymerase II transcription / translation initiation factor binding / transcription coregulator activity / RNA polymerase II core promoter sequence-specific DNA binding / transcription elongation from RNA polymerase II promoter / ribonucleoside binding / single-stranded RNA binding / TBP-class protein binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / DNA-templated transcription, initiation / RNA polymerase II activating transcription factor binding / positive regulation of transcription elongation from RNA polymerase II promoter / P-body / ribosome biogenesis / transcription-coupled nucleotide-excision repair / cytoplasmic stress granule / single-stranded DNA binding / translesion synthesis / disordered domain specific binding / transcription by RNA polymerase II / transcription factor complex / positive regulation of DNA-binding transcription factor activity / transcription initiation from RNA polymerase II promoter / nucleic acid binding / transcription coactivator activity / protein dimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / mRNA binding / transcription factor binding / cellular response to DNA damage stimulus / nucleolus / DNA-binding transcription factor activity / chromatin binding / regulation of transcription, DNA-templated / nucleotide binding / positive regulation of transcription by RNA polymerase II / protein-containing complex / mitochondrion / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
Transcription initiation factor IIA, gamma subunit / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase Rpb4/RPC9, core / Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit / RNA polymerase, subunit omega/K/RPB6 / RNA polymerase Rpb7-like , N-terminal / RNA polymerase subunit RPB4/RPC9 / RNA polymerase, Rpb5, N-terminal / RNA polymerase, Rpb8 ...Transcription initiation factor IIA, gamma subunit / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase Rpb4/RPC9, core / Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit / RNA polymerase, subunit omega/K/RPB6 / RNA polymerase Rpb7-like , N-terminal / RNA polymerase subunit RPB4/RPC9 / RNA polymerase, Rpb5, N-terminal / RNA polymerase, Rpb8 / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIF, beta subunit / S1 domain / DNA-directed RNA polymerase, M/15kDa subunit / Zinc finger, TFIIS-type / RNA polymerase Rpb1, domain 3 / TATA-box binding protein / Transcription factor TFIIB / RNA polymerase, subunit H/Rpb5 C-terminal / RNA polymerase, alpha subunit / RNA polymerase II, heptapeptide repeat, eukaryotic / DNA-directed RNA polymerase, subunit N/Rpb10 / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerase, subunit H/Rpb5, conserved site / Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerase, N-terminal / RNA polymerase Rpb1, domain 7 / RNA-binding domain, S1 / Transcription factor IIA, helical / DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus / Cyclin-like / Transcription factor TFIIB, cyclin-like domain / Zinc finger, TFIIB-type / Nucleic acid-binding, OB-fold / TBP domain superfamily / RNA polymerase subunit, RPB6/omega / DNA-directed RNA polymerase subunit/transcription factor S / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / DNA-directed RNA polymerase, insert domain / Transcription Factor IIF, Rap30/Rap74, interaction / HRDC-like superfamily / Transcription factor IIA, beta-barrel / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb1, domain 6 / Transcription initiation factor IIF, alpha subunit / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 3 / RNA polymerase, beta subunit, protrusion / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 7 / RNA polymerase, beta subunit, conserved site / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 1 / RNA polymerases, subunit N, zinc binding site / RNA polymerase Rpb2, OB-fold / Transcription factor TFIIB, conserved site / RPB6/omega subunit-like superfamily / Winged helix-like DNA-binding domain superfamily / Pol II subunit B9, C-terminal zinc ribbon / TATA-box binding protein, eukaryotic / Winged helix DNA-binding domain superfamily / RNA polymerase, RBP11-like subunit / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb7-like, N-terminal domain superfamily / Cyclin-like superfamily / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase RBP11 / RNA polymerase Rpb1, funnel domain superfamily / Rpb4/RPC9 superfamily / RNA polymerase Rpb1, domain 7 superfamily / DNA-directed RNA polymerase subunit Rpb5-like / TATA-box binding protein, conserved site / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / DNA-directed RNA polymerase, subunit RPB6 / RNA polymerase subunit RPB10 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / TFIIF beta subunit, HTH domain / TFIIF, beta subunit, N-terminal / RNA polymerase Rpb1, domain 3 superfamily / RPB5-like RNA polymerase subunit superfamily
DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIF subunit alpha / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerase II subunit RPB7 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor IIA large subunit / Transcription initiation factor IIB / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 ...DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIF subunit alpha / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerase II subunit RPB7 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor IIA large subunit / Transcription initiation factor IIB / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerase II subunit RPB4 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerase II subunit RPB1 / Transcription initiation factor IIF subunit beta
Biological speciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Baker's yeast (baker's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.1 Å
AuthorsDienemann C / Schwalb B / Schilbach S / Cramer P
Funding support Germany, 2 items
OrganizationGrant numberCountry
European Research Council693023 Germany
German Research FoundationSFB860 Germany
CitationJournal: Mol. Cell / Year: 2019
Title: Promoter Distortion and Opening in the RNA Polymerase II Cleft.
Authors: Christian Dienemann / Björn Schwalb / Sandra Schilbach / Patrick Cramer /
Abstract: Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron ...Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron microscopic (cryo-EM) structure of a yeast PIC that contains underwound, distorted promoter DNA in the closed Pol II cleft. The DNA duplex axis is offset at the upstream edge of the initially melted DNA region (IMR) where DNA opening begins. Unstable IMRs are found in a subset of yeast promoters that we show can still initiate transcription after depletion of the transcription factor (TF) IIH (TFIIH) translocase Ssl2 (XPB in human) from the nucleus in vivo. PIC-induced DNA distortions may thus prime the IMR for melting and may explain how unstable IMRs that are predicted in promoters of Pol I and Pol III can open spontaneously. These results suggest that DNA distortion in the polymerase cleft is a general mechanism that contributes to promoter opening.
Validation ReportPDB-ID: 6gyk

SummaryFull reportAbout validation report
History
DepositionJun 30, 2018-
Header (metadata) releaseAug 22, 2018-
Map releaseDec 5, 2018-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.003
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.003
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6gyk
  • Surface level: 0.003
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_0090.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 300 pix.
= 315. Å
1.05 Å/pix.
x 300 pix.
= 315. Å
1.05 Å/pix.
x 300 pix.
= 315. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.05 Å
Density
Contour LevelBy EMDB: 0.003 / Movie #1: 0.003
Minimum - Maximum-0.009033316 - 0.016516251
Average (Standard dev.)-0.00003435172 (±0.00065812847)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 315.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.051.051.05
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z315.000315.000315.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ450450450
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.0090.017-0.000

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Supplemental data

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Sample components

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Entire Yeast pre-initiation complex

EntireName: Yeast pre-initiation complexPreinitiation complex / Number of components: 26

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Component #1: protein, Yeast pre-initiation complex

ProteinName: Yeast pre-initiation complexPreinitiation complex / Recombinant expression: No

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Component #2: protein, RNA polymerase

ProteinName: RNA polymerase / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Component #3: protein, TFs

ProteinName: TFs / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #4: protein, Nucleic acids

ProteinName: Nucleic acidsNucleic acid / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: synthetic construct (others)

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Component #5: protein, DNA-directed RNA polymerase II subunit RPB1

ProteinName: DNA-directed RNA polymerase II subunit RPB1Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 191.821578 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #6: protein, DNA-directed RNA polymerase II subunit RPB2

ProteinName: DNA-directed RNA polymerase II subunit RPB2Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 138.937297 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #7: protein, DNA-directed RNA polymerase II subunit RPB3

ProteinName: DNA-directed RNA polymerase II subunit RPB3Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.330457 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #8: protein, DNA-directed RNA polymerase II subunit RPB4

ProteinName: DNA-directed RNA polymerase II subunit RPB4Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.319998 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #9: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC1RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.117094 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #10: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC2

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC2RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.800637 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #11: protein, DNA-directed RNA polymerase II subunit RPB7

ProteinName: DNA-directed RNA polymerase II subunit RPB7Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.081053 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #12: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC3

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.525363 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #13: protein, DNA-directed RNA polymerase II subunit RPB9

ProteinName: DNA-directed RNA polymerase II subunit RPB9Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.308161 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #14: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC5

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.290732 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #15: protein, DNA-directed RNA polymerase II subunit RPB11

ProteinName: DNA-directed RNA polymerase II subunit RPB11Polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.633493 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #16: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC4

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC4RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.729969 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #17: protein, Transcription initiation factor IIB

ProteinName: Transcription initiation factor IIB / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 38.25734 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #18: nucleic-acid, GAT1 Promoter

nucleic acidName: GAT1 Promoter / Class: DNA / Structure: OTHER / Synthetic: No
Sequence: (DT)(DG)(DC)(DC)(DC)(DG)(DG)(DC)(DC)(DC) (DA)(DG)(DC)(DC)(DA)(DC)(DA)(DT)(DA)(DT) (DA)(DT)(DA)(DT)(DA)(DG)(DG)(DT)(DG)(DT) (DG)(DT)(DG)(DC)(DC)(DA)(DC)(DT)(DC)(DC) (DC)(DG)(DG)(DC)(DC)(DA) ...Sequence:
(DT)(DG)(DC)(DC)(DC)(DG)(DG)(DC)(DC)(DC) (DA)(DG)(DC)(DC)(DA)(DC)(DA)(DT)(DA)(DT) (DA)(DT)(DA)(DT)(DA)(DG)(DG)(DT)(DG)(DT) (DG)(DT)(DG)(DC)(DC)(DA)(DC)(DT)(DC)(DC) (DC)(DG)(DG)(DC)(DC)(DA)(DC)(DG)(DG)(DT) (DA)(DT)(DT)(DA)(DG)(DC)
MassTheoretical: 17.153965 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Component #19: protein, TATA-box-binding protein

ProteinName: TATA-box-binding protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.042275 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #20: protein, Transcription initiation factor IIF subunit alpha

ProteinName: Transcription initiation factor IIF subunit alpha / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 82.32057 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #21: protein, Transcription initiation factor IIF subunit beta

ProteinName: Transcription initiation factor IIF subunit beta / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 46.684492 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #22: nucleic-acid, GAT1 promoter DNA

nucleic acidName: GAT1 promoter DNA / Class: DNA / Structure: OTHER / Synthetic: No
Sequence: (DG)(DC)(DT)(DA)(DA)(DT)(DA)(DC)(DC)(DG) (DT)(DG)(DG)(DC)(DC)(DG)(DG)(DG)(DA)(DG) (DT)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA)(DC) (DC)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT) (DG)(DT)(DG)(DG)(DC)(DT) ...Sequence:
(DG)(DC)(DT)(DA)(DA)(DT)(DA)(DC)(DC)(DG) (DT)(DG)(DG)(DC)(DC)(DG)(DG)(DG)(DA)(DG) (DT)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA)(DC) (DC)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT) (DG)(DT)(DG)(DG)(DC)(DT)(DG)(DG)(DG)(DC) (DC)(DG)(DG)(DG)(DC)(DA)
MassTheoretical: 17.3631 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Component #23: protein, Transcription initiation factor IIA large subunit,Transc...

ProteinName: Transcription initiation factor IIA large subunit,Transcription initiation factor IIA large subunit
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.432029 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #24: protein, Transcription initiation factor IIA subunit 2

ProteinName: Transcription initiation factor IIA subunit 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.431131 kDa
SourceSpecies: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #25: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 9 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Component #26: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 37 e/Å2 / Illumination mode: OTHER
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 QUANTUM (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 39000
3D reconstructionResolution: 5.1 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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