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- EMDB-4984: RNA Polymerase I Closed Conformation 2 (CC2) -

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Basic information

Entry
Database: EMDB / ID: EMD-4984
TitleRNA Polymerase I Closed Conformation 2 (CC2)
Map data
SampleRNA Polymerase I Closed Complex conformation 2 (CC2)
  • Nucleic acid
  • transcription initiation factor
  • RNA Polymerase I
  • (nucleic-acidNucleic acid) x 2
  • (RNA polymerase I-specific transcription initiation factor ...) x 4
  • (DNA-directed RNA polymerase I subunit ...Polymerase) x 7
  • (DNA-directed RNA polymerases I and III subunit ...) x 2
  • (DNA-directed RNA polymerases I, II, and III subunit ...RNA polymerase) x 5
Function / homology
Function and homology information


RNA polymerase I core factor complex / RNA polymerase transcription factor SL1 complex / RNA polymerase I transcription regulatory region sequence-specific DNA binding / RNA polymerase I core promoter sequence-specific DNA binding / RNA polymerase I core binding / RNA polymerase I general transcription initiation factor binding / rDNA binding / RNA polymerase I general transcription initiation factor activity / transcription elongation from RNA polymerase I promoter / nucleolar large rRNA transcription by RNA polymerase I ...RNA polymerase I core factor complex / RNA polymerase transcription factor SL1 complex / RNA polymerase I transcription regulatory region sequence-specific DNA binding / RNA polymerase I core promoter sequence-specific DNA binding / RNA polymerase I core binding / RNA polymerase I general transcription initiation factor binding / rDNA binding / RNA polymerase I general transcription initiation factor activity / transcription elongation from RNA polymerase I promoter / nucleolar large rRNA transcription by RNA polymerase I / transposon integration / RNA polymerase II-specific DNA-binding transcription factor binding / RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / transcription initiation from RNA polymerase I promoter / termination of RNA polymerase I transcription / RNA Polymerase I Transcription Initiation / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 1 Promoter / termination of RNA polymerase III transcription / regulation of cell size / Formation of TC-NER Pre-Incision Complex / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Initiation And Promoter Clearance / TP53 Regulates Transcription of DNA Repair Genes / RNA polymerase I activity / Gap-filling DNA repair synthesis and ligation in TC-NER / Dual incision in TC-NER / RNA Polymerase II Transcription Elongation / tRNA transcription by RNA polymerase III / RNA Polymerase I Promoter Escape / Estrogen-dependent gene expression / RNA Polymerase II Pre-transcription Events / transcription by RNA polymerase I / mRNA cleavage / RNA polymerase I complex / RNA polymerase III complex / transcription by RNA polymerase III / RNA polymerase II, core complex / TBP-class protein binding / promoter-specific chromatin binding / ribonucleoside binding / DNA-directed RNA polymerase / transcription by RNA polymerase II / transcription, RNA-templated / ribosome biogenesis / nucleic acid binding / protein dimerization activity / nucleolus / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
Similarity search - Function
RNA polymerase I specific transcription initiation factor RRN3 / RNA polymerase I specific transcription initiation factor RRN3 / Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 / RNA polymerase I-specific transcription initiation factor Rrn6 / Transcription initiation factor Rrn11, budding yeast / RNA polymerase I-specific transcription initiation factor RRN6-like / Transcription initiation factor Rrn7, Zinc-finger / RNA polymerase I transcription initiation factor TAF1B/Rrn7 / Transcription initiation factor Rrn11 / RNA polymerase I-specific transcription-initiation factor ...RNA polymerase I specific transcription initiation factor RRN3 / RNA polymerase I specific transcription initiation factor RRN3 / Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 / RNA polymerase I-specific transcription initiation factor Rrn6 / Transcription initiation factor Rrn11, budding yeast / RNA polymerase I-specific transcription initiation factor RRN6-like / Transcription initiation factor Rrn7, Zinc-finger / RNA polymerase I transcription initiation factor TAF1B/Rrn7 / Transcription initiation factor Rrn11 / RNA polymerase I-specific transcription-initiation factor / RNA polymerase I specific initiation factor / DNA-directed RNA polymerase I subunit RPA34.5 / DNA-directed RNA polymerase I, subunit RPA34.5 / RNA polymerase I associated factor, A49-like / A49-like RNA polymerase I associated factor / RNA polymerase I, subunit Rpa14, fungi / Yeast RNA polymerase I subunit RPA14 / Rpa43, N-terminal ribonucleoprotein (RNP) domain / DNA-directed RNA polymerase I subunit RPA2, domain 4 / DNA-directed RNA pol I, largest subunit / Pol I subunit A12, C-terminal zinc ribbon / RNA polymerase I, Rpa2 specific domain / RPA43 OB domain in RNA Pol I / RPA43, OB domain / DNA-directed RNA polymerases I and III subunit AC19 / DNA-directed RNA polymerases I and III subunit AC40 / RNA polymerase Rpb7-like , N-terminal / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / Zinc finger TFIIS-type signature. / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerases, subunit N, zinc binding site / DNA-directed RNA polymerase, subunit N/Rpb10 / RNA polymerases N / 8 kDa subunit / RNA polymerase subunit RPB10 / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase, Rpb5, N-terminal / DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus / DNA-directed RNA polymerase subunit/transcription factor S / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase Rpb5, N-terminal domain / RNA polymerase subunit 9 / RNA polymerase, Rpb8 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / DNA-directed RNA polymerase, subunit RPB6 / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase subunit CX / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / Zinc finger, TFIIS-type / C2C2 Zinc finger / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb5, C-terminal domain / Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit / DNA-directed RNA polymerase subunit Rpb5-like / RNA polymerases L / 13 to 16 Kd subunits signature. / RNA polymerase Rpb3/Rpb11 dimerisation domain / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / RPB5-like RNA polymerase subunit superfamily / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase, subunit H/Rpb5 C-terminal / RNA polymerase, subunit H/Rpb5, conserved site / Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit / RNA polymerases K / 14 to 18 Kd subunits signature. / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / RNA polymerase Rpb6 / RNA polymerase subunit, RPB6/omega / RNA polymerase, subunit omega/K/RPB6 / RNA polymerase Rpb6 / RPB6/omega subunit-like superfamily / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 5 / RNA polymerase, N-terminal / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 2 / RNA polymerase beta subunit / RNA polymerase, beta subunit, protrusion / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 superfamily / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase Rpb3/Rpb11 dimerisation domain
Similarity search - Domain/homology
RNA polymerase I-specific transcription initiation factor RRN6 / DNA-directed RNA polymerase I subunit RPA49 / DNA-directed RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I subunit RPA34 / DNA-directed RNA polymerase I subunit RPA43 / RNA polymerase I-specific transcription initiation factor RRN7 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase I-specific transcription initiation factor RRN3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerase I subunit RPA12 ...RNA polymerase I-specific transcription initiation factor RRN6 / DNA-directed RNA polymerase I subunit RPA49 / DNA-directed RNA polymerase I subunit RPA14 / DNA-directed RNA polymerase I subunit RPA34 / DNA-directed RNA polymerase I subunit RPA43 / RNA polymerase I-specific transcription initiation factor RRN7 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase I-specific transcription initiation factor RRN3 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerase I subunit RPA12 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase I subunit RPA135 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase I subunit RPA190 / DNA-directed RNA polymerases I and III subunit RPAC1 / RNA polymerase I-specific transcription initiation factor RRN11
Similarity search - Component
Biological speciessynthetic construct (others) / Saccharomyces cerevisiae (baker's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsMueller CW / Sadian Y / Tafur L
Funding support1 items
OrganizationGrant numberCountry
European Research CouncilERC-2013-AdG340964-POL1PIC
CitationJournal: Nat Commun / Year: 2019
Title: Molecular insight into RNA polymerase I promoter recognition and promoter melting.
Authors: Yashar Sadian / Florence Baudin / Lucas Tafur / Brice Murciano / Rene Wetzel / Felix Weis / Christoph W Müller /
Abstract: RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I ...RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.
History
DepositionMay 16, 2019-
Header (metadata) releaseDec 11, 2019-
Map releaseDec 11, 2019-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6rql
  • Surface level: 0.04
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_4984.map.gz / Format: CCP4 / Size: 209.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 380 pix.
= 395.2 Å
1.04 Å/pix.
x 380 pix.
= 395.2 Å
1.04 Å/pix.
x 380 pix.
= 395.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum-0.17502543 - 0.27431312
Average (Standard dev.)0.00026258786 (±0.005862969)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions380380380
Spacing380380380
CellA=B=C: 395.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.041.041.04
M x/y/z380380380
origin x/y/z0.0000.0000.000
length x/y/z395.200395.200395.200
α/β/γ90.00090.00090.000
start NX/NY/NZ-51-35-11
NX/NY/NZ11110799
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS380380380
D min/max/mean-0.1750.2740.000

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Supplemental data

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Sample components

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Entire RNA Polymerase I Closed Complex conformation 2 (CC2)

EntireName: RNA Polymerase I Closed Complex conformation 2 (CC2) / Number of Components: 24

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Component #1: protein, RNA Polymerase I Closed Complex conformation 2 (CC2)

ProteinName: RNA Polymerase I Closed Complex conformation 2 (CC2) / Recombinant expression: No

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Component #2: protein, Nucleic acid

ProteinName: Nucleic acid / Recombinant expression: No
SourceSpecies: synthetic construct (others)
Source (engineered)Expression System: synthetic construct (others)

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Component #3: protein, transcription initiation factor

ProteinName: transcription initiation factor / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #4: protein, RNA Polymerase I

ProteinName: RNA Polymerase I / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #5: nucleic-acid, Template strand

nucleic acidName: Template strandTranscription (biology) / Class: DNA / Structure: OTHER / Synthetic: No
Sequence: (DG)(DT)(DC)(DT)(DT)(DC)(DA)(DA)(DC)(DT) (DG)(DC)(DT)(DT)(DT)(DC)(DG)(DC)(DA)(DT) (DG)(DA)(DA)(DG)(DT)(DA)(DC)(DC)(DT)(DC) (DC)(DC)(DA)(DA)(DC)(DT)(DA)(DC)(DT)(DT) (DT)(DT)(DC)(DC)(DT)(DC) ...Sequence:
(DG)(DT)(DC)(DT)(DT)(DC)(DA)(DA)(DC)(DT) (DG)(DC)(DT)(DT)(DT)(DC)(DG)(DC)(DA)(DT) (DG)(DA)(DA)(DG)(DT)(DA)(DC)(DC)(DT)(DC) (DC)(DC)(DA)(DA)(DC)(DT)(DA)(DC)(DT)(DT) (DT)(DT)(DC)(DC)(DT)(DC)(DA)(DC)(DA)(DC) (DT)(DT)(DG)(DT)(DA)(DC)(DT)(DC)(DC)(DA) (DT)(DG)(DA)(DC)(DT)(DA)(DA)(DA)(DC)(DC)
MassTheoretical: 21.216605 kDa
SourceSpecies: synthetic construct (others)

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Component #6: nucleic-acid, Nontemplate strand

nucleic acidName: Nontemplate strand / Class: DNA / Structure: OTHER / Synthetic: No
Sequence: (DG)(DG)(DT)(DT)(DT)(DA)(DG)(DT)(DC)(DA) (DT)(DG)(DG)(DA)(DG)(DT)(DA)(DC)(DA)(DA) (DG)(DT)(DG)(DT)(DG)(DA)(DG)(DG)(DA)(DA) (DA)(DA)(DG)(DT)(DA)(DG)(DT)(DT)(DG)(DG) (DG)(DA)(DG)(DG)(DT)(DA) ...Sequence:
(DG)(DG)(DT)(DT)(DT)(DA)(DG)(DT)(DC)(DA) (DT)(DG)(DG)(DA)(DG)(DT)(DA)(DC)(DA)(DA) (DG)(DT)(DG)(DT)(DG)(DA)(DG)(DG)(DA)(DA) (DA)(DA)(DG)(DT)(DA)(DG)(DT)(DT)(DG)(DG) (DG)(DA)(DG)(DG)(DT)(DA)(DC)(DT)(DT)(DC) (DA)(DT)(DG)(DC)(DG)(DA)(DA)(DA)(DG)(DC) (DA)(DG)(DT)(DT)(DG)(DA)(DA)(DG)(DA)(DC)
MassTheoretical: 21.942078 kDa
SourceSpecies: synthetic construct (others)

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Component #7: protein, RNA polymerase I-specific transcription initiation facto...

ProteinName: RNA polymerase I-specific transcription initiation factor RRN7
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 60.435195 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #8: protein, RNA polymerase I-specific transcription initiation facto...

ProteinName: RNA polymerase I-specific transcription initiation factor RRN6
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 102.163227 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #9: protein, RNA polymerase I-specific transcription initiation facto...

ProteinName: RNA polymerase I-specific transcription initiation factor RRN11
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 59.334262 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #10: protein, DNA-directed RNA polymerase I subunit RPA49

ProteinName: DNA-directed RNA polymerase I subunit RPA49Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 46.721707 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #11: protein, DNA-directed RNA polymerase I subunit RPA190

ProteinName: DNA-directed RNA polymerase I subunit RPA190Polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 186.676969 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #12: protein, DNA-directed RNA polymerase I subunit RPA135

ProteinName: DNA-directed RNA polymerase I subunit RPA135Polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 135.910328 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #13: protein, DNA-directed RNA polymerases I and III subunit RPAC1

ProteinName: DNA-directed RNA polymerases I and III subunit RPAC1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 37.732613 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #14: protein, DNA-directed RNA polymerase I subunit RPA14

ProteinName: DNA-directed RNA polymerase I subunit RPA14Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.599128 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #15: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC1RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.117094 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #16: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC2

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC2RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.931834 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #17: protein, DNA-directed RNA polymerase I subunit RPA43

ProteinName: DNA-directed RNA polymerase I subunit RPA43Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 36.264852 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #18: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC3

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.525363 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #19: protein, DNA-directed RNA polymerase I subunit RPA12

ProteinName: DNA-directed RNA polymerase I subunit RPA12Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.676566 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #20: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC5

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.290732 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #21: protein, DNA-directed RNA polymerases I and III subunit RPAC2

ProteinName: DNA-directed RNA polymerases I and III subunit RPAC2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.16786 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #22: protein, DNA-directed RNA polymerases I, II, and III subunit RPABC4

ProteinName: DNA-directed RNA polymerases I, II, and III subunit RPABC4RNA polymerase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.729969 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #23: protein, DNA-directed RNA polymerase I subunit RPA34

ProteinName: DNA-directed RNA polymerase I subunit RPA34Polymerase / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.933518 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Saccharomyces cerevisiae (baker's yeast)

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Component #24: protein, RNA polymerase I-specific transcription initiation facto...

ProteinName: RNA polymerase I-specific transcription initiation factor RRN3
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 72.458258 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Experimental details

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Sample preparation

SpecimenSpecimen State: Particle / Method: cryo EM
Sample solutionpH: 7.5
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen Name: ETHANE / Temperature: 4 K / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron Source: FIELD EMISSION GUN / Accelerating Voltage: 300 kV / Electron Dose: 1.1075 e/Å2 / Illumination Mode: FLOOD BEAM
LensImaging Mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of Projections: 24482
3D reconstructionResolution: 2.9 Å / Resolution Method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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