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- SASDA32: BSA in HEPES (Serum albumin, BSA) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDA32
SampleBSA in HEPES
  • Serum albumin (protein), BSA, Bos taurus
Function / homology
Function and homology information


enterobactin binding / cellular response to calcium ion starvation / negative regulation of mitochondrial depolarization / toxic substance binding / cellular response to starvation / fatty acid binding / pyridoxal phosphate binding / protein-containing complex / DNA binding / extracellular space ...enterobactin binding / cellular response to calcium ion starvation / negative regulation of mitochondrial depolarization / toxic substance binding / cellular response to starvation / fatty acid binding / pyridoxal phosphate binding / protein-containing complex / DNA binding / extracellular space / extracellular region / metal ion binding / cytoplasm
Similarity search - Function
Serum albumin/Alpha-fetoprotein/Afamin / ALB/AFP/VDB / Serum albumin, N-terminal / Serum albumin, conserved site / Serum albumin-like / Serum albumin family / Albumin domain signature. / Albumin domain profile. / serum albumin
Similarity search - Domain/homology
Biological speciesBos taurus (cattle)
Contact author
  • Darja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #4
Type: dummy / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 2.566404
Search similar-shape structures of this assembly by Omokage search (details)
Model #3
Type: atomic / Software: (28)
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: BSA in HEPES / Ext coefficient: 6.46 / Contrast: 2.953 / Specific vol: 0.737 / Purity method: gel filtration / Dry vol: 17570 / Sample MW: 66 kDa / Specimen concentration: 25.64 mg/ml / Concentration method: nanodrop
BufferName: 50 mM HEPES 50 mM KCl / Concentration: 50.00 mM / PK: 7 / pH: 7.5 / Comment: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid / Composition: KCl 50.000 mM
Entity #1Name: BSA / Type: protein / Description: Serum albumin / Formula weight: 69.29 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P02769
Sequence: MKWVTFISLL LLFSSAYSRG VFRRDTHKSE IAHRFKDLGE EHFKGLVLIA FSQYLQQCPF DEHVKLVNEL TEFAKTCVAD ESHAGCEKSL HTLFGDELCK VASLRETYGD MADCCEKQEP ERNECFLSHK DDSPDLPKLK PDPNTLCDEF KADEKKFWGK YLYEIARRHP ...Sequence:
MKWVTFISLL LLFSSAYSRG VFRRDTHKSE IAHRFKDLGE EHFKGLVLIA FSQYLQQCPF DEHVKLVNEL TEFAKTCVAD ESHAGCEKSL HTLFGDELCK VASLRETYGD MADCCEKQEP ERNECFLSHK DDSPDLPKLK PDPNTLCDEF KADEKKFWGK YLYEIARRHP YFYAPELLYY ANKYNGVFQE CCQAEDKGAC LLPKIETMRE KVLASSARQR LRCASIQKFG ERALKAWSVA RLSQKFPKAE FVEVTKLVTD LTKVHKECCH GDLLECADDR ADLAKYICDN QDTISSKLKE CCDKPLLEKS HCIAEVEKDA IPENLPPLTA DFAEDKDVCK NYQEAKDAFL GSFLYEYSRR HPEYAVSVLL RLAKEYEATL EECCAKDDPH ACYSTVFDKL KHLVDEPQNL IKQNCDQFEK LGEYGFQNAL IVRYTRKVPQ VSTPTLVEVS RSLGKVGTRC CTKPESERMP CTEDYLSLIL NRLCVLHEKT PVSEKVTKCC TESLVNRRPC FSALTPDETY VPKAFDEKLF TFHADICTLP DTEKQIKKQT ALVELLKHKP KATEEQLKTV MENFVAFVDK CCAADDKEAC FAVEGPKLVV STQTALA

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Experimental information

BeamInstrument name: DORIS III EMBL X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Title: BSA in Hepes / Measurement date: Sep 12, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 8 / Unit: 1/nm /
MinMax
Q0.0871 6.0145
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 474 /
MinMax
Q0.1432 2.732
P(R) point21 494
R0 8.698
Result
Type of curve: single_conc / Standard: BSA
Comments: Bovin Serum Albumin (BSA) is an extremely important protein in Small Angle X-Ray Scattering. It is, in fact, used as standard in order to calculate the molecular weight of other proteins. ...Comments: Bovin Serum Albumin (BSA) is an extremely important protein in Small Angle X-Ray Scattering. It is, in fact, used as standard in order to calculate the molecular weight of other proteins. The reasons why it has been chosen as standard it is because it can be easily stored in its powder form and it is also very well characterised. In solution is present as a monomer-dimer equilibrium, therefore the molecular weight of the standard BSA is not the one of the monomer (68 kDa) but is slightly bigger (72 kDa). The crystal structure of BSA has been solved recently therefore ab initio models can be compared with atomic structure like in this picture.
ExperimentalStandardPorod
MW64 kDa64 kDa-
Volume--97.1 nm3

P(R)Guinier
Forward scattering, I058.1 59.3
Radius of gyration, Rg2.81 nm2.92 nm

MinMax
D-9.3
Guinier point19 115

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