+Open data
-Basic information
Entry | Database: PDB / ID: 7bou | |||||||||
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Title | GP8 of Mature Bacteriophage T7 | |||||||||
Components | Portal protein | |||||||||
Keywords | STRUCTURAL PROTEIN / Portal / Bacteriophage / Mature / Transport | |||||||||
Function / homology | Portal protein, Caudovirales / Head-to-tail connector protein, podovirus-type / Bacteriophage head to tail connecting protein / viral portal complex / symbiont genome ejection through host cell envelope, short tail mechanism / viral DNA genome packaging / Portal protein Function and homology information | |||||||||
Biological species | Escherichia phage T7 (virus) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / Resolution: 3.6 Å | |||||||||
Authors | Chen, W.Y. / Xiao, H. | |||||||||
Funding support | China, 2items
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Citation | Journal: Protein Cell / Year: 2020 Title: Structural changes of a bacteriophage upon DNA packaging and maturation. Authors: Wenyuan Chen / Hao Xiao / Xurong Wang / Shuanglin Song / Zhen Han / Xiaowu Li / Fan Yang / Li Wang / Jingdong Song / Hongrong Liu / Lingpeng Cheng / | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7bou.cif.gz | 1019.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7bou.ent.gz | 870.5 KB | Display | PDB format |
PDBx/mmJSON format | 7bou.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bo/7bou ftp://data.pdbj.org/pub/pdb/validation_reports/bo/7bou | HTTPS FTP |
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-Related structure data
Related structure data | 30134MC 7boxC 7boyC 7bozC 7bp0C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 59173.984 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia phage T7 (virus) / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P03728 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Mature GP8 / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Escherichia phage T7 (virus) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: NO |
-Electron microscopy imaging
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TECNAI ARCTICA |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / C2 aperture diameter: 70 µm |
Image recording | Electron dose: 24 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 50000 / Symmetry type: POINT |