3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / nuclear pore inner ring / nuclear pore organization / ATPase inhibitor activity / mitochondrial genome maintenance / ribonuclease III activity / negative regulation of ATP-dependent activity / fungal-type vacuole / DNA strand exchange activity ...3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / nuclear pore inner ring / nuclear pore organization / ATPase inhibitor activity / mitochondrial genome maintenance / ribonuclease III activity / negative regulation of ATP-dependent activity / fungal-type vacuole / DNA strand exchange activity / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / mitochondrial small ribosomal subunit / structural constituent of nuclear pore / mitochondrial translation / superoxide dismutase activity / 転写後修飾 / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / ribosomal small subunit assembly / double-stranded RNA binding / small ribosomal subunit rRNA binding / single-stranded DNA binding / large ribosomal subunit rRNA binding / small ribosomal subunit / cytosolic small ribosomal subunit / transferase activity / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / リボソーム / structural constituent of ribosome / ribonucleoprotein complex / 翻訳 (生物学) / mRNA binding / ミトコンドリア / RNA binding / metal ion binding / 細胞質 類似検索 - 分子機能
54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain ...54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 / 54S ribosomal protein L28, mitochondrial / Ribosomal protein L31, mitochondrial / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein L31 / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / IGR protein motif / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / Ribonuclease-III-like / IGR protein motif / IGR / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / 28S ribosomal protein S25, mitochondrial / Ribonuclease III family domain profile. / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L37 / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Ribonuclease III family / Ribonuclease III domain / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Ribosomal protein L49/IMG2 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial large subunit ribosomal protein (Img2) / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Enoyl-CoA hydratase/isomerase, conserved site / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / Enoyl-CoA hydratase/isomerase signature. / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Double-stranded RNA binding motif / Double-stranded RNA binding motif / Ribonuclease III, endonuclease domain superfamily / Double-stranded RNA-binding domain / : / NUDIX domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX hydrolase domain / ClpP/crotonase-like domain superfamily / Ribosomal protein L1, 3-layer alpha/beta-sandwich / NUDIX hydrolase-like domain superfamily / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L11, bacterial-type / Ribosomal protein S21 / Sterile alpha motif/pointed domain superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L10-like domain superfamily / : 類似検索 - ドメイン・相同性
ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / スペルミン / : / リボ核酸 / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Uncharacterized protein ...ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / スペルミン / : / リボ核酸 / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Uncharacterized protein / 37S ribosomal protein mrp5 / Large ribosomal subunit protein uL16m / Ribosomal protein S16, mitochondrial / Ribosomal protein S5, mitochondrial / Large ribosomal subunit protein bL32m / 37S ribosomal protein S5 / Ribosomal protein S15 / Mitochondrial 37S ribosomal protein S27 / 37S ribosomal protein S24, mitochondrial / Large ribosomal subunit protein uL1m / Large ribosomal subunit protein uL5m / Protein FYV4, mitochondrial / Large ribosomal subunit protein mL58 / Large ribosomal subunit protein bL27m / Mitochondrial 3-hydroxyisobutyryl-CoA hydrolase / Large ribosomal subunit protein bL31m / Large ribosomal subunit protein bL34m / Large ribosomal subunit protein bL17m / Mito ribosomal protein S18 / Large ribosomal subunit protein uL3m / Mitochondrial ribosomal protein S19 / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein mL61 / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein mL38 / 37S ribosomal protein S10, mitochondrial / Large ribosomal subunit protein mL67 / Small ribosomal subunit protein mS27 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein uL6m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein mL57 / Large ribosomal subunit protein mL46 / 40S ribosomal protein S13 / Fe superoxide dismutase / Large ribosomal subunit protein mL43 / Mitochondrial 37S ribosomal protein S17 / Large ribosomal subunit protein bL36m / 37S ribosomal protein mrp10, mitochondrial / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein mL41 / Ribosomal_S7 domain-containing protein / Large ribosomal subunit protein mL50 / 37S ribosomal protein S9, mitochondrial / Large ribosomal subunit protein uL29m / Mitochondrial 30S ribosomal protein S12 / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL44 / Mito ribosomal protein S4 / Mito ribosomal protein S21 / Mito ribosomal protein S6 / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein mL59 / Mitochondrial 40S ribosomal protein MRP2 / Protein FYV4, mitochondrial / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL53 / Mitochondrial ribosomal protein subunit S18 / Small ribosomal subunit protein mS45 / 40S ribosomal protein S8 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS23 / Large ribosomal subunit protein mL60 / Small ribosomal subunit protein uS2m / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL4m / ATP synthase F1 subunit epsilon 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.