- PDB-6hiw: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -
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Entry
Database: PDB / ID: 6hiw
Title
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3
modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / valine catabolic process / kinetoplast / thiosulfate sulfurtransferase activity / quorum sensing ...modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / valine catabolic process / kinetoplast / thiosulfate sulfurtransferase activity / quorum sensing / nuclear lumen / regulation of protein kinase A signaling / enoyl-CoA hydratase activity / mRNA stabilization / ciliary plasm / mitochondrial small ribosomal subunit / superoxide dismutase / fatty acid beta-oxidation / protein kinase A regulatory subunit binding / superoxide dismutase activity / RNA processing / mitochondrion organization / cytosolic small ribosomal subunit / hydrolase activity / ribosome / structural constituent of ribosome / translation / mRNA binding / mitochondrion / RNA binding / nucleoplasm / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function
Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / : / Probable Zinc-ribbon domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 ...Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / : / Probable Zinc-ribbon domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Cyclic phosphodiesterase / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Rhodanese-like domain / ClpP/crotonase-like domain superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ribosomal protein S5, C-terminal / Ubiquitin family / Ribosomal protein S5, C-terminal domain / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S9 / Ribosomal protein S9/S16 / Ubiquitin domain profile. / Ribosomal protein S17/S11 / Ribosomal protein S17 / Ubiquitin-like domain / Ribosomal protein S11 superfamily / S15/NS1, RNA-binding / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / SPERMIDINE / SPERMINE / URIDINE 5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) / A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein ...GUANOSINE-5'-TRIPHOSPHATE / SPERMIDINE / SPERMINE / URIDINE 5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) / A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Probable Zinc-ribbon domain-containing protein / 30S ribosomal protein S8, putative / Small ribosomal subunit protein mS41 / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Small ribosomal subunit protein uS5 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Trans-sialidase / Uncharacterized protein / 30S Ribosomal protein S17, putative / Uncharacterized protein / Uncharacterized protein / Opioid growth factor receptor (OGFr) conserved domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S23 mitochondrial conserved domain-containing protein / Uncharacterized protein / Ribosomal protein S9 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / LysM domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Ubiquitin-like domain-containing protein / Uncharacterized protein / Uncharacterized protein / Small ribosomal subunit protein mS29 / Manganese/iron superoxide dismutase C-terminal domain-containing protein / Superoxide dismutase, putative / Rhodanese domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein Similarity search - Component
Biological species
Trypanosoma brucei brucei (eukaryote)
Method
ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.37 Å
Journal: Science / Year: 2018 Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes. Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban / Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
Protein , 57 types, 57 molecules DADDDIDLDMDNDODPDQDRDSDUDZDaDBDCDEDFDGDHDJDKDTDVDWDXDYCCCECF...
#1: Protein
mS48
Mass: 201811.188 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57UJ2
#2: Protein
mS51
Mass: 95080.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q385L8
#3: Protein
mS56
Mass: 47306.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q587C2
#4: Protein
mS59
Mass: 35395.676 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38BS2
#5: Protein
mS60
Mass: 34546.641 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57XL2
#6: Protein
mS61
Mass: 33482.340 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38D60
#7: Protein
mS62
Mass: 32068.100 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q383D1
#8: Protein
mS63
Mass: 31922.178 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38F25
#9: Protein
mS64
Mass: 30742.207 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q382E8
#10: Protein
mS65
Mass: 30617.381 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57UA2
#11: Protein
mS66
Mass: 29960.182 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q388L7
#12: Protein
mS68
Mass: 26472.807 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q582T9
#13: Protein
mS73
Mass: 11360.885 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q587C4
#14: Protein
mS74
Mass: 8203.408 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#15: Protein
mS49
Mass: 137234.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q586P5
#16: Protein
mS50
Mass: 131719.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57YB5
#17: Protein
mS52
Mass: 85012.258 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q386Q7
#18: Protein
mS53
Mass: 75338.914 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38ET1
#19: Protein
mS54
Mass: 72046.031 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57ZP8
#20: Protein
mS55
Mass: 66676.680 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q580V1
#21: Protein
mS57
Mass: 45956.293 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q584U8
#22: Protein
mS58
Mass: 35623.910 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38BP1
#23: Protein
mS67
Mass: 30096.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q586G5
#24: Protein
mS69
Mass: 21589.732 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57UZ6
#25: Protein
mS70
Mass: 21247.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q383N9
#26: Protein
mS71
Mass: 20093.227 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q383G5
#27: Protein
mS72
Mass: 19317.293 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57YD4
#28: Protein
uS3m
Mass: 9070.940 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: mitochondrial gene for uS3m (MURF-5) / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#29: Protein
uS5m
Mass: 50147.355 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38AX6
#30: Protein
bS6m
Mass: 18861.543 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues 105-114 built as polyalanines / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38BW5
#31: Protein
uS8m
Mass: 32766.459 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q388R7
#32: Protein
uS9m
Mass: 50201.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57W62
#33: Protein
uS10m
Mass: 94351.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57Z45
#34: Protein
uS11m
Mass: 37925.910 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q389T7
#35: Protein
uS12m
Mass: 10421.635 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Mitochondrial gene subject to RNA editingalternative start codon Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#36: Protein
uS14m
Mass: 19900.014 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q580I0
#37: Protein
uS15m
Mass: 50401.191 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q4GZ99
#38: Protein
bS16m
Mass: 22077.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q384N9
#39: Protein
uS17m
Mass: 35632.727 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38DP8
#40: Protein
bS18m
Mass: 36988.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38AS2
#41: Protein
uS19m
Mass: 28364.604 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q584T8
#42: Protein
bS21m
Mass: 23087.889 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q580M9
#43: Protein
mt-IF-3
Mass: 41086.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues 294-304 built as polyalanines / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57WU2
#44: Protein
mS22
Mass: 73064.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues 20-37 built as polyalanines / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38DR3
#45: Protein
mS23
Mass: 36855.578 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57VB2
#46: Protein
mS26
Mass: 51164.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues 221-248 built as polyalanines / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38DK6
#47: Protein
mS29
Mass: 57048.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q585C2
#48: Protein
mS33
Mass: 21244.104 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57WW0
#49: Protein
mS34
Mass: 28728.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57UK0
#50: Protein
mS35
Mass: 98377.773 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q387C7
#51: Protein
mS37
Mass: 24757.896 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q38C96
#52: Protein
mS38
Mass: 28240.600 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q57VQ9
#53: Protein
mS41
Mass: 22421.469 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q389L3
#54: Protein
mS42
Mass: 29748.666 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q586A1, superoxide dismutase
#55: Protein
mS43
Mass: 48590.410 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues 27-35 built as polyalanines / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q585I1
#56: Protein
mS47
Mass: 137928.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: UniProt: Q383R4
#62: Protein
UnknownProtein
Mass: 4613.678 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
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RNA chain , 1 types, 1 molecules CA
#57: RNA chain
9SrRNA
Mass: 198442.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: post-transcriptional addition of 7 U residues at the 3' end Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427 / References: GenBank: 343546
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Protein/peptide , 5 types, 5 molecules UOUPUQURUT
#58: Protein/peptide
UnknownProtein
Mass: 443.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues built as UNK / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#59: Protein/peptide
UnknownProtein
Mass: 613.749 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues built as UNK / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#60: Protein/peptide
UnknownProtein
Mass: 2741.370 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues built as UNK / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#61: Protein/peptide
UnknownProtein
Mass: 698.854 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: residues built as UNK / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
#63: Protein/peptide
UnknownProtein
Mass: 3762.629 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Variant: Lister 427
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Vitrification
Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE-PROPANE / Humidity: 98 % / Chamber temperature: 278 K
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Electron microscopy imaging
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
Microscopy
Model: FEI TITAN KRIOS
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lens
Mode: BRIGHT FIELD
Image recording
Electron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)
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Processing
EM software
ID
Name
Version
Category
1
EMAN
particleselection
4
CTFFIND
CTFcorrection
10
RELION
2
finalEulerassignment
11
RELION
2
classification
13
Coot
modelrefinement
14
PHENIX
modelrefinement
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstruction
Resolution: 3.37 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31911 / Algorithm: FOURIER SPACE / Num. of class averages: 1 / Symmetry type: POINT
Atomic model building
B value: 46.4 / Protocol: AB INITIO MODEL Details: We used Coot and O for initial model building and refined the structure using PHENIX
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