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Structure paper

TitleEvolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Journal, issue, pagesScience, Vol. 362, Issue 6413, Year 2018
Publish dateOct 26, 2018
AuthorsDavid J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
PubMed AbstractRibosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
External linksScience / PubMed:30213880
MethodsEM (single particle)
Resolution3.08 - 7.8 Å
Structure data

0229

6hiv

EMDB-0229, PDB-6hiv:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome
Method: EM (single particle) / Resolution: 7.8 Å

0230

6hiw

EMDB-0230, PDB-6hiw:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3
Method: EM (single particle) / Resolution: 3.37 Å

0231

6hix

EMDB-0231, PDB-6hix:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit
Method: EM (single particle) / Resolution: 3.39 Å

0232

6hiy

EMDB-0232, PDB-6hiy:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the body of the small mitoribosomal subunit in complex with mt-IF-3
Method: EM (single particle) / Resolution: 3.27 Å

0233

6hiz

EMDB-0233, PDB-6hiz:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the head of the small mitoribosomal subunit
Method: EM (single particle) / Resolution: 3.08 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SPD:
SPERMIDINE / Spermidine

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-SPM:
SPERMINE / Spermine

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

ChemComp-SER:
SERINE / Serine

ChemComp-HOH:
WATER / Water

Source
  • trypanosoma brucei brucei (eukaryote)
KeywordsRIBOSOME / mitoribosome / translation / Trypanosoma / large ribosomal subunit / small ribosomal subunit / 12S rRNA / 9S rRNA / ribosomal protein / mitochondrial initiation factor IF-3

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