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- PDB-6hiv: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -

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Basic information

Entry
Database: PDB / ID: 6hiv
TitleCryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome
Components
  • (50S ribosomal protein ...) x 3
  • (UNK) x 17
  • (bL12m) x 3
  • (mL68) x 2
  • 12S rRNAMT-RNR1
  • 9s rRNA
  • bL19m
  • bL20m
  • bL21m
  • bL27m
  • bL28m
  • bL31m
  • bL32m
  • bL33m
  • bL35m
  • bL36m
  • bL9m
  • bS12m
  • bS16m
  • bS18m
  • bS6m
  • mL100
  • mL38
  • mL40
  • mL41
  • mL42
  • mL43
  • mL46
  • mL49
  • mL52
  • mL53
  • mL63
  • mL67
  • mL69
  • mL70
  • mL71
  • mL72
  • mL73
  • mL74
  • mL75
  • mL76
  • mL77
  • mL78
  • mL79
  • mL80
  • mL81
  • mL82
  • mL83
  • mL84
  • mL85
  • mL86
  • mL87
  • mL88
  • mL89
  • mL90
  • mL92
  • mL93
  • mL94
  • mL95
  • mL96
  • mL97
  • mL98
  • mL99
  • mS22
  • mS23
  • mS26
  • mS29
  • mS33
  • mS34
  • mS35
  • mS37
  • mS38
  • mS43
  • mS47
  • mS52
  • mS91
  • ms34
  • ms42
  • ms48
  • ms49
  • ms50
  • ms51
  • ms53
  • ms54
  • ms55
  • ms56
  • ms57
  • ms58
  • ms59
  • ms60
  • ms61
  • ms62
  • ms63
  • ms64
  • ms65
  • ms66
  • ms67
  • ms68
  • ms69
  • ms70
  • ms71
  • ms72
  • ms73
  • ms74
  • mt-IF-3
  • uL10m
  • uL11m
  • uL15m
  • uL22m
  • uL23m
  • uL24m
  • uL29m
  • uL30m
  • uL3m
  • uL4m
  • uS10m
  • uS11m
  • uS12
  • uS14m
  • uS15m
  • uS17m
  • uS19m
  • uS3m
  • uS55m
  • uS8m
  • uS9m
KeywordsRIBOSOME / mitoribosome / translation / Trypanosoma / large ribosomal subunit / small ribosomal subunit / 12S rRNA / 9S rRNA / ribosomal protein
Function / homology
Function and homology information


: / modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / organellar large ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast ...: / modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / organellar large ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast / quorum sensing / enoyl-CoA hydratase activity / regulation of protein kinase A signaling / mRNA stabilization / nuclear lumen / mitochondrial large ribosomal subunit / ciliary plasm / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / cyclosporin A binding / post-transcriptional regulation of gene expression / fatty acid beta-oxidation / superoxide dismutase / protein kinase A regulatory subunit binding / superoxide dismutase activity / ciliary basal body / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / large ribosomal subunit rRNA binding / large ribosomal subunit / protein folding / negative regulation of translation / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein S12 / Ribosomal protein S12 / Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / : / Probable Zinc-ribbon domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type ...Ribosomal protein S12 / Ribosomal protein S12 / Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / : / Probable Zinc-ribbon domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Cyclic phosphodiesterase / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / LIM domain / Pentatricopeptide repeat / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / LIM domain profile. / 39S ribosomal protein L46, mitochondrial / Rhodanese-like domain / DnaJ domain / LysM domain / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Rhodanese domain profile. / Phosphatidylethanolamine-binding protein, eukaryotic / Rhodanese-like domain superfamily / LysM domain / PEBP-like superfamily / Rhodanese-like domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / ANL, N-terminal domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / AMP-dependent synthetase/ligase / AMP-binding enzyme / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / ClpP/crotonase-like domain superfamily / NUDIX hydrolase-like domain superfamily / Zinc finger C2H2 superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein S18 superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ubiquitin family / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S8
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SERINE / SPERMIDINE / SPERMINE / URIDINE 5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) ...GUANOSINE-5'-TRIPHOSPHATE / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SERINE / SPERMIDINE / SPERMINE / URIDINE 5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Ribosomal protein S12 / Large ribosomal subunit protein uL4m / Replication restart DNA helicase PriA / Superoxide dismutase, putative / 39S ribosomal protein L55, mitochondrial / Uncharacterized protein / KOW domain-containing protein / Ubiquitin-like domain-containing protein / 39S ribosomal protein L32, mitochondrial / LETM1 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Chaperone protein DNAj, putative / TPR_REGION domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL22c / Uncharacterized protein / Large ribosomal subunit protein bL21m / MRP-L46 domain-containing protein / T. brucei spp.-specific protein / Uncharacterized protein / Ribosomal protein L30 ferredoxin-like fold domain-containing protein / Uncharacterized protein / T. brucei spp.-specific protein / Uncharacterized protein / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / Maxicircle unassigned reading frame 5 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S24/S35 mitochondrial conserved domain-containing protein / Large ribosomal subunit protein bL20c / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Large ribosomal subunit protein uL23m / Probable Zinc-ribbon domain-containing protein / Zn_Tnp_IS91 domain-containing protein / Uncharacterized protein / 30S ribosomal protein S8, putative / Uncharacterized protein / Small ribosomal subunit protein mS41 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Small ribosomal subunit protein uS5 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding complex 1 subunit / Trans-sialidase / Uncharacterized protein / 30S Ribosomal protein S17, putative / Uncharacterized protein / Large ribosomal subunit protein uL29m / Rhodanese domain-containing protein / Uncharacterized protein / Opioid growth factor receptor (OGFr) conserved domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L9 domain-containing protein / Uncharacterized protein / Uncharacterized protein / 50S ribosomal protein L16, putative / Uncharacterized protein / Ribosomal protein S23 mitochondrial conserved domain-containing protein
Similarity search - Component
Biological speciesTrypanosoma brucei brucei (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7.8 Å
AuthorsRamrath, D.J.F. / Niemann, M. / Leibundgut, M. / Bieri, P. / Prange, C. / Horn, E.K. / Leitner, A. / Boerhringer, D. / Schneider, A. / Ban, N.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation Switzerland
CitationJournal: Science / Year: 2018
Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
History
DepositionAug 31, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 3, 2018Provider: repository / Type: Initial release
Revision 1.1Oct 24, 2018Group: Advisory / Data collection / Derived calculations
Category: pdbx_validate_close_contact / struct_conn / struct_conn_type
Revision 1.2Nov 7, 2018Group: Data collection / Database references / Category: citation / Item: _citation.journal_volume

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Structure visualization

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  • Deposited structure unit
  • Imaged by Jmol
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  • Superimposition on EM map
  • EMDB-0229
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Structure viewerMolecule:
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Assembly

Deposited unit
DA: ms48
DD: ms51
DI: ms56
DL: ms59
DM: ms60
DN: ms61
DO: ms62
DP: ms63
DQ: ms64
DR: ms65
DS: ms66
DU: ms68
DZ: ms73
Da: ms74
DB: ms49
DC: ms50
DE: mS52
DF: ms53
DG: ms54
DH: ms55
DJ: ms57
DK: ms58
DT: ms67
DV: ms69
DW: ms70
DX: ms71
DY: ms72
CC: uS3m
CE: uS55m
CF: bS6m
CH: uS8m
CI: uS9m
CJ: uS10m
CK: uS11m
CL: uS12
CN: uS14m
CO: uS15m
CP: bS16m
CQ: uS17m
CR: bS18m
CS: uS19m
CU: bS12m
CZ: mt-IF-3
Ca: mS22
Cb: mS23
Cd: mS26
Cg: mS29
Ci: mS33
Cj: mS34
Ck: mS35
Cm: mS37
Cn: mS38
Cp: ms34
Cq: ms42
Cr: mS43
Cv: mS47
CA: 9s rRNA
UO: UNK
UP: UNK
UQ: UNK
UR: UNK
US: UNK
UT: UNK
A0: bL27m
A1: bL28m
A2: uL29m
A3: uL30m
A4: bL31m
A5: bL32m
A6: bL33m
A8: bL35m
A9: bL36m
AE: uL3m
AF: uL4m
AI: bL9m
AJ: uL10m
AK: uL11m
AN: 50S ribosomal protein L13, putative
AP: uL15m
AQ: 50S ribosomal protein L16, putative
AR: 50S ribosomal protein L17, putative
AT: bL19m
AU: bL20m
AV: bL21m
AW: uL22m
AX: uL23m
AY: uL24m
Ab: mL38
Ad: mL40
Ae: mL41
Af: mL42
Ag: mL43
Aj: mL46
Al: mL49
Ao: mL52
Ap: mL53
At: mL63
Av: mL68
AB: bL12m
AC: bL12m
AD: bL12m
AG: bL12m
AA: 12S rRNA
BA: mL67
BB: mL68
BC: mL69
BD: mL70
BE: mL71
BF: mL72
BG: mL73
BH: mL74
BI: mL75
BJ: mL76
BK: mL77
BL: mL78
BM: mL79
BN: mL80
BO: mL81
BP: mL82
BQ: mL83
BR: mL84
BS: mL85
BT: mL86
BU: mL87
BV: mL88
BW: mL89
BX: mL90
BY: mS91
BZ: mL92
Ba: mL93
Bb: mL94
Bc: mL95
Bd: mL96
Be: mL97
Bf: mL98
Bg: mL99
Bh: mL100
UA: UNK
UB: UNK
UC: UNK
UD: UNK
UE: UNK
UF: UNK
UG: UNK
UH: UNK
UI: UNK
UK: UNK
UL: UNK
UM: UNK
UN: UNK
UU: UNK
UV: UNK
UW: UNK
UX: UNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)5,396,177219
Polymers5,391,846154
Non-polymers4,33165
Water543
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 125 types, 125 molecules DADDDIDLDMDNDODPDQDRDSDUDZDaDBDCDEDFDGDHDJDKDTDVDWDXDYCCCECF...

#1: Protein ms48


Mass: 201811.188 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 1470-1487 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.5200 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UJ2
#2: Protein ms51


Mass: 95080.992 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.02.3180 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q385L8
#3: Protein ms56


Mass: 47306.836 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4560 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q587C2
#4: Protein ms59


Mass: 35395.676 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.4220 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38BS2
#5: Protein ms60


Mass: 34546.641 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII2960 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZSF8
#6: Protein ms61


Mass: 33482.340 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.211.4510 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38D60
#7: Protein ms62


Mass: 32068.100 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.3300 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383D1
#8: Protein ms63


Mass: 31922.178 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.160.3800 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38F25
#9: Protein ms64


Mass: 30742.207 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.5460 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q382E8
#10: Protein ms65


Mass: 30617.381 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_V5090 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZPP1
#11: Protein ms66


Mass: 29960.182 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.61.3110 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q388L7
#12: Protein ms68


Mass: 26472.807 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_III2230 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZKC1
#13: Protein ms73


Mass: 11360.885 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4580 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q587C4
#14: Protein ms74


Mass: 8203.408 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X9770 / Production host: Trypanosoma brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A3P2
#15: Protein ms49


Mass: 137234.125 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_II2470 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZJE4
#16: Protein ms50


Mass: 131719.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII3350 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZSK8
#17: Protein mS52


Mass: 84851.086 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q386Q7
#18: Protein ms53


Mass: 75338.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.160.4750 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38ET1
#19: Protein ms54


Mass: 72046.031 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.1790 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q57ZP8
#20: Protein ms55


Mass: 66676.680 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.5240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580V1
#21: Protein ms57


Mass: 45956.293 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.2080 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q584U8
#22: Protein ms58


Mass: 35623.910 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.3840 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38BP1
#23: Protein ms67


Mass: 30096.051 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4930 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586G5
#24: Protein ms69


Mass: 21589.732 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.4550 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q57UZ6
#25: Protein ms70


Mass: 21247.398 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_XI11660 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A8P6
#26: Protein ms71


Mass: 20093.227 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.2920 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383G5
#27: Protein ms72


Mass: 19317.293 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.3240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57YD4
#28: Protein uS3m


Mass: 9070.940 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Mitochondrial gene MURF-5 / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: MURF5 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: E0A3K1
#29: Protein uS55m


Mass: 50147.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.0530 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q38AX6
#30: Protein bS6m


Mass: 18861.543 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 105-114 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.4850 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38BW5
#31: Protein uS8m


Mass: 32766.459 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.389.0130 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q388R7
#32: Protein uS9m


Mass: 50201.016 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.3110 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q57W62
#33: Protein uS10m


Mass: 94351.039 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.4040 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57Z45
#34: Protein uS11m


Mass: 37925.910 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.406.0510 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q389T7
#35: Protein uS12


Mass: 10421.635 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Mitochondrial gene subject to RNA editing alternative start codon
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: RPS12 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: A0A0E3J9R6
#36: Protein uS14m


Mass: 19900.014 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.1430 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580I0
#37: Protein uS15m


Mass: 50401.191 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: TB927.1.1200 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q4GZ99
#38: Protein bS16m


Mass: 22077.805 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.02.5670 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q384N9
#39: Protein uS17m


Mass: 35632.727 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.211.2580 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38DP8
#40: Protein bS18m


Mass: 36988.691 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.6k15.3900 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38AS2
#41: Protein uS19m


Mass: 28364.604 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.2180 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q584T8
#42: Protein bS12m


Mass: 23087.889 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.4130 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580M9
#43: Protein mt-IF-3


Mass: 41086.285 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 294-304 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII1040 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister / References: UniProt: C9ZRZ4
#44: Protein mS22


Mass: 73064.141 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 20-37 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.211.2430 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q38DR3
#45: Protein mS23


Mass: 36784.500 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.1510 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57VB2
#46: Protein mS26


Mass: 51136.684 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 221-248 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.211.3000 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38DK6
#47: Protein mS29


Mass: 57048.836 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.1250 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585C2
#48: Protein mS33


Mass: 21244.104 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.770 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WW0
#49: Protein mS34


Mass: 28728.180 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.5280 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UK0
#50: Protein mS35


Mass: 98377.773 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.47.0024 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q387C7
#51: Protein mS37


Mass: 24757.896 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.6600 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38C96
#52: Protein mS38


Mass: 28240.600 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.630 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57VQ9
#53: Protein ms34


Mass: 22421.469 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.26.0750 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q389L3
#54: Protein ms42


Mass: 29748.666 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: TEOVI_000558800 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: A0A1G4I0W4, superoxide dismutase
#55: Protein mS43


Mass: 48590.410 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 27-35 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.3690 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585I1
#56: Protein mS47


Mass: 137935.000 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.1910 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383R4
#62: Protein UNK


Mass: 4613.678 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#64: Protein bL27m


Mass: 21793.092 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: TEOVI_000532300 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: A0A1G4I8T3
#65: Protein bL28m


Mass: 28051.373 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4040 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586A2
#66: Protein uL29m


Mass: 54045.367 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.160.5240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38EM7
#67: Protein uL30m


Mass: 25204.334 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_IX4520 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZY77
#68: Protein bL31m


Mass: 21083.229 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.6k15.1920 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38AB0
#69: Protein bL32m


Mass: 9745.710 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.2330 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q584F4
#70: Protein bL33m


Mass: 13042.740 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.1810 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q581Q0
#71: Protein bL35m


Mass: 21661.453 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X2260 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A1K1
#72: Protein bL36m


Mass: 21795.227 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_IV4820 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZMA9
#73: Protein uL3m


Mass: 55073.699 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.5610 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580R4
#74: Protein uL4m


Mass: 53156.664 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: TEOVI_000582800 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: A0A1G4HYD1
#75: Protein bL9m


Mass: 31032.697 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.3410 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UC5
#76: Protein uL10m


Mass: 20283.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 106-129 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_V3970 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZPD1
#77: Protein uL11m


Mass: 40252.750 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.2.4740, Tb927.2.4890 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586R9
#79: Protein uL15m


Mass: 43780.016 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.3980 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57U68
#82: Protein bL19m


Mass: 16931.455 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: TB927.1.1210 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q4GZ98
#83: Protein bL20m


Mass: 24393.943 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.1930 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383R2
#84: Protein bL21m


Mass: 20895.268 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII4650 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZT11
#85: Protein uL22m


Mass: 32083.285 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII3160 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZSI8
#86: Protein uL23m


Mass: 28822.936 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.03.0260 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q387G3
#87: Protein uL24m


Mass: 43999.047 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 313-340 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_III1550 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZK52
#88: Protein mL38


Mass: 58437.895 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 41-53 and 294-311 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.6620 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381T7
#89: Protein mL40


Mass: 33646.066 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X14530 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A511
#90: Protein mL41


Mass: 22556.877 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_XI11060 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A8I6
#91: Protein mL42


Mass: 21602.639 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.1840 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383S1
#92: Protein mL43


Mass: 31300.406 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.4600 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q583E5
#93: Protein mL46


Mass: 33984.199 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 149-159 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII5370 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZT91
#94: Protein mL49


Mass: 24501.486 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 102-109 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.5.3110 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57Z82
#95: Protein mL52


Mass: 29175.984 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.02.2250 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q385V2
#96: Protein mL53


Mass: 35656.383 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.2990, Tb927.7.3030 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57YA9
#97: Protein mL63


Mass: 18213.924 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 64-73 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VII8230 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZU82
#98: Protein mL68


Mass: 28545.977 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.3500 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383B7
#99: Protein bL12m


Mass: 3998.370 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote)
#103: Protein mL67


Mass: 93569.273 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_XI1730 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A5V6
#104: Protein mL68


Mass: 60896.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 264-343 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.7650 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38CI0
#105: Protein mL69


Mass: 59010.855 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.2010 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q584V5
#106: Protein mL70


Mass: 62442.410 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: L1-stalk protein residues built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VI3990 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZR91
#107: Protein mL71


Mass: 50313.797 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.3460 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WG1
#108: Protein mL72


Mass: 49407.000 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VI3710 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZR63
#109: Protein mL73


Mass: 41857.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.6800 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q57Y49
#110: Protein mL74


Mass: 39765.176 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.6k15.3290 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38AM5
#111: Protein mL75


Mass: 39841.617 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.70.7960 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q38CK0
#112: Protein mL76


Mass: 39638.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 168-176 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.2340 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383M2
#113: Protein mL77


Mass: 42731.605 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 248-281 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VI2240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZQR6
#114: Protein mL78


Mass: 35104.535 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X13360 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A4P5
#115: Protein mL79


Mass: 33079.617 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.7140 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381N5
#116: Protein mL80


Mass: 34252.094 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 145-174 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.1440 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585A3
#117: Protein mL81


Mass: 29284.467 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_XI6240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A755
#118: Protein mL82


Mass: 29283.676 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 153-162 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X19420 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A0S6
#119: Protein mL83 / PPIase


Mass: 25579.004 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.3430 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WF8, peptidylprolyl isomerase
#120: Protein mL84


Mass: 24773.643 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.4080 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q586A6
#121: Protein mL85


Mass: 18817.680 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.160.2250 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38FG8
#122: Protein mL86


Mass: 22376.191 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_V5660 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZPU8
#123: Protein mL87


Mass: 22337.521 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_XI9190 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A7Z9
#124: Protein mL88


Mass: 22255.590 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_VIII1510 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZUW3
#125: Protein mL89


Mass: 22213.406 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.820 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WW5
#126: Protein mL90


Mass: 22123.414 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.6.1700 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585P1
#127: Protein mS91


Mass: 20618.520 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.3.5300 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580U5
#128: Protein mL92 / PPIase


Mass: 20542.291 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 46-64 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.1215 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q383Y4, peptidylprolyl isomerase
#129: Protein mL93


Mass: 17343.738 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972 / Gene: TbgDal_X13710 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A4T0
#130: Protein mL94


Mass: 17830.959 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb09.160.5050 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38EP7
#131: Protein mL95


Mass: 17803.377 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.26.0585 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q389K3
#132: Protein mL96


Mass: 16387.654 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb11.01.6285 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381W9
#133: Protein mL97


Mass: 12974.785 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.61.3115 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q388L8
#134: Protein mL98


Mass: 12968.644 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb10.61.3155 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q388M2
#135: Protein mL99


Mass: 12334.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.2.2590 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q587H8
#136: Protein mL100


Mass: 10600.362 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Gene: TEOVI_000790200 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: A0A1G4HYZ0
#140: Protein UNK


Mass: 15081.565 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427

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RNA chain , 2 types, 2 molecules CAAA

#57: RNA chain 9s rRNA


Mass: 198442.391 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: GenBank: 343546
#102: RNA chain 12S rRNA / MT-RNR1


Mass: 377015.625 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: GenBank: 343546

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Protein/peptide , 17 types, 24 molecules UOUPUWUQURUVUXUTACADAGUAUBUCUHUEUFUGUNUIUKULUMUU

#58: Protein/peptide UNK


Mass: 443.539 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote)
#59: Protein/peptide UNK


Mass: 613.749 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#60: Protein/peptide UNK


Mass: 2741.370 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#61: Protein/peptide UNK


Mass: 698.854 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#63: Protein/peptide UNK


Mass: 3762.629 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#100: Protein/peptide bL12m


Mass: 2008.194 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#101: Protein/peptide bL12m


Mass: 1937.116 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote)
#137: Protein/peptide UNK


Mass: 3932.839 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#138: Protein/peptide UNK


Mass: 3422.209 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote)
#139: Protein/peptide UNK


Mass: 1039.273 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#141: Protein/peptide UNK


Mass: 1890.321 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote)
#142: Protein/peptide UNK


Mass: 2060.531 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#143: Protein/peptide UNK


Mass: 1464.797 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#144: Protein/peptide UNK


Mass: 869.063 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#145: Protein/peptide UNK


Mass: 1294.587 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#146: Protein/peptide UNK


Mass: 528.644 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#147: Protein/peptide UNK


Mass: 954.168 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427

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50S ribosomal protein ... , 3 types, 3 molecules ANAQAR

#78: Protein 50S ribosomal protein L13, putative / Ribosome


Mass: 24341.309 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.1070 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580D5
#80: Protein 50S ribosomal protein L16, putative / Ribosome


Mass: 19158.145 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.7.3960 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UM6
#81: Protein 50S ribosomal protein L17, putative / Ribosome


Mass: 35117.824 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.8.5860 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57YI7

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Non-polymers , 9 types, 68 molecules

#148: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: Zn
#149: Chemical
ChemComp-SPD / SPERMIDINE / N-(2-AMINO-PROPYL)-1,4-DIAMINOBUTANE / PA(34) / Spermidine


Mass: 145.246 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C7H19N3
#150: Chemical ChemComp-UTP / URIDINE 5'-TRIPHOSPHATE / Uridine triphosphate


Mass: 484.141 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C9H15N2O15P3 / Comment: UTP*YM
#151: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 46 / Source method: obtained synthetically / Formula: Mg
#152: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate


Mass: 523.180 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#153: Chemical ChemComp-SPM / SPERMINE / Spermine


Mass: 202.340 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H26N4
#154: Chemical ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / Nicotinamide adenine dinucleotide


Mass: 663.425 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C21H27N7O14P2 / Comment: NAD*YM
#155: Chemical ChemComp-SER / SERINE / Serine


Type: L-peptide linking / Mass: 105.093 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H7NO3
#156: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: the complete T. brucei mitoribosome with its small and large subunits
Type: RIBOSOME / Entity ID: #1-#147 / Source: NATURAL
Molecular weightValue: 4.5 MDa / Experimental value: YES
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE-PROPANE / Humidity: 98 % / Chamber temperature: 278 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)

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Processing

EM software
IDNameCategory
1EMANparticle selection
4CTFFINDCTF correction
7UCSF Chimeramodel fitting
11RELIONclassification
12RELION3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 7.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 7141 / Algorithm: FOURIER SPACE / Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT

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Related info.:EMDB header

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Aug 12, 2020. Covid-19 info

Covid-19 info

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New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

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Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

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