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- EMDB-0229: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -

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Basic information

Entry
Database: EMDB / ID: 0229
TitleCryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete mitoribosome
Map datamap of the complete T. brucei mitoribosome
Samplethe complete T. brucei mitoribosome with its small and large subunits:
ms48 / ms51 / ms56 / ms59 / ms60 / ms61 / ms62 / ms63 / ms64 / ms65 ...ms48 / ms51 / ms56 / ms59 / ms60 / ms61 / ms62 / ms63 / ms64 / ms65 / ms66 / ms68 / ms73 / ms74 / ms49 / ms50 / mS52 / ms53 / ms54 / ms55 / ms57 / ms58 / ms67 / ms69 / ms70 / ms71 / ms72 / uS3m / uS55m / bS6m / uS8m / uS9m / uS10m / uS11m / uS12 / uS14m / uS15m / bS16m / uS17m / bS18m / uS19m / bS12m / mt-IF-3 / mS22 / mS23 / mS26 / mS29 / mS33 / mS34 / mS35 / mS37 / mS38 / ms34 / ms42 / mS43 / mS47 / (nucleic-acidNucleic acid) x 2 / (UNK) x 17 / bL27m / bL28m / uL29m / uL30m / bL31m / bL32m / bL33m / bL35m / bL36m / uL3m / uL4m / bL9m / uL10m / uL11m / (50S ribosomal protein ...) x 3 / uL15m / bL19m / bL20m / bL21m / uL22m / uL23m / uL24m / mL38 / mL40 / mL41 / mL42 / mL43 / mL46 / mL49 / mL52 / mL53 / mL63 / (mL68) x 2 / (bL12m) x 3 / mL67 / mL69 / mL70 / mL71 / mL72 / mL73 / mL74 / mL75 / mL76 / mL77 / mL78 / mL79 / mL80 / mL81 / mL82 / mL83 / mL84 / mL85 / mL86 / mL87 / mL88 / mL89 / mL90 / mS91 / mL92 / mL93 / mL94 / mL95 / mL96 / mL97 / mL98 / mL99 / mL100 / (ligand) x 9
Function / homologyRibosomal protein S9/S16 / Ribosomal protein L21-like / Ribosomal protein L46, N-terminal / Ribosomal protein L4 domain superfamily / Ribosomal protein S16 domain superfamily / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / Probable Zinc-ribbon domain / Ribosomal protein L28/L24 / Cyclophilin-like domain superfamily / Ribosomal protein L11, N-terminal ...Ribosomal protein S9/S16 / Ribosomal protein L21-like / Ribosomal protein L46, N-terminal / Ribosomal protein L4 domain superfamily / Ribosomal protein S16 domain superfamily / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / Probable Zinc-ribbon domain / Ribosomal protein L28/L24 / Cyclophilin-like domain superfamily / Ribosomal protein L11, N-terminal / ClpP/crotonase-like domain superfamily / Alpha/Beta hydrolase fold / Ubiquitin-like domain superfamily / Mitochondrial 28S ribosomal protein S34 / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Ribosomal protein S12 / Ribosomal protein L20, C-terminal / Ribosomal protein L18e/L15P / Ribosomal protein L11, C-terminal / Ribosomal protein S8 superfamily / Ribosomal protein L10e/L16 / 50S ribosomal protein uL4 / Ribosomal protein L25/L23 / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein L2, domain 2 / NUDIX hydrolase-like domain superfamily / Ribosomal protein L30, ferredoxin-like fold domain / LysM domain / Ribosomal protein S5 domain 2-type fold / IGR protein motif / Ribosomal protein S23/S29, mitochondrial / Protein kinase A anchor protein, nuclear localisation signal domain / Manganese/iron superoxide dismutase, N-terminal / Manganese/iron superoxide dismutase, C-terminal / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L9, N-terminal / Phosphatidylethanolamine-binding protein, eukaryotic / L21-like superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L23 / Ribosomal protein L33 superfamily / Iron/manganese superoxide dismutases, alpha-hairpin domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / DnaJ domain / Ribosomal protein L22p/L17e / Ubiquitin family / Ribosomal protein L16p/L10e / Ribosomal protein L3 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein S15 / Ribosomal protein L30p/L7e / Ribosomal protein S17 / Ribosomal protein L4/L1 family / Ribosomal protein S8 / LIM domain / MRP-L47 superfamily, mitochondrial / Ribosomal protein S11 superfamily / Ribosomal L18e/L15P superfamily / Ribosomal protein L11, C-terminal domain superfamily / Zinc finger C2H2 superfamily / Thioredoxin-like superfamily / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Ribosomal protein L17 superfamily / Ribosomal protein L22/L17 superfamily / PEBP-like superfamily / LysM domain superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Chaperone J-domain superfamily / Ribosomal protein S18 superfamily / Rhodanese-like domain superfamily / Nucleoid-associated protein YbaB-like domain superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L23/L15e core domain superfamily / Nucleic acid-binding, OB-fold / Ribosomal protein L13 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Ribosomal protein L9 / Ribosomal protein L16 / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Tetratricopeptide-like helical domain superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L17 / Ribosomal protein L9, N-terminal domain / LysM domain / Iron/manganese superoxide dismutases, C-terminal domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein L11, N-terminal domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal death-associated protein 3 / Ribosomal protein L17 / AKAP7 2'5' RNA ligase-like domain / 39S mitochondrial ribosomal protein L46 / Probable Zinc-ribbon domain / Mitochondrial 28S ribosomal protein S34 / Enoyl-CoA hydratase/isomerase
Function and homology information
SourceTrypanosoma brucei brucei (eukaryote)
Methodsingle particle reconstruction / cryo EM / 7.8 Å resolution
AuthorsRamrath DJF / Niemann M / Leibundgut M / Bieri P / Prange C / Horn EK / Leitner A / Boerhringer D / Schneider A / Ban N
CitationJournal: Science / Year: 2018
Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban
Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
Validation ReportPDB-ID: 6hiv

SummaryFull reportAbout validation report
DateDeposition: Aug 31, 2018 / Header (metadata) release: Oct 3, 2018 / Map release: Oct 3, 2018 / Last update: Nov 7, 2018

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.054
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.054
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-6hiv
  • Surface level: 0.054
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_0229.map.gz (map file in CCP4 format, 256001 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
400 pix
1.39 Å/pix.
= 556. Å
400 pix
1.39 Å/pix.
= 556. Å
400 pix
1.39 Å/pix.
= 556. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.39 Å
Density
Contour Level:0.054 (by author), 0.054 (movie #1):
Minimum - Maximum-0.089613646 - 0.18272288
Average (Standard dev.)0.0019386684 (0.011937334)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions400400400
Origin0.00.00.0
Limit399.0399.0399.0
Spacing400400400
CellA=B=C: 556.0 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z556.000556.000556.000
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0900.1830.002

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Supplemental data

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Sample components

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Entire the complete T. brucei mitoribosome with its small and large subunits

EntireName: the complete T. brucei mitoribosome with its small and large subunits
Number of components: 157

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Component #1: protein, the complete T. brucei mitoribosome with its small and l...

ProteinName: the complete T. brucei mitoribosome with its small and large subunits
Recombinant expression: No
MassExperimental: 4.5 MDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427

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Component #2: protein, ms48

ProteinName: ms48 / Details: residues 1470-1487 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 201.811188 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #3: protein, ms51

ProteinName: ms51 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 95.080992 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #4: protein, ms56

ProteinName: ms56 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 47.306836 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #5: protein, ms59

ProteinName: ms59 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.395676 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #6: protein, ms60

ProteinName: ms60 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.546641 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #7: protein, ms61

ProteinName: ms61 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.48234 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #8: protein, ms62

ProteinName: ms62 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.0681 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #9: protein, ms63

ProteinName: ms63 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.922178 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #10: protein, ms64

ProteinName: ms64 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.742207 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #11: protein, ms65

ProteinName: ms65 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.617381 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #12: protein, ms66

ProteinName: ms66 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.960182 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #13: protein, ms68

ProteinName: ms68 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.472807 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #14: protein, ms73

ProteinName: ms73 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.360885 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #15: protein, ms74

ProteinName: ms74 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.203408 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei (eukaryote)

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Component #16: protein, ms49

ProteinName: ms49 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 137.234125 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #17: protein, ms50

ProteinName: ms50 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 131.71925 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #18: protein, mS52

ProteinName: mS52 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 84.851086 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #19: protein, ms53

ProteinName: ms53 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 75.338914 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #20: protein, ms54

ProteinName: ms54 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 72.046031 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #21: protein, ms55

ProteinName: ms55 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 66.67668 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #22: protein, ms57

ProteinName: ms57 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.956293 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #23: protein, ms58

ProteinName: ms58 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.62391 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #24: protein, ms67

ProteinName: ms67 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 30.096051 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #25: protein, ms69

ProteinName: ms69 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.589732 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #26: protein, ms70

ProteinName: ms70 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.247398 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #27: protein, ms71

ProteinName: ms71 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.093227 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #28: protein, ms72

ProteinName: ms72 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.317293 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #29: protein, uS3m

ProteinName: uS3m / Details: Mitochondrial gene MURF-5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.07094 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #30: protein, uS55m

ProteinName: uS55m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 50.147355 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #31: protein, bS6m

ProteinName: bS6m / Details: residues 105-114 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.861543 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #32: protein, uS8m

ProteinName: uS8m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.766459 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #33: protein, uS9m

ProteinName: uS9m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 50.201016 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #34: protein, uS10m

ProteinName: uS10m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 94.351039 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #35: protein, uS11m

ProteinName: uS11m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 37.92591 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #36: protein, uS12

ProteinName: uS12
Details: Mitochondrial gene subject to RNA editing alternative start codon
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.421635 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #37: protein, uS14m

ProteinName: uS14m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.900014 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #38: protein, uS15m

ProteinName: uS15m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 50.401191 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #39: protein, bS16m

ProteinName: bS16m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.077805 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #40: protein, uS17m

ProteinName: uS17m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.632727 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #41: protein, bS18m

ProteinName: bS18m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 36.988691 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #42: protein, uS19m

ProteinName: uS19m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.364604 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #43: protein, bS12m

ProteinName: bS12m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.087889 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #44: protein, mt-IF-3

ProteinName: mt-IF-3 / Details: residues 294-304 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 41.086285 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #45: protein, mS22

ProteinName: mS22 / Details: residues 20-37 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 73.064141 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #46: protein, mS23

ProteinName: mS23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 36.7845 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #47: protein, mS26

ProteinName: mS26 / Details: residues 221-248 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 51.136684 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #48: protein, mS29

ProteinName: mS29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 57.048836 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

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Component #49: protein, mS33

ProteinName: mS33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.244104 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #50: protein, mS34

ProteinName: mS34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.72818 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #51: protein, mS35

ProteinName: mS35 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 98.377773 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #52: protein, mS37

ProteinName: mS37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.757896 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #53: protein, mS38

ProteinName: mS38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.2406 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #54: protein, ms34

ProteinName: ms34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.421469 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #55: protein, ms42

ProteinName: ms42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.748666 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #56: protein, mS43

ProteinName: mS43 / Details: residues 27-35 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 48.59041 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #57: protein, mS47

ProteinName: mS47 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 137.935 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #58: nucleic-acid, 9s rRNA

Nucleic-acidName: 9s rRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAUA AAAUUUUAGG AAUAGUUAAU AAUAAUUUAU AAUUUUGAUU AGAUUGUUUU GUUAAUGCUA UUAGAUGGGU GUGGAAAAAU AAAAAAAAUA AUUAAUAUAU AUCAAUAAUA AAUUAAAUUA AUCUAUUAGU CAGAAAUGGA UGCCAGCCGU UGCGGUAAUU UCUAUGCUUU UAAAUAUUAU ACAAUUAUCA UAUUAAAUUG UUAAGUGUUG AUUUAACCAA UAAAAAUAUA AAUAAUUUUU AUUUGUUUUU AAACACCAUU AGGUAUAUGC AAAUAUAAAA UUAUAGUAAU UAUAAAUUAU AUUAUAUUAU AUUUAUUCAU AUAAUUAAUA GGAUAAUAUU UGUAGUUUUU GAUACCAUGA UAAGGAUUAU AAAUUGAAAG UGUUAAUAUC AUAAUCAAAA UUUAUUAUUU AUAUUAAAUA UGUAUGUGUA GAUAAAAUAA GAAAUUAAAA AGGUAUUGUU GCCCACCAAU UUUUAUAAUA AAAAUAACGU GCAGUAAUUA AUAUAUUUAU AAAAAUAUAU UUUUUUUUUU U
MassTheoretical: 198.442391 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)

+
Component #59: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.443539 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #60: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 0.613749 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #61: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.74137 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #62: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 0.698854 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #63: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 4.613678 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #64: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.762629 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #65: protein, bL27m

ProteinName: bL27m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.793092 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #66: protein, bL28m

ProteinName: bL28m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.051373 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #67: protein, uL29m

ProteinName: uL29m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 54.045367 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #68: protein, uL30m

ProteinName: uL30m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.204334 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #69: protein, bL31m

ProteinName: bL31m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.083229 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #70: protein, bL32m

ProteinName: bL32m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.74571 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #71: protein, bL33m

ProteinName: bL33m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.04274 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #72: protein, bL35m

ProteinName: bL35m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.661453 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #73: protein, bL36m

ProteinName: bL36m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.795227 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #74: protein, uL3m

ProteinName: uL3m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 55.073699 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #75: protein, uL4m

ProteinName: uL4m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 53.156664 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #76: protein, bL9m

ProteinName: bL9m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.032697 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #77: protein, uL10m

ProteinName: uL10m / Details: residues 106-129 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.283566 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #78: protein, uL11m

ProteinName: uL11m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 40.25275 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #79: protein, 50S ribosomal protein L13, putative

ProteinName: 50S ribosomal protein L13, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.341309 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #80: protein, uL15m

ProteinName: uL15m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.780016 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #81: protein, 50S ribosomal protein L16, putative

ProteinName: 50S ribosomal protein L16, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.158145 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #82: protein, 50S ribosomal protein L17, putative

ProteinName: 50S ribosomal protein L17, putativeRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.117824 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #83: protein, bL19m

ProteinName: bL19m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.931455 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #84: protein, bL20m

ProteinName: bL20m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.393943 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #85: protein, bL21m

ProteinName: bL21m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.895268 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #86: protein, uL22m

ProteinName: uL22m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 32.083285 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #87: protein, uL23m

ProteinName: uL23m / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.822936 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #88: protein, uL24m

ProteinName: uL24m / Details: residues 313-340 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.999047 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #89: protein, mL38

ProteinName: mL38 / Details: residues 41-53 and 294-311 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 58.437895 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #90: protein, mL40

ProteinName: mL40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.646066 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #91: protein, mL41

ProteinName: mL41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.556877 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #92: protein, mL42

ProteinName: mL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.602639 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #93: protein, mL43

ProteinName: mL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.300406 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #94: protein, mL46

ProteinName: mL46 / Details: residues 149-159 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.984199 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #95: protein, mL49

ProteinName: mL49 / Details: residues 102-109 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.501486 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #96: protein, mL52

ProteinName: mL52 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.175984 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #97: protein, mL53

ProteinName: mL53 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.656383 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #98: protein, mL63

ProteinName: mL63 / Details: residues 64-73 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.213924 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #99: protein, mL68

ProteinName: mL68 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.545977 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #100: protein, bL12m

ProteinName: bL12m / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.99837 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #101: protein, bL12m

ProteinName: bL12m / Details: residues built as UNK / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 2.008194 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #102: protein, bL12m

ProteinName: bL12m / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.937116 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #103: nucleic-acid, 12S rRNA

Nucleic-acidName: 12S rRNAMT-RNR1 / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUUUUACCAA UUAAGAAGAA UAUUAUAAUA AUGGGUGUCU UAUAUUUUAA AUAAAUAUUU AAAUUCCGUG UAGUAAAUUU AUUAUUUGUA UUAUUUAUAU AAUAGGUGUA UUAUAUUUAA AUUUUAAAUU UGUUGUUUUA UAUUUAGAUA CAUAUUUAUA GAUUAAUAUA UUUAAAUAAU AUUUUAAAAU UUAUUGAACU GUAAUUAUUA GUUUAAUAUU UUUAGUUUGA UGUUGAAAUA UUUAAUUAAA GAUGUUACAG UUGUUCUAUA UGUACCAAAU AAAUAUAGUA AGAUUAUUUU AGUUGAAUUA AUAAAUAAAU AUUUAUUUUU CUUUGUAAAU AUUAUGAACA AUUUAAAAAU UAAUCUGUUU AACUAAAAUG UUAUAUAUAA UAAUCUAAGU UAAUUUGAAU AUUAAAAGUA CAAGUAUAAU UUGUAAUUCU AAAGUAUAUU AAUUUUAUAU UUUUAGUAGG UAAAUGAAAA GUAUAAAUGG AUAUAACUUA AUAUUUAAUA UUUGUUUAAU GAAAAGUAUU UUAUUAUUAU AUUGUAUAGU AUUAUUAUAG UGUAUAGUUU UUUAAAAAUA UAAAAAUAUU GUUAAUAAAA UUAUCGUAUU UUAAGUGCGU UAAUUAAAUG CGUUUAUCUA AGAUAAUUAU UUAAGAUUAU UCUUGUAAAU AUAUUUAAAU AUUAAUAAUU CUUAAAAUAA AGAAACAUCC UCAAUUGCAA UAUUAUUGUA GCAUAGUAAU UUCUUAACUA AGUAUUUAAU UUUUCCAUAG AAAAUUUUUA AAUUACAAGA AAGAAAAUAA AGUAUGAAUU AAUAUCAAAA UUUUAAUAAA AAUUAAAAAA UUAAAAUAGG GCAAGUCCUA CUCUCCUUUA CAAAGAGAAA CAUUAUGAUA UGUAAUUGUA UGUUUGAUUG GGGCAAUACU AUAUUUAUUU AUAUAGCAUA AGAACUAUAU UCUUUGAAAU UAUAAAAGGU UCGAGCAGGU UAACAAGCAU UAAAAAUAAA UGUGUUUCAU CGUCUACUUA UUACCAUGAU UGAUUGUUCA UCAAAAUAGU AAUUCGUUAG UUGGGUUAAA AUCGUUGUAA AGCAGAUUUG UUUAUAUAUU UAAUUUUUAU AAUUAAUAAU AAUUAAUAUA AGUACGCAAG GAUUGAUUAU UGAAAAAAGA AAGAAGAAUA UAAUUUAUA
MassTheoretical: 377.015625 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)

+
Component #104: protein, mL67

ProteinName: mL67 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 93.569273 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #105: protein, mL68

ProteinName: mL68 / Details: residues 264-343 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 60.896355 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #106: protein, mL69

ProteinName: mL69 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 59.010855 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #107: protein, mL70

ProteinName: mL70 / Details: L1-stalk protein residues built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 62.44241 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #108: protein, mL71

ProteinName: mL71 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 50.313797 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #109: protein, mL72

ProteinName: mL72 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 49.407 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #110: protein, mL73

ProteinName: mL73 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 41.857875 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #111: protein, mL74

ProteinName: mL74 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.765176 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #112: protein, mL75

ProteinName: mL75 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.841617 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #113: protein, mL76

ProteinName: mL76 / Details: residues 168-176 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.638914 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #114: protein, mL77

ProteinName: mL77 / Details: residues 248-281 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 42.731605 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #115: protein, mL78

ProteinName: mL78 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 35.104535 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #116: protein, mL79

ProteinName: mL79 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.079617 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #117: protein, mL80

ProteinName: mL80 / Details: residues 145-174 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.252094 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #118: protein, mL81

ProteinName: mL81 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.284467 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #119: protein, mL82

ProteinName: mL82 / Details: residues 153-162 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.283676 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #120: protein, mL83

ProteinName: mL83 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.579004 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #121: protein, mL84

ProteinName: mL84 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.773643 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #122: protein, mL85

ProteinName: mL85 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.81768 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #123: protein, mL86

ProteinName: mL86 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.376191 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #124: protein, mL87

ProteinName: mL87 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.337521 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #125: protein, mL88

ProteinName: mL88 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.25559 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #126: protein, mL89

ProteinName: mL89 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.213406 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #127: protein, mL90

ProteinName: mL90 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.123414 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #128: protein, mS91

ProteinName: mS91 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.61852 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #129: protein, mL92

ProteinName: mL92 / Details: residues 46-64 built as polyalanines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.542291 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #130: protein, mL93

ProteinName: mL93 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.343738 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: MHOM/CI/86/DAL972
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #131: protein, mL94

ProteinName: mL94 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.830959 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #132: protein, mL95

ProteinName: mL95 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.803377 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #133: protein, mL96

ProteinName: mL96 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.387654 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #134: protein, mL97

ProteinName: mL97 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.974785 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #135: protein, mL98

ProteinName: mL98 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.968644 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #136: protein, mL99

ProteinName: mL99 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.334969 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #137: protein, mL100

ProteinName: mL100 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.600362 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote)
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #138: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.932839 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #139: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.422209 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #140: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 1.039273 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #141: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.081565 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #142: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.890321 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #143: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 2.060531 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #144: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.464797 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #145: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.869063 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #146: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.294587 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #147: protein, UNK

ProteinName: UNK / Details: residues built as UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.528644 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #148: protein, UNK

ProteinName: UNK / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.954168 kDa
SourceSpecies: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Source (engineered)Expression System: Trypanosoma brucei brucei (eukaryote)

+
Component #149: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 10 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

+
Component #150: ligand, SPERMIDINE

LigandName: SPERMIDINE / Number of Copies: 4 / Recombinant expression: No
MassTheoretical: 0.145246 kDa

+
Component #151: ligand, URIDINE 5'-TRIPHOSPHATE

LigandName: URIDINE 5'-TRIPHOSPHATE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.484141 kDa

+
Component #152: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 46 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #153: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa

+
Component #154: ligand, SPERMINE

LigandName: SPERMINE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.20234 kDa

+
Component #155: ligand, NICOTINAMIDE-ADENINE-DINUCLEOTIDE

LigandName: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.663425 kDa

+
Component #156: ligand, SERINE

LigandName: SERINE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.105093 kDa

+
Component #157: ligand, water

LigandName: water / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 1.801505 MDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionpH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: OTHER / Temperature: 278 K / Humidity: 98 %

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 4 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON III (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 7141
3D reconstructionAlgorithm: FOURIER SPACE / Software: RELION / Resolution: 7.8 Å / Resolution method: FSC 0.143 CUT-OFF

-
Atomic model buiding

Modeling #1Refinement protocol: rigid body
Output model

+
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