EMDB-0230: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom...

Basic information

• Entry: Database: EMDB / ID: EMD-0230
• Title: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3
• Map data: map of the T. brucei mitoribosome small subunit in complex with mt-IF3

Sample

• Complex: small subunit of the T. brucei mitoribosome in complex with mt-IF-3
  • Protein or peptide: x 62 types
  • RNA: x 1 types
• Ligand: x 7 types

Function / homology

Function:

modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / thiosulfate sulfurtransferase activity / kinetoplast / quorum sensing / enoyl-CoA hydratase activity / regulation of protein kinase A signaling / mRNA stabilization / nuclear lumen / ciliary plasm / mitochondrial small ribosomal subunit / fatty acid beta-oxidation / superoxide dismutase / protein kinase A regulatory subunit binding / superoxide dismutase activity / ribosome / structural constituent of ribosome / translation / mitochondrion / RNA binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

Nucleoid-associated protein YbaB-like domain superfamily / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / : / Probable Zinc-ribbon domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Cyclic phosphodiesterase / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Rhodanese-like domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / ClpP/crotonase-like domain superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ubiquitin family / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S8 / Ribosomal protein S5, C-terminal domain / Ubiquitin-like domain / Ribosomal protein S9 / Ribosomal protein S17/S11 / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal protein S11 superfamily / Ubiquitin domain profile. / Tetratricopeptide-like helical domain superfamily / S15/NS1, RNA-binding / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold

Component:

A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Probable Zinc-ribbon domain-containing protein / 30S ribosomal protein S8, putative / Small ribosomal subunit protein mS41 / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Small ribosomal subunit protein uS5 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Trans-sialidase / Uncharacterized protein / 30S Ribosomal protein S17, putative / Uncharacterized protein / Uncharacterized protein / Opioid growth factor receptor (OGFr) conserved domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S23 mitochondrial conserved domain-containing protein / Uncharacterized protein / Ribosomal protein S9 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / LysM domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Ubiquitin-like domain-containing protein / Uncharacterized protein / Uncharacterized protein / Small ribosomal subunit protein mS29 / Manganese/iron superoxide dismutase C-terminal domain-containing protein / Superoxide dismutase, putative / Rhodanese domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein

• Biological species: Trypanosoma brucei brucei (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.37 Å
• Authors: Ramrath D / Niemann M / Leibundgut M / Bieri P / Prange C / Horn EK / Leitner A / Boehringer D / Schneider A / Ban N

Funding support

Switzerland, 1 items

Organization	Grant number	Country
Swiss National Science Foundation		Switzerland

• Citation: Journal: Science / Year: 2018; Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.; Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban / ; Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.

History

Deposition	Aug 31, 2018	-
Header (metadata) release	Sep 26, 2018	-
Map release	Sep 26, 2018	-
Update	Dec 18, 2019	-
Current status	Dec 18, 2019	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_0230.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: map of the T. brucei mitoribosome small subunit in complex with mt-IF3
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.0114 / Movie #1: 0.15
Minimum - Maximum	-0.31565467 - 0.53773916
Average (Standard dev.)	0.0025419195 (±0.02259299)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 320 320 320 Spacing 320 320 320
Cell	A=B=C: 444.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	320	320	320
origin x/y/z	0.000	0.000	0.000
length x/y/z	444.800	444.800	444.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	320	320	320
D min/max/mean	-0.316	0.538	0.003

Supplemental data

Half map: half map (odd) of the T. brucei mitoribosome...

• File: emd_0230_half_map_1.map
• Annotation: half map (odd) of the T. brucei mitoribosome small subunit in complex with mt-IF3

Half map: half map (even) of the T. brucei mitoribosome...

• File: emd_0230_half_map_2.map
• Annotation: half map (even) of the T. brucei mitoribosome small subunit in complex with mt-IF3

Sample components

Entire : small subunit of the T. brucei mitoribosome in complex with mt-IF-3

• Entire: Name: small subunit of the T. brucei mitoribosome in complex with mt-IF-3

Components

• Complex: small subunit of the T. brucei mitoribosome in complex with mt-IF-3
  • Protein or peptide: mS48
  • Protein or peptide: mS51
  • Protein or peptide: mS56
  • Protein or peptide: mS59
  • Protein or peptide: mS60
  • Protein or peptide: mS61
  • Protein or peptide: mS62
  • Protein or peptide: mS63
  • Protein or peptide: mS64
  • Protein or peptide: mS65
  • Protein or peptide: mS66
  • Protein or peptide: mS68
  • Protein or peptide: mS73
  • Protein or peptide: mS74
  • Protein or peptide: mS49
  • Protein or peptide: mS50
  • Protein or peptide: mS52
  • Protein or peptide: mS53
  • Protein or peptide: mS54
  • Protein or peptide: mS55
  • Protein or peptide: mS57
  • Protein or peptide: mS58
  • Protein or peptide: mS67
  • Protein or peptide: mS69
  • Protein or peptide: mS70
  • Protein or peptide: mS71
  • Protein or peptide: mS72
  • Protein or peptide: uS3m
  • Protein or peptide: uS5m
  • Protein or peptide: bS6m
  • Protein or peptide: uS8m
  • Protein or peptide: uS9m
  • Protein or peptide: uS10m
  • Protein or peptide: uS11m
  • Protein or peptide: uS12m
  • Protein or peptide: uS14m
  • Protein or peptide: uS15m
  • Protein or peptide: bS16m
  • Protein or peptide: uS17m
  • Protein or peptide: bS18m
  • Protein or peptide: uS19m
  • Protein or peptide: bS21m
  • Protein or peptide: mt-IF-3
  • Protein or peptide: mS22
  • Protein or peptide: mS23
  • Protein or peptide: mS26
  • Protein or peptide: mS29
  • Protein or peptide: mS33
  • Protein or peptide: mS34
  • Protein or peptide: mS35
  • Protein or peptide: mS37
  • Protein or peptide: mS38
  • Protein or peptide: mS41
  • Protein or peptide: mS42
  • Protein or peptide: mS43
  • Protein or peptide: mS47
  • RNA: 9S rRNA
  • Protein or peptide: Unknown Protein
  • Protein or peptide: Unknown Protein
  • Protein or peptide: Unknown Protein
  • Protein or peptide: Unknown Protein
  • Protein or peptide: Unknown Protein
  • Protein or peptide: Unknown Protein
• Ligand: ZINC ION
• Ligand: URIDINE 5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: SPERMIDINE
• Ligand: SPERMINE
• Ligand: water

Supramolecule #1: small subunit of the T. brucei mitoribosome in complex with mt-IF-3

• Supramolecule: Name: small subunit of the T. brucei mitoribosome in complex with mt-IF-3; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#63
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
• Molecular weight: Experimental: 2.4 MDa

Macromolecule #1: mS48

• Macromolecule: Name: mS48 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 201.811188 KDa

Sequence

String:

MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL YQAGAHGAQL SNPRSPFTAK VLGDELMQVR DIKSVEHCKA WFDRLQYLIN LHYEAVGDIG EFKSRHTRHV HE FFVAFHD ALSSLDFRDT YLFDQFKAVR PPELSDLFGI FLEMEANYVH EDYCPRCSLP YSTTRYCGEG DVDTPFRKHR GRW APHQKW GREWYDVVAR RAEALWYRAT EDPYFGTPQH TQRQAEALLK VYVQAKQRGK AIDFMNKLRG SVEYLTGDIT ITTV MQESY DALLDTTPHP HLLTNGFTLE SDAAKYTGEV NKAPLSPLQF RIDMEMNKYR RQQKEEGVVR VPPALWRLDT SAIVP YKVD SKTRRIVNWR EVKEGIEKSF LSVGLPKEAY TGNEWREMLY LHDVIANREA KAAVLEQQQK LDREKLKARE RASLKG EKS SNSASNGIGI IFPDKDGYQV FFVDEASLRP FGIGASGTLF KAVARVYPSP AAVPYDDPVH GKQFLIDTTD ETCHLFG GF EHGDRLLLRA KSSGGDVDGG NNSNCRNTGS FTDEEEEVIV MGVSTEGSDA ERMLCAMHVD TGKQRERGIV FLGTDCID I RERWESVRYA ASAYVKGRVT LLESQRTARE EFMGQVVGIR DGVLFVQWRL LKGGGSVLDR SVAVPIGDST QVRELFVET KVLGVEPLQS PPSWRTPFRN DFAEERLKEL QRAPFKREKY VSLIQGKYTP KVKKFGYTQH TTVDDFETKE YKDRLLSKQF FQNPQAFEV VPDRHERSVQ FGGKWEYQRT HGLPTVDRNE LENGWSEVEP ISDGEMKVIE QALRDISGPR PGNFVKPPSK T KSLQLSES WWEPLGYGWE QHNEEQKALC DVTEQRLIDG SNLPFGGKLP PFGTSYGMGE RMRAIIEDYS KGFGLGPHGH SP THDTIHY NTLNAEGERV RDLGYTDALG RLFSEKLGDR DVHQWAVESC ADGEADVRQL LLSLHEWRER GRPPSLLLAN VLS KYLEEE IAAFNKGIPE NAPKLQLETS DGTLAHSGSG GSRSGTMWAD VDPTTFALHQ ASQSTRSTRC DEPFILHLVK RAKL GECVT NFTDTAYIAH LESSVINEFH LALTKLVGKG ISPTLLAQKT GQLHRGSVRV GGNVVPFVKS RELSRLLERM GLTSE SIAV ITRGLANCPE QESVGDDFAV PVSLILSWDG PGSSGGSGSG DRRLNTTAAR NEQQRKGSAA LSSAIRQLSQ HQKQRG SAS GRGWQNEDKM VVHVLEELSL RNDGLVMDIQ YAIRENKKNR RLRWEFVTTL LPVFGGNEAK VEQLYSDYCD GKYVPNI TV AVEAFIAFLH NATQHPETYS ASDYFDIDDG KSSTASGTGD QPSAGQYTTL KLLDPLEGPF VFDNVKVEYI QTVERFRR H GIRAGPVMAP ATGFIAANCK SLNYFTRRQE EVVYVTNDSD QGLRRSLENS AYHKTIAANP ALQYLLKARR GAALVETFN RFFYRTMPML NFYQNVLKHY SETIQPLRQA AKSSTRGLAR ALESERSAAM EEFRRNSERY WRNIIEGRSV EQVVLANGES NRRTVGGGA GEQLQKPQGN RDAVRTNFTG EATDGSRKGQ RQHQQPPLSQ QQQHSRGEGE RSAVHTSPRQ GGSRGMADLL S KLNTPKTG SGVKGSTAKN TGNNRRGPKS

Macromolecule #2: mS51

• Macromolecule: Name: mS51 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 95.080992 KDa

Sequence

String:

MLRCTVVGHH RSGKARAFVF RDPSLRMMRA GSGYQQLRRM GMPMQVGMGW RKVDSFHANT QYQHAWPLLS HDDLGNSDQS NNTKNIMYS MYMPKRNKGT APWFRGADTY SVKYCEQGRY EYQRYLMINR FPSEYKKHFL SFLSNIRMSS GSATIPQEAL H WLLRMIVD NFNPQHVHYI AAMKTLQSAG ELDMARDVWK IMERQQTWPC TATICAYLDV CVEAGEKTWA MEAWNRYCTE LK FLEPGEV DPKPISRVPF SLTREELLYL PKWKKHFDHD PNLDVMDLNR FNRTREVYLR MAQVMLAGGE RNAFQHFFTK LEE AMLNKP TPVPEPPNPH LVRRPRWAPY EHCKSVHHSP WRLQNNGRAL ALGPPVTIED EMQSRFFSND QFLVHSVKEV LRIV LQEHK RAHPTECTRC KTEAFFYKTK DADETLKFCD DLIERLFASL GVRLSNLNTS SLLSTILEVF RVVGKESGAA LLQRA NEFL ERKASLGDAE GSRENLTASN YLQVLSGFAD ESAFVYNTKK DGTCQYKTGF DPRTTMRHLA DVVQEIAGNP HVTWAA DMH LQVVETMVGC GTMKANDYFV RNVLRQFSWD SRFLEALYVE YRRQDDVDMW AELTKRALVW TARYNAPASE RLRRLIE DD YDTIRVQTRT FRELAVFQFR DVEERRHSRD VVNELPNPWY DYVAHALPFP DRDAGYPDEY GDLGQWRAPG GPGSPVRG P GYYAPPMEGE HQRGYTAEWR DLRNPMRPPE FPTPWERKYR QYARGQHPSY DMVYAGPMPE IFPMRRDFRK PTRWDFHDI EKQGKYRTSG PY

Macromolecule #3: mS56

• Macromolecule: Name: mS56 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 47.306836 KDa

Sequence

String:

MRKFCHFMAN CWNSARSHAT YGAVPLTHSQ VTSVYATDGG KVDELGLLEL VEERIFSWKL NKWEMRIPPN LPNDQKELIR QEQENLKQI LSEWRKCFGA LNADILQISS LTGVPKDVVR EKNRTWLQEE VAKLRWMGEV NKAALLRDAF MRLEAFGSRD F MFMERLCC IYGLARQGTF DEAFTNYITE DPVTNDIFVD ERNPFKELVA HIVRNYSQID IIYDFLGFNY SEGYRSSLRR YM EYLQCKT AENVRASGRL VTGDKGEHNI LFDYCVSRES LVSGDSCQGI IDFLYINGND VTLIIIASDN PWLRNRQLPH RRQ MEGIAR RVCFVLGIPP SEVRIRNLLL PPTYLDKGSI VRLNDIVFRL SNEQSNLLIP WLTNYNKELD PKDVDYTALA KTTN EEEWL TL

Macromolecule #4: mS59

• Macromolecule: Name: mS59 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.395676 KDa

Sequence

String:

MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES AVRSPAYREI AKRQLEGGAA TRLAGISAAA DVMRRMGLTR RPMSSYFELY QRIVERSEAM TPWGFPPLFQ FE ERLSLEP RLKFFSRASQ QSLERRRRGH VMTPHTTLHG RRIFWIPPTW NRAGRFLGPH VTLYPGMTPD

Macromolecule #5: mS60

• Macromolecule: Name: mS60 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 34.546641 KDa

Sequence

String:

MEVYRRITER VRGALWAHHG RLMSERQFHR ILAFVYNNKY EYQIRFDRME VVGREWGGRV EVVTTPAGFL KRVRVNPCVE DLTLYQQQQ LILSAYANAC ADGRKLMEQA EMNIYKQFLK DLKPIVMGIR DNPEFYTVSE NSIETIGGVL QTGDSNNLCH T HRTIPAAK ARQPADEVRA RQEWEQKWIN SPRGQSWALT LRGKTYFALH GPQYRPRGAP GAKKETLPLD IPAPYTAMDE KR LLKKNWM AYLDNKHVAE VMWTRVKIAD REKRQRLLQE TGRAWHRPIN EEAINRW

Macromolecule #6: mS61

• Macromolecule: Name: mS61 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 33.48234 KDa

Sequence

String:

MLCRTFLRQF RMSGGDMFVE YKVLSRDHRR SIRVEDAIVD PTFKRTVLPL GWLELLRSPS LRLPTGYFVE ETVHVSLPNA TSNGGKKEA RPQKGGFASG SPSVGRNEAN AIIAGPVVLY ITGQSVPVVL NPYFVPEGTW DMRTRDGELD LRLGMDAIEQ C TLFSELRP GGLLYGKLPE NPNVRRNESL RATLGRYGMK CDLAESPLVP RPWTRMRYMF IDELQRGPKL TEFVGHNPRN GT PWRFSQN TKYFRIGIWR DTIRRNDMNE GLHAHSSWQK SPQQSVPEVR FLAPYP

Macromolecule #7: mS62

• Macromolecule: Name: mS62 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 32.0681 KDa

Sequence

String:

MRRFCTARVS SYVKRAPLLL PRGGRRWRSG AGTTADGGGE KEYIADSFES TAGSNAYAAM ELAAEARREI HELWLSAETA LEREKRVQQ VAALIEKYKL DPSTPREADV SRGLGDAFDR LLLLCLPLGK TDAKGTDNLE RLMHLAGRNG RELSVRTIQH L FARTDSFA EALAVFYTMR RCHVAMNMEA YYSMLYSLQR LEEEGWGQHF RNEYEENGAP SEQAMDFIVK GISNALLPEN KP WLGRVMF QDRNVPDRRY DTRDFDELDT AWTQRYKSGT PAGAH

Macromolecule #8: mS63

• Macromolecule: Name: mS63 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 31.922178 KDa

Sequence

String:

MLHGTPVRRA SLRYRRPYWM MFLKGVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHMLRK ERLDGPETP LEKYVLEWHK KFHSFQGTER PTPDDLHTAL DLVERPLDLS YALQLLGQCR NLNNIRFAKE TFLVFLEACL R VGRRDCAE YALEHAEPLG FWFIDEDHRR YLQGEQTWYK LSPLDNLYYP VEENAKLNEG RKPITRLSPA TESPGSGTAI SD GEPSTDV EGETTVDDEI AQLEAELAAL EREGGGK

Macromolecule #9: mS64

• Macromolecule: Name: mS64 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 30.742207 KDa

Sequence

String:

MLRRVPRVLQ KGERGLYFPI NHRIVDHRIA PGVTVEETDV QSRYRRELRT NFATGETRQT IAPPWSARER PTHFFSLRLP SRNSVTLRV KEMHNHLLFS HQQCAPMLVS PTKLHITLGV MTIPENSREE LVKNIQQSMS ETFACVQPLQ LRFRGLGTFD F GRVLFIRV VPEADFGRLE RAVCDIRRRV GGVLGVDLKG NPHDSYVPHV TVAKIRPNQR QQLGDTIPQS LWADYQHHDF GD VTLSTVD LCRMKGSPDG YYHTEGAVHL S

Macromolecule #10: mS65

• Macromolecule: Name: mS65 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 30.617381 KDa

Sequence

String:

MFSESLCILS RRFRYNTKFP ALVSYNKLPW EVVNHETPQF HMHVAPHYEQ LLTLAASSPV PHIIGSKHID VPREHRLRLL PGMLYLLDG DTLPGEFTIN RVLDPTALQY YGRLSSQIVT VEAVRMLVPD DLRLLCNCIT FKGPLHLPVA PYASLASLRG A SQGGTTGS ETGSNCFTLY HFVRPNRPPK ELQLEKYYIH APCVAPLSEF ASNSDERGNW RPRLQAPKRT RRATPLPAYR PP QSYLMGL AERLAVVPGG CFGRRSLMWG HWF

Macromolecule #11: mS66

• Macromolecule: Name: mS66 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 29.960182 KDa

Sequence

String:

MFNRNFILRA AEFKQKSRWS SVWPNMRYGS MFLSYSVGRK LPMKGVNWVT RDSNRLLNFS NRYSAVISDI DVKRTEEELN VALSDIRWN DHRRIYWKCS FCGSSYRKNV SVRTKYHAGC NFCKKKYPSE VRGEQHGSPP VSQAAPELTE QLVDNGKRDN L ATLAVTSK FYAEWTCRGC GGSYRSTIRS RTGQVEAGQC TLHPDIVAWS AYCPSCSWRP NMVPIAEEVS RSGQFLGLER SM EELGGQR GKQLPIPRRK RLAC

Macromolecule #12: mS68

• Macromolecule: Name: mS68 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 26.472807 KDa

Sequence

String:

MRRGALSLLK AGLLGHYQQE AFEARKRFEE STTYPGPIRA ATPGDTRFYS GSLESILHDT DRHYWRAVTD DPRVQHLIPL RIRFKIFTW VTSGWEQRMQ VVQIMAPKDS TIAQVKDLVI VENQSPYLCV SSFHLAIDGK ELDPQKTLGE YGITEQSQID A IEQNDHLL HRDDERPRDW TVDEITAEDV KRSPYKEMEM QPLQNLAPRY EARPKGYFGR TYYSGMKQSS

Macromolecule #13: mS73

• Macromolecule: Name: mS73 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 11.360885 KDa

Sequence

String:

MLRRSILFRM KYADLELTTR GEFPHGMKEP AFVKKLDQNI PWYFSTYRSM YHWPITGDNW SDLNEAEKHH DLHMFYTLAW WKLGEGIFG VDEDS

Macromolecule #14: mS74

• Macromolecule: Name: mS74 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 8.203408 KDa

Sequence

String:

MFSLTQTWLI AHWYCGHKFR HRFMRDKRFH PSLQASHDAR NRFSKRRHFK TNRWNYQQAY RDMP

Macromolecule #15: mS49

• Macromolecule: Name: mS49 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 137.234125 KDa

Sequence

String:

MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE LLRSRAAEYQ VANTVHESES VHPIQQLYNS GGTSRSALKQ QAVADRYSIA EQHSPLPLTT GVDRDALDEA QR TKDRILN DSFTAENLLI THGLREKEKH DFTILQRTVR IPFQGYDMDR FLAQQKGTPY GAQQLPPNVV PSSMEEAQRT LRG SSATAT PLVDAVAQKV YARNTVVDRP AIGEQLTEQI INIMRASRTT AEQQREEERA QRFGLGRQGA LVQDGGPDQR TLKK HTNDE RIVDAMLFQQ NAYRKTPTDE HWNPYIRRST ENGVGHLLQN KFDIMRREDR LSKGEQDLTE RNTIHYGVPI QQIVD EFVF RHRNARGERP LDYFKPFPNF RALRLNRMYR DVEGFSLMKQ RPEFLEWELF TRYRQHHQQR RRLALLHGLE PVANET AQE RDTRRHRLDE ICERTPFDER EMRVNDDEMR VSVETLRSWF GVYMLPSPTV VNAVLGGSAS VNLHLYHLAD EMGTADT RE HVLSSRYLNR LLLLESYQNR VGRGFMNHVV GRAPEPVVPH EQPQEVLRHF SAEERAMYEQ HVKEQTSRQL GEWERAMK R RRWLTDHQQY GHVVSHGLET SVVDLSHTET GAVLTVSTKA YEQEIEAVRM KTNATIKVDG MVYNLLPNSE RRVVPLTVQ LDSGEKIDMT SEDFDRCELE AFPRNLNHAL NYGIANYAYN RGNYVETQDS IWEEQTASGQ EGWSPATHAD GLREGLPVRA RRPIFSSSA EQRIAGGPQR AVIIQYHHQP FFNPEPRLVK VAFQCDGTIM EVPISDVMIW QRRYHGPERT VGDESRRYNP A AMRRYVDV TDPFNEKTSN TEHFLDKYEP KRNADTVADK YRTTKQITEI DKWTRYDSAR ADNYRPLSIS HRRDYIRMGY IP RYTPWEW IAIQEADQPL IAEQIRQDNI GTSYFFSLNR YWRYKASPHG YIRHFENEVR DLLQYVDGVT PWKQAQKIRT YWE VRSHHP MPQFNRPEVA MHRNTVGLLP AHMWETDKKT GKVKSVKDSV RDYQTKTPYP KWVQL

Macromolecule #16: mS50

• Macromolecule: Name: mS50 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 131.71925 KDa

Sequence

String:

MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF DDESDRPETR WSQDTFFREC AYLSEKFLKE KVSNMQQFEA ALKRASPEAY LAFFDAFQQQ TQTQIPLPSP SV WHYEGER RKQWAEKFIS ISHKAQAFFK DVLSEDVKKY QEVPGKLLQK VKPVLADVGK ILVKRHERWL KGRVWTSLTE EER EAYCMK EVKRQQMQVE DGEFDPMMED DVDDTELEEW QREHDAIMKL MNSPIDGLHF TTLELWLHTM RCEELETEHI YTSA RVRAI QVAARKKLYD TTSYEEVIQA VVESIARGTL DLGAGVLRPH FNEVWCQLNY AKFGSSTITQ HTTTSRRQLL FFHAG SLKD IAATATLYYA TKPLSNSLDY ASPYKYRRSL ITLCSNYGVE TAYTTQRPLL RSAANLARAE DLIHAVVTAA AQPFGE RRR AATRDLHMEF QRLAVPVERV IVANPVSALL ESGADPDEKP VEGEKVNMWP LGAKRVVLYK WSAPNVEKLK AMESDAA SA VSGSSLTAKR LREIQELKRR GFLEVSLWRR VTAQERKQRN EIVEAKKKQV EEVVRTVPSL AHLHQYATSL YSRIEERV A FPTETSTVTE TTNMKEETNK PLEDSEWEFA VLLDDRVLLN KEESVELYLP YRDANGELLA QGEYRALVRA FDLEANPNL HPAYCSVGYS ESFQVFDALP QLIAQFFRVK DATAEAAGVT HIPAADFTPF CAFLRDAGLD VPLRCEFEAG QAVTTDGDVY MDYFLQLLR GEAFHQSHAQ AGLTEAQRAI EPLCRAHWVV HHPGADESEW ATARRSVLDH AMQHEREWWF PNEMLDVKDV V TGSTNGLT PQMYPAAVRY GVELCTVLTA EGKFVDERGS GLSARCVVNG TGAAESVVFD TANCNGTNTT SVEDALRVAH GA LRSAQDR HNTLAAFRLG PLSKQSQVLL FCGVNAYEFG GKYARTYAYA FEKAKKELEA TAASGFMAPS LSHEDTERLS DQP TTSPSV DRFASTTHPE QRKAQFVPRV GPGSTPLEDP AADQKSEWS

Macromolecule #17: mS52

• Macromolecule: Name: mS52 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 85.012258 KDa

Sequence

String:

MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ SILDEVARAQ GMLDLFERQP HFPAIHIDRC SRFHLVELFK EMVLERSLDS NMIWEKALLY RAILSERKPS YP TSFHYIF TAVEDTVFAP TISTPMEMAG SGGEGHDRSS HPLAAKCPTL EAYYYYVYLV KKYYIDNAVE AHVVLRCHRE PNA ADLLFS NPPPKDDTEI MKAVELLRNA DIQRGVAAAA AVSDPTLPPG GEGSVIGNSD NKKNSEKTSE GSRGRPARPP VLPG AYPPI DMLWRCEENL PLLKVLLFGE FNLIVSENPF VKFPSAHGFL TRPYSTDSSR TLADGMSLAN VMAEKRGHLL PSLPR NTAT SIDARAQDIR RLQQKHHRDD IVSFQKLLRT THAEDSPSAF SSYSDWSYFN PRAVRAEERD RLTRKAVEAL KLYDSA TND IYRHSFEDVQ ACHTQRVTER DRTMPPYLPT LPHFVAIIKK DPHISFLLHI GLPDRNSSEE GSAKHKELEK RIYYLAR AL YHTALEYHNE TVRRVNRQKV NVAASLLDNF VEQEWTTILR DKHDVTDVTK TLNDTQNDKK QLARRLGRYM LFANRSLD D TGFPTDARAD DYTRWMAPPS VGKVSL

Macromolecule #18: mS53

• Macromolecule: Name: mS53 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 75.338914 KDa

Sequence

String:

MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQVDN YFRRSVKLNR DGTLPTDFVH EAPLMRKIIR LAHRGHLKAA CEEYRRVTTV PPVEVYRALT AC CVPGAKL ADAVSIFEDG DSKLFYVSRD GEVLHNLMRC AIAARHRARI MWVYNVMRGR FYENVVVRAE VDLIWRYRIA MIA LEYLLD HECAEEAAAI YSYLVEEELL RCDVHVRVGL HMREAIAAGK PITLNNDVMN ATSLVRDATA VAPEVARELQ RRHA QTLQN NAVEAVGAEN VREGSAPWSI LGPLTAIGPT AEDTMVWLQQ HYGDVDVMSI MRWARFRKGK DLMAKDRPQY LARAA AWIE LLSKRNREME EVPLTYMRKS KPLVLDTNSN VRVAWQTPLM RSGGPPRLLA REEGYVFHHS NSSRFVEETY RHPGES LQS RYLALQPLHT EVSAKEDFQR LYYQAQKHHK QQERLLPVST AAIPSRIVHH SVMSALHGVS GKGEPANRSL FT(UNK) NKSNDS HSNTGVASGT SACTDGAGSA TPEF

Macromolecule #19: mS54

• Macromolecule: Name: mS54 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 72.046031 KDa

Sequence

String:

MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY NNLAMEEVER LAPHRADRLL KEQMKRFRGN LQQLGSGLRP TVYCRYLHTT HTAAVLLEEV HNFLWELVPN DH PAMEIPA LQLRVPFVAS VLRRPSVNPG VSLASVSRAE FGDTDVCAVF LAAAERMVDA DFDDQRPVSE RRLFLSLLTM ISY SGVLYT SDLMAQLMEM VKYSNNDETR DSDAQRVLRY ALRGSSAAQD SASRTLWHSV EKVADCRVVG RYIGARNPWN PIRV CFDEQ GVFKAYPIST TTTTREVSPP EGNGAVTQEQ RASCVEGRTL EALNMRWDDV RRLIECTGVL VTPPSERCPQ QQKME VFTG MAVYLRTVAT GRRYEGGEDV LSDGAVATSS CEQRRRGTLF AEGYDFDVWV RLFSLVQEVR HDMEKFMADH TLQCVE PEF ECWEALLVTL RCALDFCVVQ MQGGGARGTE REVVERLFRD VVALREELIE ESRTRFGGRM RVLWLQEA

Macromolecule #20: mS55

• Macromolecule: Name: mS55 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 66.67668 KDa

Sequence

String:

MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA YRWFDLALTS LAVDCGGRTQ PSHFVPYLPQ GTAAASHITN ALMRTLLTCG YTHVAAIPDS VYDRMGSMGI SP TISTYEL VMLALSLQGN MVEAESILSF LRSHHSEHIT VESFNALLLG HREARQFDCC DAIWQELVDR RWPRASPLTA ELY LRSIMD HANTPTSEPL QSFANINVVE KKKVPLVLAQ MDELGVPRTH LSRVLMDEVE DSLRKFQTYR SRFYEWGRAV KQFD FIEFR RRNGWLYDLH LMKCTTKQVG PLRDFNDPDA VQGAVATAEI PAFFNERPAW ERPPLEETLY VTTNKERYDD VRGGD IYYD DTRGLHDRSP TWMNEVPETR YDRLYGVNHP DIAKIGIRRH LNVEYVNRKE VVERDAALMK KTLSSGRRLR HRVESS RTH RNAGSLSGIS STAGGGSR

Macromolecule #21: mS57

• Macromolecule: Name: mS57 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 45.956293 KDa

Sequence

String:

MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY CNKTDNSGWR ALFDRCLKDG WNYTPLFDTA QWSFMLKSIG RMGDEDGVRA VLEEMLDVQA DLDRVEARSV VI ALNAVTN ADVYEFVKKY LFNFGERKVK FLRTTYSDLR GHGAGKLRIP LKENDNMYYH VCWHSSIRSP RQFSPRQLYF DYT PSTLGS SSHNPNAKID DIVKDKIEKW KAEGLLPEDY VHEDRVYDRS AAFKNVARQE KWKKMPKILK SKRMGYTGDP

Macromolecule #22: mS58

• Macromolecule: Name: mS58 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.62391 KDa

Sequence

String:

MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGSLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP WMQLVQHGKK EEAAALLKEF EQKGDAAEAT PTRVKDDRVK GTRTELSNRE QPPLASTLKA EDSNPATIPI GS VHPLFTP ALVQIEEAGG GSVSNEAVCR AQLWELSRDE QLFWERVELI RKGQVASIGS SHKRGYADEA AFAKDDTEEK VQL RFTQ

Macromolecule #23: mS67

• Macromolecule: Name: mS67 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 30.096051 KDa

Sequence

String:

MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE LFGESYSDMN VSDVLSPWKG PFPQSGVFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL EKARHPEEGP RQ NMPFGLQ

Macromolecule #24: mS69

• Macromolecule: Name: mS69 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 21.589732 KDa

Sequence

String:

MRRVRDGLFL SHPSSALQCS VAVVSTGEFD HPPFQFRQRH TFNTTPLHDA NRFGGRTAYL REIGPVNIKK QGRRFKKDPR TVQFNVDVW CAQQTLRKRW KQRDWEVIEI PFRLVPREQQ RVIPELYTDI PQMTDPARND FSNIRNKVYD REELQGVLFP A AGAMLYPP LQRVDKQAMT LDKYL

Macromolecule #25: mS70

• Macromolecule: Name: mS70 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 21.247398 KDa

Sequence

String:

MLRFTHRALT ATPERFSVLG TTHPKPKRTG FGRNNKMRSK PSDNVAWYDK GPVEWLPRPV RLTYDHLDQL QQWTMRATLD GRTEEFNRI RDLHREWSQH PLMPVLGDVE PKFPLNLFKQ NHRAKKRFLV RWHKANTPAN WLWMPRGPTV VTPLHRTNPT Q YPENWKQM VQRKSGTGTP S

Macromolecule #26: mS71

• Macromolecule: Name: mS71 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 20.093227 KDa

Sequence

String:

MFHRAFVSSS DLTGCTIALS SVCTQKRYWA KPKKRPKVGQ GFHEKAQKWR EEYLLDRHRA LADSLRAYVE FSTSKRVEPW DARFKPFDR IEKDGVYVLM RYMMEEKLQL CNYHHRPVKR LFCNIGLMGP QITTKARWKP YRFATNPAGT SKAERMYQRD K TVYTHGHN D

Macromolecule #27: mS72

• Macromolecule: Name: mS72 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 19.317293 KDa

Sequence

String:

MLRSTLTSLN TFLTSSVATP PISVIRTGPK WWADPERMVR QKLMYFTLGV DQLPLRRTAV IQRDLQRFHM CKPPPRVGDS TGYKRSRAA QLNTWYRRIQ YQEYHMQHLF TRHVWGLLRV YPGNTTKIQG KADDGYVGYD SVPFHRYNRA PLPFPARELY E RRK

Macromolecule #28: uS3m

• Macromolecule: Name: uS3m / type: protein_or_peptide / ID: 28 / Details: mitochondrial gene for uS3m (MURF-5) / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 9.07094 KDa

Sequence

String:

MFLIHFVHYK TILQKYTFKF KHIFLSIDKY NSLFFNISGI LIWLNIIHIN IILIKYSFFI LINNFEYLII LIST

Macromolecule #29: uS5m

• Macromolecule: Name: uS5m / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 50.147355 KDa

Sequence

String:

MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP ARRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKRGYYDD VYWKDFFAGS DEHLNEPRMG LRGDEMRRRL EEAQTSPAPT TAKDTRRRTL EDVL KRLGK TTRDLGSIPI VNPRVDVGLP THIKRNYQLH

Macromolecule #30: bS6m

• Macromolecule: Name: bS6m / type: protein_or_peptide / ID: 30 / Details: residues 105-114 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 18.861543 KDa

Sequence

String:

MVFYSFVLVM KPRQRRFTSQ ALREIGVAVY SNGGLIRSIT NEGIMRPYSR FRDADNTPLT YARYIILQLD MGEEEMGKVD KIIREHQDV LMALKLNNLE RPVGIRSGNK ELQAAYFPLD TFTRLEEEIN WSPQTSADIY TQLEMNWKEF SRTRWSSFLR N

Macromolecule #31: uS8m

• Macromolecule: Name: uS8m / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 32.766459 KDa

Sequence

String:

MLRQSLLSLA KVYGAVPPLG AAQGHRLLHG KREREGSLFA VANDVKRDER LLRQQLNALL EEERMPMQAR LQRNEAVSSH EAVPQTPLV DLPGVERRRD LPADPITRLF FQHKGDHALY YGTYDKPSVQ DDDRVQIEKR VPRRREENLY TPIYDFCHRI R EATEQRKR FVVVPSTIET RGCARVMHDH GLVAGFRDFH NDRAFAVELK YFQGDSTINV IEPCSYDGRT EFEWSPKMMR RL LNTHGIH NRLVVYICRT ADNRIIDHIH AVKENIGGRG LMMVH

Macromolecule #32: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 50.201016 KDa

Sequence

String:

MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF ARKVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEKY HFT ALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALV KLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

Macromolecule #33: uS10m

• Macromolecule: Name: uS10m / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 94.351039 KDa

Sequence

String:

MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR EHHQHARILS PLEVQRDVGR LEPIPSYAGK INADRSVFPF QWKTEDWYEY EVAKVRNRRF VFENTEEDGI RG SEVTYKI VLEGFWDHHV MKLAEDVCMF LKDVGRQIVE EKLVAVRRLL QGGAVDPELL AAFNCARAGP FGGLDEYDKE EVA NFLRSD LRRLEEQCLS VINRCNVPVP GATNIYDPHT SWPHVEKLEP WVRMAEFWTS SSDTSFTELE MSTAHYEFRK FFRV IICKL PFQSTEFEKR MYDIRHWLHR QTSCEFHTIY RRNVIHDSAV FPTEHDPATP TTHEHHRMFS FALDWQSAPV NRLST DTVH EGESWDAVAQ RLGCSVGELK DANAERETIE AGVVINVPVT ATRRLTSFGA TPLVLPLKTT SAKDGERIRT WEEAAA ILD CTVEELQQCN GHAALTYQKK ESEAGEFDSS ETELVAPLSC WTSTSESEFS PVERVHANDT LVAIARRLQC SEEALRA VN DGITDVSGLD FVRVPPEARR PRRLVEPQLR PQAATDALLA RTIAEEETFK LKSIPHLPQN AERFPHEYHT PTSRFPPT P SETPATQDWM AYTAKYLDKQ FTISAEPAPV YNVNKLWPMQ QIPGKVDQTP FEEDQTWLLH SIPVQQLEMH HHEKDLQDL PFINHEQFPR SLEWNAP

Macromolecule #34: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 37.92591 KDa

Sequence

String:

MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRS E GSTDNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLN GPKMPRGVFK

Macromolecule #35: uS12m

• Macromolecule: Name: uS12m / type: protein_or_peptide / ID: 35 ; Details: Mitochondrial gene subject to RNA editingalternative start codon; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 10.421635 KDa

Sequence

String:

LFFLRMWFLY GCCLRFVLFV LCYYMSPRLP SSGNRRVLYA VFYLYNFVWM LRCFFCCFIG LVMSLFIIEG GGFVDLPGVK YYTRIVS

Macromolecule #36: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 19.900014 KDa

Sequence

String:

MFCFSRGLWM RHIGQDVPKR HTHFVLESRL MYEKSFRDCW LHSVCRAISQ LDEPLSKTVV GTHQKMLQRK VTCFQYNQYG LFKTPYYRL ANVDRYHAVQ GVAGTREWVP YVNVSYWTMN KMVRGGNLLV HRVHYTGWGT DSHLKKGGWE HRWNKVLQRN V LQYSRI

Macromolecule #37: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 50.401191 KDa

Sequence

String:

MFVRSFCFLR KYGSKSKTPD LKTSFTKTTN FRKAEKISIT TTDGEIVKGR GKIRRRTHSA LKEAIGAMEH PAIWLWYPWR MNPEPPTPH MPQRRALKNV HGAVFNDLTP VQKKRQEQML YGVNIPETRQ MKFEEQHPLL AGALRKLEGQ PKGFPFWYRK Y PTRRHAYE YRFSIPVEML DGYNDDVKKA LSKGMMSIQE KQFAQEAMYM ERYAEHDFDT TSPAVLAVKR ALKCRVLRNH LL TNPHNNI IKTVLANTER KLNHALRRLR KVDFKKYWEI IRDHDVQDIL QPPNLVTYRQ GSYWKYDWNA GLAISTNLAD VMD PRGLNG CVETGRSRSE VARDLGLSYT RPLHENEKKQ LSHQAVYYER LAKFKMEQPE AARAMERERF VRKFSGMFVK MDIR SGAPD FPSTYRRLLG TKVVRWASKR HGPN

Macromolecule #38: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.077805 KDa

Sequence

String:

MQVTETVLAR AVIKRRSPQL WGAPGAPIIR MRGHHVVWKF QSYDLVVEHT HKRRNSDIRL LHYLGKHCPH PQKSLWSPDT PVAQDRHLF MLTTVDIDAF KYWFGVKRCR LSMKPWALLA KAGLLPPSLT QNSKIMPKPL FDKESLMRYY LANRKDEDVM A REKYLNYE NSMVKTEEER AAERPVAPYL

Macromolecule #39: uS17m

• Macromolecule: Name: uS17m / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.632727 KDa

Sequence

String:

MLRRSLLAFP WWNFQTEHRQ RCVLMYGGAR TKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLSQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV ATNHMVYYPK FNQRVSRTKR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN VEGRERLKLG LKAVPPPLFG YPVSRRIVKL NLTSTEGTQE KLAAAIQEHV QDAYRFSGPT PDQPRNRLAD PV TFEDANN MIAPNAPAAA ALDASDSPPL LDRGEYTEVE QDTRNKKGDD YWMNLQPKEK YDFKSFKKSP

Macromolecule #40: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 36.988691 KDa

Sequence

String:

MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKRTVEHF AYNWVQTDRI RRYFRELEAV HTSGSASARG SG GGTTAEH KQQDQFYSPE NQPISLHRNN ISYMEDVGRS VKNPTVPGLM STKGMKKKFH NLYSSTSTKR MGFSNPTLGI KKV

Macromolecule #41: uS19m

• Macromolecule: Name: uS19m / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.364604 KDa

Sequence

String:

MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG KFFVSKRQVY TLQHATSGAK VRVKSFPSIF EFNSPSRWNI GKEMNTLTKP RMDLIDEQML TKKQRLDYVR AG LLPK

Macromolecule #42: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 23.087889 KDa

Sequence

String:

MLHTTRLWLG GYMMYHRKAM GTMKYSKWKG AHGGISHFYG RTPMVEEVRP NEPITLVDRR IMHYVHHSRL RHFQLFRSYQ EKSNSTECK LREGEMLRRR WHRRLQKSFI AFMQFKTMKV LEDQARLVNT YGQAAVNAAL GDPWNATDNV ARERKSAAVR R QVRALPMV NVVPKHVATM KQIHNDRFNY RWRVN

Macromolecule #43: mt-IF-3

• Macromolecule: Name: mt-IF-3 / type: protein_or_peptide / ID: 43 / Details: residues 294-304 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 41.086285 KDa

Sequence

String:

MMQRCSTSLS RLCFRRLLRT PLLVYSIPPT RDVPSGIAHC PLSCSMRMVT SSNDDEFVFD PTLSIQKDAA IHTAKKSFET IVLEYVPAH APEEARQKVK SYLTQHPIDI LITQPKVQIT HLEDAESGAE TKVSLSPCDL PEALQQARER GMNLVQMGAR G DVAYCRIR RESTRILSLI HTELEALREQ EEKQQGKGRG GVQAAAKMGE LIDHTFRDAV DAHFVGWRSK KIVEDIRRRH PV KLTIKEF QSPECAIGKL REMCQAMQHY AQEKVIYHHF TSIVANDREA SITFAPALPM AKSDSWKHIK YPGEKEWTNA LRR MEDACR KSGRYGTYAK SNKLKLRSLG QTSYRVDKYG RKMD

Macromolecule #44: mS22

• Macromolecule: Name: mS22 / type: protein_or_peptide / ID: 44 / Details: residues 20-37 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 73.064141 KDa

Sequence

String:

MLRRAYIQRR YPFNKRGPRE HKSWKHHVLT EPPKPLQWRD PKVWTRDLSV MKSFDAPQWD LWQSRPRSED MDEALQPFMD MPKSLKDRR YDIPWWANPF GAWYLQNILS LELLKLKSKT NAEKIATYRS YMRSLASGKD NTMSDDDVIR NIIKERWKTL E FGDRNAGY PCTFGDYIQF LNEWFKSLDE EGMQRLREHF DRRIRPLLAV MSPVDILWLE ALTQNSPHNK EQLQRKIAFQ TS LGTPEFF DMSKRLRYEI NEDYKVRDEL GPELFALWSK APERWPPERL SKMYGLDFTL VRKILVWHHF KACYDACVEP DWS LPKRLF ALEWIRDVRA RKHGLFYGKM RFAEQKITFY SDRFLFRDLV NRREASYANV WEMDDPYRFL QTEQDYEDYW GDNY DVYRR MFPEMIGRTG EPVQQYGQMP IWAGPHRQHA NKSEHNWMFA EIGVNVGHEA LKKLELDPTN EKRRRFVIRQ PDGTL RSAK MSEMRAWYWK EEWADFRFWA PQMEWGIENT PSQEQYQEHV PDTTDADFRK QRRIQSRPVK WFYESHYTRT GSFAGF QPL RFMQRRTERE VRWPDVINAA VQIQKRKPAA YIFKAIPEL

Macromolecule #45: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 36.855578 KDa

Sequence

String:

MRQCVVRRYK MPKNMGVAPR FDTWNEKYEP WEHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPHNASEPT QDDTSTLSP ERVALRDELA RKSRLVASEG MRYYNIFWVR KPLDTMEKEY YELKRRGVDH GEAIRKVLQG FYSGLAVKKR V AAIQAEEA KLTGRFITMR EATVVLGVLA KLHKEQLTPH QVSLLAKEQG ETTQSGAKLT AIVSRTQPHV NKEASSPATS EA VGSSTEE SLSADALASM LSEDGEQSAV GTRYQVEVKE TANDSVRQLR EKAEDQTGFP DWYTGESPTY SGTSACSRDG FAL MKANK

Macromolecule #46: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 46 / Details: residues 221-248 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 51.164738 KDa

Sequence

String:

MMRSSSFCRR QIRPYYNLPS KSEHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRRV YKRQWLESFR VNADEYIYKY NITKAAQLA QWECEMKEQE KKRIEARQMM DGRQALKKKH LDLLREFHER QFFFWYERAS ERLQNMNLIN YVPHAQLREH I DKELDKYV AGKNEPYPLN FVGQMPFLED GDGNIVEVPE SLLSNHMAEH PDSTAKPHEP HTSSSISEAA AFEERMLRAM VS AKEEDLK EWLGDDSRAL SETIDDISRE EEEREADIRV ARSMEETDAE REVSRRAYIE R(UNK)KTGSRSIF RPPTVSEGA GGTPSAPAGD ANTPMRRRKK GKLDRVHALQ AHQDELLAKL SSQGLKEGVD ASSVPERGKI VQSRGRIRDK AVIPTHEVLM QKPELAAGS TPGARIQTKD MVDKMYHRGK YKKSGSGDKS DGEDL

Macromolecule #47: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 57.048836 KDa

Sequence

String:

MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS RNIVTLYVPD AKVWTHGEWC WPSTILPGFF DAPDAARSFL KYFAVANRAT LTSWKLRCTP KDLPTEQGER QP QNLYELC EWGHRAVAPA SIDRQSVCVK FLMDELSEEK KLPVVIVVDG WNLFSHETHF RYPHPDFLRG LASFNESSTD IDL YPQELP RIPASRLSFV RGLNKMILSG DDPNKFFITC TTRDFKPFDG ISGFPNVETD RFANSLDEYA PYDPEKDSHF HPIQ IGNFD EYEYRSFLRF LINSGELAGL GWGPLWHASS DFERKLYKIG FLSGRNPQGV VDHYHQELVW RYDYQRTRQK QYLLK RRME GMSRGASSGA VGVR

Macromolecule #48: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 21.244104 KDa

Sequence

String:

MVLRRWFPLL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMRDMRPGY GQNLPDYIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFKVLCS S GAKNPPSG WEPIPDATEE EED

Macromolecule #49: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.72818 KDa

Sequence

String:

MLRCARVALR ADPLNGGSSM TLGSKGSKLS PEPHRRRMPW TAAKEYVPGV VLNARDKMVL DGVQLLDIES IDRASQLDPL EVLRAVVAT REYNISTGKN IFQLASQATY NGRGQRFYRK EWQEGTYDKY VTLSAIDFDR DGNKGTAYGY ITFHGETTTR P VQVDFADV PGWYMDFVEE RAVPFTGIVP PPPSIGTDVP VDPHSYRLKA YPYYDAPNPP EFVERLLKDR GVLPDTPTET AD VDKDPTT SDGSVHYDGK

Macromolecule #50: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 98.377773 KDa

Sequence

String:

MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE KVVIPKLTSD EEDRRKLVSL WSETTLQAEA AVTKFLFQRG SFESMLHRII TDALEKMSTL ALGGQEGNLA LE ALKRQTL FKRNDYIQKR LIDVVSNSAY LGYGDSVWQV FFAAVEANEE NLLSDRATTD AIRAAWEGVM REDVVRLPDV TGV VALYLT LVCIRESGRL VPEELKELSS GLEDGVRPGV RKLQQYPLIF LHPTVKRRFV VKAVAEILHN SSSNAFSNML RENG LHDTA REVALCEAMN RNKELAEGDV GDAVGRFVSK GEVKTLLSSL VSGTDAVVRD AVAGIFGIGT TITIDWDAVM QNVDW SNNW QRLATALLSN SAVLSAIVKL VKNAIGAKGM SKHLFTDEYA DQLQLILDAR EERAASRKQR IENIAQELSS FERVDL SCD LLRKLGVDMT ELDTAAAATR NMNVVQRPCI EDGLLSLVLE AVTKRHPNWV KAGVIQTTLK DPFDALRWMM HIFIRLS YV PHAGAATIAR LSRRRIGPIG LEPHQFNVPA ELGFVEQYDN LQYKRYDWQG WYQRMLDVHN RNVSLRCRIC DLQRLDGN G VQFVDMQTER RLRILAQHRV GMGVLKLDAD KYEDQADNVT FGTTKLSELL ADARKAQLGE EYWPSVELKV RKPSGQSKA HYSLIDNERI EKRSRELYEK YRDAKKRSLF VTPMETWLEV KGMQVRKSVD NADEDGYTLD ALQDMMDGDD GDKV

Macromolecule #51: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 24.757896 KDa

Sequence

String:

MKSSDIFFAY RLTPVVFKSR QHDSGVNQYG LKPTNAYDYI NPTNLINFGR GTTFDNLGVR RAGRGEIDSS PSHSGSPVFT QAKLIGLSG EEQLTMCQSE TMALRLCMAK AGKETCERES RALDSCLGRV GHLRRAMSEA CWEFNDWFIQ NVSDNHTKPF Q HRPHDWRH FYAQEKLVRE RQQNGHAYGR RPKQFSFGAR YVKTDGYGKR PRLPYNK

Macromolecule #52: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.2406 KDa

Sequence

String:

MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR FQMKKHRIIK RFRFRRYKLA AVANLPFAKM IRVGMLPELK SSKTKRGDVV DPTLSGQLVS AVKNTGEKRK GQ RTRPKSK YQV

Macromolecule #53: mS41

• Macromolecule: Name: mS41 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.421469 KDa

Sequence

String:

MLKRTLTVLD QTYGPHKSYK YTYMPDPRKL APIETTQRSE IVPQSIRPPT SYVPNHETFL ERADIHRLRP TSDFKASFKD WNDLFTCDK RQLRVRGIPR MTRDAIRTAV QAFQNGNPPE RFDTKEEWLY YKQFKTVDYS YRVIPELPEK YRPHQSGIDQ A PLPDYREI NKMPQWAECE ERRQSKKTV

Macromolecule #54: mS42

• Macromolecule: Name: mS42 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO / EC number: superoxide dismutase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 29.748666 KDa

Sequence

String:

MRLCRRLSYH AYVPKGASVQ MGETFMPSNI ATAVFRETEA AAARADKTKE ETGFFSQPRL NYPVSSGIPA LFSKDQFDMQ YSLFHRDVV ETLNRHTLGT PLEGHNLETV IRKTSFDATQ AVAHTAASEH FNYCFFYKSL RPWGTAPPPR LREALQLQYG T NGSADAMD EVRRIVWVTA ASHQERCGWV YLVWSGVHFD VVEFPHGSCP IASDLIPLLA LNVHESAYIL DYGTSGLKQY VE NYFKACN WPLAERYYLM AIGQVV

Macromolecule #55: mS43

• Macromolecule: Name: mS43 / type: protein_or_peptide / ID: 55 / Details: residues 27-35 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 48.59041 KDa

Sequence

String:

MLRRTVTSLR NARELHRLLP ALLPLLDPPV PTGASVCAAH LIVATGHVSK QREPHMSNCP VLERKIPLSE IASDDNFLKH PCVPCLKHR INFLASEDFC YDFVERCGCG TDGALPPEWR CIVERQFGSM AALVEELVIH ASNRREAGWT WLVYDAQQAG E RPLLVVNM PAQRSPLLLG LWPLAVVNVT EAVLVEAVRA ESSGSVAAAP PPPWCRAARA SLYDTKAAAE AFAMTAPMSE GV GKKLSEI REKVARDAVS RMNWGFILQQ LKKAETYYAS SDRGEKIRVH RMKLEREAVT KAMSGLRDSG ATFLTHDTVE VTT TPSANS EPSSPERVPG VQAKQPQTEG MDPCKNKASA EPDPKCTQLP DGTWQYSYRN GDVTLLKPDG TRVFRKRELT TTAY PDGSM LFQYANNTSI LDRADGVRVT TFEDGTTKEE MIRR

Macromolecule #56: mS47

• Macromolecule: Name: mS47 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 137.928969 KDa

Sequence

String:

MERRRLLHDG RWFTPPVLYG SARRGLKTLD VREYRPLAMP IEFRFYQRYA NHPNRQSGIQ FLTHYNTHQR FRVNKDYIDY MHWGKEQGQ ARLPHRHQRV AFDFNDQLHP TRIGTPEDGG NANHCYPEDG EDGSYAWFAS QDPTFGHHPD LSSSFDPNYR V FSHPEHWN KMFTKRRPGE GDIDLSVLPS RSLLGPLMAH SDTKGIPYFK VDNRGHSNGR VPGVNAPFFG EFDQKMMQAM SR PLNANRT ITGNDGRFSK TIMINEPQCN QALSAKTASE LSKEIDKATN AVYSKLSVLE AAQSGLTDYF CGGLNFEIVG FEL HMAAML RERAAAILER SGAGGLPSAS SATLSSSSTT RRCAMLLEKA DEREVNKLLR DASRYEDRVD DALRQHASLI WRVY TAPRP LMTLTNGKCR GTGCGLSLFA KYCALKDSSE FIFDGPNVGI TPYGGMTRLL ARPETSLKYP GLAEFVVLTG ASLFA GDAL RLGWTDLFTT IPDMTYHIKE WFDTTEHMHN DAVAWQLGHL LETCFKMKES HSSAMERAAI TPTRARWVED AFADQP SVN DIMRTLTEIE QLPFQSTHNT SDESHITPYT LGSVAAGVQR LGEHRLRYTL SPWDITPPED SVVLKHTSEM FRAYVLE RR GATDVVTHRD AERAAAWSQQ RQREYDAYCS LKEAPHPRHV YARLEGCEGK IVSFEFVFEI SCRRSGPQGT DEVLQTTT I SRLKSRILDA LGMPRDRNID LGWYLPTLDT CPIHSDEELM QVLHTDPGIE DPKEKLRYPP IYFIVKRCTL YFSEWAYAV KHQLLLQSPF ALRAAFGLLQ EVRGDGAAEN VMPLAETLGT EFKYFTRLMR RPDFYRVGVH TDKSVEKWEE IKEERRHNIH HTHRPTRPL PDFEEVFERD VEIDGHRFVL RPRWNPRTLQ DVDDSDILRL RAPLTYDMDS TAALHVPTHC GKASRLAGML Q DAGGVQVL DGLGEVDDKG EPKVPPLQSN ASVPTNVSFY EMARHPWEDR ASSWRRDGFT EGSLEYFESQ YRSAERAVYD EE GRGVRNY WPSRDAVEGI AQEEENDAQL LQERLFNTLE TAGTSVEPWA RNLRLNATTG KFGYKTEIAT QEEKIYDDEY YRW FIQPGR HPNPSGLTLN GRKGQFEEGH SRDEELERMW RKVVADGNND FAATTSGMPV YDEDAKNAED SYAAGDDVDV VTTD ANVPV DGDDSS

Macromolecule #58: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 58 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 443.539 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #59: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 59 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 613.749 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #60: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 60 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.74137 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #61: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 61 / Details: residues built as UNK / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 698.854 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #62: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 4.613678 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #63: Unknown Protein

• Macromolecule: Name: Unknown Protein / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 3.762629 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #57: 9S rRNA

• Macromolecule: Name: 9S rRNA / type: rna / ID: 57 ; Details: post-transcriptional addition of 7 U residues at the 3' end; Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 198.442391 KDa

Sequence

String:

UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG UUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGUUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAUAAUAAAA AUAACGUGCA GUAAUUAAUA UAUUUAUAAA AAUAUAUUUU UUUUUUUU

Macromolecule #64: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 64 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #65: URIDINE 5'-TRIPHOSPHATE

• Macromolecule: Name: URIDINE 5'-TRIPHOSPHATE / type: ligand / ID: 65 / Number of copies: 1 / Formula: UTP
• Molecular weight: Theoretical: 484.141 Da

Chemical component information

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM / Uridine triphosphate

Macromolecule #66: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 66 / Number of copies: 39 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #67: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 67 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #68: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 68 / Number of copies: 4 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

Macromolecule #69: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 69 / Number of copies: 1 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

Macromolecule #70: water

• Macromolecule: Name: water / type: ligand / ID: 70 / Number of copies: 3 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND
• Startup model: Type of model: OTHER / Details: initial model from 2D class averages in EMAN
• Initial angle assignment: Type: OTHER
• Final 3D classification: Software - Name: RELION (ver. 2)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2)
• Final reconstruction: Number classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.37 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 31911

Atomic model buiding 1

• Details: We used Coot and O for initial model building and refined the structure using PHENIX
• Refinement: Protocol: AB INITIO MODEL / Overall B value: 46.4

Output model

PDB-6hiw:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3