EMDB-19397: Composite map of the C. elegans Intron Lariat Spliceosome primed ...

基本情報

登録情報

データベース: EMDB / ID: EMD-19397

• タイトル: Composite map of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')
• マップデータ: Composite Map

試料

• 複合体: Intron lariat spliceosome
  • RNA: x 3種
  • タンパク質・ペプチド: x 32種
• DNA: x 1種
• リガンド: x 4種

• キーワード: mRNA / splicing / Intorn Lariat spliceosome / ILS / pre-mRNA

機能・相同性

分子機能:

feminization of hermaphroditic germ-line / molting cycle / regulation of primary miRNA processing / SLBP independent Processing of Histone Pre-mRNAs / snRNP Assembly / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / Transport of Mature mRNA derived from an Intron-Containing Transcript / mRNA Splicing - Minor Pathway / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / Downregulation of SMAD2/3:SMAD4 transcriptional activity / germline cell cycle switching, mitotic to meiotic cell cycle / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / vulval development / nematode larval development / egg-laying behavior / post-spliceosomal complex / spliceosomal complex disassembly / U2-type post-mRNA release spliceosomal complex / spliceosomal conformational changes to generate catalytic conformation / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / apoptotic DNA fragmentation / nuclear mRNA surveillance / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / nuclease activity / U12-type spliceosomal complex / U2-type catalytic step 1 spliceosome / pre-mRNA binding / pICln-Sm protein complex / RNA splicing, via transesterification reactions / embryo development ending in birth or egg hatching / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / snRNP binding / commitment complex / mRNA cis splicing, via spliceosome / U2-type catalytic step 2 spliceosome / U2-type spliceosomal complex / locomotion / U1 snRNP / U2 snRNP / U4 snRNP / U2-type prespliceosome / cyclosporin A binding / generation of catalytic spliceosome for second transesterification step / precatalytic spliceosome / mRNA 3'-splice site recognition / uterus development / germ cell development / spliceosomal tri-snRNP complex assembly / Prp19 complex / U5 snRNP / U5 snRNA binding / pre-mRNA intronic binding / spliceosomal snRNP assembly / U6 snRNA binding / U2 snRNA binding / protein K63-linked ubiquitination / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / RNA splicing / helicase activity / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / spliceosomal complex / mRNA splicing, via spliceosome / RING-type E3 ubiquitin transferase / mRNA processing / metallopeptidase activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / protein folding / regulation of gene expression / nucleic acid binding / DNA-binding transcription factor activity, RNA polymerase II-specific / cell differentiation / RNA helicase activity / RNA helicase / DNA repair / mRNA binding / GTPase activity / apoptotic process / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / cytoplasm

ドメイン・相同性:

Intron Large complex component GCFC2-like / Septin and tuftelin interacting protein / Tuftelin interacting protein, N-terminal domain / Tuftelin interacting protein N terminal / GCF, C-terminal / GC-rich sequence DNA-binding factor-like protein / mRNA splicing factor Cwf18-like / : / TFP11/STIP/Ntr1 / cwf18 pre-mRNA splicing factor / Nineteen complex-related protein 2 / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / Prp19 WD40 domain / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / Peptidyl-prolyl cis-trans isomerase E / : / RBM22 CCCH zinc finger domain / Peptidyl-prolyl cis-trans isomerase E, RNA recognition motif / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / mRNA splicing factor SYF2 / SYF2 splicing factor / Helix hairpin bin domain superfamily / G-patch domain / Pre-mRNA-processing factor 17 / G-patch domain profile. / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / : / Prp19/Pso4-like / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / G10 protein signature 2. / G-patch domain / glycine rich nucleic binding domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / U-box domain / G10 protein signature 1. / : / Myb-like domain profile. / : / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / : / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / DNA2/NAM7 helicase, helicase domain / AAA domain / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / : / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / DNA2/NAM7-like helicase / : / Myb-like DNA-binding domain / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein D1 / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / U-box domain profile. / : / Winged Helix-turn-helix domain / Sec63 Brl domain / Zinc finger, CCCH-type superfamily / : / Modified RING finger domain / U-box domain / Leucine-rich repeat / zinc finger / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Pre-mRNA-splicing factor Syf1-like / Sec63 domain / Sec63 Brl domain / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein E

構成要素:

Small nuclear ribonucleoprotein Sm D1 / GCF C-terminal domain-containing protein / Pleiotropic regulator 1 / Cell division cycle 5-like protein / Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein / Pre-mRNA-processing factor 17 / Spliceosome-associated protein CWC15 homolog / Protein BUD31 homolog / Pre-mRNA-splicing factor 8 homolog / Probable small nuclear ribonucleoprotein F / Pre-mRNA-splicing factor SYF1 / Probable small nuclear ribonucleoprotein-associated protein B / Pre-mRNA-splicing factor syf-2 / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein Sm D3 / Septin and tuftelin-interacting protein 1 homolog / Peptidyl-prolyl cis-trans isomerase / Probable small nuclear ribonucleoprotein Sm D2 / U5 small nuclear ribonucleoprotein 40 kDa protein / Protein isy-1 / RRM domain-containing protein / Pre-mRNA-splicing factor RBM22 / Pre-mRNA-splicing factor SPF27 / Uncharacterized protein T27F2.1 / Tr-type G domain-containing protein / Coiled-coil domain-containing protein 12 / Probable U2 small nuclear ribonucleoprotein A' / Probable small nuclear ribonucleoprotein G / Intron-binding protein aquarius / U5 small nuclear ribonucleoprotein 200 kDa helicase / Peptidyl-prolyl cis-trans isomerase E / Probable small nuclear ribonucleoprotein E

• 生物種: Caenorhabditis elegans (センチュウ)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.9 Å
• データ登録者: Vorlaender MK / Rothe P / Plaschka C

資金援助

European Union, 1件

Organization	Grant number	国
European Research Council (ERC)		European Union

• 引用: ジャーナル: Nature / 年: 2024; タイトル: Mechanism for the initiation of spliceosome disassembly.; 著者: Matthias K Vorländer / Patricia Rothe / Justus Kleifeld / Eric D Cormack / Lalitha Veleti / Daria Riabov-Bassat / Laura Fin / Alex W Phillips / Luisa Cochella / Clemens Plaschka / ; 要旨: Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on spliceosome assembly and remodelling for catalysis, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and SDE2, then dock and activate DHX15 on the catalytic U6 snRNA to initiate disassembly. U6 therefore controls both the start and end of pre-mRNA splicing. Taken together, our results explain the molecular basis of the initiation of canonical spliceosome disassembly and provide a framework to understand general spliceosomal RNA helicase control and the discard of aberrant spliceosomes.

履歴

登録	2024年1月11日	-
ヘッダ(付随情報) 公開	2024年8月7日	-
マップ公開	2024年8月7日	-
更新	2024年11月20日	-
現状	2024年11月20日	処理サイト: PDBe / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_19397.map.gz / 形式: CCP4 / 大きさ: 343 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• 注釈: Composite Map
• ボクセルのサイズ: X=Y=Z: 1.3013 Å

密度

表面レベル	登録者による: 0.5
最小 - 最大	0.0 - 8.985284
平均 (標準偏差)	0.00885728 (±0.093956225)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 448 448 448 Spacing 448 448 448
セル	A=B=C: 582.9824 Å α=β=γ: 90.0 °

添付データ

試料の構成要素

全体 : Intron lariat spliceosome

• 全体: 名称: Intron lariat spliceosome

要素

• 複合体: Intron lariat spliceosome
  • RNA: U2 snRNA
  • RNA: U5 snRNA
  • RNA: U6 snRNA
  • タンパク質・ペプチド: Pre-mRNA-splicing factor 8 homolog
  • タンパク質・ペプチド: U5 small nuclear ribonucleoprotein 200 kDa helicase
  • タンパク質・ペプチド: Tr-type G domain-containing protein
  • タンパク質・ペプチド: Protein isy-1
  • タンパク質・ペプチド: WD_REPEATS_REGION domain-containing protein
  • タンパク質・ペプチド: Pre-mRNA-splicing factor SYF1
  • タンパク質・ペプチド: TPR_REGION domain-containing protein
  • タンパク質・ペプチド: Pre-mRNA-splicing factor SPF27
  • タンパク質・ペプチド: Cell division cycle 5-like protein
  • タンパク質・ペプチド: Pre-mRNA-splicing factor syf-2
  • タンパク質・ペプチド: Protein BUD31 homolog
  • タンパク質・ペプチド: Pre-mRNA-splicing factor RBM22
  • タンパク質・ペプチド: Spliceosome-associated protein CWC15 homolog
  • タンパク質・ペプチド: GCF C-terminal domain-containing protein
  • タンパク質・ペプチド: Intron-binding protein aquarius
  • タンパク質・ペプチド: Uncharacterized protein T27F2.1
  • タンパク質・ペプチド: Peptidyl-prolyl cis-trans isomerase
  • タンパク質・ペプチド: WD_REPEATS_REGION domain-containing protein
  • タンパク質・ペプチド: Septin and tuftelin-interacting protein 1 homolog
  • タンパク質・ペプチド: WD_REPEATS_REGION domain-containing protein
  • タンパク質・ペプチド: Coiled-coil domain-containing protein 12
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D3
  • タンパク質・ペプチド: Probable small nuclear ribonucleoprotein-associated protein B
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D1
  • タンパク質・ペプチド: Probable small nuclear ribonucleoprotein Sm D2
  • タンパク質・ペプチド: Probable small nuclear ribonucleoprotein E
  • タンパク質・ペプチド: Probable small nuclear ribonucleoprotein F
  • タンパク質・ペプチド: Probable small nuclear ribonucleoprotein G
  • タンパク質・ペプチド: Probable U2 small nuclear ribonucleoprotein A'
  • タンパク質・ペプチド: RRM domain-containing protein
  • タンパク質・ペプチド: Pre-mRNA-processing factor 19
  • タンパク質・ペプチド: Peptidyl-prolyl cis-trans isomerase E
• DNA: Intron lariat RNA
• リガンド: MAGNESIUM ION
• リガンド: INOSITOL HEXAKISPHOSPHATE
• リガンド: GUANOSINE-5'-TRIPHOSPHATE
• リガンド: ZINC ION

超分子 #1: Intron lariat spliceosome

• 超分子: 名称: Intron lariat spliceosome / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#35
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)

分子 #1: U2 snRNA

• 分子: 名称: U2 snRNA / タイプ: rna / ID: 1 ; 詳細: Full sequence: AUCGCUUCUUCGGCUUAUUAGCUAAGAUCAAAGUGUAGUAUCUGUUCUUAUCGUAUUAAC CUACGGUAUACACUCGAAUGAGUGUAAUAAAGGUUAUAUGAUUUUUGGAACCUAGGGAAG ACUCGGGGCUUGCUCCGACUUCCCAAGGGUCGUCCUGGCGUUGCACUGCUGCCGGGCUCGGCCCAGUCCCC; コピー数: 1
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 56.554492 KDa

配列

文字列:

AUCGCUUCUU CGGCUUAUUA GCUAAGAUCA AAGUGUAGUA UCUGUUCUUA UCGUAUUAAC CUACGGUAUA CACUCGAAUG AGUGUAAUA AAGGUUAUAU GAUUUUUGGA ACCUAGGGAA GACUCGGGGC UUGCUCCGAC UUCCCAAGGG (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)U CCCC

GENBANK: GENBANK: X51372.1, GENBANK: X51372.1

分子 #2: U5 snRNA

• 分子: 名称: U5 snRNA / タイプ: rna / ID: 2 / コピー数: 1
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 38.680836 KDa

配列

文字列:

AAACUCUGGU UCCUCUGCAU UUAACCGUGA AAAUCUUUCG CCUUUUACUA AAGAUUUCCG UGCAAAGGAG CAUACAUUGA GUAUUAUAU ACAAUUUUUG GAGUCCCCUC GAAAGAGCGG GA

GENBANK: GENBANK: Z69659.1

分子 #3: U6 snRNA

• 分子: 名称: U6 snRNA / タイプ: rna / ID: 3 / コピー数: 1
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 32.483355 KDa

配列

文字列:

GUUCUUCCGA GAACAUAUAC UAAAAUUGGA ACAAUACAGA GAAGAUUAGC AUGGCCCCUG CGCAAGGAUG ACACGCAAAU UCGUGAAGC GUUCCAAAUU UU

GENBANK: GENBANK: X51387.1

分子 #4: Pre-mRNA-splicing factor 8 homolog

• 分子: 名称: Pre-mRNA-splicing factor 8 homolog / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 272.396156 KDa

配列

文字列:

MANYGGHPQT EPHAIPDSIL EEKSRKWKQL QGKRYSEKKK FGMSDTQKEE MPPEHVRKVI RDHGDMTSRK YRHDKRVYLG ALKYMPHAV LKLLENMPMP WEQIRDVKVL YHITGAITFV NDIPRVIEPV YMAQWGTMWI MMRREKRDRR HFKRMRFPPF D DEEPPLDY ADNILDVEPL EPIQMELDPE EDGAVAEWFY DHKPLATTRF VNGPTYRKWA FSIPQMSTLY RLANQLLTDL VD DNYFYLF DMKSFFTAKA LNVAIPGGPK FEPLVKDLHT DEDWNEFNDI NKVIIRAPIR TEYRIAFPFM YNNLISSLPV QVS WYHTPS VVFIKTEDPD LPAFYYDPLI NPIVLSNLKA TEENLPEGEE EDEWELPEDV RPIFEDVPLY TDNTANGLAL LWAP RPFNL RSGRTRRAVD VPLVKSWYRE HCPAGMPVKV RVSYQKLLKV FVLNALKHRP PKPQKRRYLF RSFKATKFFQ TTTLD WVEA GLQVLRQGYN MLNLLIHRKN LNYLHLDYNF NLKPVKTLTT KERKKSRFGN AFHLCREILR LTKLVVDAHV QYRLNN VDA YQLADGLQYI FAHVGQLTGM YRYKYKLMRQ VRMCKDLKHL IYYRFNTGPV GKGPGCGFWA PGWRVWLFFL RGITPLL ER WLGNLLSRQF EGRHSKGVAK TVTKQRVESH FDLELRAAVM HDILDMMPDG IKQNKARVIL QHLSEAWRCW KANIPWKV P GLPTPVENMI LRYVKAKADW WTNSAHYNRE RVRRGATVDK TVCKKNLGRL TRLYLKSEQE RQHNYLKDGP YISAEEAVA IYTTTVHWLE SRRFSPIPFP PLSYKHDTKL LILALERLKE SYSVKNRLNQ SQREELALIE QAYDNPHEAL SRIKRHMLTQ RAFKEVGIE FMDLYTHLIP VYDIEPLEKV TDAYLDQYLW YEADKRRLFP AWVKPGDTEP PPLLTYKWCQ GLNNLQDVWE T SEGECNVI METKLEKIAE KMDLTLLNRL LRLIVDHNIA DYMTSKNNVL INYKDMNHTN SFGIIRGLQF ASFIVQFYGL VL DLLVLGL RRASEIAGPP QCPNEFLQFQ DVATEIGHPI RLYCRYIDRV WIMFRFSADE ARDLIQRYLT EHPDPNNENI VGY NNKKCW PRDARMRLMK HDVNLGRAVF WDIKNRLPRS ITTVEWENSF VSVYSKDNPN MLFDMSGFEC RILPKCRTAN EEFV HRDGV WNLQNEVTKE RTAQCFLKVD EESLSKFHNR IRQILMSSGS TTFTKIVNKW NTALIGLMTY FREAVVNTQE LLDLL VKCE NKIQTRIKIG LNSKMPSRFP PVVFYTPKEI GGLGMLSMGH VLIPQSDLRW MQQTEAGGVT HFRSGMSHDE DQLIPN LYR YIQPWEAEFV DSVRVWAEYA LKRQEANAQN RRLTLEDLDD SWDRGIPRIN TLFQKDRHTL AYDKGWRVRT EFKAYQI LK QNPFWWTHQR HDGKLWNLNN YRTDMIQALG GVEGILEHTL FRGTYFPTWE GLFWERASGF EESMKFKKLT NAQRSGLN Q IPNRRFTLWW SPTINRANVY VGFQVQLDLT GIFMHGKIPT LKISLIQIFR AHLWQKIHES VVMDLCQVFD QELDALEIQ TVQKETIHPR KSYKMNSSCA DVLLFAQYKW NVSRPSLMAD SKDVMDNTTT QKYWLDVQLR WGDYDSHDVE RYARAKFLDY TTDNMSIYP SPTGVLIAID LAYNLYSAYG NWFPGMKPLI RQAMAKIIKA NPAFYVLRER IRKGLQLYSS EPTEPYLTSQ N YGELFSNQ IIWFVDDTNV YRVTIHKTFE GNLTTKPING AIFIFNPRTG QLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV AA LIRSLPV EEQPRQIIVT RKAMLDPLEV HLLDFPNIVI KGSELMLPFQ AIMKVEKFGD LILKATEPQM VLFNLYDDWL KTI SSYTAF SRVVLIMRGM HINPDKTKVI LKPDKTTITE PHHIWPTLSD DDWIKVELAL KDMILADYGK KNNVNVASLT QSEV RDIIL GMEISAPSQQ RQQIADIEKQ TKEQSQVTAT TTRTVNKHGD EIITATTSNY ETASFASRTE WRVRAISSTN LHLRT QHIY VNSDDVKDTG YTYILPKNIL KKFITISDLR TQIAGFMYGV SPPDNPQVKE IRCIVLVPQT GSHQQVNLPT QLPDHE LLR DFEPLGWMHT QPNELPQLSP QDVTTHAKLL TDNISWDGEK TVMITCSFTP GSVSLTAYKL TPSGYEWGKA NTDKGNN PK GYMPTHYEKV QMLLSDRFLG YFMVPSNGVW NYNFQGQRWS PAMKFDVCLS NPKEYYHEDH RPVHFHNFKA FDDPLGTG S ADREDAFA

UniProtKB: Pre-mRNA-splicing factor 8 homolog

分子 #5: U5 small nuclear ribonucleoprotein 200 kDa helicase

• 分子: 名称: U5 small nuclear ribonucleoprotein 200 kDa helicase / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 244.151594 KDa

配列

文字列:

MADELARIQQ YEYRQNSNLV LSVDYNLTDR RGREEPTGEV LPITDKEMRK MKMGDRAIKG KAPVQDQKKK RKKKDDEKAQ QFGRNVLVD NNELMGAYKP RTQETKQTYE VILSFILDAL GDVPREVLCG AADEVLLTLK NDKFRDKEKK KEVEALLGPL T DDRIAVLI NLSKKISDFS IEEENKPEGD GDIYENEGVN VQFDSDEEED DGGMVNEIKG DSEEESEEEE GVDTDYTATL KG DGHLTED EQKARGILHP RDIDAHWIQR SLAKYFKDPL IAQQKQTEVI GILKNAADDR DAENQLVLLL GFDQFEFIKC LRQ NRLMIL YCTLLRQANE KERLQIEDDM RSRPELHPIL ALLQETDEGS VVQVEKSKRD AEKSKKAATA ANEAISAGQW QAGR KMLDL NDLTFSQGSH LMSNKRCELP DGSYRRQKKS YEEIHVPALK PRPFAEGEKL VSVSELPKWA QPAFDGYKSL NRIQS RLCD SALRSKEHLL LCAPTGAGKT NVALLTMLQE IGNHLAEDGS VKLDEFKIVY IAPMKSLVQE MVGSFSKRLA PFGITV GEM TGDAQMSKEQ FMATQVIVCT PEKYDVVTRK GGERAYNQMV RLLIIDEIHL LHDDRGPVLE SIVVRTIRQM EQNHDEC RL VGLSATLPNY QDVATFLRVK PEHLHFFDNS YRPVPLEQQY IGVTEKKALK RFQAMNEVVY DKIMEHAGKS QVLVFVHS R KETAKTAKAI RDACLEKDTL SAFMREGSAS TEILRTEAEQ AKNLDLKDLL PYGFAIHHAG MNRVDRTLVE DLFADRHIQ VLFSTATLAW GVNLPAHTVI IKGTQIYNPE KGRWTELGAL DIMQMLGRAG RPQYDDRGEG ILITNHSELQ YYLSLMNQQL PVESQMVSR LTDMLNAEVV LGTVSSVSEA TNWLGYTFLF VRMLKNPTLY GITHEQARAD PLLEQRRADL IHTACVLLDK A GLIKYDKR SGIIQATELG RIASHFYCTY ESMQTYNKLL VETCSDIDLF RIFSMSSEFK LLSVRDEEKL ELQKMAEHAP IP IKENLDE ASAKTNVLLQ AYISQLKLEG FALQADMVFV AQSAGRLFRA LFEIVLWRGW AGLAQKVLTL CKMVTQRQWG SLN PLHQFK KIPSEVVRSI DKKNYSFDRL YDLDQHQLGD LIKMPKMGKP LFKFIRQFPK LEMTTLIQPI TRTTMRIELT ITPD FKWDE KVHGSAEGFW IFIEDTDGEK ILHHEFFLLK QKFCSDEHVV KMIVPMFDPM PPLYYVRIVS DRWIGAETVL PISFR HLIL PEKYPPPTEL LDLQPLPISA VTNKEFQTVF AESGFKVFNP IQTQVFRTVF ESNENVIVCA PNGSGKTAIA ELAVLR HFE NTPEAKAVYI TPMEDMATKV YADWKRRLEP AIGHTIVLLT GEQTMDLKLA QRGQLIISTP ERWDNISRRW KQRKSVQ NV KLFIADDLHM IGASNGAVFE VVCSRTRYIS SQLESAVRVV ALSSSLTNAR DLGMWLGCSA SATFNFMPST RPVPLDLE I KSFNLSHNAS RFAAMERPVY QAICRHAGKL EPKPALVFVP VRRQTRPVAV ALLTMALADG APKRFLRLAE HDDTFQALL ADIEDESLRE SVSCGVGFLH EGTAPKDVHI VQQLFESNAI QVCVVPRGMC YQIEMSAYLV VVMDTQFYNG KYHVYEDYPI ADMLHMVGL ANRPILDSDA KCVVMCQTSK RAYYKKFLCD PLPVESHLDH CLHDHFNAEI VTKTIENKQD AIDYLTWTLL Y RRMTQNPN YYNLQGTTHR HLSDALSELV ELTLKDLENS KCIAVKDEMD TVSLNLGMIA SYYYISYQTI ELFSMSLKEK TK TRALIEI ISASSEFGNV PMRHKEDVIL RQLAERLPGQ LKNQKFTDPH VKVNLLIHAH LSRVKLTAEL NKDTELIVLR ACR LVQACV DVLSSNGWLS PAIHAMELSQ MLTQAMYSNE PYLKQLPHCS AALLERAKAK EVTSVFELLE LENDDRSDIL QMEG AELAD VARFCNHYPS IEVATELEND VVTSNDNLML AVSLERDNDI DGLAPPVVAP LFPQKRKEEG WWLVIGDSES NALLT IKRL VINEKSSVQL DFAAPRPGHH KFKLFFISDS YLGADQEFDV AFKVEEPGRS NRKRKHEKEE D

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

分子 #6: Tr-type G domain-containing protein

• 分子: 名称: Tr-type G domain-containing protein / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 110.612859 KDa

配列

文字列:

MDSDLYDEFG NYIGPELDSD DDAGDIDDNG DDEDRSDVDE DDEPDRMEED DAEEIPQNQV VLHEDKKYYA TALEVYGEGV ETLVQEEDA QPLTEPIVKP VSKKKFQAAE RFLPETVYKK EYLADLMDCP HIMRNVAIAG HLHHGKTTFL DCLMEQTHPE F YRAEDADA RFTDILFIEK QRGCSIKSQP VSIVAQDSRS KSYLLNIIDT PGHVNFSDEM TASYRLADGV VVMVDAHEGV MM NTERAIR HAIQERLAVT LCISKIDRLL LELKLPPADA YFKLRLIIDQ VNNILSTFAE EDVPVLSPLN GNVIFSSGRY NVC FSLLSF SNIYAKQHGD SFNSKEFARR LWGDIYFEKK TRKFVKKSPS HDAPRTFVQF ILEPMYKIFS QVVGDVDTCL PDVM AELGI RLSKEEQKMN VRPLIALICK RFFGDFSAFV DLVVQNIKSP LENAKTKIEQ TYLGPADSQL AQEMQKCNAE GPLMV HTTK NYPVDDATQF HVFGRVMSGT LEANTDVRVL GENYSIQDEE DCRRMTVGRL FVRVASYQIE VSRVPAGCWV LIEGID QPI VKTATIAELG YEEDVYIFRP LKFNTRSCVK LAVEPINPSE LPKMLDGLRK VNKSYPLLTT RVEESGEHVL LGTGEFY MD CVMHDMRKVF SEIDIKVADP VVTFNETVIE TSTLKCFAET PNKKNKITMM AEPLEKQLDE DIENEVVQIG WNRRRLGE F FQTKYNWDLL AARSIWAFGP DTTGPNILLD DTLPSEVDKH LLSTVRESLV QGFQWATREG PLCEEPIRQV KFKLLDAAI ATEPLYRGGG QMIPTARRCA YSAFLMATPR LMEPYYTVEV VAPADCVAAV YTVLAKRRGH VTTDAPMPGS PMYTISAYIP VMDSFGFET DLRIHTQGQA FCMSAFHHWQ LVPGDPLDKS IVIKTLDVQP TPHLAREFMI KTRRRKGLSE DVSVNKFFDD P MLLELAKQ QDYTGF

UniProtKB: Tr-type G domain-containing protein

分子 #7: Protein isy-1

• 分子: 名称: Protein isy-1 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 31.326172 KDa

配列

文字列:

MARNAEKAMT ALARWRRMKE EEERGPIARR PHDVKDCRNL SDAERFRREI VRDASKKITA IQNPGLGEFK LRDLNDEVNR LIKLKHAWE QRIRELGGTD YRKYAQKELD AIGRETGNSR GYKYFGAAKD LPGVRELFEK STEGEEQRRH RADLLRNIDA H YFGYLDDE DGRLIPLEKL IEEKNIERIN KEFAEKQAQK QQTASDAAPE NIYKVEEDDD DDLETQESTV IGEDGRPMTI RH VLLPTQQ DIEEMLLEQK KQELMAKYLD

UniProtKB: Protein isy-1

分子 #8: WD_REPEATS_REGION domain-containing protein

• 分子: 名称: WD_REPEATS_REGION domain-containing protein / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 36.865559 KDa

配列

文字列:

MALVTSSGQQ LVSSGFPQQT AQRFSNLMAP TMVLLGHEGE IYTGAFSPDG TCLATSGYDQ KIFFWNVYGE CENFSTIKGH SGAVMDLKF TTDSSSLVSC GTDKSVRVWD METGTCARRF RTHTDFVNAV HPSRRGVTLV ASASDDGTCR VHDMRTKEPV K TYTNRYQQ TAVTFNDSSD QVISGGIDNV LKVWDMRRDE ITYTLTGHRD TITGISLSPS GKFIISNSMD CTVRQWDIRP FV PGQRSVG VFAGHNHNFE KNLLKCSWSP CERFITAGSS DRFLYVWETL SKKIVYKLPG HMGSVNCTDF HPKEPIMLSC GSD KRVFLG EIDMS

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

分子 #9: Pre-mRNA-splicing factor SYF1

• 分子: 名称: Pre-mRNA-splicing factor SYF1 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 99.675094 KDa

配列

文字列:

MADKENATKI EKMPNSETMK GISSEDVPFE EDIIRNPTSV NCWQRYIDHK LQNKSPAKQM FLIYERALAV FERSYKLWYH YLKYRESTI VNKCPTDNSW RALCDTYERC LMRLHKMPRI WICYCEVMIK RGLITETRRV FDRALRSLPV TQHMRIWTLY I GFLTSHDL PETTIRVYRR YLKMNPKARE DYVEYLIERD QIDEAAKELT TLVNQDQNVS EKGRTAHQLW TQLCDLISKN PV KIFSLNV DAIIRQGIYR YTDQVGFLWC SLADYYIRSA EFERARDVYE EAIAKVSTVR DFAQVYDAYA AFEEREVSIM MQE VEQSGD PEEEVDLEWM FQRYQHLMER KNELMNSVLL RQNPHNVGEW LNRVNIYEGN YNKQIETFKE AVKSVNPKIQ VGKV RDLWI GLAKLYEDNG DLDAARKTFE TAVISQFGGV SELANVWCAY AEMEMKHKRA KAALTVMQRA CVVPKPGDYE NMQSV QARV HRSPILWAMY ADYEECCGTV ESCRKVYDKM IELRVASPQM IMNYAMFLEE NEYFELAFQA YEKGIALFKW PGVFDI WNT YLVKFIKRYG GKKLERARDL FEQCLENCPP THAKYIFLLY AKLEEEHGLA RHALSIYNRA CSGVDRADMH SMYNIYI KK VQEMYGIAQC RPIFERAISE LPEDKSRAMS LRYAQLETTV GEIDRARAIY AHAAEISDPK VHVKFWDTWK NFEVAHGN E ATVRDMLRVR RSVEASYNVN VTLTSVQMRV DAERKAQETT TSSNPMDSLD QQQQQPSDGA GSITQVSMNK GNISFVRGA GKTVQQNTTE NPDEIDLDED DDDEEDDGGD ADISVKVVPA QIFGNLKLAE EEEEA

UniProtKB: Pre-mRNA-splicing factor SYF1

分子 #10: TPR_REGION domain-containing protein

• 分子: 名称: TPR_REGION domain-containing protein / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 88.116 KDa

配列

文字列:

MSDDEAAVPG NKPIRLPKKA AKVKNKAPAQ LQITAEQLLR EAKERELELI PPAPKTKITD PDELKEYQRK KRKEFEDGIR KNRMQLANW IKYGKWEESI GEIQRARSVF ERALDVDHRS ISIWLQYAEM EMRCKQINHA RNVFDRAITI MPRAMQFWLK Y SYMEEVIE NIPGARQIFE RWIEWEPPEQ AWQTYINFEL RYKEIDRARS VYQRFLHVHG INVQNWIKYA KFEERNGYIG NA RAAYEKA MEYFGEEDIN ETVLVAFALF EERQKEHERA RGIFKYGLDN LPSNRTEEIF KHYTQHEKKF GERVGIEDVI ISK RKTQYE KMVEENGYNY DAWFDYLRLL ENEETDREEV EDVYERAIAN IPPHSEKRYW RRYIYLWINY ALYEELVAKD FDRA RQVYK ACIDIIPHKT FTFAKVWIMF AHFEIRQLDL NAARKIMGVA IGKCPKDKLF RAYIDLELQL REFDRCRKLY EKFLE SSPE SSQTWIKFAE LETLLGDTDR SRAVFTIAVQ QPALDMPELL WKAYIDFEIA CEEHEKARDL YETLLQRTNH IKVWIS MAE FEQTIGNFEG ARKAFERANQ SLENAEKEER LMLLEAWKEC ETKSGDQEAL KRVETMMPRR VKKRRQIQTE DGVDAGW EE YFDYIFPQDQ AAKGSFKLLE AAARWKRERE EAAARAAQEL DAPIPEGDDD EEKEEAGKDA EEKVREGDSD TDLSESSS S SDSESSSSSS SDSSDSSDDD EDK

UniProtKB: Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats domain-containing protein

分子 #11: Pre-mRNA-splicing factor SPF27

• 分子: 名称: Pre-mRNA-splicing factor SPF27 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 27.679885 KDa

配列

文字列:

MSSKPLALTG GSGSSQLQDD QVLVDALPYL DTEYNEADRQ LAMKLVEHEC KTFRPTKNYL THLPVPDYDA FLTKCMLKEM DRMKKKEEM GKLDMSRCEL PAPSAVKGVD RKLWAKVLRN AKAQNEHLLM RQINLELMDE YAAESYLQRN KVMEDLLTHA E KELRKTKE AVMEVHANRK MAQLKAGEKV KQLEQSWVSM VTNNYRMEME NRQIDSDNRK QIKALKLDPT KLDDKEDQEN

UniProtKB: Pre-mRNA-splicing factor SPF27

分子 #12: Cell division cycle 5-like protein

• 分子: 名称: Cell division cycle 5-like protein / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 85.843469 KDa

配列

文字列:

MVRVIIKGGV WKNTEDEILK AAIMKYGKNQ WSRIASLLHR KSAKQCKARW FEWLDPGIKK TEWSREEDEK LLHLAKLMPT QWRTIAPIV GRTSAQCLER YEHLLDEAQR KAEGLDEEAT ETRKLKPGEI DPTPETKPAR PDPIDMDDDE LEMLSEARAR L ANTQGKKA KRKARERQLS DARRLASLQK RREMRAAGLA FARKFKPKRN QIDYSEEIPF EKHVPAGFHN PSEDRYVVED AN QKAIEDH QKPRGREIEM EMRREDREKL KKRKEQGEAD AVFNIKEKKR SKLVLPEPQI SDRELEQIVK IGHASDSVRQ YID GTATSG LLTDYTESAR ANAVAARTMR TPMLKDTVQL ELENLMALQN TESALKGGLN TPLHESELGK GVLPTPKVAA TPNT VLHAI AATPGTQSQF PGSTPGGFAT PAGSVAATPF RDQMRINEEI AGSALEQKAS LKRALASLPT PKNDFEVVGP DDDEV EGAV EDESNQDEDG WIEDASERAE NKAKRNAENR VRNMKMRSQV IQRSLPKPTK VNEQATRATN SSADDMVKAE MSKLLA WDV DNKPPSVIYS REELDAAADL IKQEAESGPE LNSLMWKVVE QCTSEIILSK DKFTRIAILP REEQMKALND EFQMYRG WM NQRAKRAAKV EKKLRVKLGG YQAIHDKLCK KYQEVTTEIE MANIEKKTFE RLGEHELKAI NKRVGRLQQE VTTQETRE K DLQKMYSKLS NKQWKLSQIE IHDAASTTSA PITY

UniProtKB: Cell division cycle 5-like protein

分子 #13: Pre-mRNA-splicing factor syf-2

• 分子: 名称: Pre-mRNA-splicing factor syf-2 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 27.719021 KDa

配列

文字列:

MSSESQSSSS GPSSSGSKMK DFNQRFRDLH KLRQRARKEN HEQVVEEDRR SKLPKNHEAK KERDQWQVKE LQDRKAAEDK GLDYERVRS LEMSADVTEK LEQKRKRKKN PDQGFTSYED MTLRQHTRLT AALDPDLDSY KKMRECVGGE QFYPTADTLI H GNHYPTTA AMDKLTKDVH GQVKRREQYH RRRLYDPDAP IDYINEKNKK FNKKLDKYYG KYTEDIKDDL ERGTAI

UniProtKB: Pre-mRNA-splicing factor syf-2

分子 #14: Protein BUD31 homolog

• 分子: 名称: Protein BUD31 homolog / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 17.153879 KDa

配列

文字列:

MSLATKLRRV RKSPPEGWDL IEPTLEQFEA KMREAETEPH EGKRKTEINW PIFRIHHQRS RYVYDMYYKK AEISRELYEF CLTAKFADA ALIAKWKKQG YENLCCVKCV NTRDSNFGTA CICRVPKSKL DAERVIECVH CGCHGCSG

UniProtKB: Protein BUD31 homolog

分子 #15: Pre-mRNA-splicing factor RBM22

• 分子: 名称: Pre-mRNA-splicing factor RBM22 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 45.90275 KDa

配列

文字列:

MSMSKSSYSQ YNRKNWEDSD FPILCETCLG NNPYMRMMKD KYGRECKICE RPFTTFRWQP GKGARYKNTE LCQTCAKVKN VCQTCMFDL EYGLPVQVRD HELQIADNIP KQGANRDFFL QNVERTLGQG DGTQPIAQIA NNMDQAAHDR LRRMGRTQPY Y KRNAPHIC SFFVKGECKR GEECPYRHEK PTDPDDPLSR QNIRDRYYGT NDPVAEKILN RAAAAPTLSP PADTTITTLY IG NLGPSGA QQVTEKDLND FFYQYGDIRC LRVLTEKGCA FIEFTTREAA ERAAERSFNK TFIKGKRLTI RWGEPQAKRA ADN SNYVTP VPSVPILPVP DGLAPSTSSQ QRFTGSMPRP PAPPTFAAPR SLVVPNVRPV KAGESSGASS SSSIYYPSQD PTRL GAKGD VIE

UniProtKB: Pre-mRNA-splicing factor RBM22

分子 #16: Spliceosome-associated protein CWC15 homolog

• 分子: 名称: Spliceosome-associated protein CWC15 homolog / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 26.154846 KDa

配列

文字列:

MTTAHRPTFH PARGGTARGE GDLSKLSNQY SSKDMPSHTK MKYRQTGQET EADLRKKDLR RELEDKERNA IREKRARDSA SSSSSHSKR QRMDQIAAES AASVDADEAV DELNSSDDDD SDEDDTAALM AELEKIKKER AEEKAARDEE IKEKEEKQRM E NILAGNPL LNDTPAGSST SGGDFTVKRR WDDDVVFKNC AKGVEERKKE VTFINDAIRS EFHKKFMDKY IK

UniProtKB: Spliceosome-associated protein CWC15 homolog

分子 #17: GCF C-terminal domain-containing protein

• 分子: 名称: GCF C-terminal domain-containing protein / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 94.244891 KDa

配列

文字列:

MFRKPKAKGA IRQRKSDGWD EPDAENQQVV SAIEVKQPAV PRPAMSFDAD EGADSTFKLK KDKKKVEELK RQHKLEEEAE KLYKEEKIR KEALDKIVKK EKLSKEDKKT KNERHKYLDK YRDKSAKHIS NSESYEYEEN LDIDAEAISS VSNKFNSAFE G IPDSRAVF EAKKRRERAR REGNQDGYIP LDDTQKLKSK SERNRLIRED ENDDSDEECT NKFYSARELL RTEEDRRREE QE GFLEREN GDIDEAERIK GDDDSENEEW EKQQIRKAVS RREIGQLRTE KRNTSKLFGH TVPVEDDTAM DMDIDLDMDV QVI GKPEFT GPSNTGGVVK IEDILAKLKL RIQERDEALN FRKEEKRKLE QNIEENKSMI AKIEMELPNQ STKYTMYQEL RVYS RSLLE CLNEKVGEIN SIIDKKRDCG KSRTSRLSVR RRQDMRDQHA ECMQGRNARM GEAAGRAAER DARRGRRRRE REFTL ARIN HEEGLSTDDE EPTPQSMNDQ KICDEVEAVA SVLFADALDE YSDLRKVFGR MTDWLAVDPK SFQDAYVYLC IPKLSS PYV RLQILRADFL RKETILTSMQ WFHIAMLAGS ENAEIDQSHE ILVELAPAIV EKVVIPFLID TVKEEWDPMS LRQTRHL TT FCSLFEKLPN LTEKSKQFNA FLNAIRERIC DCISEDLFMP IFMPNALEQP ICRQFHDRQF WTCIKLIKSI NALSPLIS I AARFELVVEK CVNSQCVMAL RTGSKNDVTA ERKVRGLLAE LDDSLLKMGG RTSFRQLIGT LELIAEEQSK AGRSFHKEI RKFLEKLER

UniProtKB: GCF C-terminal domain-containing protein

分子 #18: Intron-binding protein aquarius

• 分子: 名称: Intron-binding protein aquarius / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 170.397688 KDa

配列

文字列:

MVTKRHQEAV VTRGAIENDT ISAVAAKFWA PFTAETHENF DAKLIDTIYD NEMLKTSFNS RKIMMLEFSQ YLEAYLWPNY VPEKASKAW NMSIVVMINE KFRERNLDSW NCFTKKSEHF PHFFKSILQL SLQEEGLASS EHCALLTFLV NAFGSVETPI V HKETRKLV SIEIWAGLLD SQREDLFKKQ KKLKKIWENV RQKMTAAAAD NNEFERTYLW NLIEKFKRVL NSLEPNEAQE SE EGEVRDP IDSIKYCERF IELLIDLESI LQTRRFFNSV LHSSHILTHC LLSSLISTDA GSLFFQLVQL LKFYARFEID DLS GRQLTH KEVSEQHYQS VTRLQKAAFR LFNETMKEFY VLNVSGVDTR RALQKQFGDM NHAEVYRFAE YLHLVPAFGE DPNH QTSLL HLYPHQHLVE TITLHCERRP NQLTQLNEKP LFPTEKVIWD ENIIPYENYT GDGVLALDKL NLQFLTLHDY LLRNF NLFQ LESTYEIRQD LEDVLFRMKP FQHESRNETV FSGWARMALQ IDHFQISEVA KPLVGEKSPA VVRGVVTVNI GRRQDI RQE WENLRKHDVC FLVACRSRKS ASGLKFDVRR PFSEQIEVLS VRGCDVEGML DQDGHLLEEF TAWEKKAKIP GDLRKFR LL LDPNQYRIDM EQGTKDDIYD TFNLIVRRDS KTNNFKAVLQ TIRDLLNTEC VVPDWLTDVI LGYGEPDSAH YSKLSSAV P ELDFNDTFLS FAHVKESFPG YKIELADGFD EKEAVPPFKL EFKELERRQD VEIKPGELRT ILVTPLTRKK VTPYSYDPR KNQVKFTPSQ VEAIKSGMQP GLTMVVGPPG TGKTDVAVQI ISNIYHNWPN QRTLIVTHSN QALNQLFEKI IALDVDERHL LRMGHGEEA LETEKDFSRY GRVNYVLKER LQLLNCVEKL AKALKIVGDV AYTCENAGYF FRFSVCRVWE EFLAKVTSKG C NKLAEGII SEIFPFTGFF KDIPDLFSGN NSADLKVAHS CWRHIEQIFE KLDEFRAFEL LRNGRDRTEY LLVKEAKIIA MT CTHAALR RNELVKLGFR YDNIVMEEAA QILEVETFIP LLLQNPQDGH NRLKRWIMIG DHHQLPPVVQ NQAFQKYSNM EQS LFARLV RLSVPNVQLD RQGRARAQIA ELYQWRYNGL GNLPHVDGLP QFQNANAGFA FPFQFIDIPD FNGHGETQPS PHFY QNLGE AEYACALYTY MRILGYPAEK ISILTTYNGQ AQLIRDVFQR RCDTNPLIGM PAKVSTVDKY QGQQNDFIIL SLVKT RNIG HIRDVRRLVV ALSRARLGLY VLGRSKVFMD CLELTPAMRI FAKYPRKLVI LPFEAHPTIR KWNERSKDGE PMEIQD TLH MTHFVHEFYM SNLPAMRDAY EQAMNEYMES QRLLNPPIDE TQMDVETEHE KKHREAMERK KKQEMDDKKE ADIHFED MD HEMQEPAATA APAPGAPAVE EPPPK

UniProtKB: Intron-binding protein aquarius

分子 #19: Uncharacterized protein T27F2.1

• 分子: 名称: Uncharacterized protein T27F2.1 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 60.303516 KDa

配列

文字列:

MSMKLRDILP APVAADEAAS QIRRDPWFGG RDNEPSAALV SKEPPPYGKR TSFRPRGPED FGDGGAFPEI HVAQFPLGLG LGDMRGKPE NTLALQYGTD GKLQHDAIAR IGHVKDKVVY SKLNDMKAKT WNEDDDDIQK PDDDAVIDAT EKTRMALEKI V NSKVASAL PVRHADKLAP AQYIRYTPSQ QNGAAGSQQR IIRMVEEQKD PMEPPKFKIN QKIPRAPPSP PAPVMHSPPR KM TAKDQND WKIPPCISNW KNPKGFTVGL DKRLAADGRG LQQTHINENF AKLADALYIA DRKAREEVET RAQLERRVAQ NKK SEQEAK MAEAAAKARQ ERSAMRRKDD EDDEQVKVRE EIRRDRLDDI RKERNIARSR PDKADKLRKE RERDISEKIV LGLP DTNQK RTGEPQFDQR LFDKTQGLDS GAMDDDTYNP YDAAWRGGDS VQQHVYRPSK NLDNDVYGGD LDKIIEQKNR FVADK GFSG AEGSSRGSGP VQFEKDQDVF GLSSLFEHTK EKKRGGDGGD SRGESKRSRR D

UniProtKB: Uncharacterized protein T27F2.1

分子 #20: Peptidyl-prolyl cis-trans isomerase

• 分子: 名称: Peptidyl-prolyl cis-trans isomerase / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO / EC番号: peptidylprolyl isomerase
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 18.547002 KDa

配列

文字列:

MPAPINDQAP YVILDTTMGK IALELYWNHA PRTCQNFSQL AKRNYYNGTI FHRIIADFMI QGGDPTGTGR GGASIYGDKF SDEIDERLK HTGAGILSMA NAGPNTNGSQ FFITLAPTQH LDGKHTIFGR VAAGMKVIAN MGRVDTDNHD RPKIEIRILK A YPSESSVL S

UniProtKB: Peptidyl-prolyl cis-trans isomerase

分子 #21: WD_REPEATS_REGION domain-containing protein

• 分子: 名称: WD_REPEATS_REGION domain-containing protein / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 54.766215 KDa

配列

文字列:

MSASVSDPYE QMPAAPTDDD LEDKPEADKK ALLNQVFKSL KRAQDLFYHD YAQPPPMPEE NDSLIRSMKR KHEYGNVIKK VEEMKVRRE NEMLALPTSQ PMHGTGSVIA SAGTPLAITD GSGKLVNQQQ GSAKSGTLLP LVPLGNSSKG EDNTTRSLLP S KAPMMMKP KWHAPWKLYR VASGHTGWVR AVDVEPGNQW FASGGADRII KIWDLASGQL KLSLTGHISS VRAVKVSPRH PF LFSGGED KQVKCWDLEY NKVIRHYHGH LSAVQALSVH PSLDVLVTCA RDSTARVWDM RTKAQVHCFA GHTNTVADVV CQS VDPQVI TASHDATVRL WDLAAGRSMC TLTHHKKSVR ALTIHPRLNM FASASPDNIK QWKLPKGEFM QNLSGHNAII NTLS SNDDG VVVSGADNGS LCFWDWRSGF CFQKIQTKPQ PGSIESEAGI YASCFDKTGL RLITAEADKT IKMYKEDDEA TEESH PIVW RPEIVKKKAY

UniProtKB: Pleiotropic regulator 1

分子 #22: Septin and tuftelin-interacting protein 1 homolog

• 分子: 名称: Septin and tuftelin-interacting protein 1 homolog / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 94.421539 KDa

配列

文字列:

MEDDDGRESF EINDMDLEYA MNPGGRRRFQ NKDQATYGVF APDSDDDDDE QGTSRGPYKK RSKISAPMSF VSGGIQQGNK IDKDDPASL NLNLGGEKKP KEDDEGSIQI DFDKRTKKAP KQNGAQVFAG MRSSANHGAA DINQFGSWMR GDGNSNKIMK M MQAMGYKP GEGLGAQGQG IVEPVQAQLR KGRGAVGAYG KESTATGPKF GESAADAQKR MAQEGTSSRP TNDDQEKSGL KI KGSWKKS QTVKTKYRTI EDVMEEGMSA SRPASHQQSQ QYSNIKVIDM TGKQQKIYSG YDSFSMKTRS EYDTVDDEER TVF DVPELI HNLNLLVDLT EEGIRRSNQQ LISLKDQTTA LEYDLQQVQK SLGTEEQEAQ HIKDVYELID GFSSNRSPSM EECQ ELFRR LRSEFPHEYE LYSLETVAIP TVLPLIQKYF VAWKPLEDKN YGCELISTWR DILDDSKNGR KMTFGHNKTK GDEIR AYDR IIWEGILPSI RRACLQWDPS TQMHEMIELV EQWIPLLSAW ITENILEQLV VPKIAERVNQ WDPMTDEIPI HEWLVP WLV LLGDRIQTVM PPIRQKLSKA LKLWDPMDRS ALETLRPWQN VWSAATFSAF IAQNIVPKLG VALDTMELNP TMNPEYP EW TACMEWLEFT HPDAIANIVT KYFFPRFYNC LCLWLDSPGV DYNEVKRWYG SWKARIPQVL VNYPTVNENL RRSMIAIG R SLQGEKVGGL QATPIAPMAP PPPMAPHFTQ AAPVQKLSLK EIIEYTAGKN GFTYHPQKDR YKDGRQVFWF GALSIYLDS EMVYVMDPIE FVWRPSGLNE LIQMAQGAQG

UniProtKB: Septin and tuftelin-interacting protein 1 homolog

分子 #23: WD_REPEATS_REGION domain-containing protein

• 分子: 名称: WD_REPEATS_REGION domain-containing protein / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 65.385664 KDa

配列

文字列:

MDALQAYGGS DSEHSDDDAS MDQVAKGKSS TLLERAIVTA PDVESKSAIR QVAIVDPKTK EIKSNPKFDQ LFKPESGPVN HFKSEQQRS QKNTLTGFVE PAHLNEFHFN RQIRSFDTLG YAQNPTAESG TTHFVGDVKK AEAEKGVSLF ESKKTGGEKR K RVRNDDSA DIDGYTGPWS RFIDEKTVAK PTPELQKQMD EIVKKRQEKS RRFKKEKEDS EQMAEESSTL HLKEAEDYQG RS FLVPPSF TGVNLREDYV PERCFVPKKL VHTYRGHNKG VNFLQWFPKS AHLFLSCSMD TKIKLWEVYD RQRVVRTYAG HKL PVREVA FNNEGTEFLS ASFDRYVKLW DTETGQVKQR FHTGHVPYCL KYHPDDDKNH MFLVGMQNKK IIQWDSRSGE IVQE YDRHL QAVNSITFFD KNRRFASTSD DKSVRIWEWE IPVDTKLIQN VGLHAIPTMT KSPNDKWVVG QCMDNRIVLF QLVDD KLRF SKKKAFRGHN AAGYACNIDF SPDQSFLISG DADGKLFIWD WRTHKIVGKW KAHDSTCIAA LWHPHEKSRM ITAGWD GLI KMWN

UniProtKB: Pre-mRNA-processing factor 17

分子 #24: Coiled-coil domain-containing protein 12

• 分子: 名称: Coiled-coil domain-containing protein 12 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 19.138486 KDa

配列

文字列:

MTDKNNSDSD EDIESLTNHE TSLEAAAKAR KRRLLAMKSK IHGIEMQEED YDEGETSTKK SREVGREFRN HKPDDAVGTQ NVDMDLDIV QREITEHLKD VLHEKAIDSV DLAMLAPKKI DWDLKRDIES KLQKLERRTQ KAVATIIRQR LAEGKGDLAA T VNAAAAQN L

UniProtKB: Coiled-coil domain-containing protein 12

分子 #25: Small nuclear ribonucleoprotein Sm D3

• 分子: 名称: Small nuclear ribonucleoprotein Sm D3 / タイプ: protein_or_peptide / ID: 25 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 14.836212 KDa

配列

文字列:

MTSVGVPIKI LHEAEGHMVT LETVTGEVYR GKLSEAEDNM NCQLAETVVT FRDGRSHQLD NVFIRGNKIR FMILPDMLKN APMFKNIGR AQKGAIGMGL GGLDQRGRGR GTAFRRPMGR GGPRGMSRPG GAPTFRG

UniProtKB: Small nuclear ribonucleoprotein Sm D3

分子 #26: Probable small nuclear ribonucleoprotein-associated protein B

• 分子: 名称: Probable small nuclear ribonucleoprotein-associated protein B; タイプ: protein_or_peptide / ID: 26 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 16.768627 KDa

配列

文字列:

MTISKNNKMM AHLNYRMKII LQDGRTFIGF FKAFDKHMNI LLAECEEHRQ IKPKAGKKTD GEEKRILGLV LVRGEHIVSM TVDGPPPRD DDSVRLAKAG GAGGVGQAKP GGRGMPAMPG MPGMPPGGAP GGLSGAMRGH GGPGMAAMQP GYGGPPGGRP F

UniProtKB: Probable small nuclear ribonucleoprotein-associated protein B

分子 #27: Small nuclear ribonucleoprotein Sm D1

• 分子: 名称: Small nuclear ribonucleoprotein Sm D1 / タイプ: protein_or_peptide / ID: 27 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 13.72407 KDa

配列

文字列:

MKLVRFLMKL SHETVNIELK NGTQVSGTIM GVDVAMNTHL RAVSMTVKNK EPVKLDTLSI RGNNIRYIIL PDPLALDTLL IDDEPRKKA RAARAGASRG RGGRGGMRGG RGGRGRGRGG PRGAGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

分子 #28: Probable small nuclear ribonucleoprotein Sm D2

• 分子: 名称: Probable small nuclear ribonucleoprotein Sm D2 / タイプ: protein_or_peptide / ID: 28 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 13.291529 KDa

配列

文字列:

MSAQAKPRSE MTAEELAAKE DEEFNVGPLS ILTNSVKNNH QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKT GKGKKKAKS VAKDRFISKM FLRGDSVILV VKNPLAQAE

UniProtKB: Probable small nuclear ribonucleoprotein Sm D2

分子 #29: Probable small nuclear ribonucleoprotein E

• 分子: 名称: Probable small nuclear ribonucleoprotein E / タイプ: protein_or_peptide / ID: 29 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 10.625318 KDa

配列

文字列:

MSTRKLNKVM VQPVNLIFRY LQNRTRVQIW LYEDVTHRLE GYIIGFDEFM NVVFDEAEEV NMKTKGRNKI GRILLKGDNI TLIHAAQQE A

UniProtKB: Probable small nuclear ribonucleoprotein E

分子 #30: Probable small nuclear ribonucleoprotein F

• 分子: 名称: Probable small nuclear ribonucleoprotein F / タイプ: protein_or_peptide / ID: 30 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 9.256534 KDa

配列

文字列:

MSAVQPVNPK PFLNSLTGKF VVCKLKWGME YKGVLVAVDS YMNLQLAHAE EYIDGNSQGN LGEILIRCNN VLYVGGVDGE NETSA

UniProtKB: Probable small nuclear ribonucleoprotein F

分子 #31: Probable small nuclear ribonucleoprotein G

• 分子: 名称: Probable small nuclear ribonucleoprotein G / タイプ: protein_or_peptide / ID: 31 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 8.756209 KDa

配列

文字列:

MSKTHPPELK KYMDKEMDLK LNGNRRVSGI LRGFDPFMNM VIDEAVEYQK DGGSVNLGMT VIRGNSVVIM EPKERIS

UniProtKB: Probable small nuclear ribonucleoprotein G

分子 #32: Probable U2 small nuclear ribonucleoprotein A'

• 分子: 名称: Probable U2 small nuclear ribonucleoprotein A' / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 28.9059 KDa

配列

文字列:

MVRLTTELFA ERPQFVNSVN MREINLRGQK IPVIENMGVT RDQFDVIDLT DNDIRKLDNF PTFSRLNTLY LHNNRINYIA PDIATKLPN LKTLALTNNN ICELGDIEPL AECKKLEYVT FIGNPITHKD NYRMYMIYKL PTVRVIDFNR VRLTEREAAK K MFKGKSGK KARDAIQKSV HTEDPSEIEP NENSSGGGAR LTDEDREKIK EAIKNAKSLS EVNYLQSILA SGKVPEKGWN RQ MDQNGAD GEAMES

UniProtKB: Probable U2 small nuclear ribonucleoprotein A'

分子 #33: RRM domain-containing protein

• 分子: 名称: RRM domain-containing protein / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 24.881344 KDa

配列

文字列:

MADINPNHTI YVNNLNEKVK KDELKRSLHM VFTQFGEIIQ LMSFRKEKMR GQAHIVFKEV SSASNALRAL QGFPFYGKPM RIQYAREDS DVISRAKGTF VEKRQKSTKI AKKPYEKPAK NGKSAAEPTQ KEPQETDGPG LPNNILFCSN IPEGTEPEQI Q TIFSQFPG LREVRWMPNT KDFAFIEYES EDLSEPARQA LDNFRITPTQ QITVKFASK

UniProtKB: RRM domain-containing protein

分子 #34: Pre-mRNA-processing factor 19

• 分子: 名称: Pre-mRNA-processing factor 19 / タイプ: protein_or_peptide / ID: 34 / コピー数: 4 / 光学異性体: LEVO / EC番号: RING-type E3 ubiquitin transferase
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 53.272633 KDa

配列

文字列:

MSFVCGISGE LTEDPVVSQV SGHIFDRRLI VKFIAENGTD PISHGELSED QLVSLKSGGT GSAPRNVSGT SIPSLLKMLQ DEWDTVMLN SFSLRQQLQI ARQELSHSLY QHDAACRVIS RLSKELTAAR EALSTLKPHT SAKVDDDVSI DESEDQQGLS E AILAKLEE KSKSLTAERK QRGKNLPEGL AKTEELAELK QTASHTGIHS TGTPGITALD IKGNLSLTGG IDKTVVLYDY EK EQVMQTF KGHNKKINAV VLHPDNITAI SASADSHIRV WSATDSSSKA IIDVHQAPVT DISLNASGDY ILSASDDSYW AFS DIRSGK SLCKVSVEPG SQIAVHSIEF HPDGLIFGTG AADAVVKIWD LKNQTVAAAF PGHTAAVRSI AFSENGYYLA TGSE DGEVK LWDLRKLKNL KTFANEEKQP INSLSFDMTG TFLGIGGQKV QVLHVKSWSE VVSLSDHSGP VTGVRFGENA RSLVT CSLD KSLRVFSF

UniProtKB: Pre-mRNA-processing factor 19

分子 #35: Peptidyl-prolyl cis-trans isomerase E

• 分子: 名称: Peptidyl-prolyl cis-trans isomerase E / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO / EC番号: peptidylprolyl isomerase
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 36.469277 KDa

配列

文字列:

MNTNFPHNRK RTLYVGGFTE DVTEKVLMAA FIPFGDVVAI SIPMDYESGK HRGFGFVEFD MAEDAAMAID NMNESELFGK TIRVNFARP PKATERSQKP VWADDEWLKK YGRGGEAAAE EDGDAEKAAT SSSSASTKLP RVYLGVKIGI RYIGRIVIEL R TDVTPKTA ENFRCLCTGE RGFGYEGSIF HRIIPKFMLQ GGDFTKGDGT GGKSIYGTKF DDENFTLRHT MPGTVSMANC GA NTNGSQF FICTEKTDWL DGKHVVFGHV VEGMNIVRQV EQQGTPSGKP QMVVKIVESG EIEPEKRIAA EKLAQKAVVP GAE IQEPLP QAMET

UniProtKB: Peptidyl-prolyl cis-trans isomerase E

分子 #36: Intron lariat RNA

• 分子: 名称: Intron lariat RNA / タイプ: dna / ID: 36 / コピー数: 1 / 分類: DNA
• 由来(天然): 生物種: Caenorhabditis elegans (センチュウ)
• 分子量: 理論値: 3.680845 KDa

配列

文字列:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)

分子 #37: MAGNESIUM ION

• 分子: 名称: MAGNESIUM ION / タイプ: ligand / ID: 37 / コピー数: 7 / 式: MG
• 分子量: 理論値: 24.305 Da

分子 #38: INOSITOL HEXAKISPHOSPHATE

• 分子: 名称: INOSITOL HEXAKISPHOSPHATE / タイプ: ligand / ID: 38 / コピー数: 2 / 式: IHP
• 分子量: 理論値: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

分子 #39: GUANOSINE-5'-TRIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 39 / コピー数: 1 / 式: GTP
• 分子量: 理論値: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子*YM

分子 #40: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 40 / コピー数: 6 / 式: ZN
• 分子量: 理論値: 65.409 Da

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 緩衝液: pH: 7.9
• 凍結: 凍結剤: ETHANE
• 詳細: Crosslinked with glutaraledhyde

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 撮影: フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k); 平均電子線量: 60.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2.0 µm; 最小 デフォーカス（公称値）: 0.7000000000000001 µm
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

初期モデル

モデルのタイプ: PDB ENTRY
PDBモデル - PDB ID:

6id1

• 最終 再構成: 解像度のタイプ: BY AUTHOR / 解像度: 2.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 879523
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD

原子モデル構築 1

• 初期モデル: Chain - Source name: AlphaFold / Chain - Initial model type: in silico model
• 精密化: プロトコル: AB INITIO MODEL

得られたモデル

PDB-8ro0:
Structure of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')