+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-6428 | |||||||||
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タイトル | Cryo-EM structure of MAVS CARD C1 filament | |||||||||
マップデータ | Cryo-EM map of MAVS CARD filament | |||||||||
試料 |
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キーワード | Innate immunity / helical filament | |||||||||
機能・相同性 | 機能・相同性情報 positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway ...positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway / peroxisomal membrane / TRAF6 mediated IRF7 activation / negative regulation of type I interferon-mediated signaling pathway / positive regulation of NLRP3 inflammasome complex assembly / cytoplasmic pattern recognition receptor signaling pathway / negative regulation of viral genome replication / type I interferon-mediated signaling pathway / cellular response to exogenous dsRNA / TRAF6 mediated NF-kB activation / antiviral innate immune response / positive regulation of type I interferon production / positive regulation of interferon-alpha production / signaling adaptor activity / ubiquitin ligase complex / positive regulation of defense response to virus by host / activation of innate immune response / positive regulation of interferon-beta production / molecular condensate scaffold activity / positive regulation of interleukin-8 production / Negative regulators of DDX58/IFIH1 signaling / mitochondrial membrane / DDX58/IFIH1-mediated induction of interferon-alpha/beta / PKR-mediated signaling / Evasion by RSV of host interferon responses / positive regulation of DNA-binding transcription factor activity / positive regulation of protein import into nucleus / SARS-CoV-1 activates/modulates innate immune responses / positive regulation of interleukin-6 production / positive regulation of tumor necrosis factor production / Ovarian tumor domain proteases / TRAF3-dependent IRF activation pathway / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / mitochondrial outer membrane / molecular adaptor activity / defense response to bacterium / positive regulation of protein phosphorylation / innate immune response / protein kinase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / identical protein binding 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 4.2 Å | |||||||||
データ登録者 | Xu H / He X / Zheng H / Huang LJ / Hou F / Yu Z / de la Cruz MJ / Borkowski B / Zhang X / Chen ZJ / Jiang Q-X | |||||||||
引用 | ジャーナル: Elife / 年: 2014 タイトル: Structural basis for the prion-like MAVS filaments in antiviral innate immunity. 著者: Hui Xu / Xiaojing He / Hui Zheng / Lily J Huang / Fajian Hou / Zhiheng Yu / Michael Jason de la Cruz / Brian Borkowski / Xuewu Zhang / Zhijian J Chen / Qiu-Xing Jiang / 要旨: Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling ...Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling cascades, but the underlying structural mechanism is unknown. Here we report cryo-electron microscopic structures of the helical filaments formed by both the N-terminal caspase activation and recruitment domain (CARD) of MAVS and a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain. Both structures are left-handed three-stranded helical filaments, revealing specific interfaces between individual CARD subunits that are dictated by electrostatic interactions between neighboring strands and hydrophobic interactions within each strand. Point mutations at multiple locations of these two interfaces impaired filament formation and antiviral signaling. Super-resolution imaging of virus-infected cells revealed rod-shaped MAVS clusters on mitochondria. These results elucidate the structural mechanism of MAVS polymerization, and explain how an α-helical domain uses distinct chemical interactions to form self-perpetuating filaments. DOI: http://dx.doi.org/10.7554/eLife.01489.001. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_6428.map.gz | 3.4 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-6428-v30.xml emd-6428.xml | 11.8 KB 11.8 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_6428.tif | 4.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-6428 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6428 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_6428_validation.pdf.gz | 80.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_6428_full_validation.pdf.gz | 79.3 KB | 表示 | |
XML形式データ | emd_6428_validation.xml.gz | 494 B | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-6428 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-6428 | HTTPS FTP |
-関連構造データ
類似構造データ | |
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電子顕微鏡画像生データ | EMPIAR-10031 (タイトル: New movie data for MAVS CARD C1 filaments / Data size: 512.1 Data #1: Falcon 2 Direct Electron Detector movie data [micrographs - multiframe]) |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_6428.map.gz / 形式: CCP4 / 大きさ: 15.3 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM map of MAVS CARD filament | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.05 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : MAVS CARD filament
全体 | 名称: MAVS CARD filament |
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要素 |
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-超分子 #1000: MAVS CARD filament
超分子 | 名称: MAVS CARD filament / タイプ: sample / ID: 1000 / 集合状態: filament / Number unique components: 1 |
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-分子 #1: Mitochondrial antiviral-signaling protein (MAVS), CARD
分子 | 名称: Mitochondrial antiviral-signaling protein (MAVS), CARD タイプ: protein_or_peptide / ID: 1 / Name.synonym: IPS1, KIAA1271, VISA / 集合状態: filament / 組換発現: Yes |
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由来(天然) | 生物種: Homo sapiens (ヒト) / 別称: Human |
分子量 | 理論値: 13 KDa |
組換発現 | 生物種: Homo sapiens (ヒト) / 組換細胞: HEK293T / 組換プラスミド: pcDNA3 |
配列 | UniProtKB: Mitochondrial antiviral-signaling protein |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | らせん対称体再構成法 |
試料の集合状態 | filament |
-試料調製
濃度 | 0.1 mg/mL |
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緩衝液 | pH: 7.5 / 詳細: 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT |
グリッド | 詳細: Quantifoil R2/2 grids coated with thin carbon on top |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: FEI VITROBOT MARK III |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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日付 | 2014年11月5日 |
撮影 | カテゴリ: CCD フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 実像数: 433 / 平均電子線量: 35 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 0.01 mm / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 2.5 µm / 倍率(公称値): 59000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
詳細 | Relion, IHRSR |
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最終 再構成 | 想定した対称性 - らせんパラメータ - Δz: 5.06 Å 想定した対称性 - らせんパラメータ - ΔΦ: 101.21 ° 想定した対称性 - らせんパラメータ - 軸対称性: C1 (非対称) 解像度のタイプ: BY AUTHOR / 解像度: 4.2 Å / 解像度の算出法: OTHER / ソフトウェア - 名称: Relion, IHRSR / 詳細: Relion-based IHRSR |
CTF補正 | 詳細: Each particle |
-原子モデル構築 1
初期モデル | PDB ID: Chain - Chain ID: A |
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ソフトウェア | 名称: Chimera |
詳細 | One MAVS CARD molecule was docked into the EM map by rigid body docking. Symmetric copies were generated in Chimera. |
精密化 | 空間: REAL / プロトコル: RIGID BODY FIT |