[English] 日本語
Yorodumi- EMDB-24625: Archaeal DNA ligase and heterotrimeric PCNA in complex with end-j... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-24625 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Archaeal DNA ligase and heterotrimeric PCNA in complex with end-joined DNA | |||||||||
Map data | Sharpened map | |||||||||
Sample |
| |||||||||
Keywords | DNA ligase / PCNA / cryo-EM / LIGASE-DNA complex | |||||||||
Function / homology | Function and homology information base-excision repair, DNA ligation / DNA ligase (ATP) / DNA ligase (ATP) activity / DNA ligation / lagging strand elongation / DNA polymerase processivity factor activity / DNA biosynthetic process / leading strand elongation / regulation of DNA replication / mismatch repair ...base-excision repair, DNA ligation / DNA ligase (ATP) / DNA ligase (ATP) activity / DNA ligation / lagging strand elongation / DNA polymerase processivity factor activity / DNA biosynthetic process / leading strand elongation / regulation of DNA replication / mismatch repair / translesion synthesis / double-strand break repair / DNA recombination / cell cycle / cell division / DNA binding / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Saccharolobus solfataricus (archaea) / Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.2 Å | |||||||||
Authors | Sverzhinsky A / Pascal JM | |||||||||
Funding support | United States, 2 items
| |||||||||
Citation | Journal: Structure / Year: 2022 Title: Cryo-EM structures and biochemical insights into heterotrimeric PCNA regulation of DNA ligase. Authors: Aleksandr Sverzhinsky / Alan E Tomkinson / John M Pascal / Abstract: DNA ligases act in the final step of many DNA repair pathways and are commonly regulated by the DNA sliding clamp proliferating cell nuclear antigen (PCNA), but there are limited insights into the ...DNA ligases act in the final step of many DNA repair pathways and are commonly regulated by the DNA sliding clamp proliferating cell nuclear antigen (PCNA), but there are limited insights into the physical basis for this regulation. Here, we use single-particle cryoelectron microscopy (cryo-EM) to analyze an archaeal DNA ligase and heterotrimeric PCNA in complex with a single-strand DNA break. The cryo-EM structures highlight a continuous DNA-binding surface formed between DNA ligase and PCNA that supports the distorted conformation of the DNA break undergoing repair and contributes to PCNA stimulation of DNA ligation. DNA ligase is conformationally flexible within the complex, with its domains fully ordered only when encircling the repaired DNA to form a stacked ring structure with PCNA. The structures highlight DNA ligase structural transitions while docked on PCNA, changes in DNA conformation during ligation, and the potential for DNA ligase domains to regulate PCNA accessibility to other repair factors. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_24625.map.gz | 22 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-24625-v30.xml emd-24625.xml | 22.6 KB 22.6 KB | Display Display | EMDB header |
Images | emd_24625.png | 135 KB | ||
Filedesc metadata | emd-24625.cif.gz | 7.1 KB | ||
Others | emd_24625_additional_1.map.gz | 22 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-24625 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-24625 | HTTPS FTP |
-Validation report
Summary document | emd_24625_validation.pdf.gz | 465.8 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_24625_full_validation.pdf.gz | 465.4 KB | Display | |
Data in XML | emd_24625_validation.xml.gz | 4.9 KB | Display | |
Data in CIF | emd_24625_validation.cif.gz | 5.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24625 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24625 | HTTPS FTP |
-Related structure data
Related structure data | 7rpxMC 7rpoC 7rpwC C: citing same article (ref.) M: atomic model generated by this map |
---|---|
Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_24625.map.gz / Format: CCP4 / Size: 24.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Sharpened map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.5 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Additional map: Unsharpened map
File | emd_24625_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Unsharpened map | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : Ternary complex of DNA Ligase with PCNA1-2-3 and end-joined DNA
Entire | Name: Ternary complex of DNA Ligase with PCNA1-2-3 and end-joined DNA |
---|---|
Components |
|
-Supramolecule #1: Ternary complex of DNA Ligase with PCNA1-2-3 and end-joined DNA
Supramolecule | Name: Ternary complex of DNA Ligase with PCNA1-2-3 and end-joined DNA type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6 |
---|---|
Source (natural) | Organism: Saccharolobus solfataricus (archaea) |
Molecular weight | Theoretical: 175 KDa |
-Macromolecule #1: DNA polymerase sliding clamp 1
Macromolecule | Name: DNA polymerase sliding clamp 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Saccharolobus solfataricus (archaea) / Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 |
Molecular weight | Theoretical: 28.711961 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MAFKIVYPNA KDFFSFINSI TNVTDSIILN FTEDGIFSRH LTEDKVLMAI MRIPKDVLSE YSIDSPTSVK LDVSSVKKIL SKASSKKAT IELTETDSGL KIIIRDEKSG AKSTIYIKAE KGQVEQLTEP KVNLAVNFTT DESVLNVIAA DVTLVGEEMR I STEEDKIK ...String: MAFKIVYPNA KDFFSFINSI TNVTDSIILN FTEDGIFSRH LTEDKVLMAI MRIPKDVLSE YSIDSPTSVK LDVSSVKKIL SKASSKKAT IELTETDSGL KIIIRDEKSG AKSTIYIKAE KGQVEQLTEP KVNLAVNFTT DESVLNVIAA DVTLVGEEMR I STEEDKIK IEAGEEGKRY VAFLMKDKPL KELSIDTSAS SSYSAEMFKD AVKGLRGFSA PTMVSFGENL PMKIDVEAVS GG HMIFWIA PRLLEHHHHH H UniProtKB: DNA polymerase sliding clamp 1 |
-Macromolecule #2: DNA polymerase sliding clamp 2
Macromolecule | Name: DNA polymerase sliding clamp 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Saccharolobus solfataricus (archaea) / Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 |
Molecular weight | Theoretical: 27.461084 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKAKVIDAVS FSYILRTVGD FLSEANFIVT KEGIRVSGID PSRVVFLDIF LPSSYFEGFE VSQEKEIIGF KLEDVNDILK RVLKDDTLI LSSNESKLTL TFDGEFTRSF ELPLIQVEST QPPSVNLEFP FKAQLLTITF ADIIDELSDL GEVLNIHSKE N KLYFEVIG ...String: MKAKVIDAVS FSYILRTVGD FLSEANFIVT KEGIRVSGID PSRVVFLDIF LPSSYFEGFE VSQEKEIIGF KLEDVNDILK RVLKDDTLI LSSNESKLTL TFDGEFTRSF ELPLIQVEST QPPSVNLEFP FKAQLLTITF ADIIDELSDL GEVLNIHSKE N KLYFEVIG DLSTAKVELS TDNGTLLEAS GADVSSSYGM EYVANTTKMR RASDSMELYF GSQIPLKLRF KLPQEGYGDF YI APRAD UniProtKB: DNA polymerase sliding clamp 2 |
-Macromolecule #3: DNA polymerase sliding clamp 3
Macromolecule | Name: DNA polymerase sliding clamp 3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Saccharolobus solfataricus (archaea) / Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 |
Molecular weight | Theoretical: 28.560268 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKVVYDDVRV LKDIIQALAR LVDEAVLKFK QDSVELVALD RAHISLISVN LPREMFKEYD VNDEFKFGFN TQYLMKILKV AKRKEAIEI ASESPDSVII NIIGSTNREF NVRNLEVSEQ EIPEINLQFD ISATISSDGF KSAISEVSTV TDNVVVEGHE D RILIKAEG ...String: MKVVYDDVRV LKDIIQALAR LVDEAVLKFK QDSVELVALD RAHISLISVN LPREMFKEYD VNDEFKFGFN TQYLMKILKV AKRKEAIEI ASESPDSVII NIIGSTNREF NVRNLEVSEQ EIPEINLQFD ISATISSDGF KSAISEVSTV TDNVVVEGHE D RILIKAEG ESEVEVEFSK DTGGLQDLEF SKESKNSYSA EYLDDVLSLT KLSDYVKISF GNQKPLQLFF NMEGGGKVTY LL APKVLEH HHHHH UniProtKB: DNA polymerase sliding clamp 3 |
-Macromolecule #6: DNA ligase
Macromolecule | Name: DNA ligase / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA ligase (ATP) |
---|---|
Source (natural) | Organism: Saccharolobus solfataricus (archaea) / Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 |
Molecular weight | Theoretical: 69.998375 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MGSSHHHHHH SSGLVPRGSH MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKTIIDK VVYIIQGKLW PDFLGYPELG IGEKFLIKA ISIATNTDEN SVENLYKTIG DLGEVARRLK SKQQSTGILG FLGTTSKESL TVDEVYSTLS KVALTTGEGS R DLKIRLLA ...String: MGSSHHHHHH SSGLVPRGSH MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKTIIDK VVYIIQGKLW PDFLGYPELG IGEKFLIKA ISIATNTDEN SVENLYKTIG DLGEVARRLK SKQQSTGILG FLGTTSKESL TVDEVYSTLS KVALTTGEGS R DLKIRLLA GLLKKADPLE AKFLVRFVEG RLRVGIGDAT VLDAMAIAFG GGQSASEIIE RAYNLRADLG NIAKIIVEKG IE ALKTLKP QVGIPIRPML AERLSNPEEI LKKMGGNAIV DYKYDGERAQ IHKKEDKIFI FSRRLENITS QYPDVVDYVS KYI EGKEFI IEGEIVAIDP ESGEMRPFQE LMHRKRKSDI YEAIKEYPVN VFLFDLMYYE DVDYTTKPLE ARRKLLESIV KPND YVKIA HHIQANNVED LKSFFYRAIS EGGEGVMVKA IGKDAIYQAG ARGWLWIKLK RDYQSEMADT VDLVVVGGFY GKGKR GGKI SSLLMAAYNP KTDSFESVCK VASGFSDEQL DELQKKLMEI KRDVKHPRVN SKMEPDIWVE PVYVAEIIGS EITISP LHT CCQDVVEKDA GLSIRFPRFI RWRDDKSPED ATTTDEILEM YNKQPKKKIE SPAVDESV UniProtKB: DNA ligase |
-Macromolecule #4: End-joined DNA
Macromolecule | Name: End-joined DNA / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 14.549299 KDa |
Sequence | String: (DG)(DT)(DA)(DT)(DC)(DC)(DT)(DC)(DG)(DT) (DA)(DG)(DT)(DG)(DC)(DA)(DG)(DA)(DT)(DG) (DC)(DG)(DT)(DC)(DG)(DT)(DC)(DG)(DG) (DA)(DC)(DT)(DG)(DA)(DT)(DT)(DC)(DG)(DG) (DT) (DA)(DG)(DA)(DT)(DC)(DT)(DG) |
-Macromolecule #5: Template strand DNA
Macromolecule | Name: Template strand DNA / type: dna / ID: 5 / Number of copies: 1 / Classification: DNA |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 14.403264 KDa |
Sequence | String: (DC)(DA)(DG)(DA)(DT)(DC)(DT)(DA)(DC)(DC) (DG)(DA)(DA)(DT)(DC)(DA)(DG)(DT)(DC)(DC) (DG)(DA)(DC)(DG)(DA)(DC)(DG)(DC)(DA) (DT)(DC)(DT)(DG)(DC)(DA)(DC)(DT)(DA)(DC) (DG) (DA)(DG)(DG)(DA)(DT)(DA)(DC) |
-Macromolecule #7: MANGANESE (II) ION
Macromolecule | Name: MANGANESE (II) ION / type: ligand / ID: 7 / Number of copies: 3 / Formula: MN |
---|---|
Molecular weight | Theoretical: 54.938 Da |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.35 mg/mL |
---|---|
Buffer | pH: 7.5 |
Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV Details: wait time 0, blot force 1, blot time 1, drain time 0. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 8060 / Average electron dose: 100.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
---|---|
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.1) / Number images used: 192490 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1) |
Final angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1) |
-Atomic model buiding 1
Initial model |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Protocol: RIGID BODY FIT | ||||||||||||
Output model | PDB-7rpx: |